bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.24+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
164,496 sequences; 82,071,388 total letters
Query= Emax_0143_orf1
Length=144
Score E
Sequences producing significant alignments: (Bits) Value
tgo:TGME49_034190 glycine hydroxymethyltransferase, putative (... 218 6e-57
ath:AT4G13930 SHM4; SHM4 (serine hydroxymethyltransferase 4); ... 200 1e-51
ath:AT4G32520 SHM3; SHM3 (SERINE HYDROXYMETHYLTRANSFERASE 3); ... 198 4e-51
mmu:108037 Shmt2, 2700043D08Rik, AA408223, AA986903; serine hy... 198 6e-51
xla:447193 shmt2, MGC79128; serine hydroxymethyltransferase 2 ... 196 2e-50
cel:C05D11.11 mel-32; Maternal Effect Lethal family member (me... 195 4e-50
sce:YLR058C SHM2, SHMT2; Cytosolic serine hydroxymethyltransfe... 194 7e-50
dre:394021 shmt1, MGC66171, zgc:66171, zgc:77524; serine hydro... 192 3e-49
ath:AT4G13890 EDA36; EDA36 (EMBRYO SAC DEVELOPMENT ARREST 37);... 192 3e-49
ath:AT4G37930 SHM1; SHM1 (SERINE TRANSHYDROXYMETHYLTRANSFERASE... 190 2e-48
dre:100144628 shmt2; serine hydroxymethyltransferase 2 (mitoch... 188 4e-48
xla:380048 shmt1, MGC53442; serine hydroxymethyltransferase 1 ... 188 5e-48
ath:AT5G26780 SHM2; SHM2 (SERINE HYDROXYMETHYLTRANSFERASE 2); ... 186 2e-47
tpv:TP01_0390 serine hydroxymethyltransferase; K00600 glycine ... 186 2e-47
cpv:cgd8_2610 cytosolic serine hydroxymethyl transferase ; K00... 182 3e-46
hsa:6470 SHMT1, CSHMT, MGC15229, MGC24556, SHMT; serine hydrox... 181 5e-46
mmu:20425 Shmt1, AI324848, AI385541, C81125, Shmt, mshmt, mshm... 180 2e-45
bbo:BBOV_IV005840 23.m06426; serine hydroxymethyltransferase (... 177 8e-45
ath:AT1G36370 SHM7; SHM7 (serine hydroxymethyltransferase 7); ... 173 2e-43
hsa:6472 SHMT2, GLYA, SHMT; serine hydroxymethyltransferase 2 ... 170 1e-42
ath:AT1G22020 SHM6; SHM6 (serine hydroxymethyltransferase 6); ... 169 2e-42
sce:YBR263W SHM1, SHMT1, TMP3; Mitochondrial serine hydroxymet... 157 9e-39
pfa:PFL1720w serine hydroxymethyltransferase (EC:2.1.2.1); K00... 146 2e-35
eco:b2551 glyA, ECK2548, JW2535; serine hydroxymethyltransfera... 127 1e-29
cpv:cgd8_2580 mitochondrial serine hydroxymethyl transferase 42.7 4e-04
tpv:TP01_1094 cysteine desulfurase 33.1 0.34
tgo:TGME49_005560 nascent polypeptide-associated complex alpha... 32.7 0.39
pfa:PF14_0534 serine hydroxymethyltransferase, putative (EC:2.... 31.6 0.93
pfa:PFE0485w phosphatidylinositol 4-kinase, putative (EC:2.7.1... 30.4 1.8
cpv:cgd8_3240 apicomplexan specific protein 30.4 1.9
mmu:224613 Flywch1, E030034P13Rik, mKIAA1552; FLYWCH-type zinc... 29.6 3.1
sce:YML124C TUB3; Alpha-tubulin; associates with beta-tubulin ... 29.6 3.3
ath:AT2G29940 PDR3; PDR3 (PLEIOTROPIC DRUG RESISTANCE 3); ATPa... 29.6 3.7
> tgo:TGME49_034190 glycine hydroxymethyltransferase, putative
(EC:2.1.2.1); K00600 glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=595
Score = 218 bits (554), Expect = 6e-57, Method: Compositional matrix adjust.
Identities = 102/143 (71%), Positives = 116/143 (81%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQP SGSPAN AVF+ LLQPHDR MGL L GGHLTHG YT +RISA+S+FFESLPY
Sbjct 241 VNVQPYSGSPANMAVFVGLLQPHDRIMGLDLPSGGHLTHGFYTAKKRISATSIFFESLPY 300
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
G++ +TGLIDYEEL + A +FRPKLIICGHSAYPR L+Y +FR IAD GA L CDMAH
Sbjct 301 GVDEKTGLIDYEELRKRALVFRPKLIICGHSAYPRDLDYVKFREIADAAGAMLMCDMAHT 360
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG +AA + SPF YCD+VTTTT
Sbjct 361 SGLIAANLLTSPFPYCDIVTTTT 383
> ath:AT4G13930 SHM4; SHM4 (serine hydroxymethyltransferase 4);
catalytic/ glycine hydroxymethyltransferase/ pyridoxal phosphate
binding (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=471
Score = 200 bits (508), Expect = 1e-51, Method: Compositional matrix adjust.
Identities = 98/144 (68%), Positives = 113/144 (78%), Gaps = 1/144 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPS-RRISASSLFFESLP 60
VNVQP SGSPANFA + ALLQPHDR MGL L GGHLTHG YT ++ISA+S++FESLP
Sbjct 99 VNVQPYSGSPANFAAYTALLQPHDRIMGLDLPSGGHLTHGYYTSGGKKISATSIYFESLP 158
Query 61 YGLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAH 120
Y +N TG IDY++L+ A FRPKL+ICG SAYPR +Y RFR+IADKVGA L CDMAH
Sbjct 159 YKVNFTTGYIDYDKLEEKALDFRPKLLICGGSAYPRDWDYARFRAIADKVGALLLCDMAH 218
Query 121 FSGFVAAGIFESPFDYCDVVTTTT 144
SG VAA +PF+YCDVVTTTT
Sbjct 219 ISGLVAAQEAANPFEYCDVVTTTT 242
> ath:AT4G32520 SHM3; SHM3 (SERINE HYDROXYMETHYLTRANSFERASE 3);
catalytic/ glycine hydroxymethyltransferase/ pyridoxal phosphate
binding (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=529
Score = 198 bits (504), Expect = 4e-51, Method: Compositional matrix adjust.
Identities = 92/143 (64%), Positives = 110/143 (76%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQPLSGSPANFAV+ A+L PHDR MGL L GGHL+HG T RR+S +S++FES+PY
Sbjct 170 VNVQPLSGSPANFAVYTAILSPHDRIMGLDLPHGGHLSHGFMTAKRRVSGTSIYFESMPY 229
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
L+ TG++DY+ L++ ATLFRPKLII G SAY R +Y R R IAD VGAFL DMAH
Sbjct 230 RLDESTGIVDYDMLEKTATLFRPKLIIAGASAYSRDFDYPRMRKIADSVGAFLMMDMAHI 289
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAA + PF+YCD+VTTTT
Sbjct 290 SGLVAASVVADPFEYCDIVTTTT 312
> mmu:108037 Shmt2, 2700043D08Rik, AA408223, AA986903; serine
hydroxymethyltransferase 2 (mitochondrial) (EC:2.1.2.1); K00600
glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=504
Score = 198 bits (503), Expect = 6e-51, Method: Compositional matrix adjust.
Identities = 95/143 (66%), Positives = 109/143 (76%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQP SGSPAN A + ALLQPHDR MGL L DGGHLTHG + +RISA+S+FFES+PY
Sbjct 136 VNVQPYSGSPANLAAYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPY 195
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
LNPQTGLIDY++L A LFRP+LII G SAY RL++Y R R + D+V A L DMAH
Sbjct 196 KLNPQTGLIDYDQLALTARLFRPRLIIAGTSAYARLIDYARMREVCDEVRAHLLADMAHI 255
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAA + SPF Y DVVTTTT
Sbjct 256 SGLVAAKVIPSPFKYADVVTTTT 278
> xla:447193 shmt2, MGC79128; serine hydroxymethyltransferase
2 (mitochondrial) (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=496
Score = 196 bits (498), Expect = 2e-50, Method: Compositional matrix adjust.
Identities = 90/143 (62%), Positives = 112/143 (78%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQP SGSPANFA + A+LQPHDR MGL L DGGHLTHG + +RISA+S++FES+PY
Sbjct 128 VNVQPYSGSPANFAAYTAVLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIYFESMPY 187
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
LNP TGLI+Y++L+ A LFRPKLII G SAY RL++Y + R + D+V A+L DMAH
Sbjct 188 KLNPATGLINYDQLEMTARLFRPKLIIAGTSAYARLIDYAKMRKVCDEVKAYLLADMAHI 247
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAAG+ SPF + D+VT+TT
Sbjct 248 SGLVAAGVIPSPFQHADIVTSTT 270
> cel:C05D11.11 mel-32; Maternal Effect Lethal family member (mel-32);
K00600 glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=507
Score = 195 bits (495), Expect = 4e-50, Method: Compositional matrix adjust.
Identities = 93/143 (65%), Positives = 114/143 (79%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQPLSGSPANFAV+ A++ + R MGL L DGGHLTHG +TP+R++SA+S FF+SLPY
Sbjct 139 VNVQPLSGSPANFAVYTAIVGSNGRIMGLDLPDGGHLTHGFFTPARKVSATSEFFQSLPY 198
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
++P TGLIDY++L++ A LFRPK II G S Y R L+Y+RFR IA K GA+L DMAH
Sbjct 199 KVDPTTGLIDYDKLEQNAMLFRPKAIIAGVSCYARHLDYERFRKIATKAGAYLMSDMAHI 258
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAAG+ SPF+Y DVVTTTT
Sbjct 259 SGLVAAGLIPSPFEYSDVVTTTT 281
> sce:YLR058C SHM2, SHMT2; Cytosolic serine hydroxymethyltransferase,
converts serine to glycine plus 5,10 methylenetetrahydrofolate;
major isoform involved in generating precursors
for purine, pyrimidine, amino acid, and lipid biosynthesis (EC:2.1.2.1);
K00600 glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=469
Score = 194 bits (494), Expect = 7e-50, Method: Compositional matrix adjust.
Identities = 87/143 (60%), Positives = 113/143 (79%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQ LSGSPAN V+ A+++PH+R MGL L DGGHL+HG T +R+ISA S +FES PY
Sbjct 104 VNVQTLSGSPANLQVYQAIMKPHERLMGLYLPDGGHLSHGYATENRKISAVSTYFESFPY 163
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
+NP+TG+IDY+ L++ A L+RPK+++ G SAY RL++YKR R IADK GA+L DMAH
Sbjct 164 RVNPETGIIDYDTLEKNAILYRPKVLVAGTSAYCRLIDYKRMREIADKCGAYLMVDMAHI 223
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG +AAG+ SPF+Y D+VTTTT
Sbjct 224 SGLIAAGVIPSPFEYADIVTTTT 246
> dre:394021 shmt1, MGC66171, zgc:66171, zgc:77524; serine hydroxymethyltransferase
1 (soluble) (EC:2.1.2.1); K00600 glycine
hydroxymethyltransferase [EC:2.1.2.1]
Length=481
Score = 192 bits (488), Expect = 3e-49, Method: Compositional matrix adjust.
Identities = 90/143 (62%), Positives = 109/143 (76%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQP SGS ANFAV+ A+++PH R MGL L DGGHLTHG T ++ISA+S+FFES+PY
Sbjct 110 VNVQPYSGSRANFAVYTAIVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPY 169
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
+NP+TG IDY L+ A LF P+LII G S Y R L+Y R R IAD+ GA+L DMAH
Sbjct 170 KVNPETGYIDYNRLEENARLFHPRLIIAGTSCYSRNLDYSRLRKIADENGAYLLADMAHI 229
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAAG+ SPF+YCDVV+TTT
Sbjct 230 SGLVAAGVVPSPFEYCDVVSTTT 252
> ath:AT4G13890 EDA36; EDA36 (EMBRYO SAC DEVELOPMENT ARREST 37);
catalytic/ glycine hydroxymethyltransferase/ pyridoxal phosphate
binding (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=470
Score = 192 bits (488), Expect = 3e-49, Method: Compositional matrix adjust.
Identities = 91/144 (63%), Positives = 112/144 (77%), Gaps = 1/144 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPS-RRISASSLFFESLP 60
VNVQP SGSPANFA + ALLQPHDR MGL L GGH+THG Y+ + ISA+S++FE+LP
Sbjct 99 VNVQPYSGSPANFAAYTALLQPHDRIMGLDLPSGGHITHGYYSSGGKNISATSIYFENLP 158
Query 61 YGLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAH 120
Y ++ +TG IDY++L+ A FRPKLIICG ++YPR +Y RFR++ADKVGAFL CDMAH
Sbjct 159 YKVDSKTGYIDYDKLEEKAMDFRPKLIICGGTSYPREWDYARFRAVADKVGAFLLCDMAH 218
Query 121 FSGFVAAGIFESPFDYCDVVTTTT 144
S VAA PF+YCDVVTT+T
Sbjct 219 NSALVAAQEAADPFEYCDVVTTST 242
> ath:AT4G37930 SHM1; SHM1 (SERINE TRANSHYDROXYMETHYLTRANSFERASE
1); glycine hydroxymethyltransferase/ poly(U) binding (EC:2.1.2.1);
K00600 glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=517
Score = 190 bits (482), Expect = 2e-48, Method: Composition-based stats.
Identities = 86/143 (60%), Positives = 108/143 (75%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQPLSGSPANF V+ ALL+PH+R M L L GGHL+HG T +++ISA S+FFE++PY
Sbjct 142 VNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPY 201
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
L+ TG IDY+++++ ATLFRPKLI+ G SAY RL +Y R R + +K A + DMAH
Sbjct 202 RLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYARIRKVCNKQKAVMLADMAHI 261
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAA + SPFDY DVVTTTT
Sbjct 262 SGLVAANVIPSPFDYADVVTTTT 284
> dre:100144628 shmt2; serine hydroxymethyltransferase 2 (mitochondrial)
(EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=492
Score = 188 bits (478), Expect = 4e-48, Method: Compositional matrix adjust.
Identities = 86/143 (60%), Positives = 110/143 (76%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQP SGSPANFA + A+L PH+R MGL L DGGHLTHG + RRISA+S++FES+PY
Sbjct 125 VNVQPYSGSPANFAAYTAVLNPHERIMGLDLPDGGHLTHGYMSDVRRISATSIYFESMPY 184
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
LNP+TGLIDY++++ A LFRPKLII G SAY RL++Y R +++ ++ A++ DMAH
Sbjct 185 KLNPKTGLIDYDQMELTAKLFRPKLIIAGTSAYARLIDYCRIKTLCSEINAYMLADMAHI 244
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAA SPF + D+VTTTT
Sbjct 245 SGLVAAKAIPSPFQHADIVTTTT 267
> xla:380048 shmt1, MGC53442; serine hydroxymethyltransferase
1 (soluble) (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=485
Score = 188 bits (477), Expect = 5e-48, Method: Compositional matrix adjust.
Identities = 88/143 (61%), Positives = 109/143 (76%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQP SGSPANFA++ AL++PH R MGL L DGGHLTHG T ++ISA+S+FFES+PY
Sbjct 114 VNVQPYSGSPANFAIYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPY 173
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
++P+TG IDY+ L+ A LF PK+II G S Y R L+Y R R IAD+ A L DMAH
Sbjct 174 KVHPETGYIDYDRLEENARLFHPKMIIAGVSCYSRNLDYARMRRIADENNAVLMADMAHI 233
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAAG+ SPF++CDVV+TTT
Sbjct 234 SGLVAAGVVPSPFEHCDVVSTTT 256
> ath:AT5G26780 SHM2; SHM2 (SERINE HYDROXYMETHYLTRANSFERASE 2);
catalytic/ glycine hydroxymethyltransferase/ pyridoxal phosphate
binding (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=533
Score = 186 bits (473), Expect = 2e-47, Method: Composition-based stats.
Identities = 85/143 (59%), Positives = 107/143 (74%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQ LSGSPANF V+ ALL+PH+R M L L GGHL+HG T +++ISA S+FFE++PY
Sbjct 142 VNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETMPY 201
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
L+ TG IDY++L++ A LFRPKLI+ G SAY RL +Y R R + +K A + DMAH
Sbjct 202 RLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDYARIRKVCNKQKAVMLADMAHI 261
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAAG+ SPF+Y DVVTTTT
Sbjct 262 SGLVAAGVIPSPFEYADVVTTTT 284
> tpv:TP01_0390 serine hydroxymethyltransferase; K00600 glycine
hydroxymethyltransferase [EC:2.1.2.1]
Length=503
Score = 186 bits (473), Expect = 2e-47, Method: Compositional matrix adjust.
Identities = 89/143 (62%), Positives = 107/143 (74%), Gaps = 0/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQPLSGSPAN AV+ ALLQPHD+ MGL L GGHLTHG Y +++SASS+FF L Y
Sbjct 153 VNVQPLSGSPANLAVYCALLQPHDKLMGLSLESGGHLTHGYYNAKKKVSASSIFFSPLSY 212
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
L+P+TGLIDY+ L++ A F PKLII G S Y R ++YKRFR IAD VGA+L D+AH
Sbjct 213 FLDPKTGLIDYDGLEKSAQAFCPKLIIAGASTYSRYIDYKRFREIADSVGAYLMADIAHI 272
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VA + PF+YC VVT+TT
Sbjct 273 SGLVAGRVHPLPFEYCHVVTSTT 295
> cpv:cgd8_2610 cytosolic serine hydroxymethyl transferase ; K00600
glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=445
Score = 182 bits (462), Expect = 3e-46, Method: Composition-based stats.
Identities = 84/142 (59%), Positives = 107/142 (75%), Gaps = 1/142 (0%)
Query 3 NVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPYG 62
NV+P SGSPANFAV A+L+P+DR MGL L GGHLTHG YT +R++ SS +FESLPY
Sbjct 93 NVKPHSGSPANFAVLNAVLKPNDRIMGLSLQHGGHLTHGHYTNLKRVNCSSHYFESLPY- 151
Query 63 LNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHFS 122
+ G+IDY++L+ A LFRPK+II G S YPR++N+KRFR I DKV A+L D+AH+S
Sbjct 152 VTDLEGVIDYDKLEENAILFRPKMIIAGASGYPRMINFKRFRDICDKVKAYLMVDIAHYS 211
Query 123 GFVAAGIFESPFDYCDVVTTTT 144
G V AG + SP DY D +TTT+
Sbjct 212 GLVVAGKYPSPKDYADFITTTS 233
> hsa:6470 SHMT1, CSHMT, MGC15229, MGC24556, SHMT; serine hydroxymethyltransferase
1 (soluble) (EC:2.1.2.1); K00600 glycine
hydroxymethyltransferase [EC:2.1.2.1]
Length=483
Score = 181 bits (460), Expect = 5e-46, Method: Compositional matrix adjust.
Identities = 85/136 (62%), Positives = 103/136 (75%), Gaps = 0/136 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQP SGSPANFAV+ AL++PH R MGL L DGGHLTHG T ++ISA+S+FFES+PY
Sbjct 113 VNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPY 172
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
+NP TG I+Y++L+ A LF PKLII G S Y R L Y R R IAD+ GA+L DMAH
Sbjct 173 KVNPDTGYINYDQLEENARLFHPKLIIAGTSCYSRNLEYARLRKIADENGAYLMADMAHI 232
Query 122 SGFVAAGIFESPFDYC 137
SG VAAG+ SPF++C
Sbjct 233 SGLVAAGVVPSPFEHC 248
> mmu:20425 Shmt1, AI324848, AI385541, C81125, Shmt, mshmt, mshmt1,
mshmt2; serine hydroxymethyltransferase 1 (soluble) (EC:2.1.2.1);
K00600 glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=478
Score = 180 bits (456), Expect = 2e-45, Method: Compositional matrix adjust.
Identities = 84/136 (61%), Positives = 104/136 (76%), Gaps = 0/136 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQP SGSPANFAV+ AL++PH R MGL L DGGHLTHG T ++ISA+S+FFES+PY
Sbjct 107 VNVQPYSGSPANFAVYTALVEPHGRIMGLDLPDGGHLTHGFMTDKKKISATSIFFESMPY 166
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
+ P+TG I+Y++L+ A+LF PKLII G S Y R L+Y R R IAD GA+L DMAH
Sbjct 167 KVYPETGYINYDQLEENASLFHPKLIIAGTSCYSRNLDYARLRKIADDNGAYLMADMAHI 226
Query 122 SGFVAAGIFESPFDYC 137
SG VAAG+ SPF++C
Sbjct 227 SGLVAAGVVPSPFEHC 242
> bbo:BBOV_IV005840 23.m06426; serine hydroxymethyltransferase
(EC:2.1.2.1); K00600 glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=453
Score = 177 bits (450), Expect = 8e-45, Method: Composition-based stats.
Identities = 79/136 (58%), Positives = 101/136 (74%), Gaps = 0/136 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQPLSGSPAN V+M LLQPHD+ MGL L GGHLTHG Y ++ISA+++F+ SL Y
Sbjct 103 VNVQPLSGSPANLEVYMGLLQPHDKIMGLRLASGGHLTHGFYVGQKKISATAVFYTSLQY 162
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
+N +TGL+DY++++RLA + PKLII G S Y R +YKR R IADKVGA+L D+AH
Sbjct 163 DVNKETGLLDYDDMERLAKAYCPKLIIAGASCYSRYWDYKRCREIADKVGAYLMADIAHI 222
Query 122 SGFVAAGIFESPFDYC 137
+G +A SPF+YC
Sbjct 223 AGLIAGEAHPSPFEYC 238
> ath:AT1G36370 SHM7; SHM7 (serine hydroxymethyltransferase 7);
catalytic/ glycine hydroxymethyltransferase/ pyridoxal phosphate
binding (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=598
Score = 173 bits (438), Expect = 2e-43, Method: Compositional matrix adjust.
Identities = 82/144 (56%), Positives = 105/144 (72%), Gaps = 1/144 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPS-RRISASSLFFESLP 60
VNVQP S + ANFAV+ LL P +R MGL GGH++HG TP ++ISA+S+FFES P
Sbjct 225 VNVQPYSCTSANFAVYTGLLLPGERIMGLDSPSGGHMSHGYCTPGGKKISAASIFFESFP 284
Query 61 YGLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAH 120
Y +NPQTG IDY++L+ A +RPK++ICG S+YPR ++ R R IADK GA L CDMAH
Sbjct 285 YKVNPQTGYIDYDKLEDKALDYRPKILICGGSSYPRDWDFARVRQIADKCGAVLMCDMAH 344
Query 121 FSGFVAAGIFESPFDYCDVVTTTT 144
SG VA +PFD+CD+VT+TT
Sbjct 345 ISGLVATKECSNPFDHCDIVTSTT 368
> hsa:6472 SHMT2, GLYA, SHMT; serine hydroxymethyltransferase
2 (mitochondrial) (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=494
Score = 170 bits (431), Expect = 1e-42, Method: Compositional matrix adjust.
Identities = 86/143 (60%), Positives = 100/143 (69%), Gaps = 10/143 (6%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQP SGSPAN AV+ ALLQPHDR MGL L DGGHLTHG + +RISA+S+FFES+PY
Sbjct 136 VNVQPYSGSPANLAVYTALLQPHDRIMGLDLPDGGHLTHGYMSDVKRISATSIFFESMPY 195
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
LN L A LFRP+LII G SAY RL++Y R R + D+V A L DMAH
Sbjct 196 KLN----------LALTARLFRPRLIIAGTSAYARLIDYARMREVCDEVKAHLLADMAHI 245
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
SG VAA + SPF + D+VTTTT
Sbjct 246 SGLVAAKVIPSPFKHADIVTTTT 268
> ath:AT1G22020 SHM6; SHM6 (serine hydroxymethyltransferase 6);
catalytic/ glycine hydroxymethyltransferase/ pyridoxal phosphate
binding (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=599
Score = 169 bits (429), Expect = 2e-42, Method: Compositional matrix adjust.
Identities = 81/144 (56%), Positives = 104/144 (72%), Gaps = 1/144 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPS-RRISASSLFFESLP 60
VNVQP S + ANFAVF LL P +R MGL GGH++HG YTP +++S +S+FFES P
Sbjct 229 VNVQPYSCTSANFAVFTGLLMPGERIMGLDSPSGGHMSHGYYTPGGKKVSGASIFFESFP 288
Query 61 YGLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAH 120
Y ++P+TG IDY++L+ A +RPK++ICG S+YPR + RFR IADK GA L DMA
Sbjct 289 YKVDPRTGYIDYDKLEEKALDYRPKILICGGSSYPRDWEFPRFRHIADKCGAVLMFDMAQ 348
Query 121 FSGFVAAGIFESPFDYCDVVTTTT 144
SG VAA +PFDYCD+VT+TT
Sbjct 349 ISGLVAAKESPNPFDYCDIVTSTT 372
> sce:YBR263W SHM1, SHMT1, TMP3; Mitochondrial serine hydroxymethyltransferase,
converts serine to glycine plus 5,10 methylenetetrahydrofolate;
involved in generating precursors for
purine, pyrimidine, amino acid, and lipid biosynthesis; reverse
reaction generates serine (EC:2.1.2.1); K00600 glycine hydroxymethyltransferase
[EC:2.1.2.1]
Length=490
Score = 157 bits (398), Expect = 9e-39, Method: Compositional matrix adjust.
Identities = 78/144 (54%), Positives = 101/144 (70%), Gaps = 1/144 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRR-ISASSLFFESLP 60
VNVQPLSG+PAN V+ A++ +R MGL L DGGHL+HG S IS S +F+S+P
Sbjct 120 VNVQPLSGAPANLYVYSAIMNVGERLMGLDLPDGGHLSHGYQLKSGTPISFISKYFQSMP 179
Query 61 YGLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAH 120
Y ++ TGLIDY+ L LA FRPK+I+ G SAY RL++Y RF+ I+ GA+L DMAH
Sbjct 180 YHVDHTTGLIDYDNLQVLAKAFRPKVIVAGTSAYSRLIDYARFKEISQGCGAYLMSDMAH 239
Query 121 FSGFVAAGIFESPFDYCDVVTTTT 144
SG VAA + SPF++ D+VTTTT
Sbjct 240 ISGLVAANVVPSPFEHSDIVTTTT 263
> pfa:PFL1720w serine hydroxymethyltransferase (EC:2.1.2.1); K00600
glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=442
Score = 146 bits (368), Expect = 2e-35, Method: Composition-based stats.
Identities = 70/143 (48%), Positives = 95/143 (66%), Gaps = 1/143 (0%)
Query 2 VNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPY 61
VNVQPLSGS AN AL+ + MG+ L GGHLTHG + +++S +S FES Y
Sbjct 94 VNVQPLSGSAANVQALYALVGVKGKIMGMHLCSGGHLTHGFFDEKKKVSITSDLFESKLY 153
Query 62 GLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHF 121
N + G +D E + LA F+PK+IICG+++YPR ++YK FR I D+V A+L+ D++H
Sbjct 154 KCNSE-GYVDMESVRNLALSFQPKVIICGYTSYPRDIDYKGFREICDEVNAYLFADISHI 212
Query 122 SGFVAAGIFESPFDYCDVVTTTT 144
S FVA + +PF Y DVVTTTT
Sbjct 213 SSFVACNLLNNPFTYADVVTTTT 235
> eco:b2551 glyA, ECK2548, JW2535; serine hydroxymethyltransferase
(EC:2.1.2.1); K00600 glycine hydroxymethyltransferase [EC:2.1.2.1]
Length=417
Score = 127 bits (319), Expect = 1e-29, Method: Compositional matrix adjust.
Identities = 62/134 (46%), Positives = 91/134 (67%), Gaps = 6/134 (4%)
Query 1 HVNVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLP 60
+ NVQP SGS ANFAV+ ALL+P D +G+ L GGHLTHG+ ++ S + +P
Sbjct 90 YANVQPHSGSQANFAVYTALLEPGDTVLGMNLAHGGHLTHGS-----PVNFSGKLYNIVP 144
Query 61 YGLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAH 120
YG++ TG IDY +L++ A +PK+II G SAY ++++ + R IAD +GA+L+ DMAH
Sbjct 145 YGIDA-TGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAH 203
Query 121 FSGFVAAGIFESPF 134
+G VAAG++ +P
Sbjct 204 VAGLVAAGVYPNPV 217
> cpv:cgd8_2580 mitochondrial serine hydroxymethyl transferase
Length=438
Score = 42.7 bits (99), Expect = 4e-04, Method: Composition-based stats.
Identities = 30/146 (20%), Positives = 62/146 (42%), Gaps = 18/146 (12%)
Query 3 NVQPLSGSPANFAVFMALLQPHDRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPYG 62
N+Q SGS A A+ M LL DR + + + L + +++ Y
Sbjct 95 NIQCYSGSHAELAICMGLLNKGDRILRIRGDSDTVLEN--------------YYQVEYYN 140
Query 63 LNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWCDMAHFS 122
L+ + D +L + +PKL++ ++Y+ I ++ FL D++ +
Sbjct 141 LDKKGRGFDIVDLREKCKILKPKLLLVPSDVLTLFIDYRLLSEICNEFKIFLVADISEIA 200
Query 123 GFVAAGIF----ESPFDYCDVVTTTT 144
++ + +P+ YCD++ + T
Sbjct 201 LLISFDRYGREDNNPYRYCDIIYSNT 226
> tpv:TP01_1094 cysteine desulfurase
Length=469
Score = 33.1 bits (74), Expect = 0.34, Method: Compositional matrix adjust.
Identities = 21/81 (25%), Positives = 35/81 (43%), Gaps = 2/81 (2%)
Query 58 SLPYGLNPQTGLIDYEELDRLATLFRPKLIICGHSA--YPRLLNYKRFRSIADKVGAFLW 115
S+ Y Q G D + L+ L PKL+ CGH++ + + K +A K G +
Sbjct 200 SIEYVKLHQNGQFDLDHLESLLKSKSPKLLCCGHASNVLGVIQDMKTISKLAHKYGCLVL 259
Query 116 CDMAHFSGFVAAGIFESPFDY 136
D A G + + + D+
Sbjct 260 SDSAQTVGKIKIDVQDMDVDF 280
> tgo:TGME49_005560 nascent polypeptide-associated complex alpha
chain, putative (EC:3.4.21.68 3.6.3.8); K03626 nascent polypeptide-associated
complex subunit alpha
Length=6052
Score = 32.7 bits (73), Expect = 0.39, Method: Compositional matrix adjust.
Identities = 21/64 (32%), Positives = 28/64 (43%), Gaps = 7/64 (10%)
Query 33 NDGGHLTHGAYTPSRRISASSLFFESLPYGLNPQTGLIDYEELDRLAT------LFRPKL 86
GG G RR+ +SLFF LP G++P E RL T L R +
Sbjct 3989 EQGGKADRG-NAEDRRVEDASLFFSPLPLGVSPPARETPEERQARLLTAAIVELLNRRRK 4047
Query 87 IICG 90
++CG
Sbjct 4048 VLCG 4051
> pfa:PF14_0534 serine hydroxymethyltransferase, putative (EC:2.1.2.1)
Length=462
Score = 31.6 bits (70), Expect = 0.93, Method: Composition-based stats.
Identities = 17/78 (21%), Positives = 37/78 (47%), Gaps = 6/78 (7%)
Query 71 DYEELDRLATLFRPKLIICGHSAYPRLLNYKRF------RSIADKVGAFLWCDMAHFSGF 124
+Y+E+ +++ F P +I S P ++Y RF ++ + +M + +
Sbjct 168 NYDEIQKISNDFNPDIIYFDESNNPYNIDYDRFIKGLKNKNNKIHNKPIIITNMNNKANL 227
Query 125 VAAGIFESPFDYCDVVTT 142
++ + SPF + D+V T
Sbjct 228 ISQNLINSPFTHSDIVFT 245
> pfa:PFE0485w phosphatidylinositol 4-kinase, putative (EC:2.7.1.67);
K00888 phosphatidylinositol 4-kinase [EC:2.7.1.67]
Length=1559
Score = 30.4 bits (67), Expect = 1.8, Method: Composition-based stats.
Identities = 28/83 (33%), Positives = 42/83 (50%), Gaps = 4/83 (4%)
Query 10 SPANFAVFMALLQPHDRFMG-LGLNDGGHLTHGAYTPSRRISASSLFFESLPYGLNPQ-T 67
S A +++ LLQ DR G L L+ GHL H Y S ++ FE+ P+ L +
Sbjct 1397 SHAAYSLISYLLQVKDRHNGNLLLDSDGHLIHIDYGFMLTNSPGNVNFETSPFKLTQEYL 1456
Query 68 GLIDYEELDRLATLFRPKLIICG 90
++D E+ D FR +LI+ G
Sbjct 1457 DIMDGEKSDNY-EYFR-RLIVSG 1477
> cpv:cgd8_3240 apicomplexan specific protein
Length=749
Score = 30.4 bits (67), Expect = 1.9, Method: Composition-based stats.
Identities = 19/74 (25%), Positives = 30/74 (40%), Gaps = 9/74 (12%)
Query 71 DYEELDRLATLFRPKLIICGHSAYPRLLNYKRFRSIADKVGAFLWC---------DMAHF 121
+Y E + + K I H+A ++N + R +V F WC DM F
Sbjct 17 NYNEDKKDGKIAAKKQIELEHNAAKAIMNLRHRRDCNGEVTRFSWCSDPLCRMWYDMTQF 76
Query 122 SGFVAAGIFESPFD 135
GF+ I ++ D
Sbjct 77 FGFLGEDIIDTDID 90
> mmu:224613 Flywch1, E030034P13Rik, mKIAA1552; FLYWCH-type zinc
finger 1
Length=673
Score = 29.6 bits (65), Expect = 3.1, Method: Composition-based stats.
Identities = 19/67 (28%), Positives = 31/67 (46%), Gaps = 6/67 (8%)
Query 44 TPSRRISASSLFFESLPYGLNPQTGLIDYEELDRLATLFRPKLIICGHSAYPRLLNYKRF 103
TP SL +SLP P G++ Y L+ L T + ++ H ++ YKR
Sbjct 202 TPEEEQGYRSLALQSLPPKKRPTPGVVRYRPLEFLKTCYGGTFLV--HQSFL----YKRE 255
Query 104 RSIADKV 110
+++ KV
Sbjct 256 KTVGGKV 262
> sce:YML124C TUB3; Alpha-tubulin; associates with beta-tubulin
(Tub2p) to form tubulin dimer, which polymerizes to form microtubules;
expressed at lower level than Tub1p
Length=445
Score = 29.6 bits (65), Expect = 3.3, Method: Composition-based stats.
Identities = 26/92 (28%), Positives = 37/92 (40%), Gaps = 13/92 (14%)
Query 31 GLNDGGHLTHGAYTPSRRISASSLFFESLPYG--------LNPQTGLIDYEELDRLATLF 82
G+ + GHL G P S FF YG ++ + +ID R LF
Sbjct 29 GIKEDGHLEDGLSKPKGGEEGFSTFFHETGYGKFVPRAIYVDLEPNVIDEVRTGRFKELF 88
Query 83 RPKLIICGH----SAYPRLLNYKRFRSIADKV 110
P+ +I G + Y R +Y R I D+V
Sbjct 89 HPEQLINGKEDAANNYAR-GHYTVGREIVDEV 119
> ath:AT2G29940 PDR3; PDR3 (PLEIOTROPIC DRUG RESISTANCE 3); ATPase,
coupled to transmembrane movement of substances
Length=1426
Score = 29.6 bits (65), Expect = 3.7, Method: Compositional matrix adjust.
Identities = 23/61 (37%), Positives = 31/61 (50%), Gaps = 9/61 (14%)
Query 16 VFMALLQPH----DRFMGLGLNDGGHLTHGAYTPSRRISASSLFFESLPYGLNPQTGLID 71
V MALLQP D F L L G++ + P + A FFESL + L P+ G+ D
Sbjct 386 VLMALLQPAPETFDLFDDLILLSEGYMVYQG--PREDVIA---FFESLGFRLPPRKGVAD 440
Query 72 Y 72
+
Sbjct 441 F 441
Lambda K H
0.326 0.141 0.448
Gapped
Lambda K H
0.267 0.0410 0.140
Effective search space used: 2814663556
Database: egene_temp_file_orthology_annotation_similarity_blast_database_865
Posted date: Sep 17, 2011 11:19 AM
Number of letters in database: 82,071,388
Number of sequences in database: 164,496
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 11
Window for multiple hits: 40