bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-23_CDS_annotation_glimmer3.pl_2_4
Length=298
Score E
Sequences producing significant alignments: (Bits) Value
Gokush_Human_feces_E_007_Microviridae_AG0414_putative.VP4 554 0.0
Gokush_Human_gut_33_003_Microviridae_AG066_putative.VP4 523 0.0
Gokush_Human_feces_A_019_Microviridae_AG0436_putative.VP4 290 5e-98
Gokush_Human_feces_E_017_Microviridae_AG0124_putative.VP4 289 2e-97
Gokush_Human_feces_A_020_Microviridae_AG0262_putative.VP4 288 3e-97
Gokush_Human_feces_B_029_Microviridae_AG0416_putative.VP4 237 8e-78
Gokush_Human_feces_C_014_Microviridae_AG0151_putative.VP4 181 1e-55
Gokush_Human_gut_32_030_Microviridae_AG0185_putative.VP4 158 3e-47
Gokush_Human_gut_31_045_Microviridae_AG0114_putative.VP4 158 3e-47
Gokush_Human_gut_27_035_Microviridae_AG0163_putative.VP4 158 3e-47
> Gokush_Human_feces_E_007_Microviridae_AG0414_putative.VP4
Length=298
Score = 554 bits (1428), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 276/298 (93%), Positives = 292/298 (98%), Gaps = 0/298 (0%)
Query 1 MYNPKVMLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKT 60
MYNPKVMLIPCGQCIGCRIRQREDWTTRIELEARDYPK++VWFITLTY+DD+VPGMIVKT
Sbjct 1 MYNPKVMLIPCGQCIGCRIRQREDWTTRIELEARDYPKEQVWFITLTYNDDNVPGMIVKT 60
Query 61 GEIMRKVQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRGKLRYFVAGEYGEQTARPH 120
GEIMRKVQYTWKPG+KRP SVQILLYEDIQKFLKRLRKAY+GKLRYFVAGEYGEQTARPH
Sbjct 61 GEIMRKVQYTWKPGKKRPESVQILLYEDIQKFLKRLRKAYKGKLRYFVAGEYGEQTARPH 120
Query 121 YHMILYGWKPTDLENLYKIQHNGYYTSEWLKGLWGMGQIQIAQAVPETYRYVAGYVTKKM 180
YHMILYGW+PTDL+NLYKI HNGYYTSEWL+ LWGMGQIQIAQAVPETYRYVAGYVTKKM
Sbjct 121 YHMILYGWEPTDLKNLYKIHHNGYYTSEWLENLWGMGQIQIAQAVPETYRYVAGYVTKKM 180
Query 181 YEIDGKKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTNGKRAQIPRY 240
YE+DGKKANAYYELGQTKPFACMSLKPGLGD YYQEHKAEIWRQGYIQCTNGK+AQIPRY
Sbjct 181 YELDGKKANAYYELGQTKPFACMSLKPGLGDHYYQEHKAEIWRQGYIQCTNGKQAQIPRY 240
Query 241 YEKQMEAENPQRLWRIKQNRQKNAMQQKRLQIENQDYKTVLetkervtkkrtkkSGIL 298
YEKQMEAENPQRLWRIKQNRQKNA++QKRLQ+E QDYKT+LETKERVTKK+TKK GIL
Sbjct 241 YEKQMEAENPQRLWRIKQNRQKNAIEQKRLQLEEQDYKTILETKERVTKKQTKKRGIL 298
> Gokush_Human_gut_33_003_Microviridae_AG066_putative.VP4
Length=318
Score = 523 bits (1347), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 245/277 (88%), Positives = 259/277 (94%), Gaps = 0/277 (0%)
Query 1 MYNPKVMLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKT 60
MY VMLIPCGQCIGCRIRQREDWTTRIELEARDYP+++VWFITLTYDDDHVPGMIV T
Sbjct 21 MYRKDVMLIPCGQCIGCRIRQREDWTTRIELEARDYPREQVWFITLTYDDDHVPGMIVNT 80
Query 61 GEIMRKVQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRGKLRYFVAGEYGEQTARPH 120
GEIMRKVQYTWKPGEKRP SVQILLY DIQKFLKRLRKAYRGKLRYF+AGEYGEQTARPH
Sbjct 81 GEIMRKVQYTWKPGEKRPESVQILLYTDIQKFLKRLRKAYRGKLRYFIAGEYGEQTARPH 140
Query 121 YHMILYGWKPTDLENLYKIQHNGYYTSEWLKGLWGMGQIQIAQAVPETYRYVAGYVTKKM 180
YHMILYGW+PTDLE+LYKIQHNGY+TS+WL LWGMGQIQIAQAVPETYRYVAGYVTKKM
Sbjct 141 YHMILYGWQPTDLEHLYKIQHNGYFTSKWLANLWGMGQIQIAQAVPETYRYVAGYVTKKM 200
Query 181 YEIDGKKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTNGKRAQIPRY 240
YEIDG+KAN YYELGQTKPFACMSLKPGLGD YYQEHK EIW+QGYIQCTNGKRAQIPRY
Sbjct 201 YEIDGQKANTYYELGQTKPFACMSLKPGLGDHYYQEHKKEIWKQGYIQCTNGKRAQIPRY 260
Query 241 YEKQMEAENPQRLWRIKQNRQKNAMQQKRLQIENQDY 277
YEK MEAENPQRLWRIKQNRQ A+ + RL+ EN D+
Sbjct 261 YEKMMEAENPQRLWRIKQNRQAAAIAENRLKYENSDF 297
> Gokush_Human_feces_A_019_Microviridae_AG0436_putative.VP4
Length=338
Score = 290 bits (743), Expect = 5e-98, Method: Compositional matrix adjust.
Identities = 146/279 (52%), Positives = 189/279 (68%), Gaps = 7/279 (3%)
Query 1 MYNPKVMLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKT 60
+Y VMLIPCGQCIGCRIR+REDW RIE+EA+ VWF+TLTYDD+H+PG+ +T
Sbjct 44 IYRKDVMLIPCGQCIGCRIRKREDWACRIEMEAKTSKPHSVWFVTLTYDDEHIPGVNHET 103
Query 61 GEIMRKVQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRGKLRYFVAGEYGEQTARPH 120
GE++R Y + G+ + + Q L Y DIQKFLKRLRKA LRYF AGEYGE+T RPH
Sbjct 104 GEVIRGALYKRQKGDLKEN--QTLWYGDIQKFLKRLRKASEPGLRYFCAGEYGEKTGRPH 161
Query 121 YHMILYGWKPTDLENLYKIQHNGYYTSEWLKGLWGMGQIQIAQ-AVPETYRYVAGYVTKK 179
YH+IL+ + P LE + GYYT + W G +++ A + + Y AGYVTKK
Sbjct 162 YHLILFNYNPEKLEPYRLLSKEGYYTDTRITKCWPYGIHNMSEPATSKAFSYTAGYVTKK 221
Query 180 MYEIDGKKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTNGKRAQIPR 239
+ G + + + G PFA MSLKPGLG++YY ++K IW QGYIQ +NGKRA IPR
Sbjct 222 I----GDETMEHIKRGLRPPFAMMSLKPGLGEEYYLQNKERIWEQGYIQLSNGKRAAIPR 277
Query 240 YYEKQMEAENPQRLWRIKQNRQKNAMQQKRLQIENQDYK 278
Y+EKQMEAENP++LW IK+ RQ+ AM + ++E D K
Sbjct 278 YFEKQMEAENPEKLWEIKRQRQQKAMDSTKNKMEQTDIK 316
> Gokush_Human_feces_E_017_Microviridae_AG0124_putative.VP4
Length=338
Score = 289 bits (739), Expect = 2e-97, Method: Compositional matrix adjust.
Identities = 145/279 (52%), Positives = 188/279 (67%), Gaps = 7/279 (3%)
Query 1 MYNPKVMLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKT 60
+Y VMLIPCGQCIGCRIR+REDW RIE+EA+ VWF+TLTYDD ++PG+ +T
Sbjct 44 IYRKDVMLIPCGQCIGCRIRKREDWACRIEMEAKTSKPHSVWFVTLTYDDKNIPGIDHET 103
Query 61 GEIMRKVQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRGKLRYFVAGEYGEQTARPH 120
GE++R Y + G+ + + Q L YEDIQKFLKRLRKA +RYF AGEYGE T RPH
Sbjct 104 GEVIRGALYKRQKGDLKEN--QTLWYEDIQKFLKRLRKAAGPGIRYFCAGEYGEHTGRPH 161
Query 121 YHMILYGWKPTDLENLYKIQHNGYYTSEWLKGLWGMGQIQIAQ-AVPETYRYVAGYVTKK 179
YH+IL+ + P LE + GY+T + W G +++ A + Y Y A YVTKK
Sbjct 162 YHLILFNYNPEKLEPYRLLSKEGYFTDTRITKCWPYGIHNMSEPATSKAYNYTAAYVTKK 221
Query 180 MYEIDGKKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTNGKRAQIPR 239
+ G + + + G PFA MSLKPGLG++YY +HK EIW QGYIQ +NGKRA IPR
Sbjct 222 I----GDETMEHIKRGLRPPFAMMSLKPGLGEEYYLQHKEEIWEQGYIQLSNGKRAAIPR 277
Query 240 YYEKQMEAENPQRLWRIKQNRQKNAMQQKRLQIENQDYK 278
Y+EKQMEAE+P+RLW IK+ RQ+ +M + ++E D K
Sbjct 278 YFEKQMEAEDPERLWEIKRQRQQKSMDSTKNKMEKTDIK 316
> Gokush_Human_feces_A_020_Microviridae_AG0262_putative.VP4
Length=338
Score = 288 bits (738), Expect = 3e-97, Method: Compositional matrix adjust.
Identities = 144/279 (52%), Positives = 191/279 (68%), Gaps = 7/279 (3%)
Query 1 MYNPKVMLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKT 60
+Y VMLIPCGQCIGCRIR+REDW RIE+EA+ VWF+TLTYDD ++PG+ +T
Sbjct 44 IYRKDVMLIPCGQCIGCRIRKREDWACRIEMEAKTSKPHSVWFVTLTYDDKNIPGVNHET 103
Query 61 GEIMRKVQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRGKLRYFVAGEYGEQTARPH 120
GE++R Y + G+ + + Q L YEDIQKFLKRLRKA +RYF AGEYGE T RPH
Sbjct 104 GEVIRGALYKRQKGDLKEN--QTLWYEDIQKFLKRLRKAAGPGIRYFCAGEYGEHTGRPH 161
Query 121 YHMILYGWKPTDLENLYKIQHNGYYTSEWLKGLWGMGQIQIAQ-AVPETYRYVAGYVTKK 179
YH+IL+ + P LE + GY+T + W G +++ A + Y Y AGYVTKK
Sbjct 162 YHLILFNYNPEKLEPYRLLSKEGYFTDTRITKCWPYGIHNMSEPATSKAYSYTAGYVTKK 221
Query 180 MYEIDGKKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTNGKRAQIPR 239
+ G++ + + G PFA MSLKPGLG++YY ++K +IW+QGYIQ +NGKRA IPR
Sbjct 222 I----GEETMEHIKRGLRPPFAMMSLKPGLGEEYYLQNKEQIWKQGYIQLSNGKRAAIPR 277
Query 240 YYEKQMEAENPQRLWRIKQNRQKNAMQQKRLQIENQDYK 278
Y+EKQMEAE+P+RLW IK+ RQ+ +M + ++E D K
Sbjct 278 YFEKQMEAEDPERLWEIKRQRQQKSMDSTKSKMEQTDIK 316
> Gokush_Human_feces_B_029_Microviridae_AG0416_putative.VP4
Length=292
Score = 237 bits (604), Expect = 8e-78, Method: Compositional matrix adjust.
Identities = 128/272 (47%), Positives = 173/272 (64%), Gaps = 15/272 (6%)
Query 7 MLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKTGEIMRK 66
ML+PCG+C+GCR+RQR+DW TR+ +E++ WF+T+T++ ++VPGMI TGE++R
Sbjct 1 MLLPCGKCLGCRLRQRQDWETRMLMESKTLAP--AWFLTITWNQEYVPGMIRATGEVIRG 58
Query 67 VQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRGK------LRYFVAGEYGEQTARPH 120
+ W GE P VQILL ED+ F KRLRK + LRYF GEYGE T RPH
Sbjct 59 AVHQWTTGEV-PEVVQILLQEDMVLFNKRLRKKQQTSDKWGLDLRYFYCGEYGENTGRPH 117
Query 121 YHMILYGWKPTDLENLYKIQHNGYYTSEWLKGLWGMGQIQIAQAVPETYRYVAGYVTKKM 180
+H I YG + DL+ K N Y+ SE L +WGMG I IA+A ET YVAGYVTKK
Sbjct 118 HHGIYYGLEIPDLKK--KRGDNPYFESEELNKIWGMGNIIIAEATAETMAYVAGYVTKKA 175
Query 181 YEIDGKKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTNGKRAQIPRY 240
Y D K+ Y ELG P+ CMS PGLG YY HK ++++ + NGK+ IPRY
Sbjct 176 YGNDNKR---YRELGLPTPYCCMSRNPGLGYDYYTSHKEQMYKDDGLY-FNGKKRPIPRY 231
Query 241 YEKQMEAENPQRLWRIKQNRQKNAMQQKRLQI 272
++K+ E E+P LW IK+ RQ +A+ ++++
Sbjct 232 FDKKYEKEDPINLWSIKEKRQSSAINALKIKM 263
> Gokush_Human_feces_C_014_Microviridae_AG0151_putative.VP4
Length=331
Score = 181 bits (459), Expect = 1e-55, Method: Compositional matrix adjust.
Identities = 97/241 (40%), Positives = 142/241 (59%), Gaps = 20/241 (8%)
Query 6 VMLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKTGEIMR 65
+ L+PCG+C CR + + W TRIELEA+ + D V F+T+TYD++H+P GEI++
Sbjct 43 LALLPCGKCEYCRKQMADQWATRIELEAKRW--DNVIFVTMTYDEEHIP-----YGEILK 95
Query 66 KVQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRGKLRYFVAGEYGEQTARPHYHMIL 125
Q Q + D+Q FLKRLRKAY+ ++YF+AGEYG++T RPHYH I
Sbjct 96 GYQSI---------QSQTVSKRDVQLFLKRLRKAYKKPIKYFIAGEYGDRTKRPHYHGIF 146
Query 126 YGWKPTDLENLYKIQHNGYYTSEWLKGLWGMGQIQIAQAVPETYRYVAGYVTKKMYEIDG 185
+G KP D + N Y+ SEWL +WG G + + A P +Y YVA YV KK I
Sbjct 147 FGLKPEDGVWYKNQKGNAYFKSEWLTNIWGKGFVDFSPAAPGSYAYVAQYVNKKA--IGA 204
Query 186 KKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTNGKRAQIPRYYEKQM 245
+++ Y+ G+ F MS G+G++Y +EH EI I C G++ + PRY++K +
Sbjct 205 EQSAKYWMQGREPEFRIMS--KGIGEEYLKEHMDEILETDNITCAGGRQKRPPRYFDKLL 262
Query 246 E 246
+
Sbjct 263 D 263
> Gokush_Human_gut_32_030_Microviridae_AG0185_putative.VP4
Length=310
Score = 158 bits (399), Expect = 3e-47, Method: Compositional matrix adjust.
Identities = 100/277 (36%), Positives = 141/277 (51%), Gaps = 26/277 (9%)
Query 6 VMLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKTGEIMR 65
++ +PCGQCIGCRI W R LE + + D WF T TYDDDHVP E
Sbjct 25 LVPLPCGQCIGCRIDYSRQWANRCLLELKYH--DSAWFCTFTYDDDHVPRTYYPDPET-- 80
Query 66 KVQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRG-KLRYFVAGEYGEQTARPHYHMI 124
GE P+ L D Q +KR+RK + K+R+F++GEYG QT RPHYH I
Sbjct 81 --------GEAIPALT--LQKRDFQLLMKRIRKKFENDKIRFFMSGEYGSQTFRPHYHAI 130
Query 125 LYGWKPTDLENLYKIQHNG----YYTSEWLKGLWGMGQIQIAQAVPETYRYVAGYVTKKM 180
L+G DL+ ++ G YY S L+ W G + + + E+ Y A YV KK+
Sbjct 131 LFGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWESCAYTARYVMKKL 190
Query 181 YEIDGKKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTN---GKRAQI 237
GK+A Y + F+ MS KPG+ +Y+ E+ + Q YI + GK+ +
Sbjct 191 ---KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDENSHCVEEQ-YINVSTPKGGKKFRP 246
Query 238 PRYYEKQMEAENPQRLWRIKQNRQKNAMQQKRLQIEN 274
PRYY+K + E P++ +K R K A Q ++ N
Sbjct 247 PRYYDKLFDIECPEKSAELKSLRAKLAQQAMEAKLSN 283
> Gokush_Human_gut_31_045_Microviridae_AG0114_putative.VP4
Length=310
Score = 158 bits (399), Expect = 3e-47, Method: Compositional matrix adjust.
Identities = 100/277 (36%), Positives = 141/277 (51%), Gaps = 26/277 (9%)
Query 6 VMLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKTGEIMR 65
++ +PCGQCIGCRI W R LE + + D WF T TYDDDHVP E
Sbjct 25 LVPLPCGQCIGCRIDYSRQWANRCLLELKYH--DSAWFCTFTYDDDHVPRTYYPDPET-- 80
Query 66 KVQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRG-KLRYFVAGEYGEQTARPHYHMI 124
GE P+ L D Q +KR+RK + K+R+F++GEYG QT RPHYH I
Sbjct 81 --------GEAIPALT--LQKRDFQLLMKRIRKKFENDKIRFFMSGEYGSQTFRPHYHAI 130
Query 125 LYGWKPTDLENLYKIQHNG----YYTSEWLKGLWGMGQIQIAQAVPETYRYVAGYVTKKM 180
L+G DL+ ++ G YY S L+ W G + + + E+ Y A YV KK+
Sbjct 131 LFGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWESCAYTARYVMKKL 190
Query 181 YEIDGKKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTN---GKRAQI 237
GK+A Y + F+ MS KPG+ +Y+ E+ + Q YI + GK+ +
Sbjct 191 ---KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDENSHCVEEQ-YINVSTPKGGKKFRP 246
Query 238 PRYYEKQMEAENPQRLWRIKQNRQKNAMQQKRLQIEN 274
PRYY+K + E P++ +K R K A Q ++ N
Sbjct 247 PRYYDKLFDIECPEKSAELKSLRAKLAQQAMEAKLSN 283
> Gokush_Human_gut_27_035_Microviridae_AG0163_putative.VP4
Length=310
Score = 158 bits (399), Expect = 3e-47, Method: Compositional matrix adjust.
Identities = 100/277 (36%), Positives = 141/277 (51%), Gaps = 26/277 (9%)
Query 6 VMLIPCGQCIGCRIRQREDWTTRIELEARDYPKDEVWFITLTYDDDHVPGMIVKTGEIMR 65
++ +PCGQCIGCRI W R LE + + D WF T TYDDDHVP E
Sbjct 25 LVPLPCGQCIGCRIDYSRQWANRCLLELKYH--DSAWFCTFTYDDDHVPRTYYPDPET-- 80
Query 66 KVQYTWKPGEKRPSSVQILLYEDIQKFLKRLRKAYRG-KLRYFVAGEYGEQTARPHYHMI 124
GE P+ L D Q +KR+RK + K+R+F++GEYG QT RPHYH I
Sbjct 81 --------GEAIPALT--LQKRDFQLLMKRIRKKFENDKIRFFMSGEYGSQTFRPHYHAI 130
Query 125 LYGWKPTDLENLYKIQHNG----YYTSEWLKGLWGMGQIQIAQAVPETYRYVAGYVTKKM 180
L+G DL+ ++ G YY S L+ W G + + + E+ Y A YV KK+
Sbjct 131 LFGLHLDDLQPYKTVKEGGEYYTYYNSPSLQECWPYGYVVVGEVTWESCAYTARYVMKKL 190
Query 181 YEIDGKKANAYYELGQTKPFACMSLKPGLGDKYYQEHKAEIWRQGYIQCTN---GKRAQI 237
GK+A Y + F+ MS KPG+ +Y+ E+ + Q YI + GK+ +
Sbjct 191 ---KGKEAKFYGDHNIQPEFSLMSRKPGIARQYFDENSHCVEEQ-YINVSTPKGGKKFRP 246
Query 238 PRYYEKQMEAENPQRLWRIKQNRQKNAMQQKRLQIEN 274
PRYY+K + E P++ +K R K A Q ++ N
Sbjct 247 PRYYDKLFDIECPEKSAELKSLRAKLAQQAMEAKLSN 283
Lambda K H a alpha
0.320 0.137 0.435 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 24918430