bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_3355_orf2
Length=180
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_033720 RNA polymerase, putative ; K03016 DNA-direct... 194 1e-49
cpv:cgd1_2260 RNA polymerase II B8 subunit ; K03016 DNA-direct... 169 4e-42
tpv:TP01_0233 RNA polymerase subunit; K03016 DNA-directed RNA ... 166 4e-41
bbo:BBOV_IV005100 23.m06188; RNA polymerase subunit 8c; K03016... 164 2e-40
pfa:PFL0665c RNA polymerase subunit 8c, putative (EC:2.7.7.6);... 155 5e-38
dre:792188 RNA polymerase II, polypeptide H-like; K03016 DNA-d... 92.0 8e-19
dre:550263 zgc:110289; K03016 DNA-directed RNA polymerases I, ... 92.0 8e-19
sce:YOR224C RPB8; ABC14.5; K03016 DNA-directed RNA polymerases... 88.2 1e-17
mmu:245841 Polr2h, MGC103049, MGC8248; polymerase (RNA) II (DN... 87.4 3e-17
hsa:5437 POLR2H, RPABC3, RPB17, RPB8, hsRPB8; polymerase (RNA)... 87.4 3e-17
xla:443896 polr2h, MGC80152; polymerase (RNA) II (DNA directed... 85.5 9e-17
cel:F26F4.11 rpb-8; RNA Polymerase II (B) subunit family membe... 82.0 1e-15
ath:AT1G54250 NRPB8A; NRPB8A; DNA-directed RNA polymerase; K03... 80.9 2e-15
ath:AT3G59600 NRPB8B; NRPB8B; DNA-directed RNA polymerase; K03... 80.5 3e-15
eco:b4056 yjbQ, ECK4048, JW4017; thiamin phosphate synthase 32.0 1.1
xla:398196 mcm10, MGC84836, xmcm10; minichromosome maintenance... 29.3 7.9
> tgo:TGME49_033720 RNA polymerase, putative ; K03016 DNA-directed
RNA polymerases I, II, and III subunit RPABC3
Length=149
Score = 194 bits (494), Expect = 1e-49, Method: Compositional matrix adjust.
Identities = 98/143 (68%), Positives = 114/143 (79%), Gaps = 4/143 (2%)
Query 38 MSIPCLFEDRFTVRNVDNGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLT 97
MSIPCLFEDRF VR+VD KFERV+R++ KSSG DADL LDV++ L PV EKQ L+IG+T
Sbjct 1 MSIPCLFEDRFEVRSVDCSKFERVARLKGKSSGFDADLQLDVNSDLFPVKEKQRLYIGIT 60
Query 98 NSL--SGPDRKGDGAAD--SQVLSGYDYVMYGKVFKLEETSSERRTLYLSFGGLLMSLAA 153
N+L SG RK D A + L YDYVMYGKVF+ EE SSERRTLY SFGGLLM+L A
Sbjct 61 NNLHSSGDARKDDSAWTEPAAYLKEYDYVMYGKVFRTEECSSERRTLYASFGGLLMALTA 120
Query 154 DKHVVGDLELDMRVYLLVKRSPE 176
DKHVVGDLELDMR+Y+L++RS E
Sbjct 121 DKHVVGDLELDMRIYILIRRSDE 143
> cpv:cgd1_2260 RNA polymerase II B8 subunit ; K03016 DNA-directed
RNA polymerases I, II, and III subunit RPABC3
Length=144
Score = 169 bits (429), Expect = 4e-42, Method: Compositional matrix adjust.
Identities = 80/144 (55%), Positives = 111/144 (77%), Gaps = 3/144 (2%)
Query 38 MSIP-CLFEDRFTVRNVDNGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGL 96
MS+P CLFEDRF + +VD+GKF+R+ RIR +S G DADLILD++N+L PV EK+ L+IGL
Sbjct 1 MSLPSCLFEDRFVINSVDSGKFDRIGRIRGRSVGFDADLILDINNELFPVREKESLYIGL 60
Query 97 TN--SLSGPDRKGDGAADSQVLSGYDYVMYGKVFKLEETSSERRTLYLSFGGLLMSLAAD 154
++ S++ D D + ++ YDYVMYGK++++EE S+RR+LY SFGGLLMSL AD
Sbjct 61 SSQVSVNPNDIVWDNSGPQTLMDQYDYVMYGKIYRVEEKQSDRRSLYASFGGLLMSLTAD 120
Query 155 KHVVGDLELDMRVYLLVKRSPEAL 178
K++VGD LDMR+Y LV+RS + +
Sbjct 121 KNLVGDFSLDMRLYCLVRRSEDII 144
> tpv:TP01_0233 RNA polymerase subunit; K03016 DNA-directed RNA
polymerases I, II, and III subunit RPABC3
Length=145
Score = 166 bits (420), Expect = 4e-41, Method: Compositional matrix adjust.
Identities = 84/135 (62%), Positives = 102/135 (75%), Gaps = 3/135 (2%)
Query 42 CLFEDRFTVRNVDNGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLTNSL- 100
C+FEDRF VR++DN KFERVSR+ AKS+G DA+L+LD ++ LL V KQ LHI +TNSL
Sbjct 6 CVFEDRFLVRSIDNSKFERVSRLSAKSTGFDAELLLDFNSDLLQVYNKQVLHILITNSLL 65
Query 101 -SGPDRKGDGAADSQVLSG-YDYVMYGKVFKLEETSSERRTLYLSFGGLLMSLAADKHVV 158
SG D + D L G Y+Y MYGK+FK EE SSE RT+Y SFGGLLMSL ADK VV
Sbjct 66 PSGSDVNLSESVDIPSLLGDYEYAMYGKIFKFEEVSSETRTIYASFGGLLMSLTADKQVV 125
Query 159 GDLELDMRVYLLVKR 173
DL+LDM++YLLV+R
Sbjct 126 ADLDLDMKIYLLVRR 140
> bbo:BBOV_IV005100 23.m06188; RNA polymerase subunit 8c; K03016
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Length=145
Score = 164 bits (415), Expect = 2e-40, Method: Compositional matrix adjust.
Identities = 78/138 (56%), Positives = 102/138 (73%), Gaps = 3/138 (2%)
Query 39 SIPCLFEDRFTVRNVDNGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLTN 98
S C+FEDRF VR++DN KFERVSRI AKS+G DA+L+LD+++ + PV K LHI +TN
Sbjct 3 SATCVFEDRFVVRSIDNSKFERVSRITAKSTGFDAELLLDINSDIFPVTVKSMLHILMTN 62
Query 99 SL--SGPDRKGDGAAD-SQVLSGYDYVMYGKVFKLEETSSERRTLYLSFGGLLMSLAADK 155
+L SG D +D ++ Y+Y MYGK+FK E+ SSE RT+Y SFGGLLMSL ADK
Sbjct 63 NLLPSGSDVNLSDYSDLPSLMRDYEYAMYGKIFKFEDVSSETRTIYASFGGLLMSLTADK 122
Query 156 HVVGDLELDMRVYLLVKR 173
V+ DL+LDMR+YL+ +R
Sbjct 123 QVIADLDLDMRIYLMARR 140
> pfa:PFL0665c RNA polymerase subunit 8c, putative (EC:2.7.7.6);
K03016 DNA-directed RNA polymerases I, II, and III subunit
RPABC3
Length=145
Score = 155 bits (393), Expect = 5e-38, Method: Compositional matrix adjust.
Identities = 72/136 (52%), Positives = 101/136 (74%), Gaps = 1/136 (0%)
Query 38 MSIPCLFEDRFTVRNVDNGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLT 97
M+ LFEDRF + +VDN KFE+VSRI+AKS+G DA+LILDVH++L V EK+ +++ L
Sbjct 1 MASNILFEDRFVISSVDNSKFEKVSRIKAKSTGYDAELILDVHSELFKVEEKKAIYLALQ 60
Query 98 NSL-SGPDRKGDGAADSQVLSGYDYVMYGKVFKLEETSSERRTLYLSFGGLLMSLAADKH 156
+ L + D KG ++ L+ +Y+M G++FK EE SSERRT+Y SFGGL+M+L DK
Sbjct 61 DKLLNRNDEKGWEQNENVPLNNIEYIMSGRIFKFEELSSERRTVYASFGGLMMALTTDKQ 120
Query 157 VVGDLELDMRVYLLVK 172
+GDLE+DM++YLL K
Sbjct 121 FIGDLEIDMKIYLLTK 136
> dre:792188 RNA polymerase II, polypeptide H-like; K03016 DNA-directed
RNA polymerases I, II, and III subunit RPABC3
Length=150
Score = 92.0 bits (227), Expect = 8e-19, Method: Compositional matrix adjust.
Identities = 58/143 (40%), Positives = 84/143 (58%), Gaps = 12/143 (8%)
Query 43 LFEDRFTVRNVD--NGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLTNSL 100
LFED F V+++D KF+RVSR+ +S DLILDV+ Q+ PV + + ++L
Sbjct 5 LFEDIFDVKDIDPDGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTL 64
Query 101 SGPDRKGDGAADSQV-----LSGYDYVMYGKVFKLE--ETSSE---RRTLYLSFGGLLMS 150
DG + Q +DYVMYGKV+++E ETS+E R + Y+S+GGLLM
Sbjct 65 YEDGTPDDGEYNPQDDRPSRADQFDYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMR 124
Query 151 LAADKHVVGDLELDMRVYLLVKR 173
L D + + E+D RVYLL+K+
Sbjct 125 LQGDANNLHGFEVDSRVYLLMKK 147
> dre:550263 zgc:110289; K03016 DNA-directed RNA polymerases I,
II, and III subunit RPABC3
Length=150
Score = 92.0 bits (227), Expect = 8e-19, Method: Compositional matrix adjust.
Identities = 58/143 (40%), Positives = 84/143 (58%), Gaps = 12/143 (8%)
Query 43 LFEDRFTVRNVD--NGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLTNSL 100
LFED F V+++D KF+RVSR+ +S DLILDV+ Q+ PV + + ++L
Sbjct 5 LFEDIFDVKDIDPDGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTL 64
Query 101 SGPDRKGDGAADSQV-----LSGYDYVMYGKVFKLE--ETSSE---RRTLYLSFGGLLMS 150
DG + Q +DYVMYGKV+++E ETS+E R + Y+S+GGLLM
Sbjct 65 YEDGTPDDGEYNPQDDRPSRADQFDYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMR 124
Query 151 LAADKHVVGDLELDMRVYLLVKR 173
L D + + E+D RVYLL+K+
Sbjct 125 LQGDANNLHGFEVDSRVYLLMKK 147
> sce:YOR224C RPB8; ABC14.5; K03016 DNA-directed RNA polymerases
I, II, and III subunit RPABC3
Length=146
Score = 88.2 bits (217), Expect = 1e-17, Method: Compositional matrix adjust.
Identities = 52/143 (36%), Positives = 77/143 (53%), Gaps = 13/143 (9%)
Query 43 LFEDRFTVRNVDNGKFERVSRIRAKSSGLD-ADLILDVHNQLLPVAEKQGLHIGLTNSLS 101
LF+D F V VD G++ +V RI A S+ D L LD++ +L PVA + L + + +SL+
Sbjct 5 LFDDIFQVSEVDPGRYNKVCRIEAASTTQDQCKLTLDINVELFPVAAQDSLTVTIASSLN 64
Query 102 GPDRKGDG-----------AADSQVLSGYDYVMYGKVFKLEETSSERRTLYLSFGGLLMS 150
D + A D + YDYVMYG +K EE S + +Y SFGGLLM
Sbjct 65 LEDTPANDSSATRSWRPPQAGDRSLADDYDYVMYGTAYKFEEVSKDLIAVYYSFGGLLMR 124
Query 151 LAADKHVVGDLELDMRVYLLVKR 173
L + + +L+ + YLL++R
Sbjct 125 LEGNYRNLNNLKQE-NAYLLIRR 146
> mmu:245841 Polr2h, MGC103049, MGC8248; polymerase (RNA) II (DNA
directed) polypeptide H (EC:2.7.7.6); K03016 DNA-directed
RNA polymerases I, II, and III subunit RPABC3
Length=150
Score = 87.4 bits (215), Expect = 3e-17, Method: Compositional matrix adjust.
Identities = 56/143 (39%), Positives = 83/143 (58%), Gaps = 12/143 (8%)
Query 43 LFEDRFTVRNVD--NGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLTNSL 100
LFED F V+++D KF+RVSR+ +S DLILDV+ Q+ PV + + ++L
Sbjct 5 LFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTL 64
Query 101 SGPDRKGDGAADS-----QVLSGYDYVMYGKVFKLE--ETSSE---RRTLYLSFGGLLMS 150
DG + ++YVMYGKV+++E ETS+E R + Y+S+GGLLM
Sbjct 65 YEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMR 124
Query 151 LAADKHVVGDLELDMRVYLLVKR 173
L D + + E+D RVYLL+K+
Sbjct 125 LQGDANNLHGFEVDSRVYLLMKK 147
> hsa:5437 POLR2H, RPABC3, RPB17, RPB8, hsRPB8; polymerase (RNA)
II (DNA directed) polypeptide H (EC:2.7.7.6); K03016 DNA-directed
RNA polymerases I, II, and III subunit RPABC3
Length=150
Score = 87.4 bits (215), Expect = 3e-17, Method: Compositional matrix adjust.
Identities = 56/143 (39%), Positives = 83/143 (58%), Gaps = 12/143 (8%)
Query 43 LFEDRFTVRNVD--NGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLTNSL 100
LFED F V+++D KF+RVSR+ +S DLILDV+ Q+ PV + + ++L
Sbjct 5 LFEDIFDVKDIDPEGKKFDRVSRLHCESESFKMDLILDVNIQIYPVDLGDKFRLVIASTL 64
Query 101 SGPDRKGDGAADS-----QVLSGYDYVMYGKVFKLE--ETSSE---RRTLYLSFGGLLMS 150
DG + ++YVMYGKV+++E ETS+E R + Y+S+GGLLM
Sbjct 65 YEDGTLDDGEYNPTDDRPSRADQFEYVMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMR 124
Query 151 LAADKHVVGDLELDMRVYLLVKR 173
L D + + E+D RVYLL+K+
Sbjct 125 LQGDANNLHGFEVDSRVYLLMKK 147
> xla:443896 polr2h, MGC80152; polymerase (RNA) II (DNA directed)
polypeptide H; K03016 DNA-directed RNA polymerases I, II,
and III subunit RPABC3
Length=150
Score = 85.5 bits (210), Expect = 9e-17, Method: Compositional matrix adjust.
Identities = 55/143 (38%), Positives = 83/143 (58%), Gaps = 12/143 (8%)
Query 43 LFEDRFTVRNVD--NGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLTNSL 100
LFED F V+++D KF+RVSR+ +S DLILDV+ Q+ PV + + ++L
Sbjct 5 LFEDIFDVKDIDPEGRKFDRVSRLHCESESFKMDLILDVNIQVYPVDLGDKFRLVVASTL 64
Query 101 SGPDRKGDGAADS-----QVLSGYDYVMYGKVFKLE--ETSSE---RRTLYLSFGGLLMS 150
DG + ++Y+MYGKV+++E ETS+E R + Y+S+GGLLM
Sbjct 65 YEDGTLDDGEYNPTDDRPSRADQFEYIMYGKVYRIEGDETSTEAATRLSAYVSYGGLLMR 124
Query 151 LAADKHVVGDLELDMRVYLLVKR 173
L D + + E+D RVYLL+K+
Sbjct 125 LQGDANNLHGFEVDSRVYLLMKK 147
> cel:F26F4.11 rpb-8; RNA Polymerase II (B) subunit family member
(rpb-8); K03016 DNA-directed RNA polymerases I, II, and
III subunit RPABC3
Length=148
Score = 82.0 bits (201), Expect = 1e-15, Method: Compositional matrix adjust.
Identities = 48/142 (33%), Positives = 80/142 (56%), Gaps = 10/142 (7%)
Query 43 LFEDRFTVRNVD--NGKFERVSRIRAKSSGLDADLILDVHNQLLPVAEKQGLHIGLTNSL 100
+F+D F V++VD KF+RVSR + +LI+D+++Q+ P+ + + + L +L
Sbjct 5 IFDDMFKVKSVDPDGKKFDRVSRYFCDAESFKMELIIDINSQIYPLKQNDKVRLVLATTL 64
Query 101 SGPDRKGDGAADSQV----LSGYDYVMYGKVFKLEE----TSSERRTLYLSFGGLLMSLA 152
+G D + + Y+YVMYGKV++LE+ T + Y SFGGLLM L
Sbjct 65 REDGLADEGEYDPKAEYPRIKQYEYVMYGKVYRLEDDDTGTDGGKLAAYASFGGLLMRLK 124
Query 153 ADKHVVGDLELDMRVYLLVKRS 174
+ + E+DM +YLL+K++
Sbjct 125 GEAINLHGFEVDMNLYLLMKKT 146
> ath:AT1G54250 NRPB8A; NRPB8A; DNA-directed RNA polymerase; K03016
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Length=146
Score = 80.9 bits (198), Expect = 2e-15, Method: Compositional matrix adjust.
Identities = 50/140 (35%), Positives = 81/140 (57%), Gaps = 8/140 (5%)
Query 42 CLFEDRFTVRNVD--NGKFERVSRIRAKSSGLDADLILDVHNQLLPVA--EKQGLHIGLT 97
LFED F V +D KF++V+R++A S L+ + LDV+ ++ P+A +K L + T
Sbjct 6 ILFEDIFVVDQLDPDGKKFDKVTRVQATSHNLEMFMHLDVNTEVYPLAVGDKFTLALAPT 65
Query 98 NSLSGPDRKG--DGAADSQVLSGYDYVMYGKVFKLEET--SSERRTLYLSFGGLLMSLAA 153
+L G G A + Y+Y+M+GK++K+ E + + LY+SFGGLLM L
Sbjct 66 LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLKG 125
Query 154 DKHVVGDLELDMRVYLLVKR 173
D + ELD R++LL+++
Sbjct 126 DPAHISHFELDQRLFLLMRK 145
> ath:AT3G59600 NRPB8B; NRPB8B; DNA-directed RNA polymerase; K03016
DNA-directed RNA polymerases I, II, and III subunit RPABC3
Length=146
Score = 80.5 bits (197), Expect = 3e-15, Method: Compositional matrix adjust.
Identities = 49/140 (35%), Positives = 81/140 (57%), Gaps = 8/140 (5%)
Query 42 CLFEDRFTVRNVD--NGKFERVSRIRAKSSGLDADLILDVHNQLLPVA--EKQGLHIGLT 97
+FED F V +D KF++V+R+ A+S L+ + LDV+ ++ P+A +K L + T
Sbjct 6 IMFEDIFVVDKLDPDGKKFDKVTRVEARSHNLEMFMHLDVNTEVYPLAVGDKFTLAMAPT 65
Query 98 NSLSGPDRKG--DGAADSQVLSGYDYVMYGKVFKLEET--SSERRTLYLSFGGLLMSLAA 153
+L G G A + Y+Y+M+GK++K+ E + + LY+SFGGLLM L
Sbjct 66 LNLDGTPDTGYFTPGAKKTLADKYEYIMHGKLYKISERDGKTPKAELYVSFGGLLMLLQG 125
Query 154 DKHVVGDLELDMRVYLLVKR 173
D + ELD R++LL+++
Sbjct 126 DPAHISHFELDQRLFLLMRK 145
> eco:b4056 yjbQ, ECK4048, JW4017; thiamin phosphate synthase
Length=138
Score = 32.0 bits (71), Expect = 1.1, Method: Compositional matrix adjust.
Identities = 28/83 (33%), Positives = 39/83 (46%), Gaps = 16/83 (19%)
Query 27 LLLQQVAAAATMSIPCLFE-----DRFTVRNV-DNGKFER--------VSRIRAKSSGLD 72
LLLQ +A+ T++ C +RF +R V DNG +E S I KSS L
Sbjct 40 LLLQHTSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHI--KSSMLG 97
Query 73 ADLILDVHNQLLPVAEKQGLHIG 95
L+L VH + QG+ +G
Sbjct 98 TSLVLPVHKGRIQTGTWQGIWLG 120
> xla:398196 mcm10, MGC84836, xmcm10; minichromosome maintenance
complex component 10; K10736 minichromosome maintenance protein
10
Length=860
Score = 29.3 bits (64), Expect = 7.9, Method: Composition-based stats.
Identities = 19/82 (23%), Positives = 46/82 (56%), Gaps = 8/82 (9%)
Query 19 RRGSSSKLLLLQQVAAAATMSI-------PCLFEDRFTVRNVDNGKFERVSRIRAKSSGL 71
+RGSSS+ L+ Q+VA+ A+ S P + + R + +++ +F+++ ++K +G+
Sbjct 670 KRGSSSEELVAQRVASHASTSPKSPDENEPAIKKHRDQLAYLESEEFQKILNAKSKHTGI 729
Query 72 DADLILDVHNQLL-PVAEKQGL 92
+ +++ P+ +K+ L
Sbjct 730 LKEAEVEIQEHYFDPLVKKEQL 751
Lambda K H
0.319 0.136 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 4859948100
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40