bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Microviridae_proteins.fasta 575 sequences; 147,441 total letters Query= Contig-20_CDS_annotation_glimmer3.pl_2_2 Length=84 Score E Sequences producing significant alignments: (Bits) Value Alpavirinae_Human_gut_22_017_Microviridae_AG0397_putative.VP1 21.6 1.5 Alpavirinae_Human_gut_33_017_Microviridae_AG0154_putative.VP1 20.0 4.8 Alpavirinae_Human_feces_B_020_Microviridae_AG0354_hypothetical.... 19.2 6.2 Alpavirinae_Human_feces_B_023_Microviridae_AG0145_putative.VP1 19.6 7.4 Alpavirinae_Human_feces_B_020_Microviridae_AG0351_putative.VP1 19.2 8.9 > Alpavirinae_Human_gut_22_017_Microviridae_AG0397_putative.VP1 Length=607 Score = 21.6 bits (44), Expect = 1.5, Method: Composition-based stats. Identities = 10/24 (42%), Positives = 13/24 (54%), Gaps = 0/24 (0%) Query 3 EYVQYYSEISTKPTADYRMFKRYH 26 E +Q + EIS +DYR R H Sbjct 352 EALQRWKEISQSGDSDYREQIRKH 375 > Alpavirinae_Human_gut_33_017_Microviridae_AG0154_putative.VP1 Length=614 Score = 20.0 bits (40), Expect = 4.8, Method: Composition-based stats. Identities = 8/18 (44%), Positives = 11/18 (61%), Gaps = 0/18 (0%) Query 3 EYVQYYSEISTKPTADYR 20 E +Q + EIS +DYR Sbjct 361 EALQRWKEISQSGDSDYR 378 > Alpavirinae_Human_feces_B_020_Microviridae_AG0354_hypothetical.protein Length=80 Score = 19.2 bits (38), Expect = 6.2, Method: Compositional matrix adjust. Identities = 12/56 (21%), Positives = 27/56 (48%), Gaps = 1/56 (2%) Query 12 STKPTADYRMFKRYHAFKMKNEQEITGWKIDMNGCVLKEYLIRKKGGQIRLRLTKD 67 + K ++ FKR+ + + + G++I + K Y I+K ++ + +T D Sbjct 3 NNKRFTTFKTFKRWLWHNLHTGETLEGFQI-WEDTIFKSYNIKKTERRLLVEITYD 57 > Alpavirinae_Human_feces_B_023_Microviridae_AG0145_putative.VP1 Length=668 Score = 19.6 bits (39), Expect = 7.4, Method: Compositional matrix adjust. Identities = 6/18 (33%), Positives = 13/18 (72%), Gaps = 0/18 (0%) Query 49 KEYLIRKKGGQIRLRLTK 66 + + KKGGQ+ +++T+ Sbjct 490 RGFASNKKGGQLHIKVTE 507 > Alpavirinae_Human_feces_B_020_Microviridae_AG0351_putative.VP1 Length=651 Score = 19.2 bits (38), Expect = 8.9, Method: Compositional matrix adjust. Identities = 6/13 (46%), Positives = 12/13 (92%), Gaps = 0/13 (0%) Query 54 RKKGGQIRLRLTK 66 ++KGG+I++R T+ Sbjct 474 KQKGGKIKVRATE 486 Lambda K H a alpha 0.321 0.136 0.420 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 3625946