bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_7398_orf2
Length=143
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_032730 diacylglycerol acyltransferase, putative (EC... 231 5e-61
tpv:TP01_0247 diacylglycerol O-acyltransferase; K00635 diacylg... 193 2e-49
pfa:PFC0995c acyl CoA:diacylglycerol acyltransferase, putative... 182 3e-46
bbo:BBOV_IV004970 23.m06462; diacylglycerol acyltransferase (E... 180 1e-45
cpv:cgd2_1090 diacylglycerol acyltransferase 1 (EC:2.3.1.20); ... 177 8e-45
ath:AT2G19450 TAG1; TAG1 (TRIACYLGLYCEROL BIOSYNTHESIS DEFECT ... 124 1e-28
mmu:13350 Dgat1, C75990, D15Ertd23e, Dgat; diacylglycerol O-ac... 99.8 3e-21
hsa:8694 DGAT1, ARGP1, DGAT; diacylglycerol O-acyltransferase ... 99.8 3e-21
dre:436731 dgat1b, zgc:92327; diacylglycerol O-acyltransferase... 99.0 5e-21
cel:H19N07.4 hypothetical protein; K11155 diacylglycerol O-acy... 94.0 2e-19
dre:325875 dgat1a, dgat1, wu:fd36g11, zgc:77691; diacylglycero... 92.0 6e-19
sce:YNR019W ARE2, SAT1; Acyl-CoA:sterol acyltransferase, isozy... 79.0 4e-15
tgo:TGME49_063710 sterol O-acyltransferase, putative (EC:2.3.1... 68.6 6e-12
sce:YCR048W ARE1, SAT2; Acyl-CoA:sterol acyltransferase, isozy... 64.3 1e-10
xla:779411 soat2, MGC154806; sterol O-acyltransferase 2 (EC:2.... 62.4 5e-10
dre:564424 novel protein similar to vertebrate sterol O-acyltr... 58.2 9e-09
hsa:8435 SOAT2, ACACT2, ACAT2, ARGP2, MGC116732; sterol O-acyl... 57.8 1e-08
mmu:20652 Soat1, 8430426K15Rik, ACAT-1, AW550831, Acact, ald; ... 57.4 1e-08
hsa:6646 SOAT1, ACACT, ACAT, ACAT1, RP11-215I23.2, SOAT, STAT;... 55.8 5e-08
mmu:223920 Soat2, ACAT2, D15Wsu97e, MGC36039; sterol O-acyltra... 55.5 6e-08
cel:B0395.2 hypothetical protein; K00637 sterol O-acyltransfer... 55.5 6e-08
xla:446863 soat1, MGC80837; sterol O-acyltransferase 1 (EC:2.3... 52.4 5e-07
dre:564868 hypothetical LOC564868 43.9 1e-04
xla:397828 porcn, Xporc, xporc-d; porcupine homolog; K00181 po... 34.7 0.094
cel:T07H6.2 mom-1; More Of MS family member (mom-1); K00181 po... 32.3 0.51
mmu:53627 Porcn, 2410004O13Rik, AW045557, DXHXS7465e, Mporc, M... 31.6 0.90
hsa:64840 PORCN, DHOF, FODH, MG61, MGC29687, PORC, PPN, por; p... 31.6 0.94
ath:AT1G34520 long-chain-alcohol O-fatty-acyltransferase famil... 31.2 1.0
ath:AT5G55380 membrane bound O-acyl transferase (MBOAT) family... 30.8 1.5
pfa:MAL13P1.93 conserved Plasmodium protein, unknown function 29.6 3.6
dre:334395 slc43a1a, fi47a12, slc43a1, wu:fi47a12, zgc:55850; ... 28.9 6.2
dre:100006275 novel protein similar to vertebrate porcupine ho... 28.5 6.4
ath:AT1G12840 DET3; DET3 (DE-ETIOLATED 3); proton-transporting... 28.5 6.8
dre:100148644 porcupine-like; K00181 porcupine 28.5 7.9
dre:333999 PORCN, fi26a04, wu:fi26a04; zgc:55392; K00181 porcu... 28.1 8.4
> tgo:TGME49_032730 diacylglycerol acyltransferase, putative (EC:2.3.1.20);
K00635 diacylglycerol O-acyltransferase [EC:2.3.1.20]
Length=540
Score = 231 bits (590), Expect = 5e-61, Method: Compositional matrix adjust.
Identities = 101/142 (71%), Positives = 118/142 (83%), Gaps = 0/142 (0%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DR+FYLDWWNASSFGEYWRKWNLPIH FL+RH+NKPL R P+ A VVF ISAL+HE
Sbjct 398 DRQFYLDWWNASSFGEYWRKWNLPIHHFLNRHINKPLRRARFPRIVATSVVFLISALLHE 457
Query 61 YLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLGILL 120
Y++ VPLQLGWTG++F F+ Q PL Y TN+ FFQ NPTVGNCFFW +FCF+GQPLGIL+
Sbjct 458 YMITVPLQLGWTGWVFLGFMAQAPLTYITNLSFFQRNPTVGNCFFWFIFCFSGQPLGILI 517
Query 121 YWYLWGVKQGAVTELDPSRIAL 142
YWYLWGVK G V +LDPS+I +
Sbjct 518 YWYLWGVKHGTVQQLDPSKIQI 539
> tpv:TP01_0247 diacylglycerol O-acyltransferase; K00635 diacylglycerol
O-acyltransferase [EC:2.3.1.20]
Length=518
Score = 193 bits (490), Expect = 2e-49, Method: Compositional matrix adjust.
Identities = 86/131 (65%), Positives = 105/131 (80%), Gaps = 0/131 (0%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DR FY DWWNAS FGEYWRKWNLPIH FL RH++KPL G+P+ VVF ISA +HE
Sbjct 386 DRRFYGDWWNASCFGEYWRKWNLPIHQFLIRHISKPLTNLGLPRGLINIVVFVISAALHE 445
Query 61 YLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLGILL 120
YL+ VPL LGWTG++F+A +GQIPL+ FT++ ++N T+GN FW +FCFTGQPLG+LL
Sbjct 446 YLISVPLGLGWTGYVFWAMMGQIPLLLFTDMDVIKNNKTLGNVLFWCLFCFTGQPLGVLL 505
Query 121 YWYLWGVKQGA 131
YWYLWGVKQG+
Sbjct 506 YWYLWGVKQGS 516
> pfa:PFC0995c acyl CoA:diacylglycerol acyltransferase, putative
(EC:2.3.1.20); K00635 diacylglycerol O-acyltransferase [EC:2.3.1.20]
Length=653
Score = 182 bits (462), Expect = 3e-46, Method: Composition-based stats.
Identities = 81/132 (61%), Positives = 107/132 (81%), Gaps = 0/132 (0%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DR FY DWWNASSF EYWRKWNLPI++F+ RH+NKPL+ G+ + + +VFFISA++HE
Sbjct 520 DRLFYKDWWNASSFAEYWRKWNLPIYYFVCRHINKPLIYYGIHRNVSMIIVFFISAMLHE 579
Query 61 YLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLGILL 120
YL+ +PL+LG+TG+IFFAFI QIPL+ FTN +F+ + T+GN FW+VFCFTGQPL + +
Sbjct 580 YLISIPLKLGFTGYIFFAFICQIPLVQFTNNAYFKKHKTIGNSIFWIVFCFTGQPLILFI 639
Query 121 YWYLWGVKQGAV 132
Y+YLW KQG +
Sbjct 640 YYYLWIDKQGTL 651
> bbo:BBOV_IV004970 23.m06462; diacylglycerol acyltransferase
(EC:2.3.1.20); K00635 diacylglycerol O-acyltransferase [EC:2.3.1.20]
Length=542
Score = 180 bits (457), Expect = 1e-45, Method: Compositional matrix adjust.
Identities = 83/131 (63%), Positives = 99/131 (75%), Gaps = 0/131 (0%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DR FY DWWNAS+FGEYWRKWNLPIH F+ RH++KPL GVP VVF +SA +HE
Sbjct 410 DRRFYGDWWNASNFGEYWRKWNLPIHQFIVRHISKPLHTIGVPWEITKVVVFMLSAALHE 469
Query 61 YLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLGILL 120
YL+ VPL LGWTG++F+A +GQIPL+ T + T GN FW +FCFTGQPLG+LL
Sbjct 470 YLISVPLGLGWTGYVFWAMMGQIPLLMVTRFTPIRMGRTTGNVLFWFLFCFTGQPLGVLL 529
Query 121 YWYLWGVKQGA 131
YWYLWGVKQG+
Sbjct 530 YWYLWGVKQGS 540
> cpv:cgd2_1090 diacylglycerol acyltransferase 1 (EC:2.3.1.20);
K00635 diacylglycerol O-acyltransferase [EC:2.3.1.20]
Length=505
Score = 177 bits (450), Expect = 8e-45, Method: Compositional matrix adjust.
Identities = 77/140 (55%), Positives = 103/140 (73%), Gaps = 0/140 (0%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DR FYLDWWNA +FGEYWRKWNLP+HFFL RH+ KPL + G F+ +VF SAL+HE
Sbjct 339 DRAFYLDWWNAGNFGEYWRKWNLPVHFFLTRHIFKPLRKKGFSSSFSGLLVFTFSALIHE 398
Query 61 YLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLGILL 120
YLVVVPL +GWTG++ AF+ Q+PL +T + Q T+GN FW+ FCF GQP+ +LL
Sbjct 399 YLVVVPLDIGWTGWVVLAFVFQVPLSSWTQSSYIQKRETLGNVLFWITFCFCGQPIAVLL 458
Query 121 YWYLWGVKQGAVTELDPSRI 140
Y+YLW + +G + ++D ++I
Sbjct 459 YYYLWALSKGQIDQIDLNKI 478
> ath:AT2G19450 TAG1; TAG1 (TRIACYLGLYCEROL BIOSYNTHESIS DEFECT
1); diacylglycerol O-acyltransferase (EC:2.3.1.20); K11155
diacylglycerol O-acyltransferase 1 [EC:2.3.1.20 2.3.1.75 2.3.1.76]
Length=520
Score = 124 bits (310), Expect = 1e-28, Method: Compositional matrix adjust.
Identities = 59/136 (43%), Positives = 88/136 (64%), Gaps = 7/136 (5%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DREFY DWWNA S G+YWR WN+P+H ++ RH+ P LR+ +PK A + F +SA+ HE
Sbjct 389 DREFYKDWWNAKSVGDYWRMWNMPVHKWMVRHIYFPCLRSKIPKTLAIIIAFLVSAVFHE 448
Query 61 YLVVVPLQLGWTGFIFFAFIG---QIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLG 117
+ VP +L F +AF+G Q+PL++ TN + TVGN FW +FC GQP+
Sbjct 449 LCIAVPCRL----FKLWAFLGIMFQVPLVFITNYLQERFGSTVGNMIFWFIFCIFGQPMC 504
Query 118 ILLYWYLWGVKQGAVT 133
+LLY++ ++G+++
Sbjct 505 VLLYYHDLMNRKGSMS 520
> mmu:13350 Dgat1, C75990, D15Ertd23e, Dgat; diacylglycerol O-acyltransferase
1 (EC:2.3.1.20); K11155 diacylglycerol O-acyltransferase
1 [EC:2.3.1.20 2.3.1.75 2.3.1.76]
Length=498
Score = 99.8 bits (247), Expect = 3e-21, Method: Compositional matrix adjust.
Identities = 54/121 (44%), Positives = 74/121 (61%), Gaps = 5/121 (4%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DREFY DWWNA S +W+ WN+P+H + RH KP+LR+G K+ A VF SA HE
Sbjct 368 DREFYRDWWNAESVTYFWQNWNIPVHKWCIRHFYKPMLRHGSSKWVARTGVFLTSAFFHE 427
Query 61 YLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLGILL 120
YLV VPL++ + + F A + Q+PL + +FFQ N GN W+ GQP+ +L+
Sbjct 428 YLVSVPLRM-FRLWAFTAMMAQVPLAWIVG-RFFQGN--YGNAAVWVTL-IIGQPVAVLM 482
Query 121 Y 121
Y
Sbjct 483 Y 483
> hsa:8694 DGAT1, ARGP1, DGAT; diacylglycerol O-acyltransferase
1 (EC:2.3.1.20); K11155 diacylglycerol O-acyltransferase 1
[EC:2.3.1.20 2.3.1.75 2.3.1.76]
Length=488
Score = 99.8 bits (247), Expect = 3e-21, Method: Compositional matrix adjust.
Identities = 58/124 (46%), Positives = 74/124 (59%), Gaps = 11/124 (8%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DREFY DWWN+ S +W+ WN+P+H + RH KP+LR G K+ A VF SA HE
Sbjct 357 DREFYRDWWNSESVTYFWQNWNIPVHKWCIRHFYKPMLRRGSSKWMARTGVFLASAFFHE 416
Query 61 YLVVVPLQLGWTGFIFFAFIG---QIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLG 117
YLV VPL++ F +AF G QIPL +F +FFQ N GN WL GQP+
Sbjct 417 YLVSVPLRM----FRLWAFTGMMAQIPLAWFVG-RFFQGN--YGNAAVWLSL-IIGQPIA 468
Query 118 ILLY 121
+L+Y
Sbjct 469 VLMY 472
> dre:436731 dgat1b, zgc:92327; diacylglycerol O-acyltransferase
homolog 1b (mouse)
Length=507
Score = 99.0 bits (245), Expect = 5e-21, Method: Compositional matrix adjust.
Identities = 52/121 (42%), Positives = 72/121 (59%), Gaps = 5/121 (4%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DREFY DWWN+ + +W+ WN+P+H + RH KPLLR G K + VFF SA HE
Sbjct 373 DREFYRDWWNSETITYFWQNWNIPVHKWCLRHFYKPLLRRGAGKLLSQSAVFFASAFFHE 432
Query 61 YLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLGILL 120
YLV VPL++ + + F + QIPL + + +F + N GN WL GQP+ +L+
Sbjct 433 YLVSVPLRM-FRLWAFMGMMAQIPLAWLVS-RFLRGN--YGNAAVWLSL-IIGQPIAVLM 487
Query 121 Y 121
Y
Sbjct 488 Y 488
> cel:H19N07.4 hypothetical protein; K11155 diacylglycerol O-acyltransferase
1 [EC:2.3.1.20 2.3.1.75 2.3.1.76]
Length=496
Score = 94.0 bits (232), Expect = 2e-19, Method: Compositional matrix adjust.
Identities = 57/142 (40%), Positives = 84/142 (59%), Gaps = 4/142 (2%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DREFY D+WNA + G +W+ WN+P+H F RH+ P++RN K A VVFF+SA HE
Sbjct 359 DREFYRDFWNAETIGYFWKSWNIPVHRFAVRHIYSPMMRNNFSKMSAFFVVFFVSAFFHE 418
Query 61 YLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLGILL 120
YLV VPL++ + + ++ +GQIPL T+ + + T GN WL GQPL IL+
Sbjct 419 YLVSVPLKI-FRLWSYYGMMGQIPLSIITD-KVVRGGRT-GNIIVWLSL-IVGQPLAILM 474
Query 121 YWYLWGVKQGAVTELDPSRIAL 142
Y + W + V+ + + +
Sbjct 475 YGHDWYILNFGVSAVQNQTVGI 496
> dre:325875 dgat1a, dgat1, wu:fd36g11, zgc:77691; diacylglycerol
O-acyltransferase homolog 1a (mouse) (EC:2.3.1.20); K11155
diacylglycerol O-acyltransferase 1 [EC:2.3.1.20 2.3.1.75
2.3.1.76]
Length=499
Score = 92.0 bits (227), Expect = 6e-19, Method: Compositional matrix adjust.
Identities = 50/124 (40%), Positives = 71/124 (57%), Gaps = 11/124 (8%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
DREFY DWWN+ + +W WN+P+H + RH KP+L G + A VFF+SA HE
Sbjct 366 DREFYRDWWNSETIPYFWSNWNIPVHKWCLRHFYKPMLMRGANRLAAQIAVFFLSAFFHE 425
Query 61 YLVVVPLQLGWTGFIFFAFIG---QIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLG 117
YLV VPL++ F +AF+G Q+PL ++ + N GN W+ GQP+
Sbjct 426 YLVSVPLKM----FRLWAFLGMMLQVPLAILVG-RYLRGN--YGNAAVWMSL-IIGQPIA 477
Query 118 ILLY 121
+L+Y
Sbjct 478 VLMY 481
> sce:YNR019W ARE2, SAT1; Acyl-CoA:sterol acyltransferase, isozyme
of Are1p; endoplasmic reticulum enzyme that contributes
the major sterol esterification activity in the presence of
oxygen (EC:2.3.1.26); K00637 sterol O-acyltransferase [EC:2.3.1.26]
Length=642
Score = 79.0 bits (193), Expect = 4e-15, Method: Composition-based stats.
Identities = 42/114 (36%), Positives = 67/114 (58%), Gaps = 8/114 (7%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRN-GVPKFFAACVVFFISALMH 59
DR FY DWWN S+ ++ R WN+P+H FL RHV + + + K A + FF+S+++H
Sbjct 520 DRYFYGDWWNCVSWADFSRIWNIPVHKFLLRHVYHSSMSSFKLNKSQATLMTFFLSSVVH 579
Query 60 E---YLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFC 110
E Y++ L+ ++FF + Q+PL+ TN +F ++ +GN FWL C
Sbjct 580 ELAMYVIFKKLRF----YLFFFQMLQMPLVALTNTKFMRNRTIIGNVIFWLGIC 629
> tgo:TGME49_063710 sterol O-acyltransferase, putative (EC:2.3.1.26);
K00637 sterol O-acyltransferase [EC:2.3.1.26]
Length=578
Score = 68.6 bits (166), Expect = 6e-12, Method: Composition-based stats.
Identities = 39/107 (36%), Positives = 58/107 (54%), Gaps = 4/107 (3%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVN-KPLLRNGVPKFFAACVVFFISALMH 59
+R FY DWWN++++ EY RKWN P+H FL RHV + R AA F SAL+H
Sbjct 439 NRNFYDDWWNSTNWDEYSRKWNKPVHRFLLRHVYMETQQRYKWSHQTAAFATFLFSALLH 498
Query 60 EYLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFW 106
E ++ V + ++F + Q+PL+ +F++ V N FW
Sbjct 499 EMILAVCFRFVRL-YLFGLMLLQLPLIALG--RFYRHKKMVANAIFW 542
> sce:YCR048W ARE1, SAT2; Acyl-CoA:sterol acyltransferase, isozyme
of Are2p; endoplasmic reticulum enzyme that contributes
the major sterol esterification activity in the absence of
oxygen (EC:2.3.1.26); K00637 sterol O-acyltransferase [EC:2.3.1.26]
Length=610
Score = 64.3 bits (155), Expect = 1e-10, Method: Compositional matrix adjust.
Identities = 42/125 (33%), Positives = 63/125 (50%), Gaps = 3/125 (2%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHV-NKPLLRNGVPKFFAACVVFFISALMH 59
DR FY DWWN SF E+ R WN+P+H FL RHV + + + K A FF+SA+ H
Sbjct 488 DRYFYGDWWNCVSFEEFSRIWNVPVHKFLLRHVYHSSMGALHLSKSQATLFTFFLSAVFH 547
Query 60 EYLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLGIL 119
E + + + G++F + Q +N +F + P + N F C +G + +
Sbjct 548 E-MAMFAIFRRVRGYLFMFQLSQFVWTALSNTKFLRARPQLSNVVFSFGVC-SGPSIIMT 605
Query 120 LYWYL 124
LY L
Sbjct 606 LYLTL 610
> xla:779411 soat2, MGC154806; sterol O-acyltransferase 2 (EC:2.3.1.26);
K00637 sterol O-acyltransferase [EC:2.3.1.26]
Length=532
Score = 62.4 bits (150), Expect = 5e-10, Method: Compositional matrix adjust.
Identities = 44/128 (34%), Positives = 66/128 (51%), Gaps = 8/128 (6%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACV--VFFISALM 58
DR FY DWWN++SF Y+R WN+ +H +L+ +V + LL ++ A + VF ISA +
Sbjct 384 DRMFYKDWWNSTSFSNYYRTWNVVVHDWLYYYVYQDLLSLCKQRYRAGVMLTVFLISASV 443
Query 59 HEYLVVVPLQLGW-TGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLG 117
HEY+ + L+ + F FA G + F + + V N W F F GQ +
Sbjct 444 HEYVFTLSLRYFYPVMFCLFAIFGVL----FNFMMNDKRKSPVWNVMVW-TFLFIGQGIQ 498
Query 118 ILLYWYLW 125
+ LY W
Sbjct 499 LCLYSQEW 506
> dre:564424 novel protein similar to vertebrate sterol O-acyltransferase
(acyl-Coenzyme A: cholesterol acyltransferase) 1
(SOAT1) (EC:2.3.1.26); K00637 sterol O-acyltransferase [EC:2.3.1.26]
Length=554
Score = 58.2 bits (139), Expect = 9e-09, Method: Compositional matrix adjust.
Identities = 46/145 (31%), Positives = 71/145 (48%), Gaps = 9/145 (6%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAAC--VVFFISALM 58
DR FY DWWN++S+ Y+R WN+ +H +L+ +V + L +F A VVF +SA++
Sbjct 397 DRMFYKDWWNSTSYANYYRTWNVVVHDWLYYYVYRDFLWMTQKRFRAVAMLVVFTVSAVV 456
Query 59 HEYLVVVPLQLGW-TGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLG 117
HEY++ + + F F G M F + + + N W F GQ +
Sbjct 457 HEYVLAICFGFFYPVMFCLFMCFG----MVFNFVLHDRRKGPIWNVIMWTSL-FLGQGVM 511
Query 118 ILLYWYLWGVKQGAVTELDPSRIAL 142
I LY W ++ E +PS I L
Sbjct 512 ICLYSQEWYAQRYCPIE-EPSLIDL 535
> hsa:8435 SOAT2, ACACT2, ACAT2, ARGP2, MGC116732; sterol O-acyltransferase
2 (EC:2.3.1.26); K00637 sterol O-acyltransferase
[EC:2.3.1.26]
Length=522
Score = 57.8 bits (138), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 42/135 (31%), Positives = 64/135 (47%), Gaps = 14/135 (10%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLR--NGVPKFFAACVVFFISALM 58
DR FY DWWN++SF Y+R WN+ +H +L+ +V + LR + A VF +SA+
Sbjct 374 DRMFYRDWWNSTSFSNYYRTWNVVVHDWLYSYVYQDGLRLLGARARGVAMLGVFLVSAVA 433
Query 59 HEYLVVVPLQLGW-TGFIFFAFIGQIPLMYFTNIQFFQDNPTVG---NCFFWLVFCFTGQ 114
HEY+ L + I F IG + + F + G N W + F GQ
Sbjct 434 HEYIFCFVLGFFYPVMLILFLVIGGM-------LNFMMHDQRTGPAWNVLMWTML-FLGQ 485
Query 115 PLGILLYWYLWGVKQ 129
+ + LY W ++
Sbjct 486 GIQVSLYCQEWYARR 500
> mmu:20652 Soat1, 8430426K15Rik, ACAT-1, AW550831, Acact, ald;
sterol O-acyltransferase 1 (EC:2.3.1.26); K00637 sterol O-acyltransferase
[EC:2.3.1.26]
Length=540
Score = 57.4 bits (137), Expect = 1e-08, Method: Compositional matrix adjust.
Identities = 33/84 (39%), Positives = 52/84 (61%), Gaps = 3/84 (3%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACV--VFFISALM 58
DR FY DWWN++S+ Y+R WN+ +H +L+ +V K LL +F +A + VF +SA++
Sbjct 390 DRMFYKDWWNSTSYSNYYRTWNVVVHDWLYYYVYKDLLWFFSKRFKSAAMLAVFALSAVV 449
Query 59 HEYLVVVPLQLGW-TGFIFFAFIG 81
HEY + + L + F+ F F G
Sbjct 450 HEYALAICLSYFYPVLFVLFMFFG 473
> hsa:6646 SOAT1, ACACT, ACAT, ACAT1, RP11-215I23.2, SOAT, STAT;
sterol O-acyltransferase 1 (EC:2.3.1.26); K00637 sterol O-acyltransferase
[EC:2.3.1.26]
Length=550
Score = 55.8 bits (133), Expect = 5e-08, Method: Compositional matrix adjust.
Identities = 42/132 (31%), Positives = 64/132 (48%), Gaps = 8/132 (6%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACV--VFFISALM 58
DR FY DWWN++S+ Y+R WN+ +H +L+ + K L +F +A + VF +SA++
Sbjct 400 DRMFYKDWWNSTSYSNYYRTWNVVVHDWLYYYAYKDFLWFFSKRFKSAAMLAVFAVSAVV 459
Query 59 HEYLVVVPLQLGW-TGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLG 117
HEY + V L + F+ F F G M F I + N W F G +
Sbjct 460 HEYALAVCLSFFYPVLFVLFMFFG----MAFNFIVNDSRKKPIWNVLMWTSL-FLGNGVL 514
Query 118 ILLYWYLWGVKQ 129
+ Y W +Q
Sbjct 515 LCFYSQEWYARQ 526
> mmu:223920 Soat2, ACAT2, D15Wsu97e, MGC36039; sterol O-acyltransferase
2 (EC:2.3.1.26); K00637 sterol O-acyltransferase
[EC:2.3.1.26]
Length=525
Score = 55.5 bits (132), Expect = 6e-08, Method: Compositional matrix adjust.
Identities = 42/134 (31%), Positives = 68/134 (50%), Gaps = 12/134 (8%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPL--LRNGVPKFFAACVVFFISALM 58
DR FY DWWN++SF Y+R WN+ +H +L+ +V + L + A VF +SA++
Sbjct 377 DRMFYRDWWNSTSFSNYYRTWNVVVHDWLYSYVYQDGLWLLGRRARGVAMLGVFLVSAVV 436
Query 59 HEYLVVVPLQLGWTGFIFFAFIGQIPLMYFTNIQFFQDNPTVG---NCFFWLVFCFTGQP 115
HEY+ L GF F+ + + L++ + F ++ G N W F F GQ
Sbjct 437 HEYIFCFVL-----GF-FYPVMLMLFLVFGGLLNFTMNDRHTGPAWNILMW-TFLFMGQG 489
Query 116 LGILLYWYLWGVKQ 129
+ + LY W ++
Sbjct 490 IQVSLYCQEWYARR 503
> cel:B0395.2 hypothetical protein; K00637 sterol O-acyltransferase
[EC:2.3.1.26]
Length=467
Score = 55.5 bits (132), Expect = 6e-08, Method: Compositional matrix adjust.
Identities = 35/93 (37%), Positives = 51/93 (54%), Gaps = 16/93 (17%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLL-----RNGVPKFFAACVVFFIS 55
DR+FYL+WW++S+ EY+R WNL +H +L+ +V + L R G A VFF+S
Sbjct 314 DRQFYLNWWHSSNMAEYYRNWNLVVHDWLYAYVFRDLAAYNPGRKGQRA--AQMAVFFLS 371
Query 56 ALMHEYLVVVPLQLGWTGFIFFAFIGQIPLMYF 88
A+ HEY W G F F + ++YF
Sbjct 372 AVFHEY---------WFGVAFRCFYPVMFVLYF 395
> xla:446863 soat1, MGC80837; sterol O-acyltransferase 1 (EC:2.3.1.26);
K00637 sterol O-acyltransferase [EC:2.3.1.26]
Length=550
Score = 52.4 bits (124), Expect = 5e-07, Method: Compositional matrix adjust.
Identities = 43/132 (32%), Positives = 65/132 (49%), Gaps = 8/132 (6%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACV--VFFISALM 58
DR FY DWWN++SF Y+R WN+ +H +L+ + + L +F AA + VF +SA++
Sbjct 398 DRMFYKDWWNSTSFANYYRTWNVVVHDWLYYYAYRDFLWFLGRRFKAAAMLFVFTVSAVV 457
Query 59 HEYLVVVPLQLGW-TGFIFFAFIGQIPLMYFTNIQFFQDNPTVGNCFFWLVFCFTGQPLG 117
HEY + + + FI F G M F I + + N W F GQ +
Sbjct 458 HEYALGICFGFFYPVLFILFMCFG----MLFNFILHDRRRGPIWNVIMWTSL-FLGQGVL 512
Query 118 ILLYWYLWGVKQ 129
I LY W ++
Sbjct 513 ICLYSQEWYAQR 524
> dre:564868 hypothetical LOC564868
Length=395
Score = 43.9 bits (102), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 17/38 (44%), Positives = 26/38 (68%), Gaps = 0/38 (0%)
Query 1 DREFYLDWWNASSFGEYWRKWNLPIHFFLHRHVNKPLL 38
DR FY DWWN++SF Y+R WN+ +H +L+ + + L
Sbjct 354 DRMFYKDWWNSTSFANYYRTWNIVVHDWLYYYAYRDFL 391
> xla:397828 porcn, Xporc, xporc-d; porcupine homolog; K00181
porcupine
Length=461
Score = 34.7 bits (78), Expect = 0.094, Method: Compositional matrix adjust.
Identities = 25/66 (37%), Positives = 35/66 (53%), Gaps = 5/66 (7%)
Query 13 SFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE---YLVVVPLQL 69
S E WNLP+ +LH +V K L+ G KF A V + SAL+H +L V L L
Sbjct 297 SMVEVVTSWNLPMSRWLHTYVFKNALKLG--KFHAIIVTYAASALLHGMSFHLAAVLLSL 354
Query 70 GWTGFI 75
G+ ++
Sbjct 355 GFITYV 360
> cel:T07H6.2 mom-1; More Of MS family member (mom-1); K00181
porcupine
Length=442
Score = 32.3 bits (72), Expect = 0.51, Method: Compositional matrix adjust.
Identities = 22/62 (35%), Positives = 32/62 (51%), Gaps = 8/62 (12%)
Query 20 KWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFF---ISALMHEYLVVVPLQLGWTGFIF 76
+WN P H FLH ++ K L N AC VFF +S+L+H + + L GFI
Sbjct 284 EWNKPFHTFLHENIFKRRLFNST-----ACNVFFTFAVSSLLHGLDFQMTITLLALGFIA 338
Query 77 FA 78
++
Sbjct 339 YS 340
> mmu:53627 Porcn, 2410004O13Rik, AW045557, DXHXS7465e, Mporc,
Mporc-a, Mporc-b, Mporc-c, Mporc-d, Ppn, mMg61, porc; porcupine
homolog (Drosophila); K00181 porcupine
Length=450
Score = 31.6 bits (70), Expect = 0.90, Method: Compositional matrix adjust.
Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 5/66 (7%)
Query 13 SFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE---YLVVVPLQL 69
S E WNLP+ ++L+ +V K LR G F A V + SAL+H +L V L L
Sbjct 286 SMVEVVTSWNLPMSYWLNNYVFKNALRLGT--FSAVLVTYAASALLHGFSFHLAAVLLSL 343
Query 70 GWTGFI 75
+ ++
Sbjct 344 AFITYV 349
> hsa:64840 PORCN, DHOF, FODH, MG61, MGC29687, PORC, PPN, por;
porcupine homolog (Drosophila); K00181 porcupine
Length=450
Score = 31.6 bits (70), Expect = 0.94, Method: Compositional matrix adjust.
Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 5/66 (7%)
Query 13 SFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE---YLVVVPLQL 69
S E WNLP+ ++L+ +V K LR G F A V + SAL+H +L V L L
Sbjct 286 SMVEVVTSWNLPMSYWLNNYVFKNALRLGT--FSAVLVTYAASALLHGFSFHLAAVLLSL 343
Query 70 GWTGFI 75
+ ++
Sbjct 344 AFITYV 349
> ath:AT1G34520 long-chain-alcohol O-fatty-acyltransferase family
protein / wax synthase family protein
Length=287
Score = 31.2 bits (69), Expect = 1.0, Method: Compositional matrix adjust.
Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 8/58 (13%)
Query 11 ASSFGEYW-RKWNL-------PIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE 60
A+S ++W R+WNL P + R V +P +R+ +F FF+S L+HE
Sbjct 185 ATSLQDFWGRRWNLMVPAIFRPGVYHPMRSVCQPQMRSDWARFMGCWTTFFVSGLIHE 242
> ath:AT5G55380 membrane bound O-acyl transferase (MBOAT) family
protein / wax synthase-related
Length=341
Score = 30.8 bits (68), Expect = 1.5, Method: Compositional matrix adjust.
Identities = 21/61 (34%), Positives = 30/61 (49%), Gaps = 10/61 (16%)
Query 11 ASSFGEYW-RKWNLPIHFFLHRHVNKPLLR--------NGVPKFFAACVVFFISALMHEY 61
A+S ++W R+WNL + L V+ P+ R +G F FF+S LMHE
Sbjct 192 ATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGA-MFAGVMASFFVSGLMHEL 250
Query 62 L 62
L
Sbjct 251 L 251
> pfa:MAL13P1.93 conserved Plasmodium protein, unknown function
Length=974
Score = 29.6 bits (65), Expect = 3.6, Method: Composition-based stats.
Identities = 17/70 (24%), Positives = 36/70 (51%), Gaps = 4/70 (5%)
Query 26 HFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHEYLVVVPLQLGWTGFIFFAFIGQIPL 85
+FF++ H++ L N +++C +FI+ +H Y +++ L ++F L
Sbjct 480 NFFINIHIDDKLYCN----IYSSCWFYFITTHVHIYTMILQLLHSIPVYVFPYTNNPYYL 535
Query 86 MYFTNIQFFQ 95
+ F NI F++
Sbjct 536 IDFFNISFYK 545
> dre:334395 slc43a1a, fi47a12, slc43a1, wu:fi47a12, zgc:55850;
solute carrier family 43, member 1a
Length=373
Score = 28.9 bits (63), Expect = 6.2, Method: Compositional matrix adjust.
Identities = 17/54 (31%), Positives = 26/54 (48%), Gaps = 1/54 (1%)
Query 22 NLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHEYL-VVVPLQLGWTGF 74
LP+ + R +PL G F +CV+ +SA E L ++ L L + GF
Sbjct 90 TLPLGILMDRFGPRPLRLAGSACFALSCVMMSVSAYQPEVLSAIILLALSFNGF 143
> dre:100006275 novel protein similar to vertebrate porcupine
homolog; K00181 porcupine
Length=442
Score = 28.5 bits (62), Expect = 6.4, Method: Compositional matrix adjust.
Identities = 22/66 (33%), Positives = 34/66 (51%), Gaps = 5/66 (7%)
Query 13 SFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE---YLVVVPLQL 69
S E WNLP+ +L+ +V + LR G F A + + SAL+H ++ V L L
Sbjct 286 SMVEVVTSWNLPMSRWLNTYVFRKALRYG--SFTAIIMTYTASALLHGLSFHIGAVLLTL 343
Query 70 GWTGFI 75
G+ +I
Sbjct 344 GFITYI 349
> ath:AT1G12840 DET3; DET3 (DE-ETIOLATED 3); proton-transporting
ATPase, rotational mechanism; K02148 V-type H+-transporting
ATPase subunit C [EC:3.6.3.14]
Length=375
Score = 28.5 bits (62), Expect = 6.8, Method: Compositional matrix adjust.
Identities = 15/43 (34%), Positives = 24/43 (55%), Gaps = 3/43 (6%)
Query 9 WNASSFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVV 51
W +S+GE + W +HF R + ++R G+P F ACV+
Sbjct 281 WCYTSYGEVFSSW---MHFCAVRTFAESIMRYGLPPAFLACVL 320
> dre:100148644 porcupine-like; K00181 porcupine
Length=449
Score = 28.5 bits (62), Expect = 7.9, Method: Compositional matrix adjust.
Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 5/66 (7%)
Query 13 SFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE---YLVVVPLQL 69
S E WNLP+ +L+ +V K L+ G F A V + SAL+H +L V L L
Sbjct 286 SMVEVVTSWNLPMSKWLNAYVFKSALKLGT--FPAILVTYTASALLHGLSFHLGAVLLSL 343
Query 70 GWTGFI 75
G+ ++
Sbjct 344 GFITYV 349
> dre:333999 PORCN, fi26a04, wu:fi26a04; zgc:55392; K00181 porcupine
Length=449
Score = 28.1 bits (61), Expect = 8.4, Method: Compositional matrix adjust.
Identities = 23/66 (34%), Positives = 34/66 (51%), Gaps = 5/66 (7%)
Query 13 SFGEYWRKWNLPIHFFLHRHVNKPLLRNGVPKFFAACVVFFISALMHE---YLVVVPLQL 69
S E WNLP+ +L+ +V K L+ G F A V + SAL+H +L V L L
Sbjct 286 SMVEVVTSWNLPMSKWLNAYVFKSALKLGT--FPAILVTYTASALLHGLSFHLGAVLLSL 343
Query 70 GWTGFI 75
G+ ++
Sbjct 344 GFITYV 349
Lambda K H
0.333 0.148 0.541
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2749206264
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40