bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
164,496 sequences; 82,071,388 total letters
Query= Emax_3353_orf1
Length=70
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_118650 transhydrogenase, putative (EC:1.6.1.2); K00... 63.2 2e-10
cpv:cgd8_2330 pyridine nucleotide/ NAD(P) transhydrogenase alp... 49.3 3e-06
pfa:PF14_0508 pyridine nucleotide transhydrogenase, putative (... 44.3 1e-04
tgo:TGME49_101210 NAD(P) transhydrogenase, alpha subunit, puta... 34.7 0.071
dre:100331681 MGC83563 protein-like 33.5 0.15
hsa:23530 NNT, MGC126502, MGC126503; nicotinamide nucleotide t... 32.7 0.25
dre:406619 nnt, wu:fa20d10, wu:fc86a04, zgc:76979; nicotinamid... 32.3 0.34
cel:C15H9.1 nnt-1; Nicotinamide Nucleotide Transhydrogenase fa... 32.3 0.39
tgo:TGME49_003500 alanine dehydrogenase, putative (EC:1.4.1.1) 32.0 0.46
eco:b1603 pntA, ECK1598, JW1595; pyridine nucleotide transhydr... 30.8 1.1
xla:447528 nnt, MGC83563; nicotinamide nucleotide transhydroge... 30.8 1.1
mmu:18115 Nnt, 4930423F13Rik, AI323702, BB168308; nicotinamide... 29.6 2.3
tgo:TGME49_055420 hypothetical protein 28.1 7.2
> tgo:TGME49_118650 transhydrogenase, putative (EC:1.6.1.2); K00322
NAD(P) transhydrogenase [EC:1.6.1.1]
Length=1013
Score = 63.2 bits (152), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 36/69 (52%), Positives = 47/69 (68%), Gaps = 2/69 (2%)
Query 4 KSTEEMERRLNTTPKESSGLGVGVLKDTNG--SVMVPLAPKFVPKLRRMAFRVIVESGAG 61
++ EE R T S VGVLKD+NG + +V LAP+FV +LR+ AFRVIVESGAG
Sbjct 476 RALEEGRRETPPTTWPSPKRTVGVLKDSNGRGTPLVSLAPRFVKRLRKQAFRVIVESGAG 535
Query 62 VDAGFSDEE 70
+A FSD++
Sbjct 536 AEANFSDDD 544
> cpv:cgd8_2330 pyridine nucleotide/ NAD(P) transhydrogenase alpha
plus beta subunits, duplicated gene, possible signal peptide
plus 12 transmembrane regions ; K00322 NAD(P) transhydrogenase
[EC:1.6.1.1]
Length=1143
Score = 49.3 bits (116), Expect = 3e-06, Method: Compositional matrix adjust.
Identities = 24/49 (48%), Positives = 34/49 (69%), Gaps = 1/49 (2%)
Query 23 LGVGVLK-DTNGSVMVPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDEE 70
+ +GVLK D +V +AP FV KLR++ FRV+VESGAG + F D++
Sbjct 586 MTIGVLKEDLPSEKLVAIAPNFVKKLRKLGFRVLVESGAGTKSQFDDQK 634
> pfa:PF14_0508 pyridine nucleotide transhydrogenase, putative
(EC:1.6.1.2); K00322 NAD(P) transhydrogenase [EC:1.6.1.1]
Length=1176
Score = 44.3 bits (103), Expect = 1e-04, Method: Composition-based stats.
Identities = 19/42 (45%), Positives = 29/42 (69%), Gaps = 1/42 (2%)
Query 29 KDTNGSVMVPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDEE 70
+D N S+ VP++PKF+PKLR M FR++VE G + ++E
Sbjct 648 RDVNLSI-VPISPKFIPKLRLMGFRILVERDIGTNILMQNDE 688
> tgo:TGME49_101210 NAD(P) transhydrogenase, alpha subunit, putative
(EC:1.6.1.2)
Length=1165
Score = 34.7 bits (78), Expect = 0.071, Method: Compositional matrix adjust.
Identities = 16/34 (47%), Positives = 20/34 (58%), Gaps = 0/34 (0%)
Query 37 VPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDEE 70
V + P V KLR + I+E AGV AGF DE+
Sbjct 544 VAMTPSVVKKLRELQLGCIIEKQAGVSAGFLDEQ 577
> dre:100331681 MGC83563 protein-like
Length=142
Score = 33.5 bits (75), Expect = 0.15, Method: Compositional matrix adjust.
Identities = 17/34 (50%), Positives = 21/34 (61%), Gaps = 0/34 (0%)
Query 37 VPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDEE 70
V ++P V L + F V VESGAG A FSDE+
Sbjct 56 VSVSPAGVELLVKQGFSVCVESGAGAAAQFSDEQ 89
> hsa:23530 NNT, MGC126502, MGC126503; nicotinamide nucleotide
transhydrogenase (EC:1.6.1.2); K00323 NAD(P) transhydrogenase
[EC:1.6.1.2]
Length=1086
Score = 32.7 bits (73), Expect = 0.25, Method: Composition-based stats.
Identities = 21/48 (43%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Query 23 LGVGVLKDT-NGSVMVPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDE 69
L VGV K+ V L+P V L + F V+VESGAG + FSD+
Sbjct 57 LTVGVPKEIFQNEKRVALSPAGVQNLVKQGFNVVVESGAGEASKFSDD 104
> dre:406619 nnt, wu:fa20d10, wu:fc86a04, zgc:76979; nicotinamide
nucleotide transhydrogenase (EC:1.6.1.2); K00323 NAD(P)
transhydrogenase [EC:1.6.1.2]
Length=1079
Score = 32.3 bits (72), Expect = 0.34, Method: Compositional matrix adjust.
Identities = 16/33 (48%), Positives = 21/33 (63%), Gaps = 0/33 (0%)
Query 37 VPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDE 69
V ++P V L + F V+VESGAG A FSD+
Sbjct 68 VAISPAGVEALIKQGFNVVVESGAGESAKFSDD 100
> cel:C15H9.1 nnt-1; Nicotinamide Nucleotide Transhydrogenase
family member (nnt-1); K00323 NAD(P) transhydrogenase [EC:1.6.1.2]
Length=1041
Score = 32.3 bits (72), Expect = 0.39, Method: Compositional matrix adjust.
Identities = 15/34 (44%), Positives = 22/34 (64%), Gaps = 0/34 (0%)
Query 37 VPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDEE 70
V L+P V L++ V++E AGV AG+S+EE
Sbjct 44 VSLSPNGVALLKKNGISVLIEENAGVLAGYSNEE 77
> tgo:TGME49_003500 alanine dehydrogenase, putative (EC:1.4.1.1)
Length=462
Score = 32.0 bits (71), Expect = 0.46, Method: Composition-based stats.
Identities = 15/33 (45%), Positives = 22/33 (66%), Gaps = 0/33 (0%)
Query 37 VPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDE 69
V L P V +L +V+V++GAGV +GF+DE
Sbjct 43 VALTPATVAELVNNGHKVVVQTGAGVASGFTDE 75
> eco:b1603 pntA, ECK1598, JW1595; pyridine nucleotide transhydrogenase,
alpha subunit (EC:1.6.1.2); K00324 NAD(P) transhydrogenase
subunit alpha [EC:1.6.1.2]
Length=510
Score = 30.8 bits (68), Expect = 1.1, Method: Compositional matrix adjust.
Identities = 16/33 (48%), Positives = 20/33 (60%), Gaps = 0/33 (0%)
Query 37 VPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDE 69
V PK V +L ++ F V VESGAG A F D+
Sbjct 16 VAATPKTVEQLLKLGFTVAVESGAGQLASFDDK 48
> xla:447528 nnt, MGC83563; nicotinamide nucleotide transhydrogenase
(EC:1.6.1.2); K00323 NAD(P) transhydrogenase [EC:1.6.1.2]
Length=1086
Score = 30.8 bits (68), Expect = 1.1, Method: Composition-based stats.
Identities = 15/33 (45%), Positives = 21/33 (63%), Gaps = 0/33 (0%)
Query 37 VPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDE 69
V L+P V L + F V+VE+GAG + FSD+
Sbjct 72 VALSPAGVQALVKQGFNVVVETGAGEASKFSDD 104
> mmu:18115 Nnt, 4930423F13Rik, AI323702, BB168308; nicotinamide
nucleotide transhydrogenase (EC:1.6.1.2); K00323 NAD(P) transhydrogenase
[EC:1.6.1.2]
Length=1086
Score = 29.6 bits (65), Expect = 2.3, Method: Compositional matrix adjust.
Identities = 15/33 (45%), Positives = 20/33 (60%), Gaps = 0/33 (0%)
Query 37 VPLAPKFVPKLRRMAFRVIVESGAGVDAGFSDE 69
V L+P V L + F V+VESGAG + F D+
Sbjct 72 VALSPAGVQALVKQGFNVVVESGAGEASKFPDD 104
> tgo:TGME49_055420 hypothetical protein
Length=278
Score = 28.1 bits (61), Expect = 7.2, Method: Compositional matrix adjust.
Identities = 15/47 (31%), Positives = 24/47 (51%), Gaps = 0/47 (0%)
Query 15 TTPKESSGLGVGVLKDTNGSVMVPLAPKFVPKLRRMAFRVIVESGAG 61
TT S GL +LK+ +VP A +P +M +V ++ G+G
Sbjct 220 TTEMSSYGLLYDLLKNVKAVAVVPSASTLIPIGEQMDSKVFIQRGSG 266
Lambda K H
0.311 0.130 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2024947620
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
Posted date: Sep 17, 2011 11:19 AM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40