bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_7836_orf1
Length=165
Score E
Sequences producing significant alignments: (Bits) Value
cel:T05A12.2 tre-2; TREhalase family member (tre-2); K01194 al... 67.4 2e-11
mmu:58866 Treh, 2210412M19Rik; trehalase (brush-border membran... 63.9 2e-10
hsa:11181 TREH, MGC129621, TRE, TREA; trehalase (brush-border ... 63.2 3e-10
eco:b3519 treF, ECK3504, JW3487; cytoplasmic trehalase (EC:3.2... 61.6 1e-09
ath:AT4G24040 TRE1; TRE1 (TREHALASE 1); alpha,alpha-trehalase/... 59.7 4e-09
eco:b1197 treA, ECK1185, JW1186, osmA; periplasmic trehalase (... 58.9 8e-09
dre:795901 treh, si:ch211-147p17.2; trehalase (brush-border me... 57.0 3e-08
cel:F15A2.2 tre-4; TREhalase family member (tre-4) 57.0 3e-08
cel:F57B10.7 tre-1; TREhalase family member (tre-1); K01194 al... 51.2 1e-06
cel:W05E10.4 tre-3; TREhalase family member (tre-3); K01194 al... 43.1 4e-04
cel:C23H3.7 tre-5; TREhalase family member (tre-5); K01194 alp... 43.1 4e-04
sce:YDR001C NTH1; Neutral trehalase, degrades trehalose; requi... 33.5 0.29
dre:406685 ppp2r1a, wu:fa02h04, zgc:56296; protein phosphatase... 29.6 4.6
cpv:cgd8_4910 PAN2 family 3'5' exonuclease, UBC hydrolase (ina... 28.9 8.5
> cel:T05A12.2 tre-2; TREhalase family member (tre-2); K01194
alpha,alpha-trehalase [EC:3.2.1.28]
Length=585
Score = 67.4 bits (163), Expect = 2e-11, Method: Compositional matrix adjust.
Identities = 46/159 (28%), Positives = 77/159 (48%), Gaps = 20/159 (12%)
Query 6 LNRYLGHIAEPRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQNIRAAAESGCYF 65
L R++ + PR ES+ D L++ H + + + ++ AAAESG F
Sbjct 245 LYRFVVETSHPRPESYRED-----------LESAEHLDTLEKKCVLWGDLAAAAESGRDF 293
Query 66 SSRWVPDDVDAVTEGVMPALRSESFLPVDLNSFLYHSELYLLYFGRVLNQFDPLCEGRRA 125
SSR+ V G + + R+ +PVDLNS + + + L++ +C +
Sbjct 294 SSRFFA--VHGPYAGQLASTRTSQLIPVDLNSIICGNM-------KTLSEMYTVCGDLES 344
Query 126 PEYFLKCATDRRDAMLQLLWNDEASWWFDFDISKGRQST 164
+YF RD + Q+LWN+E + WFDFD+ +G +T
Sbjct 345 AKYFDNEYRTLRDTIRQVLWNEEHNCWFDFDVEEGNHAT 383
> mmu:58866 Treh, 2210412M19Rik; trehalase (brush-border membrane
glycoprotein) (EC:3.2.1.28); K01194 alpha,alpha-trehalase
[EC:3.2.1.28]
Length=576
Score = 63.9 bits (154), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 45/158 (28%), Positives = 72/158 (45%), Gaps = 22/158 (13%)
Query 6 LNRYLGHIAEPRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQNIRAAAESGCYF 65
LNRY PR ES+ D + + E R + ++A AESG F
Sbjct 272 LNRYYVPYGGPRPESYRKDAELANSVPE------------GDRETLWAELKAGAESGWDF 319
Query 66 SSRWVPDDVDAVTEGVMPALRSESFLPVDLNSFLYHSELYLLYFGRVLNQFDPLCEGRRA 125
SSRW+ V ++ ++R+ +P DLN+FL +E + F + L A
Sbjct 320 SSRWL---VGGPDPDLLSSIRTSKMVPADLNAFLCQAEELMSNF------YSRLGNDTEA 370
Query 126 PEYFLKCATDRRDAMLQLLWNDEASWWFDFDISKGRQS 163
+Y R AM +LW+++ WFD+D+ KG+++
Sbjct 371 TKY-RNLRAQRLAAMEAVLWDEQKGAWFDYDLEKGKKN 407
> hsa:11181 TREH, MGC129621, TRE, TREA; trehalase (brush-border
membrane glycoprotein) (EC:3.2.1.28); K01194 alpha,alpha-trehalase
[EC:3.2.1.28]
Length=583
Score = 63.2 bits (152), Expect = 3e-10, Method: Compositional matrix adjust.
Identities = 44/162 (27%), Positives = 75/162 (46%), Gaps = 22/162 (13%)
Query 2 RDLQLNRYLGHIAEPRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQNIRAAAES 61
++ LNRY PR ES+ D + T E R + ++A AES
Sbjct 271 KNYLLNRYYVPYGGPRPESYSKDVELADTLPE------------GDREALWAELKAGAES 318
Query 62 GCYFSSRWVPDDVDAVTEGVMPALRSESFLPVDLNSFLYHSELYLLYFGRVLNQFDPLCE 121
G FSSRW+ + + +R+ +PVDLN+FL +E + F + L
Sbjct 319 GWDFSSRWL---IGGPNPNSLSGIRTSKLVPVDLNAFLCQAEELMSNF------YSRLGN 369
Query 122 GRRAPEYFLKCATDRRDAMLQLLWNDEASWWFDFDISKGRQS 163
+A +Y + + R A+ +LW+++ WFD+D+ K +++
Sbjct 370 DSQATKYRI-LRSQRLAALNTVLWDEQTGAWFDYDLEKKKKN 410
> eco:b3519 treF, ECK3504, JW3487; cytoplasmic trehalase (EC:3.2.1.28);
K01194 alpha,alpha-trehalase [EC:3.2.1.28]
Length=549
Score = 61.6 bits (148), Expect = 1e-09, Method: Compositional matrix adjust.
Identities = 47/151 (31%), Positives = 72/151 (47%), Gaps = 26/151 (17%)
Query 6 LNRYLGHIAEPRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQNIRAAAESGCYF 65
LNRY PR ESW D V TA + S + + +++RA A SG +
Sbjct 281 LNRYWDDRDTPRDESWLED---VETA----------KHSGRPPNEVYRDLRAGAASGWDY 327
Query 66 SSRWVPDDVDAVTEGVMPALRSESFLPVDLNSFLYHSELYLLYFGRVLNQFDPLCEGRRA 125
SSRW+ D G + ++R+ F+P+DLN+FL+ E + L +
Sbjct 328 SSRWLRD------TGRLASIRTTQFIPIDLNAFLFKLE-------SAIANISALKGEKET 374
Query 126 PEYFLKCATDRRDAMLQLLWNDEASWWFDFD 156
F + A+ RRDA+ + LW+DE + D+D
Sbjct 375 EALFRQKASARRDAVNRYLWDDENGIYRDYD 405
> ath:AT4G24040 TRE1; TRE1 (TREHALASE 1); alpha,alpha-trehalase/
trehalase (EC:3.2.1.28); K01194 alpha,alpha-trehalase [EC:3.2.1.28]
Length=626
Score = 59.7 bits (143), Expect = 4e-09, Method: Compositional matrix adjust.
Identities = 48/162 (29%), Positives = 72/162 (44%), Gaps = 36/162 (22%)
Query 3 DLQLNRYLGHIAEPRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQ------NIR 56
D L+RY +PR ES DE SA+ S +L+ +I
Sbjct 330 DHVLSRYYAMWNKPRPESSVFDEE-----------------SASGFSTMLEKQRFHRDIA 372
Query 57 AAAESGCYFSSRWVPDDVDAVTEGVMPALRSESFLPVDLNSFLYHSELYLLYFGRVLNQF 116
AAESGC FS+RW+ D + T + + S +PVDLN FL EL + + +V
Sbjct 373 TAAESGCDFSTRWMRDPPNFTT------MATTSVVPVDLNVFLLKMELDIAFMMKV---- 422
Query 117 DPLCEGRRAPEYFLKCATDRRDAMLQLLWNDEASWWFDFDIS 158
+ + F+K + R A + WN++A W D+ +S
Sbjct 423 ---SGDQNGSDRFVKASKAREKAFQTVFWNEKAGQWLDYWLS 461
> eco:b1197 treA, ECK1185, JW1186, osmA; periplasmic trehalase
(EC:3.2.1.28); K01194 alpha,alpha-trehalase [EC:3.2.1.28]
Length=565
Score = 58.9 bits (141), Expect = 8e-09, Method: Compositional matrix adjust.
Identities = 44/156 (28%), Positives = 69/156 (44%), Gaps = 25/156 (16%)
Query 6 LNRYLGHIAEPRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQNIRAAAESGCYF 65
LNRY PR ESW D ++ N N + I +++R+AA SG F
Sbjct 266 LNRYWDDRDTPRPESWVEDIATAKS-------NPNRPATE-----IYRDLRSAAASGWDF 313
Query 66 SSRWVPDDVDAVTEGVMPALRSESFLPVDLNSFLYHSELYLLYFGRVLNQFDPLCEGRRA 125
SSRW+ + T LR+ S +PVDLNS ++ E ++L +
Sbjct 314 SSRWMDNPQQLNT------LRTTSIVPVDLNSLMFKME-------KILARASKAAGDNAM 360
Query 126 PEYFLKCATDRRDAMLQLLWNDEASWWFDFDISKGR 161
+ A R+ + + LWND+ W+ D+D+ +
Sbjct 361 ANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHK 396
> dre:795901 treh, si:ch211-147p17.2; trehalase (brush-border
membrane glycoprotein); K01194 alpha,alpha-trehalase [EC:3.2.1.28]
Length=577
Score = 57.0 bits (136), Expect = 3e-08, Method: Compositional matrix adjust.
Identities = 44/152 (28%), Positives = 67/152 (44%), Gaps = 20/152 (13%)
Query 6 LNRYLGHIAEPRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQNIRAAAESGCYF 65
LNRY + PR ES+ D + S A+ + ++ + AESG F
Sbjct 271 LNRYNVPVDHPRPESYSDDVELAEG------------LSTEAQKQLWMDLTSGAESGWDF 318
Query 66 SSRWVPDDVDAVTEGVMPALRSESFLPVDLNSFLYHSELYLLYFGRVLNQFDPLCEGRRA 125
SSRW DD G + ++ S LP DLN+ + +E L F R L + +
Sbjct 319 SSRWFIDDT-GRNNGSLRDTQTSSILPADLNAIMCRNERLLASFHRSLGNEEKALK---- 373
Query 126 PEYFLKCATDRRDAMLQLLWNDEASWWFDFDI 157
+ K + R A+ LLW+ E WFD+++
Sbjct 374 ---YEKALSARIKAVESLLWDAEKGAWFDYNL 402
> cel:F15A2.2 tre-4; TREhalase family member (tre-4)
Length=635
Score = 57.0 bits (136), Expect = 3e-08, Method: Compositional matrix adjust.
Identities = 39/127 (30%), Positives = 66/127 (51%), Gaps = 14/127 (11%)
Query 33 EKALQNGNHEFSAAARSCILQNIRAAAESGCYFSSRWVPD-DVDAVTEGVMPALRSESFL 91
E L + N +A S + ++I +A ESG FSSRW+ + D D + ++ ++ +
Sbjct 298 ENFLGDFNIGKAAKTPSDVWRSISSACESGWDFSSRWMHNNDTD------LSSIHTDLII 351
Query 92 PVDLNSFLYHSELYLLYFGRVLNQFDPLCEGRRAPEYFLKCATDRRDAMLQLLWNDEASW 151
PVDLN F+ ++ Y+ Y+ +FD R+ E R A+ ++LW++
Sbjct 352 PVDLNVFIANNYRYMAYYANHFGRFDKSASYRQKYEKL-------RYAIQEVLWDNNLGA 404
Query 152 WFDFDIS 158
WFD+DIS
Sbjct 405 WFDYDIS 411
> cel:F57B10.7 tre-1; TREhalase family member (tre-1); K01194
alpha,alpha-trehalase [EC:3.2.1.28]
Length=567
Score = 51.2 bits (121), Expect = 1e-06, Method: Composition-based stats.
Identities = 39/158 (24%), Positives = 69/158 (43%), Gaps = 20/158 (12%)
Query 8 RYLGHIAEPRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQNIRAAAESGCYFSS 67
+Y + PR ES+ D + H + A + + I +AAE+G FS+
Sbjct 256 QYKAKLKVPRPESYREDSEL-----------AEHLQTEAEKIQMWSEIASAAETGWDFST 304
Query 68 RWVPDDVDAVTEGVMPALRSESFLPVDLNSFLYHSELYLLYFGRVLNQFDPLCEGRRAPE 127
RW + D + M ++R+ S +P DLN+F+ + R+L + + +
Sbjct 305 RWFSQNGDTMHR--MDSIRTWSIIPADLNAFMCAN-------ARILASLYEIAGDFKKVK 355
Query 128 YFLKCATDRRDAMLQLLWNDEASWWFDFDISKGRQSTR 165
F + T + M +L WN+ W+D+DI S +
Sbjct 356 VFEQRYTWAKREMRELHWNETDGIWYDYDIELKTHSNQ 393
> cel:W05E10.4 tre-3; TREhalase family member (tre-3); K01194
alpha,alpha-trehalase [EC:3.2.1.28]
Length=588
Score = 43.1 bits (100), Expect = 4e-04, Method: Composition-based stats.
Identities = 37/150 (24%), Positives = 64/150 (42%), Gaps = 24/150 (16%)
Query 16 PRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQNIRAAAESGCYFSSRWVPDDVD 75
PR ES+ D T L NG A + Q++ +AAESG FS+RW D
Sbjct 273 PRPESYRVD-----TQNSAKLANG------ADQQQFYQDLASAAESGWDFSTRWFSD--- 318
Query 76 AVTEGVMPALRSESFLPVDLNSFLYHSELYLLYFGRVLNQFDPLCEGRRAPEYFLKCATD 135
+ ++ + LPVDLN L + + Y ++ + + + + + F D
Sbjct 319 ---YKTLTSIETTKVLPVDLNGLLCWNMDIMEYL------YEQIGDTKNS-QIFRNKRAD 368
Query 136 RRDAMLQLLWNDEASWWFDFDISKGRQSTR 165
RD + + +N W+D+++ + R
Sbjct 369 FRDTVQNVFYNRTDGTWYDYNLRTQSHNPR 398
> cel:C23H3.7 tre-5; TREhalase family member (tre-5); K01194 alpha,alpha-trehalase
[EC:3.2.1.28]
Length=674
Score = 43.1 bits (100), Expect = 4e-04, Method: Composition-based stats.
Identities = 39/155 (25%), Positives = 69/155 (44%), Gaps = 26/155 (16%)
Query 4 LQLNRYLGHIAEPRTESWDTDETVVRTAAEKALQNGNHEFSAAARSCILQNIRAAAESGC 63
L + +Y PR ES+ D V +A ++ +F +++ +AAESG
Sbjct 329 LHMFQYRTEAETPRPESFRED---VLSAEHFTTKDRKKQF--------FKDLGSAAESGW 377
Query 64 YFSSRWVPDDVDAVTEGVMPALRSESFLPVDLNSFL-YHSELYLLYFGRVLNQFDPLCEG 122
FSSRW + D T + + + +PVDLN+FL Y+ + L++ N L
Sbjct 378 DFSSRWFKNHKDIST------IETTNIVPVDLNAFLCYNMNIMQLFYKLTGNPLKHLEWS 431
Query 123 RRAPEYFLKCATDRRDAMLQLLWNDEASWWFDFDI 157
R T+ R+A ++ + W+D+++
Sbjct 432 SR--------FTNFREAFTKVFYVPARKGWYDYNL 458
> sce:YDR001C NTH1; Neutral trehalase, degrades trehalose; required
for thermotolerance and may mediate resistance to other
cellular stresses; may be phosphorylated by Cdc28p (EC:3.2.1.28);
K01194 alpha,alpha-trehalase [EC:3.2.1.28]
Length=751
Score = 33.5 bits (75), Expect = 0.29, Method: Compositional matrix adjust.
Identities = 19/70 (27%), Positives = 37/70 (52%), Gaps = 9/70 (12%)
Query 93 VDLNSFLYHSELYLLYFGRVLNQF------DPLCEGRRAPEYFLKCATDRRDAMLQLLWN 146
+DLNS LY E+ + F + +F DPL + + A R++ + + +W+
Sbjct 493 IDLNSLLYKYEIDIADF---IKEFCDDKYEDPLDHSITTSAMWKEMAKIRQEKITKYMWD 549
Query 147 DEASWWFDFD 156
DE+ ++FD++
Sbjct 550 DESGFFFDYN 559
> dre:406685 ppp2r1a, wu:fa02h04, zgc:56296; protein phosphatase
2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform;
K03456 protein phosphatase 2 (formerly 2A), regulatory
subunit A
Length=476
Score = 29.6 bits (65), Expect = 4.6, Method: Compositional matrix adjust.
Identities = 24/87 (27%), Positives = 37/87 (42%), Gaps = 6/87 (6%)
Query 12 HIAEPRTESWDT-DETVVRTAAEKALQNGNHEFSAAA-----RSCILQNIRAAAESGCYF 65
H P ES T +ETVVR A ++L+ +HE S ++ +R AAE +
Sbjct 87 HCLLPPLESLATVEETVVRDKAVESLRKISHEHSPVDLEVHFEPLVMPTLRQAAEDKSWR 146
Query 66 SSRWVPDDVDAVTEGVMPALRSESFLP 92
V D + + V P + +P
Sbjct 147 VRYMVADKFSDLQKAVGPEITKNDLVP 173
> cpv:cgd8_4910 PAN2 family 3'5' exonuclease, UBC hydrolase (inactive)
plus nuclease ; K12571 PAB-dependent poly(A)-specific
ribonuclease subunit 2 [EC:3.1.13.4]
Length=1936
Score = 28.9 bits (63), Expect = 8.5, Method: Composition-based stats.
Identities = 31/120 (25%), Positives = 51/120 (42%), Gaps = 16/120 (13%)
Query 47 ARSCILQNIRAAAESGCYFSSRWVPDDVDAVTEGVMPALRSESFLPVDLNSFLYHSELYL 106
++SCIL+ ++ ++G F +P D + V P ++ L Y S +L
Sbjct 1809 SKSCILKKLQFLVDAGVVFIGHALPSDFKIINIYVPPFQIIDTVEIYRLPDERYISLKFL 1868
Query 107 LYFGRVLNQ------FDPLCEGRRAPEYFLKCATDRRDAMLQLLWNDEASWWFDFDISKG 160
F VLN+ D + + + A E FLK + ++ WND + F SKG
Sbjct 1869 AKF--VLNKNIQTEVHDSIVDAKTALELFLKHLSLKKSGS----WND----FLSFLYSKG 1918
Lambda K H
0.322 0.135 0.426
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 3962792044
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40