bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_4079_orf1
Length=85
Score E
Sequences producing significant alignments: (Bits) Value
pfa:PF14_0316 DNA topoisomerase II, putative (EC:3.6.1.-); K03... 108 5e-24
cpv:cgd4_780 DNA topoisomerase II ; K03164 DNA topoisomerase I... 104 6e-23
tgo:TGME49_112230 DNA topoisomerase II, putative ; K03164 DNA ... 102 3e-22
mmu:21974 Top2b, D230016L12Rik, Top-2; topoisomerase (DNA) II ... 85.5 4e-17
hsa:7155 TOP2B, TOPIIB, top2beta; topoisomerase (DNA) II beta ... 85.5 4e-17
dre:569877 top2b, im:7138121, si:dkey-98n4.1, wu:fc38e02; topo... 84.7 7e-17
ath:AT3G23890 TOPII; TOPII (TOPOISOMERASE II); ATP binding / D... 82.8 3e-16
xla:398512 top2a, top2, tp2a; topoisomerase (DNA) II alpha 170... 82.4 3e-16
mmu:21973 Top2a, Top-2; topoisomerase (DNA) II alpha (EC:5.99.... 82.0 5e-16
hsa:7153 TOP2A, TOP2, TP2A; topoisomerase (DNA) II alpha 170kD... 81.6 6e-16
tpv:TP02_0467 DNA topoisomerase II; K03164 DNA topoisomerase I... 80.9 1e-15
dre:323733 top2a, MGC92574, fc08c07, wu:fc08c07; topoisomerase... 79.0 4e-15
cel:K12D12.1 top-2; TOPoisomerase family member (top-2); K0316... 74.7 6e-14
bbo:BBOV_III004820 17.m07431; DNA topoisomerase II; K03164 DNA... 64.7 7e-11
sce:YNL088W TOP2, TOR3, TRF3; Essential type II topoisomerase,... 62.0 5e-10
cel:R05D3.1 hypothetical protein; K03164 DNA topoisomerase II ... 54.3 1e-07
bbo:BBOV_II003880 18.m09973; hypothetical protein 27.7 8.9
> pfa:PF14_0316 DNA topoisomerase II, putative (EC:3.6.1.-); K03164
DNA topoisomerase II [EC:5.99.1.3]
Length=1472
Score = 108 bits (270), Expect = 5e-24, Method: Composition-based stats.
Identities = 49/68 (72%), Positives = 55/68 (80%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
+R HLWVFV CLI NP FDSQTKETLTT +FGSKC LS++TIN V KSPIL +LLWA
Sbjct 391 IRNHLWVFVNCLIVNPTFDSQTKETLTTKPVKFGSKCILSDKTINNVLKSPILSNILLWA 450
Query 61 QTKAQVQL 68
Q KAQV+L
Sbjct 451 QAKAQVEL 458
> cpv:cgd4_780 DNA topoisomerase II ; K03164 DNA topoisomerase
II [EC:5.99.1.3]
Length=1797
Score = 104 bits (260), Expect = 6e-23, Method: Composition-based stats.
Identities = 44/68 (64%), Positives = 57/68 (83%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ H+W+F+KCLI NP+FDSQTKETLTT VS+FGS C LSE+TIN+V KS I+E VL+WA
Sbjct 477 VKNHIWIFIKCLIVNPSFDSQTKETLTTKVSKFGSVCTLSEKTINSVVKSSIVENVLMWA 536
Query 61 QTKAQVQL 68
Q K ++L
Sbjct 537 QLKQSMEL 544
> tgo:TGME49_112230 DNA topoisomerase II, putative ; K03164 DNA
topoisomerase II [EC:5.99.1.3]
Length=1687
Score = 102 bits (254), Expect = 3e-22, Method: Composition-based stats.
Identities = 44/65 (67%), Positives = 54/65 (83%), Gaps = 0/65 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++QHLW+FV CLI NPAFDSQTKETLTT ++FGSKC L+++TI V KS I+E V+LWA
Sbjct 420 VKQHLWIFVSCLIVNPAFDSQTKETLTTKPAKFGSKCTLADKTIGQVVKSSIVENVVLWA 479
Query 61 QTKAQ 65
QTK Q
Sbjct 480 QTKQQ 484
> mmu:21974 Top2b, D230016L12Rik, Top-2; topoisomerase (DNA) II
beta (EC:5.99.1.3); K03164 DNA topoisomerase II [EC:5.99.1.3]
Length=1612
Score = 85.5 bits (210), Expect = 4e-17, Method: Composition-based stats.
Identities = 36/68 (52%), Positives = 46/68 (67%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ H+WVF+ CLIENP FDSQTKE +T FGSKC LSE+ A + I+E +L W
Sbjct 365 VKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWV 424
Query 61 QTKAQVQL 68
+ KAQ QL
Sbjct 425 KFKAQTQL 432
> hsa:7155 TOP2B, TOPIIB, top2beta; topoisomerase (DNA) II beta
180kDa (EC:5.99.1.3); K03164 DNA topoisomerase II [EC:5.99.1.3]
Length=1621
Score = 85.5 bits (210), Expect = 4e-17, Method: Composition-based stats.
Identities = 36/68 (52%), Positives = 46/68 (67%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ H+WVF+ CLIENP FDSQTKE +T FGSKC LSE+ A + I+E +L W
Sbjct 372 VKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWV 431
Query 61 QTKAQVQL 68
+ KAQ QL
Sbjct 432 KFKAQTQL 439
> dre:569877 top2b, im:7138121, si:dkey-98n4.1, wu:fc38e02; topoisomerase
(DNA) II beta (EC:5.99.1.3); K03164 DNA topoisomerase
II [EC:5.99.1.3]
Length=1618
Score = 84.7 bits (208), Expect = 7e-17, Method: Composition-based stats.
Identities = 38/68 (55%), Positives = 45/68 (66%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ HLWVFV LIENP FDSQTKE +T FGSKC LSE+ I A I+E +L W
Sbjct 376 VKNHLWVFVNALIENPTFDSQTKENMTLQTKSFGSKCALSEKFIRAATNCGIVESILNWV 435
Query 61 QTKAQVQL 68
+ KAQ QL
Sbjct 436 KFKAQTQL 443
> ath:AT3G23890 TOPII; TOPII (TOPOISOMERASE II); ATP binding /
DNA binding / DNA topoisomerase (ATP-hydrolyzing)/ DNA topoisomerase/
DNA-dependent ATPase; K03164 DNA topoisomerase II
[EC:5.99.1.3]
Length=1438
Score = 82.8 bits (203), Expect = 3e-16, Method: Composition-based stats.
Identities = 39/68 (57%), Positives = 46/68 (67%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ HLWVFV LI+NPAFDSQTKETLT S FGSKC LSE + V KS ++E +L WA
Sbjct 358 VKNHLWVFVNALIDNPAFDSQTKETLTLRQSSFGSKCELSEDFLKKVGKSGVVENLLSWA 417
Query 61 QTKAQVQL 68
K L
Sbjct 418 DFKQNKDL 425
> xla:398512 top2a, top2, tp2a; topoisomerase (DNA) II alpha 170kDa;
K03164 DNA topoisomerase II [EC:5.99.1.3]
Length=1579
Score = 82.4 bits (202), Expect = 3e-16, Method: Composition-based stats.
Identities = 36/68 (52%), Positives = 44/68 (64%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ H+WVFV LIENP FDSQTKE +T FGSKC LSE+ I I+E +L W
Sbjct 354 VKNHMWVFVNALIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATSCGIVESILNWV 413
Query 61 QTKAQVQL 68
+ KAQ QL
Sbjct 414 KFKAQTQL 421
> mmu:21973 Top2a, Top-2; topoisomerase (DNA) II alpha (EC:5.99.1.3);
K03164 DNA topoisomerase II [EC:5.99.1.3]
Length=1528
Score = 82.0 bits (201), Expect = 5e-16, Method: Composition-based stats.
Identities = 35/68 (51%), Positives = 45/68 (66%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ H+W+FV LIENP FDSQTKE +T FGS C LSE+ I A I+E +L W
Sbjct 355 VKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWV 414
Query 61 QTKAQVQL 68
+ KAQ+QL
Sbjct 415 KFKAQIQL 422
> hsa:7153 TOP2A, TOP2, TP2A; topoisomerase (DNA) II alpha 170kDa
(EC:5.99.1.3); K03164 DNA topoisomerase II [EC:5.99.1.3]
Length=1531
Score = 81.6 bits (200), Expect = 6e-16, Method: Composition-based stats.
Identities = 36/68 (52%), Positives = 45/68 (66%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ H+W+FV LIENP FDSQTKE +T FGS C LSE+ I A I+E +L W
Sbjct 356 VKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWV 415
Query 61 QTKAQVQL 68
+ KAQVQL
Sbjct 416 KFKAQVQL 423
> tpv:TP02_0467 DNA topoisomerase II; K03164 DNA topoisomerase
II [EC:5.99.1.3]
Length=1378
Score = 80.9 bits (198), Expect = 1e-15, Method: Composition-based stats.
Identities = 33/68 (48%), Positives = 49/68 (72%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ H++VF+ C I NP+FDSQTKETLTT + +FGSK +S++T+N V KS I + +L W
Sbjct 335 IKNHIFVFINCQIVNPSFDSQTKETLTTKIVKFGSKYTMSDKTLNQVLKSKIFDNILSWL 394
Query 61 QTKAQVQL 68
K ++L
Sbjct 395 HEKTNIEL 402
> dre:323733 top2a, MGC92574, fc08c07, wu:fc08c07; topoisomerase
(DNA) II alpha (EC:5.99.1.3); K03164 DNA topoisomerase II
[EC:5.99.1.3]
Length=1574
Score = 79.0 bits (193), Expect = 4e-15, Method: Composition-based stats.
Identities = 32/68 (47%), Positives = 46/68 (67%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ H+W+FV CLIENP+FDSQTKE +T FGS C+LS++ + I+E ++ W
Sbjct 355 VKNHMWLFVNCLIENPSFDSQTKENMTLQQKNFGSACSLSDKFVKQANGCGIVESIMNWV 414
Query 61 QTKAQVQL 68
+ KAQ QL
Sbjct 415 KFKAQAQL 422
> cel:K12D12.1 top-2; TOPoisomerase family member (top-2); K03164
DNA topoisomerase II [EC:5.99.1.3]
Length=1520
Score = 74.7 bits (182), Expect = 6e-14, Method: Composition-based stats.
Identities = 32/63 (50%), Positives = 42/63 (66%), Gaps = 0/63 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ H+WVFV CLIENP FDSQTKET+T +FGS C LSE+ A + I + V+ W
Sbjct 391 IKNHMWVFVNCLIENPTFDSQTKETMTLQQKQFGSTCVLSEKFSKAASSVGITDAVMSWV 450
Query 61 QTK 63
+ K
Sbjct 451 RFK 453
> bbo:BBOV_III004820 17.m07431; DNA topoisomerase II; K03164 DNA
topoisomerase II [EC:5.99.1.3]
Length=1437
Score = 64.7 bits (156), Expect = 7e-11, Method: Composition-based stats.
Identities = 28/68 (41%), Positives = 47/68 (69%), Gaps = 0/68 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPILEGVLLWA 60
++ ++++FV C I NP FDSQTKETLTT ++FGSK ++ + + + KS + E +L +A
Sbjct 336 IKNYIFLFVNCQIVNPTFDSQTKETLTTKPAKFGSKYIMNPKAVAQILKSTLTERILSFA 395
Query 61 QTKAQVQL 68
Q + ++L
Sbjct 396 QGRLNIEL 403
> sce:YNL088W TOP2, TOR3, TRF3; Essential type II topoisomerase,
relieves torsional strain in DNA by cleaving and re-sealing
the phosphodiester backbone of both positively and negatively
supercoiled DNA; cleaves complementary strands; localizes
to axial cores in meiosis (EC:5.99.1.3); K03164 DNA topoisomerase
II [EC:5.99.1.3]
Length=1428
Score = 62.0 bits (149), Expect = 5e-10, Method: Composition-based stats.
Identities = 25/52 (48%), Positives = 37/52 (71%), Gaps = 0/52 (0%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGSKCNLSERTINAVAKSPI 52
++ ++++F+ CLIENPAF SQTKE LTT V FGS+C + IN + K+ +
Sbjct 345 IKNNMFIFINCLIENPAFTSQTKEQLTTRVKDFGSRCEIPLEYINKIMKTDL 396
> cel:R05D3.1 hypothetical protein; K03164 DNA topoisomerase II
[EC:5.99.1.3]
Length=1054
Score = 54.3 bits (129), Expect = 1e-07, Method: Composition-based stats.
Identities = 25/59 (42%), Positives = 42/59 (71%), Gaps = 3/59 (5%)
Query 1 LRQHLWVFVKCLIENPAFDSQTKETLTTTVSRFGS--KCNLSERTINAVAKSPILEGVL 57
++ +L +F+ CLIENP+F+SQTKETLTT FGS +C+ +++T +S ++E ++
Sbjct 260 IKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECD-AKKTAEWAEQSGLIEDIV 317
> bbo:BBOV_II003880 18.m09973; hypothetical protein
Length=562
Score = 27.7 bits (60), Expect = 8.9, Method: Composition-based stats.
Identities = 18/41 (43%), Positives = 21/41 (51%), Gaps = 3/41 (7%)
Query 22 TKETLTTTVSRFGSKCNLSERTINAVAKSP---ILEGVLLW 59
TKE TVSRFG L E T N +S +L+ V LW
Sbjct 241 TKEIQLGTVSRFGRILQLIEATFNVCKRSRSDLVLDLVPLW 281
Lambda K H
0.325 0.133 0.418
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2035463248
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40