bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-39_CDS_annotation_glimmer3.pl_2_1
Length=290
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4 476 8e-168
Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4 106 1e-27
Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4 102 5e-26
Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4 87.4 5e-21
Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4 86.3 1e-20
Alpavirinae_Human_gut_33_017_Microviridae_AG0152_putative.VP4 86.3 1e-20
Alpavirinae_Human_feces_A_021_Microviridae_AG075_putative.VP4 82.4 2e-19
Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4 79.3 2e-18
Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4 73.6 2e-16
Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4 71.2 1e-15
> Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4
Length=563
Score = 476 bits (1226), Expect = 8e-168, Method: Compositional matrix adjust.
Identities = 221/259 (85%), Positives = 246/259 (95%), Gaps = 0/259 (0%)
Query 1 MDSKVFKYFNKCENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCY 60
MD KVFKYFN+CE+P++IQNKYTGD+VKVDCG+CPYCLIKK+DR+TQKCDFVK+NH+YCY
Sbjct 1 MDKKVFKYFNRCEHPQVIQNKYTGDYVKVDCGQCPYCLIKKADRSTQKCDFVKYNHRYCY 60
Query 61 FVTLTYNSEYVPKMSLTQIDDYLTEWLPVRPPKSIGTQLVARMVMDSRVNKKIPNFVSAK 120
FVTLTYN++YVPKMSLTQI+DYL+EWLPVRPPKS GTQL ARM+ DSRVNKKIP+F+SA+
Sbjct 61 FVTLTYNTQYVPKMSLTQIEDYLSEWLPVRPPKSFGTQLTARMLTDSRVNKKIPDFMSAE 120
Query 121 VNRPYMLEHLHFIEAERYKALSLRYPNFGSKFRPYILRSILRKSPLQRFKDEYFEELVWM 180
VNRPYMLEHL +EA+RYKAL+LRYPNF SK RPYILRSI R S LQ FKDEYFEELVWM
Sbjct 121 VNRPYMLEHLRLLEADRYKALALRYPNFISKARPYILRSIPRVSKLQNFKDEYFEELVWM 180
Query 181 LPELAESLKKKNNTDANGAFPQFKGLLKYINIRDYQLFSKRLRKYLSKKIGKYEKIHSYV 240
LPE+AESLKKKNNTDANGAFPQFKGLLKY+NIRDYQLF+KRLRKYLSKK+GKYEKIHSYV
Sbjct 181 LPEIAESLKKKNNTDANGAFPQFKGLLKYVNIRDYQLFAKRLRKYLSKKVGKYEKIHSYV 240
Query 241 VSEYSPKTLRPHFHILFFF 259
VSEYSPKT RPHFHILFFF
Sbjct 241 VSEYSPKTFRPHFHILFFF 259
Score = 22.3 bits (46), Expect = 6.5, Method: Compositional matrix adjust.
Identities = 10/30 (33%), Positives = 14/30 (47%), Gaps = 2/30 (7%)
Query 261 LGRNRP--KHSTGCISELETWSCRYATCEG 288
RN P + + G +SE W C Y +G
Sbjct 397 FARNEPFEEATPGNVSEFICWWCAYNFRQG 426
> Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4
Length=536
Score = 106 bits (265), Expect = 1e-27, Method: Compositional matrix adjust.
Identities = 83/261 (32%), Positives = 123/261 (47%), Gaps = 59/261 (23%)
Query 1 MDSKVFKYFNKCENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCY 60
M + V K + C +P II+NKYTGD + V+CG C CL K+ + +C+ + + C+
Sbjct 1 MINPVVKPYCSCLHPVIIKNKYTGDPIYVECGTCEVCLSNKAIQKELRCNIQLASSRCCF 60
Query 61 FVTLTYNSEYVPKMSLTQIDDYLTEWLPVRPPKSIGTQLVARMVMDSRVNKKIPNFVSAK 120
FVTLTY +E++P +++D + L P + T V N+K
Sbjct 61 FVTLTYATEHIPVARFYKLND--SYHLCCVPRDHVYT-----YVTSQGYNRK-------- 105
Query 121 VNRPYMLEHLHFIEAERYKALSLRYPNFGSKFRPYILRSILRKSPLQRFKDEYFEELVWM 180
+ F + E YP + R + ++L K+ L R +
Sbjct 106 ---------MSFCDEE------FDYP---TNLRDEAVTALLDKTHLDR----------TV 137
Query 181 LPELAESLKKKNNTDANGAFPQFKGLLKYINIRDYQLFSKRLRKYLSKKIGKY--EKIHS 238
P+ +K +P LL Y+N RD QLF KR+ +++I KY EKI+S
Sbjct 138 YPDGRSVVK----------YPNMGDLLPYLNYRDVQLFHKRI----NQQIKKYTDEKIYS 183
Query 239 YVVSEYSPKTLRPHFHILFFF 259
Y V EY PKT RPHFH+LFFF
Sbjct 184 YTVGEYGPKTFRPHFHLLFFF 204
> Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4
Length=559
Score = 102 bits (253), Expect = 5e-26, Method: Compositional matrix adjust.
Identities = 70/255 (27%), Positives = 122/255 (48%), Gaps = 31/255 (12%)
Query 5 VFKYFNKCENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCYFVTL 64
+ K + C++ I N+Y G + VDCG+C YC+ K++ +A+ + KY YFVTL
Sbjct 7 LLKATDHCQHRSFITNRYNGARITVDCGQCDYCIHKRAQKASMRVKTAGSAFKYSYFVTL 66
Query 65 TYNSEYVPKMSLTQIDDYLTEWLPVRPPKSIGTQLVARMVMDSRVNKKIPNFVSAKVNRP 124
TY++E++P M+ + + + + G + +K IP + +
Sbjct 67 TYDNEHIPLMNCKVLHSEYEDVVGISGDIHFGDEY----------HKYIP-VSEYRCDDN 115
Query 125 YMLEHLHFIEAERYKALSLRYPNFGSKFRPYILRSILRKSPLQRFKDEYFEELVWMLPEL 184
ML H+ F + + F + + Y+ P+ KD +F + + +
Sbjct 116 SMLRHIFFEQVQGTVP-------FDREIKEYV--------PV---KDNWFLSMAAIRSFI 157
Query 185 AESLK-KKNNTDANGAFPQFKGLLKYINIRDYQLFSKRLRKYLSKKIGKYEKIHSYVVSE 243
++ K + A+ + + L+ ++N D Q + KRLRKYL +++GKYE H Y V E
Sbjct 158 YKTQSVDKTDYPASEQYGR-DNLIPFLNYVDVQNYIKRLRKYLFQQLGKYETFHFYAVGE 216
Query 244 YSPKTLRPHFHILFF 258
Y P RPH+H+L F
Sbjct 217 YGPVHFRPHYHLLLF 231
> Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4
Length=611
Score = 87.4 bits (215), Expect = 5e-21, Method: Compositional matrix adjust.
Identities = 81/265 (31%), Positives = 119/265 (45%), Gaps = 38/265 (14%)
Query 9 FNKCENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCYFVTLTYNS 68
F +C P+ I N Y+ D + CG C C++ KS+ AT + + KYCYFVTLTY
Sbjct 13 FTECLRPQRIVNPYSHDVIFAPCGHCKSCIMNKSNFATAYAMNMATHFKYCYFVTLTYKD 72
Query 69 EYVPKMSLTQIDDYLTEWLPVRPPKSIGTQLVARMVMDSRVNKKIPNFVSAKV--NRPYM 126
++P +L V + G + + ++ V+ P F++ + +R +
Sbjct 73 IFLP-------------YLSVEVVRRSGNRYLFDENFETMVSTSDPRFLTPEYYHDRDFS 119
Query 127 LEHLHFIEAERYKALSLRYPNFGSKFRPYILRSILRKSP----LQRFKDEYFEELVWM-L 181
L+ E E+ F F+ I R + KS + F DE + + M L
Sbjct 120 LDSAQ-NEVEQV---------FDVGFQS-IPRDVSVKSKGSFRFRSFDDEPLKFCIPMKL 168
Query 182 PELAESLKKKNNTDANG----AFPQF---KGLLKYINIRDYQLFSKRLRKYLSKKIGKYE 234
EL + L K N G +P K + + RD +LF KRLR+ L
Sbjct 169 TELQDILIKANGRYDYGKKKVVYPSLADCKLQIPVLQSRDIELFFKRLRRNLDSHGFTSS 228
Query 235 KIHSYVVSEYSPKTLRPHFHILFFF 259
KI YVVSEY P+T RPH+H L FF
Sbjct 229 KICYYVVSEYGPQTYRPHWHCLLFF 253
> Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4
Length=562
Score = 86.3 bits (212), Expect = 1e-20, Method: Compositional matrix adjust.
Identities = 79/285 (28%), Positives = 117/285 (41%), Gaps = 60/285 (21%)
Query 7 KYFNKCENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCYFVTLTY 66
K +C++PR + NKYT + V V CG CP C++++S T +Y YFVTLTY
Sbjct 9 KLVTRCQHPRTVVNKYTHEPVVVSCGHCPSCILRRSSVQTNLLTTYSAQFRYVYFVTLTY 68
Query 67 NSEYVPKMSLTQIDDYLTEWLPVRPPKSIGTQLVARMVMDSRVNKKIPNFVSAKVNRPYM 126
++P + ++ ++ + V +PN
Sbjct 69 APSFLPTLEVSVVETCTDDIADV---------------------SCVPN----------- 96
Query 127 LEHLHFIEAERYKALSLRYPNFGSKFRPYILRSILRKSPLQR-FKDEYFEELVWMLPELA 185
++ L + Y FG + P L+ S ++R FKD + M P+
Sbjct 97 IDELDASDPNTYL--------FGFRSVPRSSSVKLKSSTVERTFKDPDVKFSYPMKPKEL 148
Query 186 ESLKKKNNTDANGAFPQFKGLLKYINIRDYQLFSKRLRKYLSKKIGKYEKIHSYVVSEYS 245
S+ K N + P YI RD LF KRLR Y EK+ Y VSEY
Sbjct 149 LSILGKINHNVPNRIP-------YICNRDLDLFLKRLRSYYLD-----EKLRYYAVSEYG 196
Query 246 PKTLRPHFHILFFFRLGRNRPKHS-TGCISELETWSCRYATCEGQ 289
P + RPH+H+L F N + S T C + + WS Y C+
Sbjct 197 PTSFRPHWHLLLF----SNSERFSRTVCENVSKAWS--YGRCDAS 235
> Alpavirinae_Human_gut_33_017_Microviridae_AG0152_putative.VP4
Length=565
Score = 86.3 bits (212), Expect = 1e-20, Method: Compositional matrix adjust.
Identities = 75/253 (30%), Positives = 104/253 (41%), Gaps = 53/253 (21%)
Query 7 KYFNKCENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCYFVTLTY 66
K +C+NPR + NKYT + V V CG CP C++++S T +Y YFVTLTY
Sbjct 9 KLVTRCQNPRTVVNKYTHEPVVVSCGACPSCVLRRSGIQTNLLTTYSAQFRYVYFVTLTY 68
Query 67 NSEYVPKMSLTQIDDYLTEWLPVRPPKSIGTQLVARMVMDSRVNKKIPNFVSAKVNRPYM 126
++P + ++ I+ T +A + +N P
Sbjct 69 APCFLPTLEVSVIE--------------TCTDDIADVSSVPDINDLDP------------ 102
Query 127 LEHLHFIEAERYKALSLRYPNFGSKFRPYILRSILRKSPLQR-FKDEYFEELVWMLPELA 185
+ RY FG P L+ S ++R FKD M P+
Sbjct 103 ------CDNNRYL--------FGFCSVPRSASVKLKNSTVERTFKDPEVRFSYPMKPKEL 148
Query 186 ESLKKKNNTDANGAFPQFKGLLKYINIRDYQLFSKRLRKYLSKKIGKYEKIHSYVVSEYS 245
S+ K N + P YI RD LF KRLR Y YEK+ Y VSEY
Sbjct 149 LSILDKINHNVPNRIP-------YICNRDLDLFLKRLRSYY-----PYEKLRYYAVSEYG 196
Query 246 PKTLRPHFHILFF 258
P + RPH+H+L F
Sbjct 197 PTSYRPHWHLLLF 209
> Alpavirinae_Human_feces_A_021_Microviridae_AG075_putative.VP4
Length=547
Score = 82.4 bits (202), Expect = 2e-19, Method: Compositional matrix adjust.
Identities = 68/254 (27%), Positives = 106/254 (42%), Gaps = 49/254 (19%)
Query 3 SKVFKYFNKCENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCYFV 62
S F + C PR ++ ++ D + + C EC CL+KKS T KY +FV
Sbjct 7 SSRFAPMSDCNQPRYLRQLHSDDVLSIGCNECLPCLLKKSRNDTLDVTISTQTFKYIFFV 66
Query 63 TLTYNSEYVPKMSLTQIDDYLTEWLPVRPPKSIGTQLVARMVMDSRVNKKIPNFVSAKVN 122
L Y + ++P M E++P R K I + + R + NK+ + +
Sbjct 67 NLDYATPFIPIMR--------HEYIPER--KVIYCESMVRPSVKIPTNKR-------RKD 109
Query 123 RPYMLEHLHFIEAERYKALSLRYPNFGSKFRPYILRSILRKSPLQRFKDEYFEELVWMLP 182
+ F A+ + RP+ +P + +E + V
Sbjct 110 GSVVFTTFSFRAAD--------------ETRPFCF-----TAPCE--SEEEYRLFV---- 144
Query 183 ELAESLKKKNNTDANGAFPQFKGLLKYINIRDYQLFSKRLRKYLSKKIGKYEKIHSYVVS 242
+K N ANG +P +K Y++ D LF KRLR KK G+ +HSY+V
Sbjct 145 -------RKVNLTANGKYPMYKDCFPYLSRYDVALFMKRLRNLFLKKYGQSIYMHSYIVG 197
Query 243 EYSPKTLRPHFHIL 256
EY P RPH+HI+
Sbjct 198 EYGPVHFRPHYHII 211
> Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4
Length=546
Score = 79.3 bits (194), Expect = 2e-18, Method: Compositional matrix adjust.
Identities = 42/88 (48%), Positives = 53/88 (60%), Gaps = 4/88 (5%)
Query 186 ESLKKKNNTDANGAFPQFKGLLKYINIRDYQLFSKRLRKYLSKKIGKYEKIHSYVVSEYS 245
E+ KK+ N D G +P K + Y++ +D QLF KR+RK + EKIH+Y+V EYS
Sbjct 131 ENFKKQANLDVKGCYPHLKDMYGYLSRKDCQLFMKRVRKQIRNYTD--EKIHTYIVGEYS 188
Query 246 PKTLRPHFHILFFFRLGRNRPKHSTGCI 273
PK RPHFHILFFF N S G I
Sbjct 189 PKHFRPHFHILFFF--NSNELSQSFGSI 214
Score = 45.8 bits (107), Expect = 2e-07, Method: Compositional matrix adjust.
Identities = 23/62 (37%), Positives = 34/62 (55%), Gaps = 0/62 (0%)
Query 12 CENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCYFVTLTYNSEYV 71
C + + + NKYTG + CG+C CL + + + K KY FVTLTY++ +V
Sbjct 15 CLHGKNVYNKYTGQMMYQSCGKCEACLSRLASARSIKVGVQASLSKYVMFVTLTYDTYHV 74
Query 72 PK 73
PK
Sbjct 75 PK 76
> Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4
Length=532
Score = 73.6 bits (179), Expect = 2e-16, Method: Compositional matrix adjust.
Identities = 40/107 (37%), Positives = 56/107 (52%), Gaps = 10/107 (9%)
Query 187 SLKKKNNTDANGAFPQFKGLLKYINIRDYQLFSKRLRKYLSKKIGKYEKIHSYVVSEYSP 246
S +K N NG +P G + Y+ D L+ KR+RKY+SK +G E IH+Y+V EY P
Sbjct 137 SYAQKANLSFNGKYPALSGRIPYLLHGDVSLYMKRVRKYISK-LGINETIHTYIVGEYGP 195
Query 247 KTLRPHFHILFFF---RLGRNRPKHSTGCISELETWSCRYATCEGQR 290
+ RPHFH+L FF L +N + ++ C W C R
Sbjct 196 SSFRPHFHLLLFFDSDELAQNIIRIASSC------WRFGRVDCSASR 236
Score = 54.7 bits (130), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 25/66 (38%), Positives = 35/66 (53%), Gaps = 0/66 (0%)
Query 7 KYFNKCENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCYFVTLTY 66
K F+ C++ +N YTG+ + V CG CP C KS + K ++ YFVTLTY
Sbjct 9 KVFSMCQHQVQTKNPYTGELIMVPCGTCPACRFNKSILSQNKVHAQSLVSRHVYFVTLTY 68
Query 67 NSEYVP 72
Y+P
Sbjct 69 AQRYIP 74
> Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4
Length=539
Score = 71.2 bits (173), Expect = 1e-15, Method: Compositional matrix adjust.
Identities = 30/66 (45%), Positives = 46/66 (70%), Gaps = 0/66 (0%)
Query 7 KYFNKCENPRIIQNKYTGDFVKVDCGECPYCLIKKSDRATQKCDFVKFNHKYCYFVTLTY 66
K + C +PR+I+NKYTGD + V CG C +C+ K+ +A KC+ + +YC F+TLTY
Sbjct 7 KTYGSCLHPRVIKNKYTGDPIYVPCGTCEFCVHNKAIKAELKCNVQLASSRYCEFITLTY 66
Query 67 NSEYVP 72
++EY+P
Sbjct 67 STEYLP 72
Score = 60.5 bits (145), Expect = 4e-12, Method: Compositional matrix adjust.
Identities = 30/58 (52%), Positives = 37/58 (64%), Gaps = 2/58 (3%)
Query 201 PQFKGLLKYINIRDYQLFSKRLRKYLSKKIGKYEKIHSYVVSEYSPKTLRPHFHILFF 258
P +GL+ Y+N D QLF KRL + + + EKI+ YVV EY P T RPHFHIL F
Sbjct 149 PYMEGLVGYLNYHDIQLFFKRLNQNIRRITN--EKIYYYVVGEYGPTTFRPHFHILLF 204
Lambda K H a alpha
0.324 0.139 0.433 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 24051702