bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
164,496 sequences; 82,071,388 total letters
Query= Emax_3113_orf2
Length=157
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_050830 26S proteasome regulatory particle non-ATPas... 77.8 1e-14
pfa:PFC0520w 26S proteasome regulatory subunit S14, putative; ... 54.3 2e-07
ath:AT1G64520 RPN12a; RPN12a (Regulatory Particle non-ATPase 1... 46.2 4e-05
xla:414721 psmd8-a, hip6, hypf, nin1p, psmd8, psmd8a, rpn12; p... 46.2 5e-05
hsa:5714 PSMD8, HIP6, HYPF, MGC1660, Nin1p, Rpn12, S14, p31; p... 44.7 1e-04
mmu:57296 Psmd8, 6720456J22Rik, AA407360, AL033291, AL033322, ... 44.3 2e-04
ath:AT5G42040 RPN12b; RPN12b (Regulatory Particle Non-ATPase 1... 43.5 3e-04
dre:415221 psmd8, fa93g07, fb17c09, fb49a10, wu:fa93g07, wu:fb... 43.5 3e-04
> tgo:TGME49_050830 26S proteasome regulatory particle non-ATPase
subunit, putative ; K03031 26S proteasome regulatory subunit
N12
Length=415
Score = 77.8 bits (190), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 56/142 (39%), Positives = 78/142 (54%), Gaps = 9/142 (6%)
Query 19 DMMTETAAA---FQRHMNMLLPYYCDLAHVLPPSPMQQELQCLQLLHYLAGDCIAELHTA 75
DM+ E A F+RHM MLLPYY D A +LPPS EL CL LL++LA + I E H A
Sbjct 97 DMVDEPLAGEEDFERHMLMLLPYYTDYADLLPPSEHHSELLCLYLLYFLACNRIGEFHMA 156
Query 76 YDRLPEEIQRSPYIVTVMRLEQQLMSGDYEAVMKQQQQQLQQQQQQQQQQQQQQQQVCLP 135
L ++ P + V+ LEQ L+ G Y + LQ+ + + + + LP
Sbjct 157 LQLLAPQVCADPLVAEVLALEQHLIEGSYAFI-----SDLQRSEGRGVSGSKMKIGAALP 211
Query 136 RSAAVFLSQLTKTVQQQIAATL 157
SA VF+ +L KTV+ + AA+L
Sbjct 212 -SAHVFIEKLVKTVRLKAAASL 232
> pfa:PFC0520w 26S proteasome regulatory subunit S14, putative;
K03031 26S proteasome regulatory subunit N12
Length=304
Score = 54.3 bits (129), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 27/87 (31%), Positives = 44/87 (50%), Gaps = 0/87 (0%)
Query 27 AFQRHMNMLLPYYCDLAHVLPPSPMQQELQCLQLLHYLAGDCIAELHTAYDRLPEEIQRS 86
+F ++ L YY D +LP S Q + L LL+ LA + I + H + LP E
Sbjct 109 SFNIYIAQLFIYYFDYKDILPKSKKQNAIVGLYLLYLLASNSIGDFHMTLEILPLEDHND 168
Query 87 PYIVTVMRLEQQLMSGDYEAVMKQQQQ 113
YI V+ +EQ +M G + V+ +++
Sbjct 169 IYIKYVLNVEQNIMDGFFHHVLTKKEN 195
> ath:AT1G64520 RPN12a; RPN12a (Regulatory Particle non-ATPase
12a); peptidase; K03031 26S proteasome regulatory subunit N12
Length=267
Score = 46.2 bits (108), Expect = 4e-05, Method: Compositional matrix adjust.
Identities = 41/137 (29%), Positives = 57/137 (41%), Gaps = 21/137 (15%)
Query 20 MMTETAAAFQRHMNMLLPYYCDLAHVLPPSPMQQELQCLQLLHYLAGDCIAELHTAYDRL 79
+ TE AF+R L PYY D + +P SP + + L LL L + IAE HT + L
Sbjct 73 VKTEDQDAFERDFFQLKPYYVDARNRIPQSPQENLILGLNLLRLLVQNRIAEFHTELELL 132
Query 80 PEEIQRSPYIVTVMRLEQQLMSGDYEAVMKQQQQQLQQQQQQQQQQQQQQQQVCLPRSAA 139
P I + LEQ M G Y V+ +Q P +
Sbjct 133 SSATLEDPCIKHAVELEQSFMEGAYNRVLSARQTA--------------------PDATY 172
Query 140 V-FLSQLTKTVQQQIAA 155
V F+ L KT++ +IA
Sbjct 173 VYFMDLLAKTIRDEIAG 189
> xla:414721 psmd8-a, hip6, hypf, nin1p, psmd8, psmd8a, rpn12;
proteasome (prosome, macropain) 26S subunit, non-ATPase, 8;
K03031 26S proteasome regulatory subunit N12
Length=271
Score = 46.2 bits (108), Expect = 5e-05, Method: Compositional matrix adjust.
Identities = 33/83 (39%), Positives = 47/83 (56%), Gaps = 1/83 (1%)
Query 27 AFQRHMNMLLPYYCDLAHVLPPSPMQQELQCLQLLHYLAGDCIAELHTAYDRLP-EEIQR 85
+F+R+M L YY D LP S + +L L LL L+ + +A+ HT +RLP ++IQ
Sbjct 84 SFERYMAQLKCYYFDYKDELPESAYKHQLLGLNLLFLLSQNRVADFHTELERLPAKDIQT 143
Query 86 SPYIVTVMRLEQQLMSGDYEAVM 108
+ YI + LEQ LM G Y V
Sbjct 144 NVYIKHPVSLEQYLMEGSYNKVF 166
> hsa:5714 PSMD8, HIP6, HYPF, MGC1660, Nin1p, Rpn12, S14, p31;
proteasome (prosome, macropain) 26S subunit, non-ATPase, 8;
K03031 26S proteasome regulatory subunit N12
Length=350
Score = 44.7 bits (104), Expect = 1e-04, Method: Compositional matrix adjust.
Identities = 34/83 (40%), Positives = 46/83 (55%), Gaps = 1/83 (1%)
Query 27 AFQRHMNMLLPYYCDLAHVLPPSPMQQELQCLQLLHYLAGDCIAELHTAYDRLP-EEIQR 85
+F+R+M L YY D LP S +L L LL L+ + +AE HT +RLP ++IQ
Sbjct 163 SFERYMAQLKCYYFDYKEQLPESAYMHQLLGLNLLFLLSQNRVAEFHTELERLPAKDIQT 222
Query 86 SPYIVTVMRLEQQLMSGDYEAVM 108
+ YI + LEQ LM G Y V
Sbjct 223 NVYIKHPVSLEQYLMEGSYNKVF 245
> mmu:57296 Psmd8, 6720456J22Rik, AA407360, AL033291, AL033322,
AL033323, C76433; proteasome (prosome, macropain) 26S subunit,
non-ATPase, 8; K03031 26S proteasome regulatory subunit
N12
Length=353
Score = 44.3 bits (103), Expect = 2e-04, Method: Compositional matrix adjust.
Identities = 34/83 (40%), Positives = 46/83 (55%), Gaps = 1/83 (1%)
Query 27 AFQRHMNMLLPYYCDLAHVLPPSPMQQELQCLQLLHYLAGDCIAELHTAYDRLP-EEIQR 85
+F+R+M L YY D LP S +L L LL L+ + +AE HT +RLP ++IQ
Sbjct 166 SFERYMAQLKCYYFDYKEQLPESAYMHQLLGLNLLFLLSQNRVAEFHTELERLPAKDIQT 225
Query 86 SPYIVTVMRLEQQLMSGDYEAVM 108
+ YI + LEQ LM G Y V
Sbjct 226 NVYIKHPVSLEQYLMEGSYNKVF 248
> ath:AT5G42040 RPN12b; RPN12b (Regulatory Particle Non-ATPase
12b); peptidase; K03031 26S proteasome regulatory subunit N12
Length=233
Score = 43.5 bits (101), Expect = 3e-04, Method: Compositional matrix adjust.
Identities = 30/110 (27%), Positives = 44/110 (40%), Gaps = 19/110 (17%)
Query 46 LPPSPMQQELQCLQLLHYLAGDCIAELHTAYDRLPEEIQRSPYIVTVMRLEQQLMSGDYE 105
+PPSP + + L LL L + IAE HT L +P I + LEQ M G Y
Sbjct 65 IPPSPQENLIMGLNLLRLLVQNRIAEFHTELGLLSSATLENPCIKHAVELEQSFMEGAYN 124
Query 106 AVMKQQQQQLQQQQQQQQQQQQQQQQVCLPRSAAVFLSQLTKTVQQQIAA 155
V+ +Q + + F+ L KT++ +IA
Sbjct 125 RVLSARQTAPDE-------------------TYVYFMDLLAKTIRDEIAG 155
> dre:415221 psmd8, fa93g07, fb17c09, fb49a10, wu:fa93g07, wu:fb17c09,
wu:fb49a10, zgc:86762; proteasome (prosome, macropain)
26S subunit, non-ATPase, 8; K03031 26S proteasome regulatory
subunit N12
Length=267
Score = 43.5 bits (101), Expect = 3e-04, Method: Compositional matrix adjust.
Identities = 33/93 (35%), Positives = 50/93 (53%), Gaps = 1/93 (1%)
Query 17 LEDMMTETAAAFQRHMNMLLPYYCDLAHVLPPSPMQQELQCLQLLHYLAGDCIAELHTAY 76
L ++ + +F+R+M L YY D LP S + +L L LL L+ + ++E HT
Sbjct 70 LWSILKKDIPSFERYMAQLKCYYFDYKDELPESAYRHQLLGLNLLFLLSQNRVSEFHTEL 129
Query 77 DRLP-EEIQRSPYIVTVMRLEQQLMSGDYEAVM 108
+RL ++IQ + YI + LEQ LM G Y V
Sbjct 130 ERLSAKDIQTNVYIRHPVSLEQYLMEGSYNKVF 162
Lambda K H
0.320 0.128 0.355
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 3516928200
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
Posted date: Sep 17, 2011 11:19 AM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40