bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
164,496 sequences; 82,071,388 total letters
Query= Emax_2173_orf1
Length=86
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_063730 glycerol-3-phosphate dehydrogenase, putative... 79.0 3e-15
pfa:PFC0275w FAD-dependent glycerol-3-phosphate dehydrogenase,... 66.2 2e-11
bbo:BBOV_III000930 17.m07109; FAD-dependent glycerol-3-phospha... 62.8 3e-10
tpv:TP03_0730 glycerol-3-phosphate dehydrogenase (EC:1.1.3.21)... 56.2 2e-08
ath:AT3G10370 SDP6; SDP6 (SUGAR-DEPENDENT 6); glycerol-3-phosp... 55.5 4e-08
cel:T25G3.4 hypothetical protein; K00111 glycerol-3-phosphate ... 54.3 9e-08
xla:444438 gpd2, MGC83596; glycerol-3-phosphate dehydrogenase ... 51.6 6e-07
cel:Y50E8A.6 hypothetical protein 48.9 4e-06
hsa:2820 GPD2, GDH2, GPDM, mGPDH; glycerol-3-phosphate dehydro... 46.2 3e-05
mmu:14571 Gpd2, AA408484, AI448216, AU021455, AW494132, GPDH, ... 45.8 3e-05
sce:YIL155C GUT2; Gut2p (EC:1.1.5.3); K00111 glycerol-3-phosph... 41.6 6e-04
> tgo:TGME49_063730 glycerol-3-phosphate dehydrogenase, putative
(EC:1.1.3.21); K00111 glycerol-3-phosphate dehydrogenase
[EC:1.1.5.3]
Length=653
Score = 79.0 bits (193), Expect = 3e-15, Method: Compositional matrix adjust.
Identities = 40/80 (50%), Positives = 55/80 (68%), Gaps = 0/80 (0%)
Query 7 EELPYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKELGWSAS 66
E P+LKAEV++ RYE ARSVSDV+ARR+ + F+D A ID ++ +M +EL WS++
Sbjct 568 EAFPFLKAEVLYGVRYEMARSVSDVLARRMRLAFVDVAKAEACIDEVARIMGEELKWSSA 627
Query 67 VAAKKKEEALAYAETFTVSP 86
KKKEEA A+ +TF P
Sbjct 628 KLEKKKEEAAAFLKTFRCLP 647
> pfa:PFC0275w FAD-dependent glycerol-3-phosphate dehydrogenase,
putative; K00111 glycerol-3-phosphate dehydrogenase [EC:1.1.5.3]
Length=653
Score = 66.2 bits (160), Expect = 2e-11, Method: Composition-based stats.
Identities = 27/75 (36%), Positives = 49/75 (65%), Gaps = 0/75 (0%)
Query 10 PYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKELGWSASVAA 69
PY++AE+++A RYE A ++SD+I RR + F+D +++ +VID ++ L+ EL W+
Sbjct 575 PYIEAEIIYATRYEFANTISDIIGRRFRLGFIDSQVSNKVIDKIAQLLKNELTWNTEQVN 634
Query 70 KKKEEALAYAETFTV 84
K +EA Y + ++
Sbjct 635 KNVKEAKDYINSLSL 649
> bbo:BBOV_III000930 17.m07109; FAD-dependent glycerol-3-phosphate
dehydrogenase (EC:1.1.99.5); K00111 glycerol-3-phosphate
dehydrogenase [EC:1.1.5.3]
Length=619
Score = 62.8 bits (151), Expect = 3e-10, Method: Compositional matrix adjust.
Identities = 29/77 (37%), Positives = 48/77 (62%), Gaps = 0/77 (0%)
Query 8 ELPYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKELGWSASV 67
+ PYL+ EV++ R E A + D++ARR + FLD K A +DT+ ++M +EL WS+S
Sbjct 540 DFPYLQGEVLYGVRREFACTPLDILARRTRIAFLDNKAAAASLDTVCDIMGRELSWSSSR 599
Query 68 AAKKKEEALAYAETFTV 84
A+ + +A+ + T V
Sbjct 600 RAELRSQAVDFFNTMNV 616
> tpv:TP03_0730 glycerol-3-phosphate dehydrogenase (EC:1.1.3.21);
K00111 glycerol-3-phosphate dehydrogenase [EC:1.1.5.3]
Length=615
Score = 56.2 bits (134), Expect = 2e-08, Method: Composition-based stats.
Identities = 30/86 (34%), Positives = 47/86 (54%), Gaps = 0/86 (0%)
Query 1 LMDRLVEELPYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKE 60
L+ ++ E P+L EVV+ R E A + DV+ARR + FLD K A++ +D + ++M KE
Sbjct 530 LLTKIHENYPFLLGEVVYGIRNELACTPVDVLARRTRLAFLDAKAALQSLDKVVSVMEKE 589
Query 61 LGWSASVAAKKKEEALAYAETFTVSP 86
L W+ EA Y + + P
Sbjct 590 LNWNPKTKKTLYLEAQDYFQQMSHVP 615
> ath:AT3G10370 SDP6; SDP6 (SUGAR-DEPENDENT 6); glycerol-3-phosphate
dehydrogenase (EC:1.1.5.3); K00111 glycerol-3-phosphate
dehydrogenase [EC:1.1.5.3]
Length=629
Score = 55.5 bits (132), Expect = 4e-08, Method: Compositional matrix adjust.
Identities = 30/85 (35%), Positives = 44/85 (51%), Gaps = 0/85 (0%)
Query 1 LMDRLVEELPYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKE 60
L RL P+L+AEV + R+E S D IARR + FLD A R + + ++A E
Sbjct 534 LGKRLAHGHPFLEAEVAYCARHEYCESAVDFIARRCRIAFLDTDAAARALQRVVEILASE 593
Query 61 LGWSASVAAKKKEEALAYAETFTVS 85
W S ++ ++A + ETF S
Sbjct 594 HKWDKSRQKQELQKAKEFLETFKSS 618
> cel:T25G3.4 hypothetical protein; K00111 glycerol-3-phosphate
dehydrogenase [EC:1.1.5.3]
Length=722
Score = 54.3 bits (129), Expect = 9e-08, Method: Composition-based stats.
Identities = 31/77 (40%), Positives = 46/77 (59%), Gaps = 1/77 (1%)
Query 4 RLVEELPYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKELGW 63
RL E PYL AEV +A R E A + DVIARR+ + FL+ A V+ + +M +ELGW
Sbjct 527 RLHPEFPYLDAEVRYAVR-EYACTAIDVIARRMRLAFLNTYAAHEVLPDVVRVMGQELGW 585
Query 64 SASVAAKKKEEALAYAE 80
S++ + E+A + +
Sbjct 586 SSAEQRAQLEKARTFID 602
> xla:444438 gpd2, MGC83596; glycerol-3-phosphate dehydrogenase
2 (mitochondrial) (EC:1.1.5.3); K00111 glycerol-3-phosphate
dehydrogenase [EC:1.1.5.3]
Length=725
Score = 51.6 bits (122), Expect = 6e-07, Method: Compositional matrix adjust.
Identities = 32/79 (40%), Positives = 48/79 (60%), Gaps = 5/79 (6%)
Query 4 RLVEELPYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKELGW 63
RLV E PY++AEV +A R E A + DVI+RR + FL+ + A + + ++MAKEL W
Sbjct 523 RLVSEFPYIEAEVKYALR-EYACTAVDVISRRTRLAFLNVQAAEEALPRIVDIMAKELNW 581
Query 64 SASVAAKKKEEALAYAETF 82
++K+E L A+ F
Sbjct 582 K----EQRKKEELETAKKF 596
> cel:Y50E8A.6 hypothetical protein
Length=609
Score = 48.9 bits (115), Expect = 4e-06, Method: Composition-based stats.
Identities = 26/63 (41%), Positives = 41/63 (65%), Gaps = 1/63 (1%)
Query 4 RLVEELPYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKELGW 63
RL + P+L+AEV +A + E AR +D++ARR + LD + A +V+ + +MA+EL W
Sbjct 515 RLHPDFPFLEAEVRYAVK-EYARIPADILARRTRLSLLDARAARQVLPRIVAIMAEELKW 573
Query 64 SAS 66
S S
Sbjct 574 SPS 576
> hsa:2820 GPD2, GDH2, GPDM, mGPDH; glycerol-3-phosphate dehydrogenase
2 (mitochondrial) (EC:1.1.5.3); K00111 glycerol-3-phosphate
dehydrogenase [EC:1.1.5.3]
Length=727
Score = 46.2 bits (108), Expect = 3e-05, Method: Compositional matrix adjust.
Identities = 29/79 (36%), Positives = 43/79 (54%), Gaps = 5/79 (6%)
Query 4 RLVEELPYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKELGW 63
RLV E PY++AEV + + E A + D+I+RR + FL+ + A + + LM +EL W
Sbjct 525 RLVSEFPYIEAEVKYGIK-EYACTAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELNW 583
Query 64 SASVAAKKKEEALAYAETF 82
KK+E L A F
Sbjct 584 DDY----KKQEQLETARKF 598
> mmu:14571 Gpd2, AA408484, AI448216, AU021455, AW494132, GPDH,
Gdm1, Gpd-m, Gpdh-m, TISP38; glycerol phosphate dehydrogenase
2, mitochondrial (EC:1.1.5.3); K00111 glycerol-3-phosphate
dehydrogenase [EC:1.1.5.3]
Length=727
Score = 45.8 bits (107), Expect = 3e-05, Method: Compositional matrix adjust.
Identities = 29/79 (36%), Positives = 44/79 (55%), Gaps = 5/79 (6%)
Query 4 RLVEELPYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKELGW 63
RLV E PY++AEV + + E A + D+I+RR + FL+ + A + + LM +EL W
Sbjct 525 RLVSEFPYIEAEVKYGIK-EYACTAVDMISRRTRLAFLNVQAAEEALPRIVELMGRELNW 583
Query 64 SASVAAKKKEEALAYAETF 82
S +K+E L A F
Sbjct 584 S----ELRKQEELETATRF 598
> sce:YIL155C GUT2; Gut2p (EC:1.1.5.3); K00111 glycerol-3-phosphate
dehydrogenase [EC:1.1.5.3]
Length=649
Score = 41.6 bits (96), Expect = 6e-04, Method: Compositional matrix adjust.
Identities = 21/75 (28%), Positives = 36/75 (48%), Gaps = 0/75 (0%)
Query 10 PYLKAEVVFACRYEQARSVSDVIARRLPVLFLDQKLAVRVIDTMSNLMAKELGWSASVAA 69
P+ E+ ++ +YE R+ D + RR FLD K A+ + +M E WS
Sbjct 575 PFTIGELKYSMQYEYCRTPLDFLLRRTRFAFLDAKEALNAVHATVKVMGDEFNWSEKKRQ 634
Query 70 KKKEEALAYAETFTV 84
+ E+ + + +TF V
Sbjct 635 WELEKTVNFIKTFGV 649
Lambda K H
0.320 0.131 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2030857360
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
Posted date: Sep 17, 2011 11:19 AM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40