bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-32_CDS_annotation_glimmer3.pl_2_1
Length=229
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 397 7e-141
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 396 1e-140
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 391 1e-138
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 375 2e-132
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 374 2e-132
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 247 9e-83
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 206 5e-66
Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 200 4e-64
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 196 1e-62
Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 191 3e-60
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 397 bits (1019), Expect = 7e-141, Method: Compositional matrix adjust.
Identities = 189/221 (86%), Positives = 205/221 (93%), Gaps = 0/221 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
+ECR+QKQR+WLVRMSEELR PNAYFMTLTISDENYEILKNICKSED NTIATKAIRLM
Sbjct 42 LECRKQKQREWLVRMSEELRTEPNAYFMTLTISDENYEILKNICKSEDDNTIATKAIRLM 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIG D+LIEEKWNYGIT+TG +VNE
Sbjct 102 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDQLIEEKWNYGITYTGNYVNE 161
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
+TINY+TKYMTK D HP F+GKVLCS+GIG GY KR DA H YKKG+TIE YRLRNG+
Sbjct 162 KTINYVTKYMTKIDEKHPDFVGKVLCSRGIGAGYTKRPDAAKHKYKKGETIETYRLRNGA 221
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRD 221
KINLPIYYRN+LFTE+ERE+LF+DKIEKGIIYVMGQKVHRD
Sbjct 222 KINLPIYYRNKLFTEKERELLFIDKIEKGIIYVMGQKVHRD 262
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 396 bits (1018), Expect = 1e-140, Method: Compositional matrix adjust.
Identities = 189/221 (86%), Positives = 198/221 (90%), Gaps = 0/221 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
MECRQQKQRQWLVRMSEELRQ PNAYFMTLTI DENY L NIC S+D N IATKA+RLM
Sbjct 42 MECRQQKQRQWLVRMSEELRQNPNAYFMTLTIDDENYNKLANICNSKDNNEIATKAVRLM 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIG D+LI EKWNYG +TG FVNE
Sbjct 102 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDQLISEKWNYGFVYTGNFVNE 161
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
TINYITKYMTK D+DHP F+G+VLCSKGIG GY KREDA NH Y KGKT E YRLRNG+
Sbjct 162 ATINYITKYMTKVDIDHPDFVGQVLCSKGIGAGYTKREDANNHKYTKGKTNETYRLRNGA 221
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRD 221
KINLPIYYRNQLF+EEEREMLFLDKIEKGIIYVMGQKVHRD
Sbjct 222 KINLPIYYRNQLFSEEEREMLFLDKIEKGIIYVMGQKVHRD 262
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 391 bits (1004), Expect = 1e-138, Method: Compositional matrix adjust.
Identities = 188/229 (82%), Positives = 206/229 (90%), Gaps = 0/229 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
+ECR+QKQR+WLVRMSEELR PNAYFMTLTISDENYEILKNICKSED NTIATKAIRLM
Sbjct 42 LECRKQKQREWLVRMSEELRTEPNAYFMTLTISDENYEILKNICKSEDENTIATKAIRLM 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRKK GKSI+HWFITELGHEKTERLHLHGIVWGIG D+LI EKWNYGIT+TG FVNE
Sbjct 102 LERIRKKIGKSIRHWFITELGHEKTERLHLHGIVWGIGTDQLIREKWNYGITYTGNFVNE 161
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
+TINYITKYMTK D +HP F+GKVLCSKGIG GY KR DA H YKKG+TIE YRLRNG+
Sbjct 162 KTINYITKYMTKIDEEHPNFVGKVLCSKGIGAGYTKRPDAAKHKYKKGETIETYRLRNGA 221
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRDIRKRKISM 229
KINLPIYYRN+LFTE+ERE+LF+DKI+KGIIYV+G KVHRD K I +
Sbjct 222 KINLPIYYRNKLFTEKERELLFIDKIDKGIIYVLGTKVHRDDEKYYIQL 270
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 375 bits (963), Expect = 2e-132, Method: Compositional matrix adjust.
Identities = 184/221 (83%), Positives = 193/221 (87%), Gaps = 0/221 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
MECRQQKQRQWLVRMSEELRQ PNAYF+TLTI D++Y L N D N IATKAIRL
Sbjct 42 MECRQQKQRQWLVRMSEELRQEPNAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLC 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRK+TGKSIKHWFITELGHEKTERLHLHGIVWGIG DKLI KWNYGITFTG+FVNE
Sbjct 102 LERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDKLITSKWNYGITFTGFFVNE 161
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
+TI YITKYMTK D H FIGKVLCSKGIG GY KR+DAK H YK+G+TIE YRLRNGS
Sbjct 162 KTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKRGETIETYRLRNGS 221
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRD 221
KINLPIYYRNQLFTEEERE LFLDKIEKGIIYVMGQKVHRD
Sbjct 222 KINLPIYYRNQLFTEEEREALFLDKIEKGIIYVMGQKVHRD 262
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 374 bits (959), Expect = 2e-132, Method: Compositional matrix adjust.
Identities = 183/221 (83%), Positives = 192/221 (87%), Gaps = 0/221 (0%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
MECRQQKQRQWLVRMSEELRQ PNAYF+TLTI D++Y L N D N IATKAIRL
Sbjct 1 MECRQQKQRQWLVRMSEELRQEPNAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLC 60
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNE 120
LERIRK+TGKSIKHWFITELGHEKTERLHLHGIVWGIG DKLI KWNYGITFTG+FVNE
Sbjct 61 LERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDKLITNKWNYGITFTGFFVNE 120
Query 121 QTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGS 180
+TI YITKYMTK D H FIGKVLCSKGIG GY KR+DAK H YK G+TIE YRLRNGS
Sbjct 121 KTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKPGETIETYRLRNGS 180
Query 181 KINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRD 221
KINLPIYYRNQLFTEEE+E LFLDKIEKGIIYVMGQKVHRD
Sbjct 181 KINLPIYYRNQLFTEEEKEALFLDKIEKGIIYVMGQKVHRD 221
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 247 bits (631), Expect = 9e-83, Method: Compositional matrix adjust.
Identities = 119/218 (55%), Positives = 154/218 (71%), Gaps = 0/218 (0%)
Query 2 ECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLML 61
ECR+QK R W VR+SEE+R PNA F+TLTISDE++E +KN K +RL L
Sbjct 43 ECRKQKGRAWQVRLSEEVRSDPNAIFVTLTISDESWEKIKNTYIQLSDEDCIKKMVRLFL 102
Query 62 ERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVNEQ 121
ER+RKKT KS+KHW TE G TER HLHG++WG + L + W YG F G FVNE
Sbjct 103 ERVRKKTKKSLKHWLTTERGGTNTERYHLHGLIWGENTEALTKSLWQYGFVFIGTFVNEC 162
Query 122 TINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNGSK 181
T+NYITKY+TK D H F LCS GIG GY R D++ + +++GKT E YRLRNG+K
Sbjct 163 TVNYITKYITKTDKKHKDFEPITLCSAGIGKGYLSRSDSELNRFREGKTTETYRLRNGTK 222
Query 182 INLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVH 219
+NLPIYYRN+L+T+EERE LFL+KI+KG +++ G++ +
Sbjct 223 LNLPIYYRNKLYTDEEREKLFLEKIKKGKVWICGRECN 260
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 206 bits (525), Expect = 5e-66, Method: Compositional matrix adjust.
Identities = 108/228 (47%), Positives = 154/228 (68%), Gaps = 12/228 (5%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICK---SEDPNTIATKAI 57
+ECR++KQR+W VR+ EE+ + YF TLTI EN + +++I E+PN IATKA+
Sbjct 42 IECRKEKQREWRVRLEEEM-HSNFGYFTTLTIDQENIKKIESITGLKWEENPNEIATKAL 100
Query 58 RLMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYF 117
RL LER RK TGKSI+HW +TELG EK R+HLHGI +G +LI + WNYG F G +
Sbjct 101 RLFLERTRKDTGKSIRHWCVTELG-EKNNRIHLHGIFFGQKSAELIRKHWNYGFIFIGGY 159
Query 118 VNEQTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGK----TIEY 173
N ++INYITKYM K D+ HP F VL S GIG GY R D ++++K +
Sbjct 160 CNSKSINYITKYMLKVDIKHPEFKQIVLASSGIGKGYIDRLD---YLWQKQNYKNINVAT 216
Query 174 YRLRNGSKINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRD 221
Y RNG+K+ +P YY+N++FTE+ERE ++++ + +G++++ G+KV D
Sbjct 217 YTFRNGTKMAMPKYYKNKIFTEKEREKMWINNLNRGLLWIYGEKVKAD 264
> Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4
Length=316
Score = 200 bits (508), Expect = 4e-64, Method: Compositional matrix adjust.
Identities = 111/222 (50%), Positives = 147/222 (66%), Gaps = 7/222 (3%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNA-YFMTLTISDENYEILKNICKSEDPNTIATKAIRL 59
+ECRQQKQR W+VR+SEELR A F+TLT ++E+Y+ L I K+E N + A+
Sbjct 42 IECRQQKQRAWIVRLSEELRSGKGAGLFVTLTFNEESYKELAAITKNE--NDMCRLALYR 99
Query 60 MLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGITFTGYFVN 119
M E R+K +I+HW +TE+G + R+H+HGI+W D IE W YG + G FVN
Sbjct 100 MNENYRQKYKHTIRHWCVTEIGDDG--RIHIHGIMWCPASD--IERYWKYGYIYIGRFVN 155
Query 120 EQTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYYRLRNG 179
EQTI YITKYM K F KVLCSKGIG+ Y R D+K + Y++ T E Y LR+G
Sbjct 156 EQTILYITKYMLKYTPVDKNFEPKVLCSKGIGINYLDRLDSKRNTYRENNTDESYMLRSG 215
Query 180 SKINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRD 221
KINLP YY+ +++TEEERE L+++K EKG Y+MG+KV D
Sbjct 216 RKINLPDYYKRKIYTEEEREKLWIEKQEKGYRYIMGEKVSTD 257
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 196 bits (497), Expect = 1e-62, Method: Compositional matrix adjust.
Identities = 107/229 (47%), Positives = 146/229 (64%), Gaps = 12/229 (5%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEIL--------KNICKSEDPNTI 52
+ECR++K R W +R++EEL+ PNA F+TLT ++ENY+ L K+ + N +
Sbjct 40 IECRKEKARNWRIRLAEELKNNPNALFITLTFNEENYQRLSWELFKKSKDNLNYTEQNEM 99
Query 53 ATKAIRLMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGIDKLIEEKWNYGIT 112
A+R LERIRKKT KSI+HW +TE G E R+HLHGIVW K E+W YG T
Sbjct 100 CKTAVRRWLERIRKKTKKSIRHWMVTEKG-EDYGRIHLHGIVW---CPKERIEQWGYGYT 155
Query 113 FTGYFVNEQTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIE 172
+ G +VNE TI Y+TKYM K P F GKV+CS GIG Y +AK + Y+ +T E
Sbjct 156 YIGDYVNETTIAYVTKYMLKICEKWPDFRGKVMCSAGIGCRYETSYNAKRNRYRGKETKE 215
Query 173 YYRLRNGSKINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKVHRD 221
Y+L NG ++ LP YY ++++TEEERE L++ K E+G Y+ G+KV D
Sbjct 216 TYKLENGRELPLPKYYHDKIYTEEEREKLWIIKQERGYRYIAGEKVSTD 264
> Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4
Length=355
Score = 191 bits (485), Expect = 3e-60, Method: Compositional matrix adjust.
Identities = 106/224 (47%), Positives = 142/224 (63%), Gaps = 7/224 (3%)
Query 1 MECRQQKQRQWLVRMSEELRQTPNAYFMTLTISDENYEILKNICKSEDPNTIATKAIRLM 60
ECR++K+ W VR E+LR+TP A F T T+S E Y+ +K + N I TK RL
Sbjct 42 FECRKKKRNAWRVRNFEQLRETPTAIFFTGTVSPERYDYIKEKYNLKTDNEIITKIHRLF 101
Query 61 LERIRKKTGKSIKHWFITELGHEKTERLHLHGIVW---GIGIDKLIE---EKWNYGITFT 114
LERIRK+TGKS+KHW +TE GH T R+HLHGI + G+ KLI W G +
Sbjct 102 LERIRKETGKSMKHWCVTEKGHTNTRRIHLHGIFYAPNGMTQFKLINILRNNWIDGYCYN 161
Query 115 GYFVNEQTINYITKYMTKEDLDHPGFIGKVLCSKGIGMGYAKREDAKNHVYKKGKTIEYY 174
G + NE+TINY++KYMTK+D+D+P + GKVLCS G+G GY KR K H + + T E Y
Sbjct 162 GKYCNEKTINYVSKYMTKKDMDNPEYTGKVLCSPGLGAGYVKR-IGKRHEWNEENTKEDY 220
Query 175 RLRNGSKINLPIYYRNQLFTEEEREMLFLDKIEKGIIYVMGQKV 218
R G+ I LP YY+ +LFTE++RE L++ + G +V KV
Sbjct 221 YTRQGTYIALPKYYKYKLFTEDQREQLWIYRENSGEKFVGNFKV 264
Lambda K H a alpha
0.321 0.138 0.414 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 17847033