bitscore colors: <40, 40-50 , 50-80, 80-200, >200




           BLASTP 2.2.24+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.



Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
           164,496 sequences; 82,071,388 total letters



Query=  Emax_1662_orf1
Length=56
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  tgo:TGME49_118650  transhydrogenase, putative (EC:1.6.1.2); K00...  68.2    6e-12
  cpv:cgd8_2330  pyridine nucleotide/ NAD(P) transhydrogenase alp...  59.3    3e-09
  pfa:PF14_0508  pyridine nucleotide transhydrogenase, putative (...  56.2    2e-08
  eco:b1603  pntA, ECK1598, JW1595; pyridine nucleotide transhydr...  51.6    7e-07
  cel:C15H9.1  nnt-1; Nicotinamide Nucleotide Transhydrogenase fa...  47.4    1e-05
  tgo:TGME49_101210  NAD(P) transhydrogenase, alpha subunit, puta...  42.7    2e-04
  dre:406619  nnt, wu:fa20d10, wu:fc86a04, zgc:76979; nicotinamid...  40.4    0.001
  xla:447528  nnt, MGC83563; nicotinamide nucleotide transhydroge...  39.3    0.003
  cpv:cgd1_990  pyridine nucleotide/ NAD(P) transhydrogenase alph...  35.0    0.058
  dre:100331743  Nicotinamide Nucleotide Transhydrogenase family ...  34.7    0.077
  tgo:TGME49_115260  alanine dehydrogenase, putative (EC:1.4.1.1)     34.3    0.095
  mmu:71664  Mettl7b, 0610006F02Rik, AI266817; methyltransferase ...  28.1    6.3
  mmu:70152  Mettl7a1, 2210414H16Rik, 3300001H21Rik, Aam-B, Mettl...  28.1    6.4
  mmu:393082  Mettl7a2, Gm921, UbiE2, Ubie; methyltransferase lik...  28.1    6.4
  hsa:196410  METTL7B, MGC17301; methyltransferase like 7B            28.1    7.9


> tgo:TGME49_118650  transhydrogenase, putative (EC:1.6.1.2); K00322 
NAD(P) transhydrogenase [EC:1.6.1.1]
Length=1013

 Score = 68.2 bits (165),  Expect = 6e-12, Method: Composition-based stats.
 Identities = 28/43 (65%), Positives = 37/43 (86%), Gaps = 0/43 (0%)

Query  14   AQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            AQ+++++  + + DV+ICTAAIHGKPSPKLIS DML TM+PGS
Sbjct  734  AQKQMLSRVVPNVDVIICTAAIHGKPSPKLISNDMLATMRPGS  776


> cpv:cgd8_2330  pyridine nucleotide/ NAD(P) transhydrogenase alpha 
plus beta subunits, duplicated gene, possible signal peptide 
plus 12 transmembrane regions ; K00322 NAD(P) transhydrogenase 
[EC:1.6.1.1]
Length=1143

 Score = 59.3 bits (142),  Expect = 3e-09, Method: Composition-based stats.
 Identities = 26/45 (57%), Positives = 35/45 (77%), Gaps = 0/45 (0%)

Query  12   IAAQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            + AQ +L +  I+ CDVVI TA I GKPSPK+I+R+M+ +MKPGS
Sbjct  822  LKAQSKLYSKMIRSCDVVITTALIPGKPSPKIITREMVNSMKPGS  866


> pfa:PF14_0508  pyridine nucleotide transhydrogenase, putative 
(EC:1.6.1.2); K00322 NAD(P) transhydrogenase [EC:1.6.1.1]
Length=1176

 Score = 56.2 bits (134),  Expect = 2e-08, Method: Composition-based stats.
 Identities = 23/45 (51%), Positives = 32/45 (71%), Gaps = 0/45 (0%)

Query  12   IAAQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            I  Q  L    IK CD++IC+A+I GK SPKL++ +M++ MKPGS
Sbjct  879  IKVQSNLFKKIIKKCDILICSASIPGKTSPKLVTTEMIKLMKPGS  923


> eco:b1603  pntA, ECK1598, JW1595; pyridine nucleotide transhydrogenase, 
alpha subunit (EC:1.6.1.2); K00324 NAD(P) transhydrogenase 
subunit alpha [EC:1.6.1.2]
Length=510

 Score = 51.6 bits (122),  Expect = 7e-07, Method: Composition-based stats.
 Identities = 23/45 (51%), Positives = 32/45 (71%), Gaps = 0/45 (0%)

Query  12   IAAQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            I A+ EL A   K  D+++ TA I GKP+PKLI+R+M+ +MK GS
Sbjct  235  IKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMKAGS  279


> cel:C15H9.1  nnt-1; Nicotinamide Nucleotide Transhydrogenase 
family member (nnt-1); K00323 NAD(P) transhydrogenase [EC:1.6.1.2]
Length=1041

 Score = 47.4 bits (111),  Expect = 1e-05, Method: Composition-based stats.
 Identities = 21/45 (46%), Positives = 32/45 (71%), Gaps = 0/45 (0%)

Query  12   IAAQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            I A+ +L A+  K  D++I TA I GK +P LI+ +M+++MKPGS
Sbjct  263  IDAEMKLFADQCKDVDIIITTALIPGKKAPILITEEMIKSMKPGS  307


> tgo:TGME49_101210  NAD(P) transhydrogenase, alpha subunit, putative 
(EC:1.6.1.2)
Length=1165

 Score = 42.7 bits (99),  Expect = 2e-04, Method: Composition-based stats.
 Identities = 18/42 (42%), Positives = 29/42 (69%), Gaps = 0/42 (0%)

Query  15   QRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            + EL A   +  D++I TA++ G+P+PKLI ++ + TMK GS
Sbjct  839  EMELFAEQCREIDILITTASLPGRPAPKLIKKEHVDTMKAGS  880


> dre:406619  nnt, wu:fa20d10, wu:fc86a04, zgc:76979; nicotinamide 
nucleotide transhydrogenase (EC:1.6.1.2); K00323 NAD(P) 
transhydrogenase [EC:1.6.1.2]
Length=1079

 Score = 40.4 bits (93),  Expect = 0.001, Method: Composition-based stats.
 Identities = 19/45 (42%), Positives = 29/45 (64%), Gaps = 0/45 (0%)

Query  12   IAAQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            I A+ +L A      D++I TA I G+ +P LI+++M+ TMK GS
Sbjct  293  IEAEMKLFAKQCLDVDIIITTALIPGRKAPVLITKEMVETMKDGS  337


> xla:447528  nnt, MGC83563; nicotinamide nucleotide transhydrogenase 
(EC:1.6.1.2); K00323 NAD(P) transhydrogenase [EC:1.6.1.2]
Length=1086

 Score = 39.3 bits (90),  Expect = 0.003, Method: Composition-based stats.
 Identities = 18/45 (40%), Positives = 27/45 (60%), Gaps = 0/45 (0%)

Query  12   IAAQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            I A+ +L A   +  D+++ TA I GK +P L  +DM+  MK GS
Sbjct  297  IEAEMKLFAKQCQDVDIIVTTALIPGKTAPILFRKDMIELMKEGS  341


> cpv:cgd1_990  pyridine nucleotide/ NAD(P) transhydrogenase alpha 
plus beta subunits, duplicated gene, 12 transmembrane domain 
(EC:1.6.1.2)
Length=1147

 Score = 35.0 bits (79),  Expect = 0.058, Method: Composition-based stats.
 Identities = 17/45 (37%), Positives = 27/45 (60%), Gaps = 0/45 (0%)

Query  12   IAAQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            +  QR+LI   +   D+VI +A   G+  P LI+++ +R MK GS
Sbjct  827  LKKQRDLIEKYLCISDIVITSACKPGEECPILITKEAVRKMKSGS  871


> dre:100331743  Nicotinamide Nucleotide Transhydrogenase family 
member (nnt-1)-like
Length=518

 Score = 34.7 bits (78),  Expect = 0.077, Method: Composition-based stats.
 Identities = 16/39 (41%), Positives = 24/39 (61%), Gaps = 0/39 (0%)

Query  18  LIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
           L A   +  D++I TA   GK +P LI R+M+ +M+ GS
Sbjct  3   LFAKQCRDVDIIISTALNPGKRAPVLIKREMVESMRDGS  41


> tgo:TGME49_115260  alanine dehydrogenase, putative (EC:1.4.1.1)
Length=390

 Score = 34.3 bits (77),  Expect = 0.095, Method: Composition-based stats.
 Identities = 12/31 (38%), Positives = 22/31 (70%), Gaps = 0/31 (0%)

Query  26   CDVVICTAAIHGKPSPKLISRDMLRTMKPGS  56
            CD+++    + G+ +PK++ R+ L+ MKPGS
Sbjct  231  CDLLVGAVLLPGRKAPKIVRREHLKRMKPGS  261


> mmu:71664  Mettl7b, 0610006F02Rik, AI266817; methyltransferase 
like 7B
Length=244

 Score = 28.1 bits (61),  Expect = 6.3, Method: Composition-based stats.
 Identities = 10/29 (34%), Positives = 19/29 (65%), Gaps = 0/29 (0%)

Query  27   DVVICTAAIHGKPSPKLISRDMLRTMKPG  55
            DVV+CT  +    SP+ + +++ R ++PG
Sbjct  139  DVVVCTLVLCSVQSPRKVLQEVQRVLRPG  167


> mmu:70152  Mettl7a1, 2210414H16Rik, 3300001H21Rik, Aam-B, Mettl7a, 
UbiE1; methyltransferase like 7A1
Length=244

 Score = 28.1 bits (61),  Expect = 6.4, Method: Composition-based stats.
 Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 0/44 (0%)

Query  12   IAAQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPG  55
            +AA  ++   T    DVV+CT  +    + + I R++ R +KPG
Sbjct  124  VAAGEDMHQVTDGSVDVVVCTLVLCSVKNQEKILREVCRVLKPG  167


> mmu:393082  Mettl7a2, Gm921, UbiE2, Ubie; methyltransferase like 
7A2
Length=244

 Score = 28.1 bits (61),  Expect = 6.4, Method: Composition-based stats.
 Identities = 14/44 (31%), Positives = 24/44 (54%), Gaps = 0/44 (0%)

Query  12   IAAQRELIANTIKHCDVVICTAAIHGKPSPKLISRDMLRTMKPG  55
            +AA  ++   T    DVV+CT  +    + + I R++ R +KPG
Sbjct  124  VAAGEDMHQVTDGSVDVVVCTLVLCSVKNQEKILREVCRVLKPG  167


> hsa:196410  METTL7B, MGC17301; methyltransferase like 7B
Length=244

 Score = 28.1 bits (61),  Expect = 7.9, Method: Composition-based stats.
 Identities = 10/29 (34%), Positives = 19/29 (65%), Gaps = 0/29 (0%)

Query  27   DVVICTAAIHGKPSPKLISRDMLRTMKPG  55
            DVV+CT  +    SP+ + +++ R ++PG
Sbjct  139  DVVVCTLVLCSVQSPRKVLQEVRRVLRPG  167



Lambda     K      H
   0.324    0.132    0.408 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 

Effective search space used: 2005549208


  Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
    Posted date:  Sep 17, 2011 11:19 AM
  Number of letters in database: 82,071,388
  Number of sequences in database:  164,496



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40