BLASTP 2.2.24 [Aug-08-2010]
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eten_1973_orf5
(113 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
14,777,732 sequences; 5,058,227,080 total letters
Score E
Sequences producing significant alignments: (bits) Value
gi|157133750|ref|XP_001662996.1| nonsense-mediated mrna decay pr... 36 2.3
gi|94468868|gb|ABF18283.1| NMD protein [Aedes aegypti] 36 2.3
gi|242093730|ref|XP_002437355.1| hypothetical protein SORBIDRAFT... 35 2.8
>gi|157133750|ref|XP_001662996.1| nonsense-mediated mrna decay protein [Aedes aegypti]
gi|108881501|gb|EAT45726.1| nonsense-mediated mrna decay protein [Aedes aegypti]
Length = 510
Score = 35.8 bits (81), Expect = 2.3, Method: Composition-based stats.
Identities = 32/114 (28%), Positives = 47/114 (41%), Gaps = 20/114 (17%)
Query: 1 QQLLLHFRAHFASFRLGFIPSPRGG-------EVSARRARG--ERALPS---------VH 42
+Q++L +AH + LG PSP GG + AR+ + LP H
Sbjct: 179 EQMILKHKAHENT--LGIKPSPSGGLDFYYATDAHARKMVDFVQAVLPVKVTNSKKLISH 236
Query: 43 SFHKTETNDKYIYLVLIYPVISASLVRIGKWRRAQLGCTCTSVVRGHAAAAIQL 96
H N KY Y + + P+ SLV + K + QLG + A A+ L
Sbjct: 237 DIHSNSYNYKYSYALDVVPISKGSLVCLNKKLQQQLGHISPICLVTKVANAVHL 290
>gi|94468868|gb|ABF18283.1| NMD protein [Aedes aegypti]
Length = 510
Score = 35.8 bits (81), Expect = 2.3, Method: Composition-based stats.
Identities = 32/114 (28%), Positives = 47/114 (41%), Gaps = 20/114 (17%)
Query: 1 QQLLLHFRAHFASFRLGFIPSPRGG-------EVSARRARG--ERALPS---------VH 42
+Q++L +AH + LG PSP GG + AR+ + LP H
Sbjct: 179 EQMILKHKAHENT--LGIKPSPSGGLDFYYATDAHARKMVDFVQAVLPVKVTNSKKLISH 236
Query: 43 SFHKTETNDKYIYLVLIYPVISASLVRIGKWRRAQLGCTCTSVVRGHAAAAIQL 96
H N KY Y + + P+ SLV + K + QLG + A A+ L
Sbjct: 237 DIHSNSYNYKYSYALDVVPISKGSLVCLNKKLQQQLGHISPICLVTKVANAVHL 290
>gi|242093730|ref|XP_002437355.1| hypothetical protein SORBIDRAFT_10g025460 [Sorghum bicolor]
gi|241915578|gb|EER88722.1| hypothetical protein SORBIDRAFT_10g025460 [Sorghum bicolor]
Length = 470
Score = 35.4 bits (80), Expect = 2.8, Method: Composition-based stats.
Identities = 24/83 (28%), Positives = 42/83 (50%), Gaps = 10/83 (12%)
Query: 19 IPSPRGGEVSARRARGERALPSVHSFHKTETNDKYIYLVLIYPVISASLVRIGKWRRAQL 78
+PS +G SA ARG +P + FH +T+ K + ++ YP++ A+ GK+ + +
Sbjct: 123 LPSGKGAR-SADTARGIMLVPILSKFHSLKTSRKGLVAMIEYPLLEAA---TGKFSESNV 178
Query: 79 ------GCTCTSVVRGHAAAAIQ 95
GC +V G AA++
Sbjct: 179 LGVGGFGCVYKAVFDGGVTAAVK 201
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
Posted date: Jul 22, 2011 4:42 PM
Number of letters in database: 5,058,227,080
Number of sequences in database: 14,777,732
Lambda K H
0.329 0.140 0.427
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14777732
Number of Hits to DB: 1,020,903,146
Number of extensions: 33046471
Number of successful extensions: 87636
Number of sequences better than 10.0: 3
Number of HSP's gapped: 87904
Number of HSP's successfully gapped: 3
Length of query: 113
Length of database: 5,058,227,080
Length adjustment: 81
Effective length of query: 32
Effective length of database: 3,861,230,788
Effective search space: 123559385216
Effective search space used: 123559385216
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 76 (33.9 bits)