bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-5_CDS_annotation_glimmer3.pl_2_6
Length=450
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_gut_21_005_Microviridae_AG013_putative.VP4 262 2e-83
Alpavirinae_Human_feces_C_010_Microviridae_AG0198_putative.VP4 259 2e-82
Alpavirinae_Human_feces_B_007_Microviridae_AG067_putative.VP4 170 2e-48
Pichovirinae_Pavin_723_Microviridae_AG0324_putative.VP4 105 3e-27
Gokush_Human_feces_E_009_Microviridae_AG0282_putative.VP4 105 3e-27
Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4 99.8 3e-25
Pichovirinae_59_Coral_002_Microviridae_AG0338_putative.VP4 97.1 2e-24
Gokush_Human_feces_E_010_Microviridae_AG0123_putative.VP4 95.5 8e-24
Gokush_Human_feces_C_014_Microviridae_AG0151_putative.VP4 93.6 5e-23
Gokush_gi|47566147|ref|YP_022485.1|_nonstructural_protein_[Chla... 93.2 7e-23
> Alpavirinae_Human_gut_21_005_Microviridae_AG013_putative.VP4
Length=451
Score = 262 bits (669), Expect = 2e-83, Method: Compositional matrix adjust.
Identities = 139/313 (44%), Positives = 184/313 (59%), Gaps = 27/313 (9%)
Query 1 MYNLYCEHPVIIRHPRLKDLLPVFGSYRTPNGLYDLTPSQSHTWKYRFPEWLFSARKCGV 60
M N+YCE P +I HP L+ + ++ P+G+Y + + K V
Sbjct 1 MNNIYCEDPKVIWHPHASKLIQKYRTFTMPSGVYHGS--------------VLHVNKNHV 46
Query 61 TLDNIDDFQIINLRTGEISPMFMAVPCGKCILCRDKKKREWSFRATCENVFSTSIPIFVT 120
+NID + I+N TGE PMF+ VPC KC+LC +KK ++WSFRA CE+ S F+T
Sbjct 47 NKNNIDKYTIVNPATGETFPMFLIVPCNKCVLCNEKKAQQWSFRALCESYTSNKQAYFIT 106
Query 121 LTYNNKHLPKYGVFKEEIQLFMKRLRIRLDRLGYKHQIRYFFCSEYGSKSGRPHYHAIFW 180
LTYNN+HLPK GVF EEIQLF KRLR +LDR G H +RY SEYG S RPHYH I W
Sbjct 107 LTYNNEHLPKNGVFPEEIQLFFKRLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIILW 166
Query 181 NFPRDGALSNIWNVVKMIEKAW-----SYN--GEP----LGFAYAVPCDKGAIGYVMKYM 229
NFP + + ++ + +IE W YN G P +GFAY VP G I YVMKYM
Sbjct 167 NFPDN--FESAYSRLTLIESCWRRPTGKYNPDGSPVTRSIGFAYCVPVINGGINYVMKYM 224
Query 230 SKIPRIPDNMNNVFFLSSRKDGGIGAAYARRLMPFYRANSQCLDITVCDPYSGMSTTVTL 289
K + P+ MN F L+SRK+GGIG+AYA +L FY D++V + Y+G + T L
Sbjct 225 GKREKSPEGMNPTFMLASRKNGGIGSAYAEQLRAFYEQQPDTCDMSVINIYTGQTLTTML 284
Query 290 PEYFKRLYYPANS 302
P Y++ + P+ S
Sbjct 285 PRYYRMKFMPSPS 297
> Alpavirinae_Human_feces_C_010_Microviridae_AG0198_putative.VP4
Length=451
Score = 259 bits (662), Expect = 2e-82, Method: Compositional matrix adjust.
Identities = 138/313 (44%), Positives = 183/313 (58%), Gaps = 27/313 (9%)
Query 1 MYNLYCEHPVIIRHPRLKDLLPVFGSYRTPNGLYDLTPSQSHTWKYRFPEWLFSARKCGV 60
M N+YCE P +I HP L+ + ++ P+G+Y + + K V
Sbjct 1 MNNIYCEDPKVIWHPHASKLIQKYRTFTMPSGVYYGS--------------VLHVNKNHV 46
Query 61 TLDNIDDFQIINLRTGEISPMFMAVPCGKCILCRDKKKREWSFRATCENVFSTSIPIFVT 120
+NID + ++N TGE PMF+ VPC KC LC +KK ++WSFRA CE+ S F+T
Sbjct 47 NKNNIDKYTVVNPATGETFPMFLIVPCNKCALCNEKKAQQWSFRALCESYTSNKQAYFIT 106
Query 121 LTYNNKHLPKYGVFKEEIQLFMKRLRIRLDRLGYKHQIRYFFCSEYGSKSGRPHYHAIFW 180
LTYNN+HLPK GVF EEIQLF KRLR +LDR G H +RY SEYG S RPHYH I W
Sbjct 107 LTYNNEHLPKNGVFPEEIQLFFKRLRTKLDRRGISHNLRYIAVSEYGHWSKRPHYHIILW 166
Query 181 NFPRDGALSNIWNVVKMIEKAW-----SYN--GEP----LGFAYAVPCDKGAIGYVMKYM 229
NFP + + ++ + +IE W YN G P +GFAY VP G I YVMKY+
Sbjct 167 NFPDN--FESAYSRLTLIESCWRRPTGEYNPDGSPVTRSIGFAYCVPVINGGINYVMKYL 224
Query 230 SKIPRIPDNMNNVFFLSSRKDGGIGAAYARRLMPFYRANSQCLDITVCDPYSGMSTTVTL 289
K P+ MN+ F L+SRK+GGIG+AYA +L FY D++V + Y+G S T L
Sbjct 225 GKRECAPEGMNHTFMLASRKNGGIGSAYAEQLRAFYEQQPDTCDMSVINIYTGQSLTTML 284
Query 290 PEYFKRLYYPANS 302
P Y++ + P+ S
Sbjct 285 PRYYRMKFMPSTS 297
> Alpavirinae_Human_feces_B_007_Microviridae_AG067_putative.VP4
Length=515
Score = 170 bits (430), Expect = 2e-48, Method: Compositional matrix adjust.
Identities = 136/503 (27%), Positives = 220/503 (44%), Gaps = 95/503 (19%)
Query 3 NLYCEHPVIIRHPRLK----DLLPVFGSYRTPNGLYDLTPSQSHTWKYRFPEWLFSARKC 58
++ C HPVI+ +P + D +F R + + + W +S +K
Sbjct 2 DIKCLHPVILINPEARKKALDFDRIFIRERCQCWIME---------TFVLEPWQYSPKKW 52
Query 59 GVTLDNIDDFQIINLRTGEISPMFMAVPCGKCILCRDKKKREWSFRATCENVFSTSIPIF 118
+ L +I+ ++N TG++ P+++ VPCG C++CR +K + RA E + S P+F
Sbjct 53 SIKLSDIEHCYLLNSETGDMIPLYIVVPCGSCVICRKRKANALATRAIMETETTGSAPLF 112
Query 119 VTLTYNNKHLPK--YG---VFKEEIQLFMKRLRIRLDRLGYKHQIRYFFCSEYGSKSGRP 173
+TLTYN +HLPK YG + K ++QLF KRLR LD H +RY C EYG+ + RP
Sbjct 113 ITLTYNPEHLPKNEYGYETLRKSDLQLFFKRLRSLLDNQSISHSLRYLACGEYGTNTKRP 172
Query 174 HYHAIFWNFPRDGALSNIWNVVKMIEKAWSY-----NGE--------------------- 207
HYH + W FP +I V I+KAWSY NG+
Sbjct 173 HYHLLLWGFPAT-HFKDILKVQAFIQKAWSYFQSDENGKRIPYYSKCRSCPLNQYKDRNS 231
Query 208 -------------------------PLGFAYAVPCDKGAIGYVMKYMSKIPRIPDNMNNV 242
P+G +P + GA Y+ KYM K P + +
Sbjct 232 CSAVAHLCAGARLRYPSGAFIYRRYPIGSIKVLPANSGAPAYITKYMVKGSNAPHSTCEL 291
Query 243 -FFLSSRKDGGIGAAYARRLMPFYRANSQCLDITVCDPYSGMSTTVTLP--EYFKRLYYP 299
F ++S + GGIG+AY R +NS + V D +G +P + K P
Sbjct 292 PFRVASNRGGGIGSAYIRARKDEILSNSSLEALPVVDRVTGSGKIFYMPIDSWVKSTLIP 351
Query 300 ANSSVLSKEVRDAFKKLCDCIS---------------DRYTIHCAGNYTDKLRFSDIEKK 344
+ SS L + + + C S DR+ YTD+ F D K+
Sbjct 352 SPSSCLKTKEYETVRDFCYTFSLFNQAVQQLYRLYPMDRFVDGELQYYTDE--FCDPFKR 409
Query 345 VLRKYSFLSPKICKY--PIGKKMDYYTQIPWQALDDRYVANECE-IASLCRFLMLESIDE 401
+K++ + +Y PI ++ I + L + Y+ E + +L R ++ ID+
Sbjct 410 --KKWAEAYSHVREYTPPIVPHINCGNWIKTRELFNEYIYTLTERLDTLARKVLEIKIDD 467
Query 402 TWFKIRDEILQKRQRALNAKFSN 424
++F+ R++ L+ R K+ +
Sbjct 468 SYFRSREQYLKSRVEIFKRKYGD 490
> Pichovirinae_Pavin_723_Microviridae_AG0324_putative.VP4
Length=278
Score = 105 bits (261), Expect = 3e-27, Method: Compositional matrix adjust.
Identities = 65/179 (36%), Positives = 97/179 (54%), Gaps = 26/179 (15%)
Query 82 FMAVPCGKCILCRDKKKREWSFRATCENVFSTSIPIFVTLTYNNKHLP-----KYGVFKE 136
++ VPC KC C ++ WS+R E S S +FVTLTY+ +++P + K
Sbjct 19 YIPVPCSKCPPCMKRRTSGWSYRLIKEGERSIS-ALFVTLTYDTEYVPITEKGYMNLDKT 77
Query 137 EIQLFMKRLRIRLDRLGYKHQIRYFFCSEYGSKSGRPHYHAIFWNFPRDGALSNIWNVVK 196
+IQ F KRLR +L K +I+Y+ C EYG+K RPHYH I +N ++
Sbjct 78 DIQKFFKRLR----KLS-KTKIKYYVCGEYGTKKMRPHYHIILFNANKEH---------- 122
Query 197 MIEKAWSYNGEPLGFAYAVPCDKGAIGYVMKYMSKIPRIP----DNMNNVFFLSSRKDG 251
I+KAW N +PLG + ++ +IGY +KYM+K +IP D+ F L S++ G
Sbjct 123 -IQKAWILNNKPLGTMHIGQVNEASIGYTLKYMTKKGKIPLHYNDDRQKEFSLMSKRLG 180
> Gokush_Human_feces_E_009_Microviridae_AG0282_putative.VP4
Length=340
Score = 105 bits (263), Expect = 3e-27, Method: Compositional matrix adjust.
Identities = 89/265 (34%), Positives = 122/265 (46%), Gaps = 47/265 (18%)
Query 78 ISPMFMAVPCGKCILCRDKKKREWSFRATCENVFSTSIPIFVTLTYNNKHLPK------- 130
+S F+ +PCGKC+ CR REW+ R E + +S FVTLTY+++HLP+
Sbjct 54 VSRDFIEIPCGKCVGCRLDYSREWANRCMLE-LEDSSNAWFVTLTYDDQHLPRSAYVEPE 112
Query 131 -------YGVFKEEIQLFMKRLRIRLDRLGYKHQIRYFFCSEYGSKSGRPHYHAIFWN-- 181
Y + K + QLFMKRLR ++IR+F EYGS S RPHYHAI +N
Sbjct 113 TGEAFASYSLRKTDFQLFMKRLRYYFP----DNKIRFFAAGEYGSHSHRPHYHAILYNVD 168
Query 182 -----FPRDGALSNIWNVVKMIEKAWSYNGEPLGFAYAVPCDKGAIGYVMKYMSKIPRIP 236
F + +I+ K ++ AW+ GFA + YV +Y K
Sbjct 169 FDDLEFYKKSLNGDIYWNSKKLDAAWN-----KGFAVIGEVTWQSCAYVARYCMKKADGV 223
Query 237 D-------NMNNVFFLSSRKDGGIGAAYARRLMPFYRANSQCLDITVCDPYSGMSTTVTL 289
D N+ F L SRK GIG Y + Y Q I V P G +T+
Sbjct 224 DASYYEHFNIEPEFTLMSRKP-GIGRMYLDKHPDLY----QYQKIFVSTPQGG--KEITI 276
Query 290 PEYFKRLYYPANSSVLS--KEVRDA 312
P+YF R+ N ++ KE R A
Sbjct 277 PKYFDRIVAQENPEMIEALKEKRKA 301
> Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4
Length=305
Score = 99.8 bits (247), Expect = 3e-25, Method: Compositional matrix adjust.
Identities = 61/157 (39%), Positives = 83/157 (53%), Gaps = 20/157 (13%)
Query 85 VPCGKCILCRDKKKREWSFRATCENVFSTSIPIFVTLTYNNKHLPKYGVFK-EEIQLFMK 143
+PCG+CI CR K REW+ R E S +F+TLTY++ HLP+ G E QLFMK
Sbjct 40 IPCGQCIGCRLSKSREWAARCVVE-AKSHKNNMFLTLTYDDAHLPEDGSLHYEHFQLFMK 98
Query 144 RLR-IRLDRLGYKHQIRYFFCSEYGSKSGRPHYHAIFWNFPRDGALSNIWNV-------- 194
R+R + R G Q+R+F C EYG K GRPHYHAI F +W++
Sbjct 99 RMRKYFMSRFG--QQLRFFMCGEYGDKLGRPHYHAII--FGVTFVDKQLWSIRRGNNLYR 154
Query 195 VKMIEKAWSYNGEPLGFAYAVPCDKGAIGYVMKYMSK 231
+ +EK W P GF+ + YV +Y++K
Sbjct 155 SRTLEKLW-----PYGFSSIGAVNFETAAYVARYVTK 186
> Pichovirinae_59_Coral_002_Microviridae_AG0338_putative.VP4
Length=274
Score = 97.1 bits (240), Expect = 2e-24, Method: Compositional matrix adjust.
Identities = 69/221 (31%), Positives = 118/221 (53%), Gaps = 30/221 (14%)
Query 81 MFMAVPCGKCILCRDKKKREWSFRATCENVFSTSIPIFVTLTYNNKHLPKYG---VFKEE 137
+ ++VPCGKC+ C ++ +WSFR E ++S F+TLTY N + + G + K +
Sbjct 21 LMVSVPCGKCLACTKRRASQWSFRLN-EEAKTSSSACFITLTYENAPVSENGFRTLNKRD 79
Query 138 IQLFMKRLRIRLDRLGYKHQIRYFFCSEYGSKSGRPHYHAIFWNFPRDGALSNIWNVVKM 197
QLF+KRLR + ++++Y+ C EYG+++ RPHYHAI +N P+ S I + K+
Sbjct 80 FQLFLKRLRKKCP----TNKLKYYACGEYGTRTFRPHYHAIIFNLPK----SLIQDPQKI 131
Query 198 IEKAWSYNGEPLGFAYAVPCDKGAIGYVMKYMSK--IPRIPDNMNNV--FFLSSRKDGGI 253
++ W++ G + ++ I YV+ YM+K R D + + F L S+K +
Sbjct 132 VD-TWTH-----GHIHLANNNQSTINYVVGYMTKGNFERFNDQDDRMPEFSLMSKK---M 182
Query 254 GAAYARRLMPFYRANSQCLDITVCDPYSGMSTTVTLPEYFK 294
G Y L P + + +IT +G +++P Y+K
Sbjct 183 GMGY---LTPQMKEYYRKREITCLVRENGH--IISMPRYYK 218
> Gokush_Human_feces_E_010_Microviridae_AG0123_putative.VP4
Length=305
Score = 95.5 bits (236), Expect = 8e-24, Method: Compositional matrix adjust.
Identities = 56/156 (36%), Positives = 83/156 (53%), Gaps = 18/156 (12%)
Query 85 VPCGKCILCRDKKKREWSFRATCENVFSTSIPIFVTLTYNNKHLPK-YGVFKEEIQLFMK 143
+ CG+CI CR K REW+ R E S +F+TLTY++ HLP+ + + E QLFMK
Sbjct 40 ISCGQCIGCRLSKSREWAARCVVE-ARSHKSNMFLTLTYDDAHLPEDHSLHYEHFQLFMK 98
Query 144 RLRIRLDRLGYKHQIRYFFCSEYGSKSGRPHYHAIFWNFPRDGALSNIWNVVK------- 196
R+R + + + Q+R+F C EYG K GRPHYHAI F +W++ +
Sbjct 99 RMR-KYFQTRFGQQLRFFMCGEYGDKLGRPHYHAII--FGVTFVDKQLWSIRRGNNLYRS 155
Query 197 -MIEKAWSYNGEPLGFAYAVPCDKGAIGYVMKYMSK 231
+E+ W P GF+ + YV +Y++K
Sbjct 156 ATLERLW-----PFGFSSIGAVNFETAAYVARYVTK 186
> Gokush_Human_feces_C_014_Microviridae_AG0151_putative.VP4
Length=331
Score = 93.6 bits (231), Expect = 5e-23, Method: Compositional matrix adjust.
Identities = 64/198 (32%), Positives = 92/198 (46%), Gaps = 28/198 (14%)
Query 85 VPCGKCILCRDKKKREWSFRATCENVFSTSIPIFVTLTYNNKHLPKYG------------ 132
+PCGKC CR + +W+ R E ++ IFVT+TY+ +H+P YG
Sbjct 46 LPCGKCEYCRKQMADQWATRIELEAKRWDNV-IFVTMTYDEEHIP-YGEILKGYQSIQSQ 103
Query 133 -VFKEEIQLFMKRLRIRLDRLGYKHQIRYFFCSEYGSKSGRPHYHAIFWNF-PRDGALSN 190
V K ++QLF+KRLR YK I+YF EYG ++ RPHYH IF+ P DG
Sbjct 104 TVSKRDVQLFLKRLR-----KAYKKPIKYFIAGEYGDRTKRPHYHGIFFGLKPEDGVWYK 158
Query 191 IWNVVKMIEKAWSYNGEPLGFAYAVPCDKGAIGYVMKYMSKIPRIPDNMNNVFFLSSRK- 249
+ W N GF P G+ YV +Y++K I + +++ R+
Sbjct 159 NQKGNAYFKSEWLTNIWGKGFVDFSPAAPGSYAYVAQYVNK-KAIGAEQSAKYWMQGREP 217
Query 250 -----DGGIGAAYARRLM 262
GIG Y + M
Sbjct 218 EFRIMSKGIGEEYLKEHM 235
> Gokush_gi|47566147|ref|YP_022485.1|_nonstructural_protein_[Chlamydia_phage_3]
Length=315
Score = 93.2 bits (230), Expect = 7e-23, Method: Compositional matrix adjust.
Identities = 83/291 (29%), Positives = 134/291 (46%), Gaps = 51/291 (18%)
Query 82 FMAVPCGKCILCRDKKKREWSFRATCE-NVFSTSIPIFVTLTYNNKHLPKYG-VFKEEIQ 139
++ PC KC CR + + WS+R E +++S + F+TLTY ++HLP+ G + ++ +
Sbjct 50 WVVKPCLKCRFCRVQNAKIWSYRCMHEASLYSQNC--FLTLTYEDRHLPENGSLVRDHPR 107
Query 140 LFMKRLRIRLDRLGYKHQIRYFFCSEYGSKSGRPHYHAIFWN--FPRDGALS----NIWN 193
LF++RLR + Y H+IRYF C EYGSK RPHYH + +N FP LS N
Sbjct 108 LFLRRLREHI----YPHKIRYFGCGEYGSKLQRPHYHLLIYNYDFPDKKLLSKKRGNPLF 163
Query 194 VVKMIEKAWSYNGEPLGFAYAVPCDKGAIGYVMKY-MSKI----------PRIPDNMNNV 242
V + + + W P GF+ + + GYV +Y + K+ R+P+
Sbjct 164 VSEKLMQLW-----PFGFSTVGSVTRQSAGYVARYSLKKVNGDSSQDHYGQRLPE----- 213
Query 243 FFLSSRKDGGIGAAYARRLMPFYRANSQCLDITVCDPYSGMSTTVTLPEYFKRLYY---P 299
FL GIGA + + Y+ + D V G S P Y+ +L+ P
Sbjct 214 -FLMCSLKPGIGADWYEK----YKRDVYPQDYLVVQD-KGKSFKTRPPRYYDKLHSRFDP 267
Query 300 ANSSVLSKEVRDAFKKLCDCISDRYTIHCAGNYTDKLRFSDIEKKVLRKYS 350
+ + + F L + D+ + + F+D K++ R Y
Sbjct 268 EEMEEIKQRRVEKFMALPELTQDKAEV-------KQYIFNDRTKRLFRDYE 311
Lambda K H a alpha
0.325 0.140 0.448 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 40407464