BLASTP 2.2.24 [Aug-08-2010]
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Query= Eace_0015_orf3
(314 letters)
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
14,777,732 sequences; 5,058,227,080 total letters
Score E
Sequences producing significant alignments: (bits) Value
gi|145505976|ref|XP_001438954.1| hypothetical protein [Parameciu... 42 0.17
gi|324114172|gb|EGC08145.1| type VI secretion protein [Escherich... 39 1.4
gi|325497722|gb|EGC95581.1| hypothetical protein ECD227_1819 [Es... 38 1.6
gi|218549310|ref|YP_002383101.1| hypothetical protein EFER_1972 ... 38 1.6
gi|148236153|ref|NP_001084637.1| protein kinase, cAMP-dependent,... 38 2.0
gi|239813377|ref|YP_002942287.1| FAD dependent oxidoreductase [V... 36 9.9
>gi|145505976|ref|XP_001438954.1| hypothetical protein [Paramecium tetraurelia strain d4-2]
gi|124406127|emb|CAK71557.1| unnamed protein product [Paramecium tetraurelia]
Length = 750
Score = 41.6 bits (96), Expect = 0.17, Method: Compositional matrix adjust.
Identities = 35/130 (26%), Positives = 58/130 (44%), Gaps = 33/130 (25%)
Query: 37 VFDTVVVKASSALSPQ----EQMRAARFVREKAKGELYRGAVVDSGYPTSFSVVAVGNET 92
VF V+VK + + PQ Q+ ++++A G F + NET
Sbjct: 183 VFSAVLVKDNQIIHPQPLPNTQVEETHQIQQEAHG---------------FDTLQAMNET 227
Query: 93 GTVYGVLK---GHVEDLTCTIEFLGASGLHENFVEAMLLADTLPFM-----------ERL 138
TV G K ++E++ ++ LG S ENF++ ++ DTL + E L
Sbjct: 228 VTVKGKKKMQDWNIEEVCIWLDCLGLSQYKENFIKNHMIGDTLHDLTDVELKEELGIEIL 287
Query: 139 GVRKVVLKSI 148
G RK++L+ I
Sbjct: 288 GHRKLILQQI 297
>gi|324114172|gb|EGC08145.1| type VI secretion protein [Escherichia fergusonii B253]
Length = 345
Score = 38.5 bits (88), Expect = 1.4, Method: Compositional matrix adjust.
Identities = 27/78 (34%), Positives = 38/78 (48%), Gaps = 11/78 (14%)
Query: 176 MVRDGSLPPPDTDCITLPRSFRAQYGVLKNFEGRH--RAPIDGKYLDVLELARMMVYEAP 233
V D SL P +D ++L + QY + +F G H ++P+ G YLD ELA + E
Sbjct: 56 FVADASLAFPKSDVVSLTKKENGQYAITVSFSGLHGSQSPLPGYYLD--ELALEVAQEEE 113
Query: 234 G-------FIKRRTQELF 244
G F R TQ L+
Sbjct: 114 GLAGFLDLFAHRWTQFLY 131
>gi|325497722|gb|EGC95581.1| hypothetical protein ECD227_1819 [Escherichia fergusonii ECD227]
Length = 316
Score = 38.1 bits (87), Expect = 1.6, Method: Compositional matrix adjust.
Identities = 27/78 (34%), Positives = 38/78 (48%), Gaps = 11/78 (14%)
Query: 176 MVRDGSLPPPDTDCITLPRSFRAQYGVLKNFEGRH--RAPIDGKYLDVLELARMMVYEAP 233
V D SL P +D ++L + QY + +F G H ++P+ G YLD ELA + E
Sbjct: 27 FVADASLAFPKSDVVSLTKKENGQYAITVSFSGLHGSQSPLPGYYLD--ELALEVAQEEE 84
Query: 234 G-------FIKRRTQELF 244
G F R TQ L+
Sbjct: 85 GLAGFLDLFAHRWTQFLY 102
>gi|218549310|ref|YP_002383101.1| hypothetical protein EFER_1972 [Escherichia fergusonii ATCC 35469]
gi|218356851|emb|CAQ89479.1| conserved hypothetical protein, Aec22 [Escherichia fergusonii ATCC
35469]
Length = 345
Score = 38.1 bits (87), Expect = 1.6, Method: Compositional matrix adjust.
Identities = 27/78 (34%), Positives = 38/78 (48%), Gaps = 11/78 (14%)
Query: 176 MVRDGSLPPPDTDCITLPRSFRAQYGVLKNFEGRH--RAPIDGKYLDVLELARMMVYEAP 233
V D SL P +D ++L + QY + +F G H ++P+ G YLD ELA + E
Sbjct: 56 FVADASLVFPKSDVVSLTKKENGQYAITVSFSGLHGSQSPLPGYYLD--ELALEVAQEEE 113
Query: 234 G-------FIKRRTQELF 244
G F R TQ L+
Sbjct: 114 GLAGFLDLFAHRWTQFLY 131
>gi|148236153|ref|NP_001084637.1| protein kinase, cAMP-dependent, regulatory, type II, beta [Xenopus
laevis]
gi|46249649|gb|AAH68931.1| MGC83177 protein [Xenopus laevis]
Length = 402
Score = 37.7 bits (86), Expect = 2.0, Method: Compositional matrix adjust.
Identities = 26/87 (29%), Positives = 46/87 (52%), Gaps = 7/87 (8%)
Query: 118 LHENFVEAMLLADTLPFMERLGVRKVVLKSIPICDAGMV---GLAAELGFVIKASSGDSH 174
L+ENF+E++ +L F ERL V V+ + CD + G A+ F+++ SG+
Sbjct: 250 LYENFIESLPFLKSLEFSERLKVVDVI-GTKTYCDEEQIIAQGDGADSFFIVE--SGEVR 306
Query: 175 LMVRDGSLPPPDTD-CITLPRSFRAQY 200
+ ++ S P +T+ + + R R QY
Sbjct: 307 ITMKSKSKPDAETNEAVEIARYIRGQY 333
>gi|239813377|ref|YP_002942287.1| FAD dependent oxidoreductase [Variovorax paradoxus S110]
gi|239799954|gb|ACS17021.1| FAD dependent oxidoreductase [Variovorax paradoxus S110]
Length = 453
Score = 35.8 bits (81), Expect = 9.9, Method: Compositional matrix adjust.
Identities = 25/93 (26%), Positives = 41/93 (44%), Gaps = 13/93 (13%)
Query: 166 IKASSGDSHLMVRDGSLPPPDTDCITLPRSFRAQYGVLKNFEGRHRAPIDGKYL------ 219
++A ++ R+G++ P+ D PR +RA ++N GR +D + L
Sbjct: 231 VQAQKPGAYRFPREGAILRPNID----PREWRANVTQIRNAAGRAMNGVDARELTEGELE 286
Query: 220 ---DVLELARMMVYEAPGFIKRRTQELFLQVGV 249
+ E R M E PGF E+ QVG+
Sbjct: 287 GRRQIAEYFRFMKAEVPGFAHSAIVEIAPQVGI 319
Database: All non-redundant GenBank CDS
translations+PDB+SwissProt+PIR+PRF excluding environmental samples
from WGS projects
Posted date: Jul 22, 2011 4:42 PM
Number of letters in database: 5,058,227,080
Number of sequences in database: 14,777,732
Lambda K H
0.321 0.136 0.389
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 14777732
Number of Hits to DB: 2,946,963,253
Number of extensions: 118618262
Number of successful extensions: 283644
Number of sequences better than 10.0: 10
Number of HSP's gapped: 284781
Number of HSP's successfully gapped: 10
Length of query: 314
Length of database: 5,058,227,080
Length adjustment: 139
Effective length of query: 175
Effective length of database: 3,004,122,332
Effective search space: 525721408100
Effective search space used: 525721408100
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 81 (35.8 bits)