bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Microviridae_proteins.fasta 575 sequences; 147,441 total letters Query= Contig-17_CDS_annotation_glimmer3.pl_2_7 Length=49 Score E Sequences producing significant alignments: (Bits) Value Gokush_JCVI_001_Microviridae_AG021_putative.VP4 20.0 1.7 Alpavirinae_Human_feces_B_020_Microviridae_AG0348_hypothetical.... 18.9 4.0 Gokush_Human_feces_A_019_Microviridae_AG0436_putative.VP4 18.9 4.7 Gokush_Human_feces_E_017_Microviridae_AG0124_putative.VP4 18.1 8.4 > Gokush_JCVI_001_Microviridae_AG021_putative.VP4 Length=297 Score = 20.0 bits (40), Expect = 1.7, Method: Composition-based stats. Identities = 7/23 (30%), Positives = 13/23 (57%), Gaps = 5/23 (22%) Query 5 HSSYIYSNHCYNYDLMCTIVYNE 27 H + +Y N+C+ T+ YN+ Sbjct 53 HEASLYENNCF-----VTLTYND 70 > Alpavirinae_Human_feces_B_020_Microviridae_AG0348_hypothetical.protein Length=81 Score = 18.9 bits (37), Expect = 4.0, Method: Compositional matrix adjust. Identities = 7/17 (41%), Positives = 10/17 (59%), Gaps = 0/17 (0%) Query 16 NYDLMCTIVYNEYEYSK 32 N+DL+ +VY E K Sbjct 47 NWDLVSALVYALKEADK 63 > Gokush_Human_feces_A_019_Microviridae_AG0436_putative.VP4 Length=338 Score = 18.9 bits (37), Expect = 4.7, Method: Composition-based stats. Identities = 7/18 (39%), Positives = 12/18 (67%), Gaps = 0/18 (0%) Query 32 KKQQQCNATDGTNNNVKR 49 K+Q+Q A D T N +++ Sbjct 295 KRQRQQKAMDSTKNKMEQ 312 > Gokush_Human_feces_E_017_Microviridae_AG0124_putative.VP4 Length=338 Score = 18.1 bits (35), Expect = 8.4, Method: Composition-based stats. Identities = 6/18 (33%), Positives = 12/18 (67%), Gaps = 0/18 (0%) Query 32 KKQQQCNATDGTNNNVKR 49 K+Q+Q + D T N +++ Sbjct 295 KRQRQQKSMDSTKNKMEK 312 Lambda K H a alpha 0.315 0.128 0.400 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 3639357