bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
164,496 sequences; 82,071,388 total letters
Query= Emax_2810_orf1
Length=74
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_113060 eukaryotic translation initiation factor 2B,... 109 2e-24
cpv:cgd5_1600 translation initiation factor if-2B beta; eIF-2 ... 51.2 8e-07
pfa:PFL2430c eukaryotictranslation initiation factor 2b, subun... 50.1 2e-06
ath:AT3G07300 eukaryotic translation initiation factor 2B fami... 48.9 4e-06
mmu:217715 Eif2b2, AA409345, C85417, EIF-2Bbeta, EIF2B; eukary... 43.9 1e-04
xla:379960 eif2b2, MGC53907; eukaryotic translation initiation... 43.9 1e-04
hsa:8892 EIF2B2, EIF-2Bbeta, EIF2B; eukaryotic translation ini... 43.1 2e-04
sce:YLR291C GCD7; Beta subunit of the translation initiation f... 42.7 3e-04
dre:405839 eif2b2, MGC77512, zgc:77512; eukaryotic translation... 41.2 9e-04
mmu:22390 Wee1; WEE 1 homolog 1 (S. pombe) (EC:2.7.10.2); K066... 29.3 3.2
> tgo:TGME49_113060 eukaryotic translation initiation factor 2B,
putative ; K03754 translation initiation factor eIF-2B subunit
beta
Length=693
Score = 109 bits (273), Expect = 2e-24, Method: Composition-based stats.
Identities = 47/72 (65%), Positives = 63/72 (87%), Gaps = 0/72 (0%)
Query 2 WTAPFVEAFLQQLRRREIRGSRMAAKRTAETLKRVVDAYPWKTTQELVDVVRYIGRRLIC 61
WTAPF+EA + QLRRRE+RGS AA+RTAE L+R+VD YPW+TT EL+DVV+++GRRLI
Sbjct 249 WTAPFIEALILQLRRRELRGSHQAARRTAEVLRRLVDVYPWQTTHELIDVVKHVGRRLIR 308
Query 62 AAPLDLLVANIV 73
A+P++ LVAN++
Sbjct 309 ASPMEFLVANVL 320
> cpv:cgd5_1600 translation initiation factor if-2B beta; eIF-2
; K03754 translation initiation factor eIF-2B subunit beta
Length=362
Score = 51.2 bits (121), Expect = 8e-07, Method: Composition-based stats.
Identities = 21/56 (37%), Positives = 34/56 (60%), Gaps = 0/56 (0%)
Query 10 FLQQLRRREIRGSRMAAKRTAETLKRVVDAYPWKTTQELVDVVRYIGRRLICAAPL 65
++ LRRR++ GS+ K+T E L+++VD Y WK EL+ ++ IG + PL
Sbjct 38 LIELLRRRQLIGSQAQGKKTLEVLRQIVDKYSWKNVNELISNIKEIGSEVASIDPL 93
> pfa:PFL2430c eukaryotictranslation initiation factor 2b, subunit
2, putative; K03754 translation initiation factor eIF-2B
subunit beta
Length=679
Score = 50.1 bits (118), Expect = 2e-06, Method: Composition-based stats.
Identities = 20/72 (27%), Positives = 41/72 (56%), Gaps = 0/72 (0%)
Query 2 WTAPFVEAFLQQLRRREIRGSRMAAKRTAETLKRVVDAYPWKTTQELVDVVRYIGRRLIC 61
W V+ + + I GS K+ AE LK++V+ Y WK +L+++++Y+G+++I
Sbjct 257 WLNGIVDLLKRGFKNGNICGSNSVGKKIAEVLKKIVEIYQWKNVFDLIEIIKYLGKQIIK 316
Query 62 AAPLDLLVANIV 73
+ ++ NI+
Sbjct 317 NNKMFFVIPNII 328
> ath:AT3G07300 eukaryotic translation initiation factor 2B family
protein / eIF-2B family protein; K03754 translation initiation
factor eIF-2B subunit beta
Length=407
Score = 48.9 bits (115), Expect = 4e-06, Method: Composition-based stats.
Identities = 26/70 (37%), Positives = 41/70 (58%), Gaps = 3/70 (4%)
Query 7 VEAFLQQLRRREIRGSRMAAKRTAETLKRVVDAYPWKTTQE---LVDVVRYIGRRLICAA 63
V F+ +LR+R+I GS+ AK T E L+ V+ + L+D V+ +G +LI A
Sbjct 8 VVEFVNKLRKRKIEGSQATAKYTVELLRSVISHQRVPHANQAAALIDAVKAVGEQLIAAN 67
Query 64 PLDLLVANIV 73
P++L V N+V
Sbjct 68 PVELAVGNVV 77
> mmu:217715 Eif2b2, AA409345, C85417, EIF-2Bbeta, EIF2B; eukaryotic
translation initiation factor 2B, subunit 2 beta; K03754
translation initiation factor eIF-2B subunit beta
Length=351
Score = 43.9 bits (102), Expect = 1e-04, Method: Composition-based stats.
Identities = 22/68 (32%), Positives = 38/68 (55%), Gaps = 1/68 (1%)
Query 7 VEAFLQQLRRRE-IRGSRMAAKRTAETLKRVVDAYPWKTTQELVDVVRYIGRRLICAAPL 65
+E F++ L+R R S A+ T L+R++ + W +L+D++R GRR+ A P
Sbjct 15 IEGFVETLKRGGGQRSSEDMARETLGLLRRLITDHHWNNAGDLMDLIRREGRRMTAAQPS 74
Query 66 DLLVANIV 73
+ V N+V
Sbjct 75 ETTVGNMV 82
> xla:379960 eif2b2, MGC53907; eukaryotic translation initiation
factor 2B, subunit 2 beta, 39kDa; K03754 translation initiation
factor eIF-2B subunit beta
Length=305
Score = 43.9 bits (102), Expect = 1e-04, Method: Composition-based stats.
Identities = 23/68 (33%), Positives = 38/68 (55%), Gaps = 1/68 (1%)
Query 7 VEAFLQQLRRREIR-GSRMAAKRTAETLKRVVDAYPWKTTQELVDVVRYIGRRLICAAPL 65
+EAF+ +L+R R S A+ T L+R++ W EL++++R GRR+ A P
Sbjct 15 IEAFVAELKRGGGRPSSDETARETVGLLRRIIAQARWGNAGELMEIIRREGRRMTAAQPS 74
Query 66 DLLVANIV 73
+ V N+V
Sbjct 75 ETTVGNMV 82
> hsa:8892 EIF2B2, EIF-2Bbeta, EIF2B; eukaryotic translation initiation
factor 2B, subunit 2 beta, 39kDa; K03754 translation
initiation factor eIF-2B subunit beta
Length=351
Score = 43.1 bits (100), Expect = 2e-04, Method: Composition-based stats.
Identities = 21/68 (30%), Positives = 39/68 (57%), Gaps = 1/68 (1%)
Query 7 VEAFLQQLRRRE-IRGSRMAAKRTAETLKRVVDAYPWKTTQELVDVVRYIGRRLICAAPL 65
+E+F++ L+R R S A+ T L++++ + W EL++++R GRR+ A P
Sbjct 15 IESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPS 74
Query 66 DLLVANIV 73
+ V N+V
Sbjct 75 ETTVGNMV 82
> sce:YLR291C GCD7; Beta subunit of the translation initiation
factor eIF2B, the guanine-nucleotide exchange factor for eIF2;
activity subsequently regulated by phosphorylated eIF2;
first identified as a negative regulator of GCN4 expression;
K03754 translation initiation factor eIF-2B subunit beta
Length=381
Score = 42.7 bits (99), Expect = 3e-04, Method: Composition-based stats.
Identities = 19/67 (28%), Positives = 36/67 (53%), Gaps = 0/67 (0%)
Query 7 VEAFLQQLRRREIRGSRMAAKRTAETLKRVVDAYPWKTTQELVDVVRYIGRRLICAAPLD 66
++ F+ +L+RR+++GS A T + L R + A W +L++ +R +G L A P
Sbjct 22 IDTFVAKLKRRQVQGSYAIALETLQLLMRFISAARWNHVNDLIEQIRDLGNSLEKAHPTA 81
Query 67 LLVANIV 73
N++
Sbjct 82 FSCGNVI 88
> dre:405839 eif2b2, MGC77512, zgc:77512; eukaryotic translation
initiation factor 2B, subunit 2 beta; K03754 translation
initiation factor eIF-2B subunit beta
Length=355
Score = 41.2 bits (95), Expect = 9e-04, Method: Composition-based stats.
Identities = 20/72 (27%), Positives = 38/72 (52%), Gaps = 5/72 (6%)
Query 7 VEAFLQQLRRREIRGSRM-----AAKRTAETLKRVVDAYPWKTTQELVDVVRYIGRRLIC 61
+E+FL +L+R + A+ T+ L+R+ W + EL++++R GRR+
Sbjct 14 IESFLSELKRCGSGSGSLRGSVETARETSSLLRRITAQARWSSAGELMEIIRKEGRRMTA 73
Query 62 AAPLDLLVANIV 73
A P + V N++
Sbjct 74 AQPSETTVGNMI 85
> mmu:22390 Wee1; WEE 1 homolog 1 (S. pombe) (EC:2.7.10.2); K06632
wee1-like protein kinase [EC:2.7.11.1]
Length=646
Score = 29.3 bits (64), Expect = 3.2, Method: Composition-based stats.
Identities = 15/52 (28%), Positives = 27/52 (51%), Gaps = 0/52 (0%)
Query 8 EAFLQQLRRREIRGSRMAAKRTAETLKRVVDAYPWKTTQELVDVVRYIGRRL 59
E F L ++E++ ++MAAK AE D ++T + R IG+++
Sbjct 586 EKFKNSLLQKELKKAQMAAKVAAEERALFTDRMATRSTTQSNRTSRLIGKKM 637
Lambda K H
0.327 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2007182052
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
Posted date: Sep 17, 2011 11:19 AM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40