bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Microviridae_proteins.fasta 575 sequences; 147,441 total letters Query= Contig-35_CDS_annotation_glimmer3.pl_2_2 Length=53 Score E Sequences producing significant alignments: (Bits) Value Pichovirinae_Bourget_523_Microviridae_AG0337_hypothetical.prote... 19.2 3.6 Gokush_JCVI_001_Microviridae_AG023_putative.VP3 18.9 5.1 Alpavirinae_Human_gut_32_015_Microviridae_AG0214_putative.VP4 18.5 8.0 Alpavirinae_Human_feces_B_020_Microviridae_AG0353_putative.gpH 18.1 8.8 > Pichovirinae_Bourget_523_Microviridae_AG0337_hypothetical.protein.BACEGG.02723 Length=307 Score = 19.2 bits (38), Expect = 3.6, Method: Composition-based stats. Identities = 8/20 (40%), Positives = 11/20 (55%), Gaps = 0/20 (0%) Query 2 TNFHNAIGAGRKAGEKLRGN 21 TNF ++ R A E+ R N Sbjct 166 TNFQYSVEGARLANEQTRAN 185 > Gokush_JCVI_001_Microviridae_AG023_putative.VP3 Length=142 Score = 18.9 bits (37), Expect = 5.1, Method: Compositional matrix adjust. Identities = 6/18 (33%), Positives = 11/18 (61%), Gaps = 0/18 (0%) Query 1 VTNFHNAIGAGRKAGEKL 18 +T++H A+ A R+ E Sbjct 66 ITDYHTAMNAIRRGDEAF 83 > Alpavirinae_Human_gut_32_015_Microviridae_AG0214_putative.VP4 Length=489 Score = 18.5 bits (36), Expect = 8.0, Method: Compositional matrix adjust. Identities = 5/14 (36%), Positives = 12/14 (86%), Gaps = 0/14 (0%) Query 23 NKAMDNIKNGHGID 36 ++A++NIK +G++ Sbjct 75 DEAIENIKKQYGVE 88 > Alpavirinae_Human_feces_B_020_Microviridae_AG0353_putative.gpH Length=352 Score = 18.1 bits (35), Expect = 8.8, Method: Composition-based stats. Identities = 6/10 (60%), Positives = 9/10 (90%), Gaps = 0/10 (0%) Query 22 MNKAMDNIKN 31 +NKAM+N+ N Sbjct 159 LNKAMENLTN 168 Lambda K H a alpha 0.313 0.133 0.382 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 3725848