bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: eggV2 2,483,276 sequences; 915,453,621 total letters Query= Eten_4731_orf2 Length=107 Score E Sequences producing significant alignments: (Bits) Value 9785.ENSLAFP00000001226 38.5 0.058 > 9785.ENSLAFP00000001226 Length=1258 Score = 38.5 bits (88), Expect = 0.058, Method: Composition-based stats. Identities = 28/74 (37%), Positives = 35/74 (47%), Gaps = 13/74 (17%) Query 23 KNPSPPRKRGKETLAPTRTPPRGGANFSPPFPKKNFGGNPPRLNGGTAPPVHPPGGKQK- 81 K+ SPP ++ AP PRGG P + +G PR NG HPPGG + Sbjct 5 KSVSPPGYAAQKRAAPA---PRGG-----PEHRSAWGEADPRANGYP----HPPGGSARG 52 Query 82 SLSNPGGKKSPQGR 95 S PGG +PQ R Sbjct 53 STKKPGGAVTPQQR 66 Lambda K H 0.310 0.139 0.445 Gapped Lambda K H 0.267 0.0410 0.140 Effective search space used: 22528463995 Database: eggV2 Posted date: Dec 15, 2009 4:47 PM Number of letters in database: 915,453,621 Number of sequences in database: 2,483,276 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 11 Window for multiple hits: 40