bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-31_CDS_annotation_glimmer3.pl_2_6
Length=236
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4 189 2e-57
Alpavirinae_Human_gut_33_017_Microviridae_AG0152_putative.VP4 123 5e-34
Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4 119 2e-32
Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4 93.2 2e-23
Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4 82.4 9e-20
Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4 81.6 2e-19
Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4 79.7 7e-19
Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4 78.2 2e-18
Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4 77.0 5e-18
Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4 67.8 5e-15
> Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4
Length=611
Score = 189 bits (479), Expect = 2e-57, Method: Compositional matrix adjust.
Identities = 101/154 (66%), Positives = 120/154 (78%), Gaps = 0/154 (0%)
Query 6 IKDILRRANGKYSYSLRKVVYPPASEWKLQIPVIRNRDFELFMKRLRSNLKQQGLPHEEI 65
++DIL +ANG+Y Y +KVVYP ++ KLQIPV+++RD ELF KRLR NL G +I
Sbjct 171 LQDILIKANGRYDYGKKKVVYPSLADCKLQIPVLQSRDIELFFKRLRRNLDSHGFTSSKI 230
Query 66 RYYCVSEYGPQTLRPHWHLLLFFDSPQLTQAIRENVCKAWSYGNCDVSLSRGaaasyvas 125
YY VSEYGPQT RPHWH LLFF+S ++TQ +RE++ KAWSYG D SLSRGAAASYVA+
Sbjct 231 CYYVVSEYGPQTYRPHWHCLLFFNSEEITQTLREDISKAWSYGRIDYSLSRGAAASYVAA 290
Query 126 yvnsvasLPYLYTGHKEIRPRCFHSKGFGQNKSF 159
YVNS A LP+LY G KEIRPR FHSKGFG NK F
Sbjct 291 YVNSAACLPFLYVGQKEIRPRSFHSKGFGSNKVF 324
Score = 22.3 bits (46), Expect = 4.5, Method: Compositional matrix adjust.
Identities = 12/27 (44%), Positives = 15/27 (56%), Gaps = 4/27 (15%)
Query 82 WHLLLFFDSPQLTQAIRENVCKAWSYG 108
W+L + DS L AI+ K WSYG
Sbjct 487 WNLKQYTDSWSLKVAIK----KIWSYG 509
> Alpavirinae_Human_gut_33_017_Microviridae_AG0152_putative.VP4
Length=565
Score = 123 bits (309), Expect = 5e-34, Method: Compositional matrix adjust.
Identities = 74/126 (59%), Positives = 90/126 (71%), Gaps = 6/126 (5%)
Query 35 QIPVIRNRDFELFMKRLRSNLKQQGLPHEEIRYYCVSEYGPQTLRPHWHLLLFFDSPQLT 94
+IP I NRD +LF+KRLRS P+E++RYY VSEYGP + RPHWHLLLF +S Q +
Sbjct 162 RIPYICNRDLDLFLKRLRSYY-----PYEKLRYYAVSEYGPTSYRPHWHLLLFSNSEQFS 216
Query 95 QAIRENVCKAWSYGNCDVSLSRGaaasyvasyvnsvasLPYLYTGH-KEIRPRCFHSKGF 153
+ I ENV KAWSYG CD SLSRG AA YVASYVNS +LP YT + +RP+ FHS GF
Sbjct 217 KTILENVSKAWSYGRCDASLSRGFAAPYVASYVNSFVALPSFYTEMPRFLRPKSFHSIGF 276
Query 154 GQNKSF 159
++ F
Sbjct 277 TESNLF 282
> Alpavirinae_Human_gut_22_017_Microviridae_AG0393_putative.VP4
Length=562
Score = 119 bits (297), Expect = 2e-32, Method: Compositional matrix adjust.
Identities = 72/126 (57%), Positives = 88/126 (70%), Gaps = 6/126 (5%)
Query 35 QIPVIRNRDFELFMKRLRSNLKQQGLPHEEIRYYCVSEYGPQTLRPHWHLLLFFDSPQLT 94
+IP I NRD +LF+KRLRS E++RYY VSEYGP + RPHWHLLLF +S + +
Sbjct 162 RIPYICNRDLDLFLKRLRSYYLD-----EKLRYYAVSEYGPTSFRPHWHLLLFSNSERFS 216
Query 95 QAIRENVCKAWSYGNCDVSLSRGaaasyvasyvnsvasLPYLYTGH-KEIRPRCFHSKGF 153
+ + ENV KAWSYG CD SLSRG AA YVASYVNS +LP YT K +RP+ FHS GF
Sbjct 217 RTVCENVSKAWSYGRCDASLSRGFAAPYVASYVNSFVALPDFYTQMPKVVRPKSFHSIGF 276
Query 154 GQNKSF 159
++ F
Sbjct 277 TESNLF 282
> Alpavirinae_Human_feces_D_015_Microviridae_AG020_putative.VP4
Length=563
Score = 93.2 bits (230), Expect = 2e-23, Method: Compositional matrix adjust.
Identities = 53/113 (47%), Positives = 75/113 (66%), Gaps = 0/113 (0%)
Query 42 RDFELFMKRLRSNLKQQGLPHEEIRYYCVSEYGPQTLRPHWHLLLFFDSPQLTQAIRENV 101
RD++LF KRLR L ++ +E+I Y VSEY P+T RPH+H+L FFDS ++ + R+ V
Sbjct 213 RDYQLFAKRLRKYLSKKVGKYEKIHSYVVSEYSPKTFRPHFHILFFFDSDEIAKNFRQAV 272
Query 102 CKAWSYGNCDVSLSRGaaasyvasyvnsvasLPYLYTGHKEIRPRCFHSKGFG 154
++W G D L+R A SYV++Y+NSV S+P++Y K IRPR S FG
Sbjct 273 YQSWRLGRVDTQLAREQANSYVSNYLNSVVSIPFVYKAKKSIRPRSRFSNLFG 325
> Alpavirinae_Human_feces_D_008_Microviridae_AG0100_putative.VP4
Length=532
Score = 82.4 bits (202), Expect = 9e-20, Method: Compositional matrix adjust.
Identities = 56/129 (43%), Positives = 76/129 (59%), Gaps = 4/129 (3%)
Query 26 YPPASEWKLQIPVIRNRDFELFMKRLRSNLKQQGLPHEEIRYYCVSEYGPQTLRPHWHLL 85
YP S+ +IP + + D L+MKR+R + + G+ +E I Y V EYGP T RPH+HLL
Sbjct 150 YPALSD---RIPYLLHDDVSLYMKRVRKYISKLGI-NETIHTYVVGEYGPVTFRPHFHLL 205
Query 86 LFFDSPQLTQAIRENVCKAWSYGNCDVSLSRGaaasyvasyvnsvasLPYLYTGHKEIRP 145
LFF+S +L Q+I W +G D S SRG A YV+SY+NS +S+P + IRP
Sbjct 206 LFFNSDELAQSIVRIARSCWRFGRVDCSASRGDAEDYVSSYLNSFSSIPLHIQEIRAIRP 265
Query 146 RCFHSKGFG 154
S FG
Sbjct 266 FARFSNKFG 274
> Alpavirinae_Human_feces_D_031_Microviridae_AG0423_putative.VP4
Length=532
Score = 81.6 bits (200), Expect = 2e-19, Method: Compositional matrix adjust.
Identities = 53/120 (44%), Positives = 71/120 (59%), Gaps = 1/120 (1%)
Query 35 QIPVIRNRDFELFMKRLRSNLKQQGLPHEEIRYYCVSEYGPQTLRPHWHLLLFFDSPQLT 94
+IP + + D L+MKR+R + + G+ +E I Y V EYGP + RPH+HLLLFFDS +L
Sbjct 156 RIPYLLHGDVSLYMKRVRKYISKLGI-NETIHTYIVGEYGPSSFRPHFHLLLFFDSDELA 214
Query 95 QAIRENVCKAWSYGNCDVSLSRGaaasyvasyvnsvasLPYLYTGHKEIRPRCFHSKGFG 154
Q I W +G D S SRG A YV+SY+NS +S+P + IRP S FG
Sbjct 215 QNIIRIASSCWRFGRVDCSASRGDAEDYVSSYLNSFSSIPLHIQEIRAIRPFARFSNKFG 274
> Alpavirinae_Human_gut_33_005_Microviridae_AG0180_putative.VP4
Length=536
Score = 79.7 bits (195), Expect = 7e-19, Method: Compositional matrix adjust.
Identities = 55/135 (41%), Positives = 78/135 (58%), Gaps = 10/135 (7%)
Query 36 IPVIRNRDFELFMKRLRSNLKQQGLPHEEIRYYCVSEYGPQTLRPHWHLLLFFDSPQLTQ 95
+P + RD +LF KR+ +K+ E+I Y V EYGP+T RPH+HLL FFDS +L Q
Sbjct 154 LPYLNYRDVQLFHKRINQQIKK--YTDEKIYSYTVGEYGPKTFRPHFHLLFFFDSERLAQ 211
Query 96 AIRENVCKAWSYGNCDVSLSRGaaasyvasyvnsvasLPYLYTGHKEIRPRCFHSKGFGQ 155
+ V KAW +GN D +A+SYVA Y+NS LP Y +++I P FG+
Sbjct 212 VFGQLVDKAWRFGNSDTQRVWSSASSYVAGYLNSSHCLPEFYRCNRKIAP-------FGR 264
Query 156 -NKSFVNRPVFQKFE 169
++ F RP + F+
Sbjct 265 FSQYFAERPFIEAFK 279
> Alpavirinae_Human_feces_A_034_Microviridae_AG0102_putative.VP4
Length=539
Score = 78.2 bits (191), Expect = 2e-18, Method: Compositional matrix adjust.
Identities = 49/114 (43%), Positives = 72/114 (63%), Gaps = 2/114 (2%)
Query 43 DFELFMKRLRSNLKQQGLPHEEIRYYCVSEYGPQTLRPHWHLLLFFDSPQLTQAIRENVC 102
D +LF KRL N+++ + +E+I YY V EYGP T RPH+H+LLF DS +L ++IR+ V
Sbjct 162 DIQLFFKRLNQNIRR--ITNEKIYYYVVGEYGPTTFRPHFHILLFHDSRKLRESIRQFVS 219
Query 103 KAWSYGNCDVSLSRGaaasyvasyvnsvasLPYLYTGHKEIRPRCFHSKGFGQN 156
K+W +G+ D +A+ YVA YVNS A LP + + I+P S F ++
Sbjct 220 KSWRFGDSDTQPVWSSASCYVAGYVNSTACLPDFFKNSRHIKPFGRFSVNFAES 273
> Alpavirinae_Human_feces_B_039_Microviridae_AG093_putative.VP4
Length=559
Score = 77.0 bits (188), Expect = 5e-18, Method: Compositional matrix adjust.
Identities = 56/136 (41%), Positives = 77/136 (57%), Gaps = 2/136 (1%)
Query 20 SLRKVVYPPASEWKLQ--IPVIRNRDFELFMKRLRSNLKQQGLPHEEIRYYCVSEYGPQT 77
S+ K YP + ++ IP + D + ++KRLR L QQ +E +Y V EYGP
Sbjct 162 SVDKTDYPASEQYGRDNLIPFLNYVDVQNYIKRLRKYLFQQLGKYETFHFYAVGEYGPVH 221
Query 78 LRPHWHLLLFFDSPQLTQAIRENVCKAWSYGNCDVSLSRGaaasyvasyvnsvasLPYLY 137
RPH+HLLLF +S ++++ +R K+W G D S G A SYVASYVNS+ S P LY
Sbjct 222 FRPHYHLLLFTNSDKVSEVLRYCHDKSWKLGRSDFQRSAGGAGSYVASYVNSLCSAPLLY 281
Query 138 TGHKEIRPRCFHSKGF 153
+ RP+ S GF
Sbjct 282 RSCRAFRPKSRASVGF 297
> Alpavirinae_Human_feces_C_043_Microviridae_AG0321_putative.VP4
Length=546
Score = 67.8 bits (164), Expect = 5e-15, Method: Compositional matrix adjust.
Identities = 47/104 (45%), Positives = 62/104 (60%), Gaps = 2/104 (2%)
Query 42 RDFELFMKRLRSNLKQQGLPHEEIRYYCVSEYGPQTLRPHWHLLLFFDSPQLTQAIRENV 101
+D +LFMKR+R ++ E+I Y V EY P+ RPH+H+L FF+S +L+Q+ V
Sbjct 158 KDCQLFMKRVRKQIR--NYTDEKIHTYIVGEYSPKHFRPHFHILFFFNSNELSQSFGSIV 215
Query 102 CKAWSYGNCDVSLSRGaaasyvasyvnsvasLPYLYTGHKEIRP 145
W +G D S SRG A SYVA YVNS A LPY +I P
Sbjct 216 RSCWKFGLVDWSQSRGDAESYVAGYVNSFARLPYHLGSSPKIAP 259
Lambda K H a alpha
0.326 0.141 0.441 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 18613470