bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-4_CDS_annotation_glimmer3.pl_2_8
Length=104
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4 204 2e-65
Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4 39.7 1e-06
Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4 32.7 3e-04
Alpavirinae_Human_feces_A_021_Microviridae_AG075_putative.VP4 31.2 0.001
Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4 31.2 0.001
Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4 29.6 0.004
Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4 28.1 0.013
Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4 26.9 0.028
Pichovirinae_59_Coral_002_Microviridae_AG0338_putative.VP4 25.8 0.070
Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4 24.3 0.25
> Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4
Length=563
Score = 204 bits (518), Expect = 2e-65, Method: Compositional matrix adjust.
Identities = 101/104 (97%), Positives = 103/104 (99%), Gaps = 0/104 (0%)
Query 1 MLEHLRLLEADRYKALALRYPNFISKFRPFILRSIPRVSKLQNFKDEYFEELVWMLPEIA 60
MLEHLRLLEADRYKALALRYPNFISK RP+ILRSIPRVSKLQNFKDEYFEELVWMLPEIA
Sbjct 126 MLEHLRLLEADRYKALALRYPNFISKARPYILRSIPRVSKLQNFKDEYFEELVWMLPEIA 185
Query 61 ESLKKKNNTDASGAFPQFKGLLKYVNIRDYQLFAKRLRKYLSKK 104
ESLKKKNNTDA+GAFPQFKGLLKYVNIRDYQLFAKRLRKYLSKK
Sbjct 186 ESLKKKNNTDANGAFPQFKGLLKYVNIRDYQLFAKRLRKYLSKK 229
> Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4
Length=546
Score = 39.7 bits (91), Expect = 1e-06, Method: Compositional matrix adjust.
Identities = 17/39 (44%), Positives = 25/39 (64%), Gaps = 0/39 (0%)
Query 61 ESLKKKNNTDASGAFPQFKGLLKYVNIRDYQLFAKRLRK 99
E+ KK+ N D G +P K + Y++ +D QLF KR+RK
Sbjct 131 ENFKKQANLDVKGCYPHLKDMYGYLSRKDCQLFMKRVRK 169
> Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4
Length=532
Score = 32.7 bits (73), Expect = 3e-04, Method: Compositional matrix adjust.
Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 0/42 (0%)
Query 62 SLKKKNNTDASGAFPQFKGLLKYVNIRDYQLFAKRLRKYLSK 103
S +K N +G +P G + Y+ D L+ KR+RKY+SK
Sbjct 137 SYAQKANLSFNGKYPALSGRIPYLLHGDVSLYMKRVRKYISK 178
> Alpavirinae_Human_feces_A_021_Microviridae_AG075_putative.VP4
Length=547
Score = 31.2 bits (69), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 16/40 (40%), Positives = 22/40 (55%), Gaps = 0/40 (0%)
Query 65 KKNNTDASGAFPQFKGLLKYVNIRDYQLFAKRLRKYLSKK 104
+K N A+G +P +K Y++ D LF KRLR KK
Sbjct 145 RKVNLTANGKYPMYKDCFPYLSRYDVALFMKRLRNLFLKK 184
> Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4
Length=536
Score = 31.2 bits (69), Expect = 0.001, Method: Compositional matrix adjust.
Identities = 21/69 (30%), Positives = 32/69 (46%), Gaps = 8/69 (12%)
Query 43 NFKDEYFEELVWMLPEIAESLKKKNNTD--------ASGAFPQFKGLLKYVNIRDYQLFA 94
+F DE F+ + E +L K + D + +P LL Y+N RD QLF
Sbjct 107 SFCDEEFDYPTNLRDEAVTALLDKTHLDRTVYPDGRSVVKYPNMGDLLPYLNYRDVQLFH 166
Query 95 KRLRKYLSK 103
KR+ + + K
Sbjct 167 KRINQQIKK 175
> Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4
Length=532
Score = 29.6 bits (65), Expect = 0.004, Method: Compositional matrix adjust.
Identities = 15/42 (36%), Positives = 22/42 (52%), Gaps = 0/42 (0%)
Query 62 SLKKKNNTDASGAFPQFKGLLKYVNIRDYQLFAKRLRKYLSK 103
S +K N G +P + Y+ D L+ KR+RKY+SK
Sbjct 137 SYAQKANLSFDGKYPALSDRIPYLLHDDVSLYMKRVRKYISK 178
> Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4
Length=539
Score = 28.1 bits (61), Expect = 0.013, Method: Compositional matrix adjust.
Identities = 12/24 (50%), Positives = 16/24 (67%), Gaps = 0/24 (0%)
Query 76 PQFKGLLKYVNIRDYQLFAKRLRK 99
P +GL+ Y+N D QLF KRL +
Sbjct 149 PYMEGLVGYLNYHDIQLFFKRLNQ 172
> Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4
Length=562
Score = 26.9 bits (58), Expect = 0.028, Method: Compositional matrix adjust.
Identities = 25/79 (32%), Positives = 33/79 (42%), Gaps = 15/79 (19%)
Query 30 FILRSIPRVSKLQ--------NFKDEYFEELVWMLPEIAESLKKKNNTDASGAFPQFKGL 81
F RS+PR S ++ FKD + M P+ S+ K N + P
Sbjct 110 FGFRSVPRSSSVKLKSSTVERTFKDPDVKFSYPMKPKELLSILGKINHNVPNRIP----- 164
Query 82 LKYVNIRDYQLFAKRLRKY 100
Y+ RD LF KRLR Y
Sbjct 165 --YICNRDLDLFLKRLRSY 181
> Pichovirinae_59_Coral_002_Microviridae_AG0338_putative.VP4
Length=274
Score = 25.8 bits (55), Expect = 0.070, Method: Compositional matrix adjust.
Identities = 16/45 (36%), Positives = 23/45 (51%), Gaps = 8/45 (18%)
Query 63 LKKKNNTDASGAF--------PQFKGLLKYVNIRDYQLFAKRLRK 99
L ++ T +S F P + + +N RD+QLF KRLRK
Sbjct 45 LNEEAKTSSSACFITLTYENAPVSENGFRTLNKRDFQLFLKRLRK 89
> Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4
Length=305
Score = 24.3 bits (51), Expect = 0.25, Method: Compositional matrix adjust.
Identities = 18/53 (34%), Positives = 24/53 (45%), Gaps = 9/53 (17%)
Query 54 WMLPEIAESLKKKNN-----TDASGAFPQFKGLLKYVNIRDYQLFAKRLRKYL 101
W + E+ KNN T P+ G L Y + +QLF KR+RKY
Sbjct 56 WAARCVVEAKSHKNNMFLTLTYDDAHLPE-DGSLHYEH---FQLFMKRMRKYF 104
Lambda K H a alpha
0.325 0.140 0.406 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 5389302