bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
164,496 sequences; 82,071,388 total letters
Query= Eten_1446_orf1
Length=372
Score E
Sequences producing significant alignments: (Bits) Value
cpv:cgd8_5280 Deg1p-like type II pseudouridylate synthase TruA 163 9e-40
tgo:TGME49_020220 hypothetical protein 152 2e-36
mmu:67049 Pus3, 2610020J05Rik, 5730412F04Rik; pseudouridine sy... 143 1e-33
sce:YFL001W DEG1, HRM3, PUS3; Deg1p (EC:4.2.1.70); K01855 [EC... 143 1e-33
hsa:83480 PUS3, 2610020J05Rik, FLJ23638; pseudouridylate synth... 142 1e-33
ath:AT1G34150 tRNA pseudouridine synthase family protein 137 5e-32
bbo:BBOV_III005490 17.m07491; tRNA pseudouridine synthase A fa... 134 5e-31
xla:100101318 pus3; pseudouridylate synthase 3; K01855 [EC:5.... 132 2e-30
cel:E02H1.3 tag-124; Temporarily Assigned Gene name family mem... 132 3e-30
dre:337487 pus3, fj78b12, wu:fj78b12, zgc:56653; pseudouridyla... 121 6e-27
pfa:PFI0420c tRNA pseudouridine synthase, putative (EC:4.2.1.7... 96.7 1e-19
dre:494060 pus1, wu:fb98h11, zgc:103516; pseudouridine synthas... 83.6 1e-15
hsa:80324 PUS1, MGC11268, MLASA1; pseudouridylate synthase 1 (... 79.7 1e-14
ath:AT3G06950 tRNA pseudouridine synthase family protein; K061... 75.9 2e-13
eco:b2318 truA, asuC, ECK2312, hisT, JW2315, leuK; pseudouridy... 71.6 4e-12
mmu:56361 Pus1, A730013B20Rik, MPUS1, mPus1p; pseudouridine sy... 65.9 2e-10
ath:AT2G30320 tRNA pseudouridine synthase family protein 65.5 3e-10
cel:W06H3.2 pus-1; PseudoUridine Synthase family member (pus-1... 63.5 1e-09
hsa:126789 PUSL1, FLJ90811; pseudouridylate synthase-like 1 60.8
sce:YPL212C PUS1; Pus1p (EC:4.2.1.70); K06173 tRNA pseudouridi... 59.7 2e-08
mmu:433813 Pusl1, 2810021I11Rik, AI853843, D18346; pseudouridy... 57.8 7e-08
tpv:TP01_1102 tRNA pseudouridine synthase; K06173 tRNA pseudou... 57.4 9e-08
bbo:BBOV_IV012080 23.m06054; tRNA pseudouridine synthase (EC:4... 56.6 1e-07
xla:443955 pusl1, MGC80334; pseudouridylate synthase-like 1 (E... 56.2 2e-07
xla:496371 pus1; pseudouridylate synthase 1; K06173 tRNA pseud... 51.6 4e-06
cpv:cgd8_510 Pus1p-like type II pseudousynthas TruA ; K06173 t... 47.4 8e-05
dre:555828 MGC80334 protein-like 47.4 1e-04
sce:YGL063W PUS2; Mitochondrial tRNA:pseudouridine synthase; a... 47.0 1e-04
ath:AT1G20370 tRNA pseudouridine synthase family protein; K061... 47.0 1e-04
ath:AT1G76120 tRNA pseudouridine synthase family protein; K061... 46.6 1e-04
bbo:BBOV_II003150 18.m06263; RNA pseudouridine synthase A 2 45.4
pfa:PF08_0123 pseudouridylate synthase, putative (EC:4.2.1.70)... 42.7 0.002
pfa:PFE0815w tRNA pseudouridine synthase, putative 42.0 0.004
tgo:TGME49_024200 hypothetical protein ; K06173 tRNA pseudouri... 40.8 0.007
tgo:TGME49_011450 hypothetical protein 32.3 3.0
> cpv:cgd8_5280 Deg1p-like type II pseudouridylate synthase TruA
Length=283
Score = 163 bits (413), Expect = 9e-40, Method: Compositional matrix adjust.
Identities = 100/281 (35%), Positives = 146/281 (51%), Gaps = 55/281 (19%)
Query 1 AQAEDSGIPTVEDTLFRCMERACLIPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVIQGA 60
A +D IPT+E +++ + + LI + +C+ RCGRTD+GVH+AGNY+S+++R
Sbjct 8 AFQKDDKIPTIEGEIYKALLQLRLIKDIDSCDLVRCGRTDRGVHSAGNYISVNLRTRAKN 67
Query 61 AQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFF--GTAGLDIS 118
+ + Y+ +LN +LP DIRV+ +V P F ARFDC +R+YKYFF IS
Sbjct 68 LEPYN-----YMEMLNGVLPWDIRVIGYCEVRPDFSARFDCEYRLYKYFFPLNHERTKIS 122
Query 119 R-----------MRAAAKQFLGMHNFLRFCKVDRK--QERSLWRRIIRFDLELQEDG--F 163
+ M AAKQFLG H++ RFCK+D K + ++ R I FD+ ++ F
Sbjct 123 KFPKIPPEQVPLMNVAAKQFLGTHDYRRFCKMDLKNRKNQTYIRTIYEFDINFVDENRTF 182
Query 164 GVATIVGVSFLWHQVRYMMAALMEVGRGAWPAACISTLLREGLEVEAAVEAAKLDAAEES 223
VATI G +FLWHQVR MM L E+G+ + I+ +L
Sbjct 183 AVATIKGSAFLWHQVRLMMGILFEIGQQKFNPEVITQML--------------------- 221
Query 224 ASMTRAGSTEVEELRLATESCCTALHPAPASNLVLYDCCFE 264
T+VE+ +A S A LVL+DC F+
Sbjct 222 --------TDVEQKNIAELS----FQMATELGLVLWDCVFK 250
> tgo:TGME49_020220 hypothetical protein
Length=370
Score = 152 bits (385), Expect = 2e-36, Method: Compositional matrix adjust.
Identities = 75/136 (55%), Positives = 96/136 (70%), Gaps = 1/136 (0%)
Query 71 YVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGLDISRMRAAAKQFLGM 130
Y +LN +LP DIR++A+ V PTFDARFDC RVYKYFF + GLD+ RM AA++F+G
Sbjct 18 YAAVLNAVLPGDIRIVASAPVSPTFDARFDCTGRVYKYFFHSEGLDLQRMHQAAQRFVGE 77
Query 131 HNFLRFCKVDRKQERSLWRRIIRFDLELQEDGF-GVATIVGVSFLWHQVRYMMAALMEVG 189
HNF FCK+D +Q R+ R + F +E DG VATI G SFLWHQVR+MMAAL++VG
Sbjct 78 HNFRYFCKLDPRQYRNPRRTLTSFAVESAGDGVCAVATIAGRSFLWHQVRFMMAALLKVG 137
Query 190 RGAWPAACISTLLREG 205
RG+ + I LL+ G
Sbjct 138 RGSCDESYIVDLLKRG 153
> mmu:67049 Pus3, 2610020J05Rik, 5730412F04Rik; pseudouridine
synthase 3; K01855 [EC:5.4.99.-]
Length=481
Score = 143 bits (360), Expect = 1e-33, Method: Compositional matrix adjust.
Identities = 85/224 (37%), Positives = 121/224 (54%), Gaps = 25/224 (11%)
Query 3 AEDSGIPTVEDTLFRCMERACLIPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVIQGAAQ 62
++++ T+E+ LF + + L+ +R N+ RCGRTDKGV A G +SLD+R Q
Sbjct 82 SQENTSNTIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRS-QFPTS 140
Query 63 QDSGGCTV------------YVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFF 110
+DS + Y +LNR+LP DIRVLA V P+F ARF CL R Y+YFF
Sbjct 141 RDSEDSNLKHEADDLAKEIRYTHILNRVLPADIRVLAWAPVEPSFSARFSCLERTYRYFF 200
Query 111 GTAGLDISRMRAAAKQFLGMHNFLRFCKVDRKQERSLWRRII---RFDLELQEDG----- 162
A LDI+ M AA++++G H+F CK+D ++R I + L Q G
Sbjct 201 PRADLDIATMNYAAQKYVGTHDFRNLCKMDVANGVINFQRTILCAQVQLVAQSPGEERRQ 260
Query 163 --FGVAT--IVGVSFLWHQVRYMMAALMEVGRGAWPAACISTLL 202
F + ++G +FL+HQVR MMA L +G+G I LL
Sbjct 261 EPFQLCQFEVIGQAFLYHQVRCMMAILFLIGQGMEKPEIIDELL 304
> sce:YFL001W DEG1, HRM3, PUS3; Deg1p (EC:4.2.1.70); K01855 [EC:5.4.99.-]
Length=442
Score = 143 bits (360), Expect = 1e-33, Method: Compositional matrix adjust.
Identities = 84/199 (42%), Positives = 112/199 (56%), Gaps = 8/199 (4%)
Query 1 AQAEDSGIPTVEDTLFRCMERACLIPNR--HACNFSRCGRTDKGVHAAGNYVSLDMRV-I 57
Q E + +PTVE T+ M + L+P+ FSRCGRTDKGV A +SL++R +
Sbjct 110 VQKEYTPLPTVEGTILEAMNKCKLVPSMVLQDYKFSRCGRTDKGVSAMNQVISLEVRSNL 169
Query 58 QGAAQQDSGGCT---VYVPLLNRLLPPDIRVLAA-TQVPPTFDARFDCLFRVYKYFFGTA 113
Q+D + YV +LN+LLP DIR+ A + PP FDARF C+ R YKY F
Sbjct 170 TDEEQRDPTNDSREIPYVHVLNQLLPDDIRISAVCLRPPPNFDARFSCVHRHYKYIFNGK 229
Query 114 GLDISRMRAAAKQFLGMHNFLRFCKVD-RKQERSLWRRIIRFDLELQEDGFGVATIVGVS 172
L+I +M AA F+G +F FCK+D KQ + R II + + F +VG +
Sbjct 230 NLNIEKMSKAASYFVGERDFRNFCKLDGSKQITNFKRTIISSKILPLSETFYCFDLVGSA 289
Query 173 FLWHQVRYMMAALMEVGRG 191
FLWHQVR MMA L VG+
Sbjct 290 FLWHQVRCMMAILFLVGQS 308
> hsa:83480 PUS3, 2610020J05Rik, FLJ23638; pseudouridylate synthase
3; K01855 [EC:5.4.99.-]
Length=481
Score = 142 bits (359), Expect = 1e-33, Method: Compositional matrix adjust.
Identities = 86/217 (39%), Positives = 115/217 (52%), Gaps = 25/217 (11%)
Query 10 TVEDTLFRCMERACLIPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVIQGAAQQDSGGCT 69
T+E+ LF + + L+ +R N+ RCGRTDKGV A G +SLD+R Q +DS
Sbjct 88 TIEEKLFEALTKTRLVESRQTSNYHRCGRTDKGVSAFGQVISLDLRS-QFPRGRDSEDFN 146
Query 70 V------------YVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGLDI 117
V Y +LNR+LPPDIR+LA V P+F ARF CL R Y+YFF A LDI
Sbjct 147 VKEEANAAAEEIRYTHILNRVLPPDIRILAWAPVEPSFSARFSCLERTYRYFFPRADLDI 206
Query 118 SRMRAAAKQFLGMHNFLRFCKVDRKQERSLWRRII---RFDLELQEDGFG---------V 165
M AA++++G H+F CK+D ++R I + L Q G G
Sbjct 207 VTMDYAAQKYVGTHDFRNLCKMDVANGVINFQRTILSAQVQLVGQSPGEGRWQEPFQLCQ 266
Query 166 ATIVGVSFLWHQVRYMMAALMEVGRGAWPAACISTLL 202
+ G +FL+HQVR MMA L +G+G I LL
Sbjct 267 FEVTGQAFLYHQVRCMMAILFLIGQGMEKPEIIDELL 303
> ath:AT1G34150 tRNA pseudouridine synthase family protein
Length=446
Score = 137 bits (346), Expect = 5e-32, Method: Compositional matrix adjust.
Identities = 80/216 (37%), Positives = 113/216 (52%), Gaps = 16/216 (7%)
Query 3 AEDSGIPTVEDTLFRCMERA-CLIPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVIQGAA 61
AE P++E +F+ +ER L+ ++ +SRCGRTDKGV + G ++L +R +
Sbjct 108 AEAQMEPSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTGQVIALFLRSRLKSP 167
Query 62 QQDSGGCTV----------YVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFG 111
DS YV +LNR LP DIRV+ + P F ARF C R YKYFF
Sbjct 168 PGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFHARFSCYAREYKYFFW 227
Query 112 TAGLDISRMRAAAKQFLGMHNFLRFCKVDRKQERSLWRRIIRFDL-----ELQEDGFGVA 166
L++S M A K+F+G H+F FCK+D RR+ F++ + D
Sbjct 228 RQNLNLSAMDFAGKKFIGEHDFRNFCKMDVANVHCYTRRVTFFEVSPCQNSHEGDQLCTF 287
Query 167 TIVGVSFLWHQVRYMMAALMEVGRGAWPAACISTLL 202
T+ G +FLWHQ+R M+A L +G+G I TLL
Sbjct 288 TMRGSAFLWHQIRCMVAVLFMIGQGVESVDVIDTLL 323
> bbo:BBOV_III005490 17.m07491; tRNA pseudouridine synthase A
family protein
Length=248
Score = 134 bits (337), Expect = 5e-31, Method: Compositional matrix adjust.
Identities = 80/213 (37%), Positives = 107/213 (50%), Gaps = 13/213 (6%)
Query 10 TVEDTLFRCMERACLIPNRHACNFSRCGRTDKGVHAAGNYVSL--------DMRVIQGAA 61
TVE LFR + A + + + RCGRTD+ VHA NY S
Sbjct 36 TVEGHLFRALSLANVASDVLNNGYERCGRTDRDVHALHNYASFFPDPCPPNSPCSTVSPD 95
Query 62 QQDSGGC---TVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGLDIS 118
++ G C + +N+ LP IRV VP F +R +C FRVYKYFF +D+S
Sbjct 96 ERGVGPCYRINTFTKAINKHLPRGIRVNWVKHVPDDFSSRRNCTFRVYKYFFQLGSMDLS 155
Query 119 RMRAAAKQFLGMHNFLRFCKVDRKQERSLWRRIIRFDLELQEDGFGVATIVGVSFLWHQV 178
++ A + FLG HNF +FCK D + + I F++E + VATI G +FLWHQV
Sbjct 156 LIQDACRHFLGQHNFNQFCKTDNRNPKDSHGTIYSFEVERYNELLCVATICGRAFLWHQV 215
Query 179 RYMMAALMEVGRGAWPAACISTLLR--EGLEVE 209
R MM L +VG G P + LL EG + E
Sbjct 216 RCMMGVLFKVGEGIIPGLKVKWLLEHPEGAKFE 248
> xla:100101318 pus3; pseudouridylate synthase 3; K01855 [EC:5.4.99.-]
Length=507
Score = 132 bits (333), Expect = 2e-30, Method: Compositional matrix adjust.
Identities = 77/209 (36%), Positives = 108/209 (51%), Gaps = 28/209 (13%)
Query 10 TVEDTLFRCMERACLIPNRHACNFSRCGRTDKGVHAAGNYVSLDMR-VIQGAAQQDSGGC 68
TVE+T+F + + L+ +R N+ RCGRTD+GV A +SLD+R +G +
Sbjct 84 TVEETVFNALLKTRLVESRQTSNYHRCGRTDRGVSALAQVISLDLRCSAEGKPE------ 137
Query 69 TVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGLDISRMRAAAKQFL 128
YV +LNR+LPPDIRVL+ VPP+F ARF C R Y+Y F A LD+ M AA+
Sbjct 138 IRYVHILNRVLPPDIRVLSWAPVPPSFSARFSCGSRTYRYLFPCAQLDVEAMDKAARLLE 197
Query 129 GMHNFLRFCKVDRKQERSLWRRIIRFDLELQEDGFGVA---------------TIVGVSF 173
G H+F CK+D + ++ F + G I G++F
Sbjct 198 GTHDFRNLCKMD------VANGVLNFTRTVLSARVGPVPEIPGDMGPFQLYHLQITGLAF 251
Query 174 LWHQVRYMMAALMEVGRGAWPAACISTLL 202
L+HQVR +M L VG+G I+ LL
Sbjct 252 LYHQVRCIMGVLFLVGQGKEKPEVITELL 280
> cel:E02H1.3 tag-124; Temporarily Assigned Gene name family member
(tag-124); K01718 pseudouridylate synthase [EC:4.2.1.70]
Length=402
Score = 132 bits (331), Expect = 3e-30, Method: Compositional matrix adjust.
Identities = 77/219 (35%), Positives = 114/219 (52%), Gaps = 26/219 (11%)
Query 10 TVEDTLFRCMERACLIPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVI------------ 57
TVE+ + + + + LI + C+FSRCGRTDKGV A ++ +R +
Sbjct 55 TVENHIMQALIKTHLIEDWTKCDFSRCGRTDKGVSAFKQTAAMVVRSLCPGDSGVFWSDS 114
Query 58 ----QGAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTA 113
Q + SG YV +LN +LP IRV A V TF+ARFDC R YKY F A
Sbjct 115 TQEHQKVDYKASGEELPYVKMLNGVLPKTIRVFAWAPVAQTFNARFDCNRRTYKYSFAKA 174
Query 114 GLDISRMRAAAKQFLGMHNFLRFCKVDRKQER---SLWRRIIRFDLEL-----QEDGFGV 165
L++ +MR A+ +G H+F FC++D ++R S R++ +E + D + +
Sbjct 175 DLNLEKMRQGAELLVGEHDFSNFCQIDMNEKRLLQSYVRKVYEVKVEQVSTHPENDMYSM 234
Query 166 A--TIVGVSFLWHQVRYMMAALMEVGRGAWPAACISTLL 202
T+ G FLWH +RY++ L E+GR + IS LL
Sbjct 235 VELTVSGSGFLWHMIRYIVTILQEIGRENEQPSLISQLL 273
> dre:337487 pus3, fj78b12, wu:fj78b12, zgc:56653; pseudouridylate
synthase 3 (EC:5.4.99.-); K01855 [EC:5.4.99.-]
Length=460
Score = 121 bits (303), Expect = 6e-27, Method: Compositional matrix adjust.
Identities = 89/281 (31%), Positives = 121/281 (43%), Gaps = 59/281 (20%)
Query 10 TVEDTLFRCMERACLIPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVIQGA--------A 61
TVE LF + + LI +R + RCGRTDKGV A +S+D+R Q A
Sbjct 83 TVEARLFEALLKTKLIQDRQTSCYHRCGRTDKGVSAFSQVISIDLRSTQYGGGLGVTVPA 142
Query 62 QQDSGGCTV-----YVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGLD 116
D G T YV +LNR+LP DIR+L V F ARFDC R Y+Y+F LD
Sbjct 143 DVDVKGKTSADELPYVKILNRVLPHDIRILQWAPVESGFSARFDCQSRTYRYYFPRGDLD 202
Query 117 ISRMRAAAKQFLGMHNFLRFCKVDRKQERSLWRRII-----------RFDLELQEDGFGV 165
+ M AAK++ G H+F CK+D ++R I + V
Sbjct 203 LELMAEAAKRYEGTHDFRNLCKMDVGNGVLQFQRTILSASVQPAQLPHPSVHNDPHQLFV 262
Query 166 ATIVGVSFLWHQVRYMMAALMEVGRGAWPAACISTLLREGLEVEAAVEAAKLDAAEESAS 225
+ G++ L+HQVR MMA L+ +G+ I LL
Sbjct 263 FQVKGLALLYHQVRCMMALLLLIGQKLEAPEVIDQLL----------------------- 299
Query 226 MTRAGSTEVEELRLATESCCTALHPAPASNLVLYDCCFEGL 266
+VE+ + +P LVLYDC FEG+
Sbjct 300 -------DVEKNPRKPQYSMAVDYP-----LVLYDCHFEGV 328
> pfa:PFI0420c tRNA pseudouridine synthase, putative (EC:4.2.1.70);
K01718 pseudouridylate synthase [EC:4.2.1.70]
Length=312
Score = 96.7 bits (239), Expect = 1e-19, Method: Compositional matrix adjust.
Identities = 63/191 (32%), Positives = 94/191 (49%), Gaps = 22/191 (11%)
Query 10 TVEDTLFRCMERACLIPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVIQGAAQQDSGGCT 69
TVE+ L + + LI N + SR RTDKGV A N ++L + +
Sbjct 30 TVENKLLENLYKLELIDN-YDTPLSRVSRTDKGVSARINGINLRLNIKYEN--------- 79
Query 70 VYVPLL---------NRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGLDISRM 120
VP+ + +I + V TFDAR +C+ R Y YFF +I +M
Sbjct 80 --VPIFEIEKKYVKELKKKLKNIHIHDIKHVSNTFDARLNCIQRTYVYFFINKNYNIEKM 137
Query 121 RAAAKQFLGMHNFLRFCKVDRKQERSLWRRIIRFDLELQEDGFGVATIVGVSFLWHQVRY 180
+ AAK F+G H+F CK K S R +++FD+ + F I G+SFL++Q+R+
Sbjct 138 KKAAKLFIGEHDFSNCCK-KGKNVTSNRRTVLKFDIRNIDKHFYYFKIKGISFLYNQIRH 196
Query 181 MMAALMEVGRG 191
M+A L VG+G
Sbjct 197 MVAILFLVGKG 207
> dre:494060 pus1, wu:fb98h11, zgc:103516; pseudouridine synthase
1 (EC:5.4.99.12); K06173 tRNA pseudouridine synthase A [EC:5.4.99.12]
Length=376
Score = 83.6 bits (205), Expect = 1e-15, Method: Compositional matrix adjust.
Identities = 68/221 (30%), Positives = 96/221 (43%), Gaps = 47/221 (21%)
Query 2 QAEDSGIPTVEDTLFRCMERACLIPNRHA-----CNFSRCGRTDKGVHAAGNYVSLDMRV 56
A +S T+ED L + A IP HA +F R RTDKGV A G VSL + +
Sbjct 59 NARNSQFKTIEDELVTALVGARCIPEGHAEEMKKMSFQRSARTDKGVSAVGRVVSLKVWM 118
Query 57 IQGAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGL- 115
I+ ++ +N LPP IRVL +V F+++ +C R Y Y T
Sbjct 119 IENILEK-----------INAHLPPRIRVLGCKRVTGGFNSKNNCYARTYSYMLPTVAFS 167
Query 116 -------DIS-RMRAAAKQ--------FLGMHNFLRFCKVDRKQERSLWRRI-------- 151
D S R+ + Q + G HNF F ++ S R I
Sbjct 168 PKDYNQEDTSFRLTSDTLQTVNRLFCLYKGTHNFHNFTSQKGHRDPSAMRYITHMSCGEP 227
Query 152 -IRFDLELQEDGFGVATIVGVSFLWHQVRYMMAALMEVGRG 191
IR D+E F V T+ G SF+ HQ+R M+ ++ V +G
Sbjct 228 FIRKDVE-----FAVITVRGQSFMMHQIRKMIGLVIAVVKG 263
> hsa:80324 PUS1, MGC11268, MLASA1; pseudouridylate synthase 1
(EC:5.4.99.12); K06173 tRNA pseudouridine synthase A [EC:5.4.99.12]
Length=399
Score = 79.7 bits (195), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 63/219 (28%), Positives = 93/219 (42%), Gaps = 37/219 (16%)
Query 6 SGIPTVEDTLFRCMERACLIPNRHA-----CNFSRCGRTDKGVHAAGNYVSLDMRVIQGA 60
S T+ED L + R+ IP H +F RC RTDKGV AAG VSL + +I
Sbjct 79 SQFKTIEDDLVSALVRSGCIPENHGEDMRKMSFQRCARTDKGVSAAGQVVSLKVWLIDDI 138
Query 61 AQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGL----- 115
++ +N LP IR+L +V F+++ C R Y Y T
Sbjct 139 LEK-----------INSHLPSHIRILGLKRVTGGFNSKNRCDARTYCYLLPTFAFAHKDR 187
Query 116 ----DISRMRAAAKQ--------FLGMHNFLRFCKVDRKQERSLWRRIIRFDLE--LQED 161
+ R+ A Q + G HNF F Q+ S R I+ E +
Sbjct 188 DVQDETYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPQDPSACRYILEMYCEEPFVRE 247
Query 162 G--FGVATIVGVSFLWHQVRYMMAALMEVGRGAWPAACI 198
G F V + G SF+ HQ+R M+ ++ + +G P + +
Sbjct 248 GLEFAVIRVKGQSFMMHQIRKMVGLVVAIVKGYAPESVL 286
> ath:AT3G06950 tRNA pseudouridine synthase family protein; K06173
tRNA pseudouridine synthase A [EC:5.4.99.12]
Length=323
Score = 75.9 bits (185), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 64/220 (29%), Positives = 93/220 (42%), Gaps = 44/220 (20%)
Query 9 PTVEDTLFRCMERACLIPNRHACNFSRCGRTDKGVHAAG---------NYVSLDMRVIQG 59
PT++ L + + + + R GRTD GVHA G NY SLD
Sbjct 64 PTIQSMLEKALIQITEL-GRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTSLDS----- 117
Query 60 AAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKY----------- 108
+ LN LLP DIRV + P F ARF +VY+Y
Sbjct 118 -----------FHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPF 166
Query 109 -----FFGTAGLDISRMRAAAKQFLGMHNFLRFCKVDRKQ-ERSLWRRIIRFDLELQEDG 162
+ L+ S+MR AA F+G H+F F R+ + I RFD+ +Q
Sbjct 167 QRHWAYHCAYKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDV-IQMGS 225
Query 163 FGVATIVGVSFLWHQVRYMMAALMEVGRGAWPAACISTLL 202
+ G FL+ QVR M+A L+++G+ A + + +L
Sbjct 226 LLQLEVEGSGFLYRQVRNMVALLIQIGKEALDSDIVPMIL 265
> eco:b2318 truA, asuC, ECK2312, hisT, JW2315, leuK; pseudouridylate
synthase I (EC:4.2.1.70); K06173 tRNA pseudouridine synthase
A [EC:5.4.99.12]
Length=270
Score = 71.6 bits (174), Expect = 4e-12, Method: Compositional matrix adjust.
Identities = 56/183 (30%), Positives = 79/183 (43%), Gaps = 26/183 (14%)
Query 36 CGRTDKGVHAAGNYVSLDMRVIQGAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTF 95
GRTD GVH G V + ++ A G +N LP DI V VP F
Sbjct 56 AGRTDAGVHGTGQVVHFETTALRKDAAWTLG--------VNANLPGDIAVRWVKTVPDDF 107
Query 96 DARFDCLFRVYKYFFGT----------------AGLDISRMRAAAKQFLGMHNFLRFCKV 139
ARF R Y+Y LD RM AA+ LG ++F F V
Sbjct 108 HARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGENDFTSFRAV 167
Query 140 DRKQERSLWRRIIRFDLELQEDGFGVATIVGVSFLWHQVRYMMAALMEVGRGAWPAACIS 199
+ Q R+ WR ++ ++ + + V I +F+ H VR ++ +LMEVG P + I+
Sbjct 168 -QCQSRTPWRNVMHINVT-RHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHNQPESWIA 225
Query 200 TLL 202
LL
Sbjct 226 ELL 228
> mmu:56361 Pus1, A730013B20Rik, MPUS1, mPus1p; pseudouridine
synthase 1 (EC:5.4.99.12); K06173 tRNA pseudouridine synthase
A [EC:5.4.99.12]
Length=441
Score = 65.9 bits (159), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 63/233 (27%), Positives = 92/233 (39%), Gaps = 55/233 (23%)
Query 10 TVEDTLFRCMERACLIPNRHACN-----FSRCGRTDK------------------GVHAA 46
T+ED L + +A IP H + F RC RTDK GV AA
Sbjct 107 TIEDDLVSALVQAGCIPENHGTDMRKMSFQRCARTDKPLRTSACFCLYTGFLFHWGVSAA 166
Query 47 GNYVSLDMRVIQGAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVY 106
G VSL + +I + +N LP IR+L +V F+++ C R Y
Sbjct 167 GQVVSLKVWLIDDILDK-----------INSHLPSHIRILGLKRVTGGFNSKNKCDARTY 215
Query 107 KYFFGTAGL-----DIS----RMRAAAKQ--------FLGMHNFLRFCKVDRKQERSLWR 149
Y T D+ R+ A Q + G HNF F +E S R
Sbjct 216 CYMLPTFAFAHKDRDVQDESYRLSAETLQQVNRLLACYKGTHNFHNFTSQKGPREPSARR 275
Query 150 RIIRFDLE--LQEDG--FGVATIVGVSFLWHQVRYMMAALMEVGRGAWPAACI 198
I+ E +G F V + G SF+ HQ+R M+ ++ + +G P + +
Sbjct 276 YILEMYCEEPFVREGLEFAVIKVKGQSFMMHQIRKMVGLVVAIVKGYAPESVL 328
> ath:AT2G30320 tRNA pseudouridine synthase family protein
Length=510
Score = 65.5 bits (158), Expect = 3e-10, Method: Compositional matrix adjust.
Identities = 49/170 (28%), Positives = 77/170 (45%), Gaps = 22/170 (12%)
Query 2 QAEDSGIPTVEDTLFRCMERACLIPNR-----HACNFSRCGRTDKGVHAAGNYVSLDMRV 56
Q +D I T+E L + +A I + H ++R RTDKGVH+ +SL M +
Sbjct 82 QRDDPSIKTIEGELEVAIYKAGGIRDSNYGDLHKIGWARSSRTDKGVHSLATSISLKMEI 141
Query 57 IQGAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYF------- 109
+ A + D G TV +++ LP +IRV + FD R +C R Y Y
Sbjct 142 PETAWKDDPQG-TVLAKCISKHLPENIRVFSVLPSNRRFDPRRECTLRKYSYLLPVDVLG 200
Query 110 ----FGTAGLD--ISRMRAAAKQFLG---MHNFLRFCKVDRKQERSLWRR 150
F + +D I+ K+F G HN+ + + RK E+ + +R
Sbjct 201 IKNSFTSDEIDYHITDFNEILKEFEGEYPFHNYTQRSRYRRKSEQKIKQR 250
> cel:W06H3.2 pus-1; PseudoUridine Synthase family member (pus-1);
K06173 tRNA pseudouridine synthase A [EC:5.4.99.12]
Length=415
Score = 63.5 bits (153), Expect = 1e-09, Method: Compositional matrix adjust.
Identities = 57/214 (26%), Positives = 79/214 (36%), Gaps = 37/214 (17%)
Query 8 IPTVEDTLFRCMERACLIPNRHACN-----FSRCGRTDKGVHAAGNYVSLDMRVIQGAAQ 62
+PT+E L ME A I F R RTD+ V AA + + Q
Sbjct 55 LPTIESKLLEAMETAGWITTAQKEKPFDFFFQRAARTDRAVSAARQMCGMQLPRDDAKYQ 114
Query 63 QDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKY------FFGTAGLD 116
+ G +LN +LP DIRV + F + C R Y Y F L
Sbjct 115 AEGAG------ILNNILPADIRVFGMRRTTNFFHPQKQCDHRTYSYTCPSFVFAKPTELT 168
Query 117 ISRMRAAAKQ----------FLGMHNFLRFCKVDRKQERSLWRRIIRF----------DL 156
S R + + +LG HNF + + S R II F D
Sbjct 169 NSSFRLSPETLAEVNSILSIYLGTHNFFNYTAKRAYDDMSSNRYIISFECKEPFLFRDDF 228
Query 157 ELQEDGFGVATIVGVSFLWHQVRYMMAALMEVGR 190
++ F I G SF+ HQ+R M+ ++ V R
Sbjct 229 RKEDVEFVQIVIKGQSFVLHQIRKMVGMVITVIR 262
> hsa:126789 PUSL1, FLJ90811; pseudouridylate synthase-like 1
Length=303
Score = 60.8 bits (146), Expect = 9e-09, Method: Compositional matrix adjust.
Identities = 66/215 (30%), Positives = 82/215 (38%), Gaps = 56/215 (26%)
Query 31 CNFSRCGRTDKGVHAAGNYVSLDMRVIQGAAQQDSGGCTVYVPLLN-RLLPPDIRVLAAT 89
F+ RTD GVHA N LD +Q + + V LN L P IRVL A
Sbjct 57 VRFTISSRTDAGVHALSNAAHLD---VQRRSGRPPFPPEVLAEALNTHLRHPAIRVLRAF 113
Query 90 QVPPTFDARFDCLFRVYKYFFGTA---------------------GLDISRMRAAAKQFL 128
+VP F AR R Y Y T LD+ M+ AA+ L
Sbjct 114 RVPSDFHARHAATSRTYLYRLATGCHRRDELPVFERNLCWTLPADCLDMVAMQEAAQHLL 173
Query 129 GMHNF----------------LRFCKVDRKQERSLW----RRIIRF-DLELQEDGFGVAT 167
G H+F LR V Q L R +RF +LE +
Sbjct 174 GTHDFSAFQSAGSPVPSPVRTLRRVSVSPGQASPLVTPEESRKLRFWNLEFESQ------ 227
Query 168 IVGVSFLWHQVRYMMAALMEVGRGAWPAACISTLL 202
SFL+ QVR M A L+ VG GA A + T+L
Sbjct 228 ----SFLYRQVRRMTAVLVAVGLGALAPAQVKTIL 258
> sce:YPL212C PUS1; Pus1p (EC:4.2.1.70); K06173 tRNA pseudouridine
synthase A [EC:5.4.99.12]
Length=544
Score = 59.7 bits (143), Expect = 2e-08, Method: Compositional matrix adjust.
Identities = 39/112 (34%), Positives = 50/112 (44%), Gaps = 15/112 (13%)
Query 9 PTVEDTLFRCMERACLIPNRHACN-----FSRCGRTDKGVHAAGNYVSLDMRVIQGAAQQ 63
PT+E LF+ A I ++ + F R RTDKGVHA GN +SL M + +Q
Sbjct 98 PTIESALFKAFVEAGAISKDNSNDLKKNGFMRAARTDKGVHAGGNLISLKMIIEDPDIKQ 157
Query 64 DSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGL 115
+N LP IRV +V FD R C R Y+Y T L
Sbjct 158 K----------INEKLPEGIRVWDIERVNKAFDCRKMCSSRWYEYLLPTYSL 199
Score = 38.1 bits (87), Expect = 0.050, Method: Compositional matrix adjust.
Identities = 36/131 (27%), Positives = 55/131 (41%), Gaps = 26/131 (19%)
Query 91 VPPT---FDARFDCLFRVYKYF---------FGTAGLDISRMRAAAKQFLGMHNFLRFCK 138
VPP FD + +V KY + + +++ RA+ Q+LG HNF F
Sbjct 256 VPPARDEFDINEELYQKVKKYKQLENAHRRRYRISAAKLAKFRASTSQYLGAHNFHNFTL 315
Query 139 VDRKQERSLWRRIIRFDLELQ-EDGFGVA---------TIVGVSFLWHQVRYMMAALMEV 188
+E S IRF +++ D F + I G SF+ HQ+R M++ +
Sbjct 316 GKDFKEPSA----IRFMKDIKVSDPFVIGDAQTEWISIKIHGQSFMLHQIRKMVSMATLI 371
Query 189 GRGAWPAACIS 199
R P IS
Sbjct 372 TRCGCPVERIS 382
> mmu:433813 Pusl1, 2810021I11Rik, AI853843, D18346; pseudouridylate
synthase-like 1 (EC:5.4.99.-)
Length=291
Score = 57.8 bits (138), Expect = 7e-08, Method: Compositional matrix adjust.
Identities = 61/206 (29%), Positives = 77/206 (37%), Gaps = 38/206 (18%)
Query 31 CNFSRCGRTDKGVHAAGNYVSLDMRVIQGAAQQDSGGCTVYVPLLN-RLLPPDIRVLAAT 89
F+ RTD GVHA N LD IQ Q V LN L P IRVL A
Sbjct 57 VRFTISSRTDAGVHALSNAAHLD---IQRRPGQSPFSPEVVAKALNTHLKHPAIRVLKAF 113
Query 90 QVPPTFDARFDCLFRVYKYFFGTA---------------------GLDISRMRAAAKQFL 128
+VP F AR R Y+Y T LD++ M+ AA+ L
Sbjct 114 RVPNDFHARHAATSRTYQYRLATGCSWPNQLPVFEQNVCWALQTEYLDMAAMQEAAQHLL 173
Query 129 GMHNFLRFCKVDRKQERSLWRRIIRFDLE--------LQEDG----FGVATIVGVSFLWH 176
G H+F F ++ R + R + L E F SFL+
Sbjct 174 GTHDFSAFQSAGSPVTNTV-RTLRRVSVSPGPASPFVLPEGSRRLQFWTLEFESQSFLYR 232
Query 177 QVRYMMAALMEVGRGAWPAACISTLL 202
QVR M A L+ VG G + +L
Sbjct 233 QVRRMTAVLVAVGLGILAPTQVKVIL 258
> tpv:TP01_1102 tRNA pseudouridine synthase; K06173 tRNA pseudouridine
synthase A [EC:5.4.99.12]
Length=475
Score = 57.4 bits (137), Expect = 9e-08, Method: Compositional matrix adjust.
Identities = 66/286 (23%), Positives = 99/286 (34%), Gaps = 92/286 (32%)
Query 3 AEDSGIPTVEDTLFRCMERACLIPN-----RHACNFSRCGRTDKGVHAAGNYV----SLD 53
E + TVE TL C+ R I H S+C RTDKGVHAA Y+ ++D
Sbjct 94 GEMDKVKTVEGTLENCLFRIGAINEAFRNMTHKLQMSKCSRTDKGVHAACTYLGGRFNID 153
Query 54 M-----------------------RVIQGAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQ 90
+ ++ Q +V LN +LP DIR +
Sbjct 154 VEDLSDDVVSQAKNHPSESNGELSNNLESTKDQKLSNEDKFVLRLNSVLPEDIRCFKIQR 213
Query 91 VPPTFDARFDCLFRVYKYF----------------------------------------- 109
V FDAR C R Y+Y
Sbjct 214 VTKGFDARTLCSKRKYEYIIPEWILSKKFVVPNEDHENLFRNLELFFEENLKTRDTIKLS 273
Query 110 ------------FGTAGLDISRMRAAAKQFLGMHNFLRFCKVDRKQE-----RSLWRRII 152
+ +D R+ + G H+F F V +K++ R + + I
Sbjct 274 KLNESLYEVKGEYEKTEIDHKRLEEILNFYQGTHSFHNFT-VKQKEDINNSSRFIHKIYI 332
Query 153 RFDLELQEDGFGVATIVGVSFLWHQVRYMMAALMEVGRGAWPAACI 198
+ D +LQE IVG SFL++Q+R M++ ++V P +
Sbjct 333 KSD-KLQELDVVKILIVGQSFLYNQIRKMISLALQVYLKIAPKNSL 377
> bbo:BBOV_IV012080 23.m06054; tRNA pseudouridine synthase (EC:4.2.1.70);
K06173 tRNA pseudouridine synthase A [EC:5.4.99.12]
Length=414
Score = 56.6 bits (135), Expect = 1e-07, Method: Compositional matrix adjust.
Identities = 67/276 (24%), Positives = 98/276 (35%), Gaps = 63/276 (22%)
Query 3 AEDSGIPTVEDTLFRCMERACLIPNRHACNF-----SRCGRTDKGVHAAGNYVSLDMRVI 57
A D GI TVE + + I H N S+ RTDKGVHAA Y+ +
Sbjct 49 AGDDGIDTVEARVINALVTTDAIHGSHKNNLGKLQLSKASRTDKGVHAACTYIGGRFELS 108
Query 58 Q-GAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKY-------- 108
A + V LN +LP DIR +V F AR C R+Y+Y
Sbjct 109 HLSAGEPGISKEQCLVSRLNTVLPDDIRCFQVLRVTRGFCARSMCSKRMYEYVIPLSLLR 168
Query 109 -------------FFGTAGL-------------------------------DISRMRAAA 124
F T+ L D+ +++
Sbjct 169 YRYVCGEDDNKRTFEKTSNLMNLHMNSRQNIHCKRLSSVALHEGTTEERDVDMVLLQSIL 228
Query 125 KQFLGMHNFLRFCKVDRKQERSLWRRIIRFDLELQEDGFGV----ATIVGVSFLWHQVRY 180
+ G H+F F + + +E++ R I +E E G+ I G SFL++Q+R
Sbjct 229 SDYCGSHDFKNFTQRQKVEEKTTQRFIHEIKVEGSE-SIGINAVRVIITGQSFLYNQIRK 287
Query 181 MMAALMEVGRGAWPAACISTLLREGLEVEAAVEAAK 216
M+ V G P I L + V + A+
Sbjct 288 MIGLGYAVYLGVAPRCAIKFALSKWHAVNTPLAPAE 323
> xla:443955 pusl1, MGC80334; pseudouridylate synthase-like 1
(EC:5.4.99.-)
Length=298
Score = 56.2 bits (134), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 57/202 (28%), Positives = 83/202 (41%), Gaps = 35/202 (17%)
Query 33 FSRCGRTDKGVHAAGNYVSLDMRVIQGAAQQDSGGCTVYVPLLNRLLPPD-IRVLAATQV 91
F RTD GVHA N +D+ +G + V +N L P+ IR+L A +V
Sbjct 53 FFISSRTDSGVHAICNLAHVDIERAKGKPPFTE---EILVQAMNHHLQPEPIRLLKAIRV 109
Query 92 PPTFDARFDCLFRVYKY-------------FFGTA------GLDISRMRAAAKQFLGMHN 132
F AR + L R Y Y F G GL+++ + A+K LG HN
Sbjct 110 CDNFHARHNALSRTYVYRVAAGCSRPELPVFEGNLCWAIQDGLNVTAINEASKMLLGTHN 169
Query 133 FLRFCKVDRKQE-RSLWRRIIRFDL-----------ELQEDGFGVATIVGVSFLWHQVRY 180
F F + + +S + + + D+ + + F T SFL+ QVR
Sbjct 170 FSAFRSANSENAFKSPIKTLQQADINPSCGILTHHWQSRNLQFWDFTFRARSFLYKQVRR 229
Query 181 MMAALMEVGRGAWPAACISTLL 202
M AL+ VG+G I L
Sbjct 230 MTGALVAVGQGRLTPQQIKEFL 251
> xla:496371 pus1; pseudouridylate synthase 1; K06173 tRNA pseudouridine
synthase A [EC:5.4.99.12]
Length=167
Score = 51.6 bits (122), Expect = 4e-06, Method: Compositional matrix adjust.
Identities = 31/88 (35%), Positives = 43/88 (48%), Gaps = 16/88 (18%)
Query 6 SGIPTVEDTLFRCMERACLIPNRHA-----CNFSRCGRTDKGVHAAGNYVSLDMRVIQGA 60
S T+ED L + + + IP+ HA +F RC RTDKGV AAG VSL + +I
Sbjct 78 SQFKTIEDELVQALVLSECIPDNHAEDMKKMSFQRCARTDKGVSAAGQVVSLKIWLIDNV 137
Query 61 AQQDSGGCTVYVPLLNRLLPPDIRVLAA 88
++ +N LP IR+L
Sbjct 138 VEK-----------INENLPSHIRILVG 154
> cpv:cgd8_510 Pus1p-like type II pseudousynthas TruA ; K06173
tRNA pseudouridine synthase A [EC:5.4.99.12]
Length=501
Score = 47.4 bits (111), Expect = 8e-05, Method: Compositional matrix adjust.
Identities = 26/96 (27%), Positives = 48/96 (50%), Gaps = 7/96 (7%)
Query 110 FGTAGLDISRMRAAAKQFLGMHNFLRFCKVDRKQERSLWRRIIRFDL------ELQEDGF 163
F + D+ R ++ +++G H+F F ++ + WR I + E++ +
Sbjct 323 FSLSDEDLKRFQSILNEYIGTHSFHNFTSKSSYEDSTSWRHIEEIKVVKVSQNEIKGEIN 382
Query 164 GVATIV-GVSFLWHQVRYMMAALMEVGRGAWPAACI 198
+ I+ G SFL HQ+R M+A +E+ RG P+ I
Sbjct 383 LIKIIIKGQSFLLHQIRKMVALAIEIFRGTAPSNGI 418
> dre:555828 MGC80334 protein-like
Length=290
Score = 47.4 bits (111), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 61/216 (28%), Positives = 80/216 (37%), Gaps = 46/216 (21%)
Query 24 LIPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVIQGAAQQDSGGCTVYVPLLN-RLLPPD 82
L P C S RTD GVHA N +D IQ A + + LN +L
Sbjct 45 LKPVNEVC-VSISSRTDTGVHALCNSAHVD---IQRRADKPPLSEQSLLEALNFQLKEEP 100
Query 83 IRVLAATQVPPTFDARFDCLFRVYKYFFGTA--------------------GLDISRMRA 122
IR+ A +V F AR+ R Y Y L+I MR
Sbjct 101 IRITRAYRVHSDFHARYQAQSRTYVYRLALGCQHHTLMPLTEKDLCWALRDTLNIDAMRE 160
Query 123 AAKQFLGMHNFLRF----------CKV---DRKQ---ERSLWRRIIRFDLELQEDGFGVA 166
A+ LG H+F F C V D Q S +R + D++ E
Sbjct 161 ASALLLGTHDFSTFRALSSDAPFKCPVKTLDLAQLEPGESFSQRHFQRDIQFWE-----L 215
Query 167 TIVGVSFLWHQVRYMMAALMEVGRGAWPAACISTLL 202
T SFL+ QVR M L+ +G+G + I LL
Sbjct 216 TFKSRSFLYRQVRRMTGVLVAIGQGRLSVSKIQDLL 251
> sce:YGL063W PUS2; Mitochondrial tRNA:pseudouridine synthase;
acts at positions 27 and 28, but not at position 72; efficiently
and rapidly targeted to mitochondria, specifically dedicated
to mitochondrial tRNA modification (EC:4.2.1.70); K01718
pseudouridylate synthase [EC:4.2.1.70]
Length=370
Score = 47.0 bits (110), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 33/91 (36%), Positives = 45/91 (49%), Gaps = 13/91 (14%)
Query 20 ERACLIPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVIQGAAQQDSGGCTVYVPLLNRLL 79
E L P +++ F RTDKGVHA N +SL + + ++D+ V LN L
Sbjct 38 EENSLAPKKNS--FMAAARTDKGVHAMLNLLSLKITL-----REDT------VAKLNAAL 84
Query 80 PPDIRVLAATQVPPTFDARFDCLFRVYKYFF 110
PP+IRV V F+AR C R Y+Y
Sbjct 85 PPEIRVWGIQPVNKKFNARSACDSRWYQYLI 115
Score = 37.7 bits (86), Expect = 0.066, Method: Compositional matrix adjust.
Identities = 23/84 (27%), Positives = 36/84 (42%), Gaps = 0/84 (0%)
Query 122 AAAKQFLGMHNFLRFCKVDRKQERSLWRRIIRFDLELQEDGFGVATIVGVSFLWHQVRYM 181
AA ++++G HNF F + S R I + + G+ I G SF+ HQ+R M
Sbjct 205 AAMQEYVGTHNFHNFTTGKLWGDPSAQRHIKKVVVSQASPGWICVRIHGQSFMLHQIRRM 264
Query 182 MAALMEVGRGAWPAACISTLLREG 205
+A + R P + G
Sbjct 265 VALAVLAARCQLPPNIVRNYFNAG 288
> ath:AT1G20370 tRNA pseudouridine synthase family protein; K06173
tRNA pseudouridine synthase A [EC:5.4.99.12]
Length=549
Score = 47.0 bits (110), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 36/118 (30%), Positives = 51/118 (43%), Gaps = 15/118 (12%)
Query 4 EDSGIPTVEDTLFRCMERACLIPNR-----HACNFSRCGRTDKGVHAAGNYVSLDMRVIQ 58
++ G T+E L + A +P+ ++R RTDKGV A G +V+
Sbjct 76 KNPGAKTIEGELEEALFHAGAVPDADRNKPRNYEWARSARTDKGVSAVG-------QVVS 128
Query 59 GAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGLD 116
G D G +V LN LP IRV +V P+F ++ C R Y Y LD
Sbjct 129 GRFYVDPPG---FVERLNSKLPDQIRVFGYKRVAPSFSSKKFCDRRRYVYLIPVFALD 183
Score = 40.8 bits (94), Expect = 0.008, Method: Compositional matrix adjust.
Identities = 21/86 (24%), Positives = 40/86 (46%), Gaps = 4/86 (4%)
Query 127 FLGMHNFLRFCKVDRKQERSLWRRIIRFD----LELQEDGFGVATIVGVSFLWHQVRYMM 182
++G HNF F + + + R I+ F+ + L F +VG SF+ HQ+R M+
Sbjct 328 YVGSHNFHNFTTRTKAADPAANRYILSFNANTVINLDGKDFVKCEVVGQSFMLHQIRKMI 387
Query 183 AALMEVGRGAWPAACISTLLREGLEV 208
+ + R P + I ++ + +
Sbjct 388 GLAVAIMRNYAPESLIEASFKKDVRI 413
> ath:AT1G76120 tRNA pseudouridine synthase family protein; K06173
tRNA pseudouridine synthase A [EC:5.4.99.12]
Length=463
Score = 46.6 bits (109), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 36/118 (30%), Positives = 50/118 (42%), Gaps = 15/118 (12%)
Query 4 EDSGIPTVEDTLFRCMERACLIPNR-----HACNFSRCGRTDKGVHAAGNYVSLDMRVIQ 58
++ G T+E L + A +P +F+R RTDKGV A G +V+
Sbjct 40 KNPGAKTIEGELEEALFHAGAVPESIRGKPKLYDFARSARTDKGVSAVG-------QVVS 92
Query 59 GAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGLD 116
G D G +V LN LP IR+ V P+F ++ C R Y Y LD
Sbjct 93 GRFIVDPLG---FVNRLNSNLPNQIRIFGYKHVTPSFSSKKFCDRRRYVYLLPVFALD 147
Score = 35.8 bits (81), Expect = 0.26, Method: Compositional matrix adjust.
Identities = 21/86 (24%), Positives = 41/86 (47%), Gaps = 4/86 (4%)
Query 127 FLGMHNFLRFCKVDRKQERSLWRRIIRF--DLELQEDG--FGVATIVGVSFLWHQVRYMM 182
++G +NF F + + + R+II F + + DG F ++G SF+ HQ+R MM
Sbjct 275 YVGSYNFHNFTTRTKADDPTANRQIISFTANTVINLDGIDFIKCEVLGKSFMLHQIRKMM 334
Query 183 AALMEVGRGAWPAACISTLLREGLEV 208
+ + R + I + + + +
Sbjct 335 GLAVAIMRNCASESLIQSAFSKDVNI 360
> bbo:BBOV_II003150 18.m06263; RNA pseudouridine synthase A 2
Length=295
Score = 45.4 bits (106), Expect = 4e-04, Method: Compositional matrix adjust.
Identities = 31/77 (40%), Positives = 36/77 (46%), Gaps = 10/77 (12%)
Query 36 CGRTDKGVHA---AGNYVSLDMRVIQGAAQQDSGGCTVYVPLLNRLLPPDIRVLAATQVP 92
RTDKGVHA A Y+S D +G + +NRLLP DIRV A P
Sbjct 160 SSRTDKGVHATETACQYLSFD------KEPPYNGDIDAIMDKVNRLLPDDIRVTAMIHAP 213
Query 93 P-TFDARFDCLFRVYKY 108
F RFD L + Y Y
Sbjct 214 TLDFHVRFDNLGKHYTY 230
> pfa:PF08_0123 pseudouridylate synthase, putative (EC:4.2.1.70);
K06173 tRNA pseudouridine synthase A [EC:5.4.99.12]
Length=556
Score = 42.7 bits (99), Expect = 0.002, Method: Compositional matrix adjust.
Identities = 26/76 (34%), Positives = 40/76 (52%), Gaps = 5/76 (6%)
Query 126 QFLGMHNFLRFCKVDRKQERSLWRRIIRFDL---ELQEDGFGVATIVGVSFLWHQVRYMM 182
++G HNF F K +K +++ +R I FD+ +L + F I+G SFL HQ+R M+
Sbjct 386 NYVGYHNFHCFTK--KKIDQTTYRFIKYFDVSTVKLFDYHFLSVKILGQSFLMHQIRKMI 443
Query 183 AALMEVGRGAWPAACI 198
+E R A I
Sbjct 444 TLAVETYRKATSINSI 459
Score = 42.0 bits (97), Expect = 0.004, Method: Compositional matrix adjust.
Identities = 24/78 (30%), Positives = 39/78 (50%), Gaps = 5/78 (6%)
Query 74 LLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFFGTAGLDISRMRAAAKQ-----FL 128
LLN LP DI+ +V +FDAR C FR Y+Y F L ++ K+ L
Sbjct 222 LLNDHLPCDIKCFKIYKVTKSFDARKFCSFRFYEYLFPVYVLSEVQVNEKYKETFHQAIL 281
Query 129 GMHNFLRFCKVDRKQERS 146
+ +++ CK +++ R+
Sbjct 282 DIDEYVQRCKEEKRTRRN 299
> pfa:PFE0815w tRNA pseudouridine synthase, putative
Length=681
Score = 42.0 bits (97), Expect = 0.004, Method: Compositional matrix adjust.
Identities = 25/82 (30%), Positives = 42/82 (51%), Gaps = 8/82 (9%)
Query 116 DISRMRAAAKQFLGMHNFLRFCKVDRKQERSLWRRIIRF------DLELQEDGFGVATIV 169
DI++++ AK F+G HNF F + E+ R+I F D+ ++ I
Sbjct 552 DINKIKECAKLFIGHHNFECFRGTLKGTEK--LRKINTFCTIHFLDVYELKNNLYQFVIQ 609
Query 170 GVSFLWHQVRYMMAALMEVGRG 191
G FL+H +R ++ L++VG G
Sbjct 610 GDRFLYHMIRIIVGTLVQVGVG 631
Score = 32.7 bits (73), Expect = 2.5, Method: Compositional matrix adjust.
Identities = 28/86 (32%), Positives = 39/86 (45%), Gaps = 6/86 (6%)
Query 25 IPNRHACNFSRCGRTDKGVHAAGNYVSLDMRVIQGAAQQDSGGCTVYVP-LLNRLLPPDI 83
I N F RTDKGVHA Y+ + I + G ++ LN LL DI
Sbjct 213 IINNKPFEFIGVSRTDKGVHAKE-YIC---QYISYEKEPPCDGDMEHIKRSLNSLLNKDI 268
Query 84 RVLAATQVP-PTFDARFDCLFRVYKY 108
++LA + P F+ RF ++Y Y
Sbjct 269 KILAVLKSPHDHFNIRFHNSGKIYTY 294
> tgo:TGME49_024200 hypothetical protein ; K06173 tRNA pseudouridine
synthase A [EC:5.4.99.12]
Length=904
Score = 40.8 bits (94), Expect = 0.007, Method: Compositional matrix adjust.
Identities = 29/95 (30%), Positives = 44/95 (46%), Gaps = 8/95 (8%)
Query 117 ISRMRAAAKQFLGMHNFLRFCKVDRKQERS---LWRRIIRF-----DLELQEDGFGVATI 168
+ ++RA K F G H F F K + ++ S R I R L+ D V +
Sbjct 706 VEKLRAVFKVFEGTHAFQNFTKRGKHKDSSPAAFKRHIHRTAVSQAHLDGVSDDVVVIEL 765
Query 169 VGVSFLWHQVRYMMAALMEVGRGAWPAACISTLLR 203
G SFL++Q+R M+ +EV RG ++ LR
Sbjct 766 EGQSFLFNQIRKMVGIAVEVCRGTATVTSLTDALR 800
Score = 38.1 bits (87), Expect = 0.051, Method: Compositional matrix adjust.
Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 0/41 (0%)
Query 70 VYVPLLNRLLPPDIRVLAATQVPPTFDARFDCLFRVYKYFF 110
++ LN LPPDIR A +V FDAR C R Y +
Sbjct 447 AFLARLNAALPPDIRCFAVRRVTKNFDARVSCSRRRYAFLL 487
Score = 32.3 bits (72), Expect = 3.1, Method: Compositional matrix adjust.
Identities = 14/23 (60%), Positives = 17/23 (73%), Gaps = 0/23 (0%)
Query 32 NFSRCGRTDKGVHAAGNYVSLDM 54
+SR RTD+GVHAA N VSL +
Sbjct 309 QWSRAARTDRGVHAACNVVSLKL 331
> tgo:TGME49_011450 hypothetical protein
Length=1509
Score = 32.3 bits (72), Expect = 3.0, Method: Compositional matrix adjust.
Identities = 21/57 (36%), Positives = 28/57 (49%), Gaps = 23/57 (40%)
Query 38 RTDKGVHAAG---NYVSLDMRVIQGAAQQDSGGCTVYVPL------LNRLLPPDIRV 85
RTD+GVHAAG YVS +++PL +NR LPPD+R+
Sbjct 873 RTDRGVHAAGAICQYVSYYH--------------PLHIPLESFAAGVNRRLPPDVRI 915
Lambda K H
0.323 0.135 0.419
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 16336261752
Database: egene_temp_file_orthology_annotation_similarity_blast_database_866
Posted date: Sep 17, 2011 2:57 PM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40