bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-20_CDS_annotation_glimmer3.pl_2_6
Length=313
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_C_016_Microviridae_AG0273_putative.VP4 602 0.0
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 138 3e-39
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 129 1e-35
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 126 4e-35
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 126 5e-35
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 125 9e-35
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 122 7e-34
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 118 3e-32
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 115 3e-31
Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 112 2e-30
> Alpavirinae_Human_feces_C_016_Microviridae_AG0273_putative.VP4
Length=304
Score = 602 bits (1552), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 283/304 (93%), Positives = 293/304 (96%), Gaps = 0/304 (0%)
Query 10 MTNTEIVDYAKTYYGCFWPPDYYLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPPGTCLF 69
MTN EIVDYA+T+YGCFWPPDY LEVPCGYCHSCQKSYNNQYRIRLLYELRKYP GTCLF
Sbjct 1 MTNAEIVDYARTFYGCFWPPDYNLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPSGTCLF 60
Query 70 VTLTFDDDNLKKFSKDTNKAVRLFLDRLRKDYGKQIRHWFVCEFGTLYGRPHYHGILFDV 129
VTLTFDDDNLKKFSKDTNKAVRLFLDRLRKDYGKQIRHWFVCEFGTL RPHYHGILF+V
Sbjct 61 VTLTFDDDNLKKFSKDTNKAVRLFLDRLRKDYGKQIRHWFVCEFGTLRCRPHYHGILFNV 120
Query 130 PQTLIDGYSPDVPGHHPLLASRWKYGFVFVGYVSDETCSYITKYVTKSINGDKVRPRIIS 189
PQ LIDGYSPDVPGHHPLLASRWKYGFVFVGYVS+ETCSYITKYVTKSINGDKVRPR+IS
Sbjct 121 PQALIDGYSPDVPGHHPLLASRWKYGFVFVGYVSEETCSYITKYVTKSINGDKVRPRVIS 180
Query 190 SFGIGSNYFDTEESTLHKLGGQRYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRFV 249
SFGIGSNY DTEES+LHKLG QRYQPFMVLNGFQQAMPRYYYNK+FSDVDKQN+V+DRF+
Sbjct 181 SFGIGSNYLDTEESSLHKLGNQRYQPFMVLNGFQQAMPRYYYNKLFSDVDKQNMVIDRFI 240
Query 250 NPPVEFSWQGQKFSSKLERDEMRRSTLNQNITSGLTPALPLPHTERVSSFDRFKENMDKN 309
NPPVEFSWQGQKF SKLERDEMRRSTLNQNI SGLTP LPLPHTERVSSFDRFKENMDKN
Sbjct 241 NPPVEFSWQGQKFGSKLERDEMRRSTLNQNIASGLTPVLPLPHTERVSSFDRFKENMDKN 300
Query 310 KEFK 313
KEFK
Sbjct 301 KEFK 304
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 138 bits (347), Expect = 3e-39, Method: Compositional matrix adjust.
Identities = 82/230 (36%), Positives = 128/230 (56%), Gaps = 28/230 (12%)
Query 32 YLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPPGTCLFVTLTFDDDNLKKF-----SKDT 86
Y+ CG C C++ Q+ +R+ ELR+ P F+TLT DD+N K SKD
Sbjct 33 YVTAACGKCMECRQQKQRQWLVRMSEELRQNP--NAYFMTLTIDDENYNKLANICNSKDN 90
Query 87 N----KAVRLFLDRLRKDYGKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGYSPDV 141
N KAVRL L+R+RK GK I+HWF+ E G R H HGI++ +
Sbjct 91 NEIATKAVRLMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGI------------ 138
Query 142 PGHHPLLASRWKYGFVFVG-YVSDETCSYITKYVTK-SINGDKVRPRIISSFGIGSNYFD 199
G L++ +W YGFV+ G +V++ T +YITKY+TK I+ +++ S GIG+ Y
Sbjct 139 -GTDQLISEKWNYGFVYTGNFVNEATINYITKYMTKVDIDHPDFVGQVLCSKGIGAGYTK 197
Query 200 TEESTLHK-LGGQRYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 248
E++ HK G+ + + + NG + +P YY N++FS+ +++ + LD+
Sbjct 198 REDANNHKYTKGKTNETYRLRNGAKINLPIYYRNQLFSEEEREMLFLDKI 247
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 129 bits (323), Expect = 1e-35, Method: Compositional matrix adjust.
Identities = 81/236 (34%), Positives = 116/236 (49%), Gaps = 38/236 (16%)
Query 32 YLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPPGTCLFVTLTFDDDNLKKFSKDT----- 86
Y+ V CG C C+K ++R+RL E+ F TLT D +N+KK T
Sbjct 33 YIPVKCGCCIECRKEKQREWRVRLEEEMH---SNFGYFTTLTIDQENIKKIESITGLKWE 89
Query 87 -------NKAVRLFLDRLRKDYGKQIRHWFVCEFGTLYGRPHYHGILFDVPQTLIDGYSP 139
KA+RLFL+R RKD GK IRHW V E G R H HGI F
Sbjct 90 ENPNEIATKALRLFLERTRKDTGKSIRHWCVTELGEKNNRIHLHGIFFGQKSA------- 142
Query 140 DVPGHHPLLASRWKYGFVFV-GYVSDETCSYITKYVTK-SINGDKVRPRIISSFGIGSNY 197
L+ W YGF+F+ GY + ++ +YITKY+ K I + + +++S GIG Y
Sbjct 143 ------ELIRKHWNYGFIFIGGYCNSKSINYITKYMLKVDIKHPEFKQIVLASSGIGKGY 196
Query 198 FDTEESTLHKLGGQRYQPFMVL-----NGFQQAMPRYYYNKIFSDVDKQNIVLDRF 248
D + K Q Y+ V NG + AMP+YY NKIF++ +++ + ++
Sbjct 197 IDRLDYLWQK---QNYKNINVATYTFRNGTKMAMPKYYKNKIFTEKEREKMWINNL 249
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 126 bits (317), Expect = 4e-35, Method: Compositional matrix adjust.
Identities = 74/230 (32%), Positives = 121/230 (53%), Gaps = 28/230 (12%)
Query 32 YLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPPGTCLFVTLTFDDDNLKKFSKDTN---- 87
Y+ CG C C++ Q+ +R+ ELR+ P F+TLT DD + + S N
Sbjct 33 YITAACGKCMECRQQKQRQWLVRMSEELRQEP--NAYFITLTIDDKSYSELSNTYNITDN 90
Query 88 -----KAVRLFLDRLRKDYGKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGYSPDV 141
KA+RL L+R+RK GK I+HWF+ E G R H HGI++ +
Sbjct 91 NEIATKAIRLCLERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGI------------ 138
Query 142 PGHHPLLASRWKYGFVFVG-YVSDETCSYITKYVTKSINGDK-VRPRIISSFGIGSNYFD 199
G L+ S+W YG F G +V+++T YITKY+TK K +++ S GIG+ Y
Sbjct 139 -GTDKLITSKWNYGITFTGFFVNEKTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIK 197
Query 200 TEESTLHKLG-GQRYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 248
+++ H G+ + + + NG + +P YY N++F++ +++ + LD+
Sbjct 198 RDDAKKHTYKRGETIETYRLRNGSKINLPIYYRNQLFTEEEREALFLDKI 247
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 126 bits (316), Expect = 5e-35, Method: Compositional matrix adjust.
Identities = 79/237 (33%), Positives = 126/237 (53%), Gaps = 34/237 (14%)
Query 28 PPDYYLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPPGTCLFVTLTFDDDN---LKKF-- 82
P Y+ CG C C+K ++ +R+ ELR P F+TLT D+N LK
Sbjct 29 PRTAYITAACGKCLECRKQKQREWLVRMSEELRTEP--NAYFMTLTISDENYEILKNICK 86
Query 83 SKDTN----KAVRLFLDRLRKDYGKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGY 137
S+D N KA+RL L+R+RK GK IRHWF+ E G R H HGI++ +
Sbjct 87 SEDENTIATKAIRLMLERIRKKIGKSIRHWFITELGHEKTERLHLHGIVWGI-------- 138
Query 138 SPDVPGHHPLLASRWKYGFVFVG-YVSDETCSYITKYVTKSINGDKVRP----RIISSFG 192
G L+ +W YG + G +V+++T +YITKY+TK D+ P +++ S G
Sbjct 139 -----GTDQLIREKWNYGITYTGNFVNEKTINYITKYMTKI---DEEHPNFVGKVLCSKG 190
Query 193 IGSNYFDTEESTLHKL-GGQRYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 248
IG+ Y ++ HK G+ + + + NG + +P YY NK+F++ +++ + +D+
Sbjct 191 IGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFTEKERELLFIDKI 247
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 125 bits (314), Expect = 9e-35, Method: Compositional matrix adjust.
Identities = 78/237 (33%), Positives = 126/237 (53%), Gaps = 34/237 (14%)
Query 28 PPDYYLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPPGTCLFVTLTFDDDN---LKKF-- 82
P Y+ CG C C+K ++ +R+ ELR P F+TLT D+N LK
Sbjct 29 PRTAYITAACGKCLECRKQKQREWLVRMSEELRTEP--NAYFMTLTISDENYEILKNICK 86
Query 83 SKDTN----KAVRLFLDRLRKDYGKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGY 137
S+D N KA+RL L+R+RK GK I+HWF+ E G R H HGI++ +
Sbjct 87 SEDDNTIATKAIRLMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGI-------- 138
Query 138 SPDVPGHHPLLASRWKYGFVFVG-YVSDETCSYITKYVTKSINGDKVRP----RIISSFG 192
G L+ +W YG + G YV+++T +Y+TKY+TK D+ P +++ S G
Sbjct 139 -----GTDQLIEEKWNYGITYTGNYVNEKTINYVTKYMTKI---DEKHPDFVGKVLCSRG 190
Query 193 IGSNYFDTEESTLHKL-GGQRYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 248
IG+ Y ++ HK G+ + + + NG + +P YY NK+F++ +++ + +D+
Sbjct 191 IGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFTEKERELLFIDKI 247
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 122 bits (306), Expect = 7e-34, Method: Compositional matrix adjust.
Identities = 75/235 (32%), Positives = 120/235 (51%), Gaps = 28/235 (12%)
Query 32 YLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPPGTCLFVTLTFDDDNLKKFSKD------ 85
Y+ CG C+ C+K +++RL E+R P +FVTLT D++ +K
Sbjct 33 YVTAACGKCYECRKQKGRAWQVRLSEEVRSDPNA--IFVTLTISDESWEKIKNTYIQLSD 90
Query 86 ---TNKAVRLFLDRLRKDYGKQIRHWFVCEF-GTLYGRPHYHGILFDVPQTLIDGYSPDV 141
K VRLFL+R+RK K ++HW E GT R H HG+++
Sbjct 91 EDCIKKMVRLFLERVRKKTKKSLKHWLTTERGGTNTERYHLHGLIWG------------- 137
Query 142 PGHHPLLASRWKYGFVFVG-YVSDETCSYITKYVTKSINGDK-VRPRIISSFGIGSNYFD 199
L S W+YGFVF+G +V++ T +YITKY+TK+ K P + S GIG Y
Sbjct 138 ENTEALTKSLWQYGFVFIGTFVNECTVNYITKYITKTDKKHKDFEPITLCSAGIGKGYLS 197
Query 200 TEESTLHKL-GGQRYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRFVNPPV 253
+S L++ G+ + + + NG + +P YY NK+++D +++ + L++ V
Sbjct 198 RSDSELNRFREGKTTETYRLRNGTKLNLPIYYRNKLYTDEEREKLFLEKIKKGKV 252
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 118 bits (295), Expect = 3e-32, Method: Compositional matrix adjust.
Identities = 72/234 (31%), Positives = 123/234 (53%), Gaps = 38/234 (16%)
Query 32 YLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPPGTCLFVTLTFDDDNLKKFSKDTNK--- 88
Y+ CG C C+K +RIRL EL+ P LF+TLTF+++N ++ S + K
Sbjct 31 YVPTKCGKCIECRKEKARNWRIRLAEELKNNP--NALFITLTFNEENYQRLSWELFKKSK 88
Query 89 --------------AVRLFLDRLRKDYGKQIRHWFVCEFGTLYGRPHYHGILFDVPQTLI 134
AVR +L+R+RK K IRHW V E G YGR H HGI++ P+ I
Sbjct 89 DNLNYTEQNEMCKTAVRRWLERIRKKTKKSIRHWMVTEKGEDYGRIHLHGIVW-CPKERI 147
Query 135 DGYSPDVPGHHPLLASRWKYGFVFVG-YVSDETCSYITKYVTKSING-DKVRPRIISSFG 192
+ +W YG+ ++G YV++ T +Y+TKY+ K R +++ S G
Sbjct 148 E---------------QWGYGYTYIGDYVNETTIAYVTKYMLKICEKWPDFRGKVMCSAG 192
Query 193 IGSNYFDTEESTLHKL-GGQRYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVL 245
IG Y + + ++ G + + + + NG + +P+YY++KI+++ +++ + +
Sbjct 193 IGCRYETSYNAKRNRYRGKETKETYKLENGRELPLPKYYHDKIYTEEEREKLWI 246
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 115 bits (287), Expect = 3e-31, Method: Compositional matrix adjust.
Identities = 70/220 (32%), Positives = 116/220 (53%), Gaps = 28/220 (13%)
Query 42 SCQKSYNNQYRIRLLYELRKYPPGTCLFVTLTFDDDNLKKFSKDTN---------KAVRL 92
C++ Q+ +R+ ELR+ P F+TLT DD + + S N KA+RL
Sbjct 2 ECRQQKQRQWLVRMSEELRQEP--NAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRL 59
Query 93 FLDRLRKDYGKQIRHWFVCEFG-TLYGRPHYHGILFDVPQTLIDGYSPDVPGHHPLLASR 151
L+R+RK GK I+HWF+ E G R H HGI++ + G L+ ++
Sbjct 60 CLERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGI-------------GTDKLITNK 106
Query 152 WKYGFVFVG-YVSDETCSYITKYVTKSINGDK-VRPRIISSFGIGSNYFDTEESTLHKLG 209
W YG F G +V+++T YITKY+TK K +++ S GIG+ Y +++ H
Sbjct 107 WNYGITFTGFFVNEKTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYK 166
Query 210 -GQRYQPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDRF 248
G+ + + + NG + +P YY N++F++ +K+ + LD+
Sbjct 167 PGETIETYRLRNGSKINLPIYYRNQLFTEEEKEALFLDKI 206
> Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4
Length=316
Score = 112 bits (281), Expect = 2e-30, Method: Compositional matrix adjust.
Identities = 75/227 (33%), Positives = 124/227 (55%), Gaps = 27/227 (12%)
Query 31 YYLEVPCGYCHSCQKSYNNQYRIRLLYELRKYPPGTCLFVTLTFDDDNLKKFS---KDTN 87
+Y+ V CG C C++ + +RL ELR G LFVTLTF++++ K+ + K+ N
Sbjct 32 FYVPVKCGKCIECRQQKQRAWIVRLSEELRS-GKGAGLFVTLTFNEESYKELAAITKNEN 90
Query 88 KAVRLFLDRL----RKDYGKQIRHWFVCEFGTLYGRPHYHGILFDVPQTLIDGYSPDVPG 143
RL L R+ R+ Y IRHW V E G GR H HGI++ P + I+ Y
Sbjct 91 DMCRLALYRMNENYRQKYKHTIRHWCVTEIGDD-GRIHIHGIMW-CPASDIERY------ 142
Query 144 HHPLLASRWKYGFVFVG-YVSDETCSYITKYVTKSINGDK-VRPRIISSFGIGSNYFDTE 201
WKYG++++G +V+++T YITKY+ K DK P+++ S GIG NY D
Sbjct 143 --------WKYGYIYIGRFVNEQTILYITKYMLKYTPVDKNFEPKVLCSKGIGINYLDRL 194
Query 202 ESTLHKLGGQRY-QPFMVLNGFQQAMPRYYYNKIFSDVDKQNIVLDR 247
+S + + +M+ +G + +P YY KI+++ +++ + +++
Sbjct 195 DSKRNTYRENNTDESYMLRSGRKINLPDYYKRKIYTEEEREKLWIEK 241
Lambda K H a alpha
0.323 0.141 0.447 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 26543545