bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-36_CDS_annotation_glimmer3.pl_2_1
Length=264
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_gut_30_017_Microviridae_AG0205_putative.VP1 331 1e-110
Pichovirinae_JCVI_003_Microviridae_AG0344_putative.VP1 68.9 3e-15
Alpavirinae_Human_feces_A_033_Microviridae_AG0383_putative.VP1 67.4 1e-14
Alpavirinae_Human_feces_D_022_Microviridae_AG0392_putative.VP1 64.7 9e-14
Alpavirinae_Human_gut_32_012_Microviridae_AG0208_putative.VP1 63.5 2e-13
Alpavirinae_Human_gut_31_126_Microviridae_AG0306_putative.VP1 63.5 2e-13
Pichovirinae_Pavin_723_Microviridae_AG0327_putative.VP1 61.6 9e-13
Alpavirinae_Human_feces_B_023_Microviridae_AG0145_putative.VP1 55.1 2e-10
Alpavirinae_Human_feces_C_016_Microviridae_AG0274_putative.VP1 52.4 1e-09
Alpavirinae_Human_gut_30_040_Microviridae_AG0134_putative.VP1 51.2 3e-09
> Alpavirinae_Human_gut_30_017_Microviridae_AG0205_putative.VP1
Length=647
Score = 331 bits (849), Expect = 1e-110, Method: Compositional matrix adjust.
Identities = 167/268 (62%), Positives = 207/268 (77%), Gaps = 5/268 (2%)
Query 2 AGVQTIPQLAIASRLQEYKDLLGAGGSRYSDWLETFFASKIEHVDRPKLIFSASQTVNVQ 61
G++TIPQLA+A+RLQEYKDL+GA GSRYSDWL TFFASKIEHVDRPKL+FS+S VN Q
Sbjct 380 TGLKTIPQLAVATRLQEYKDLIGASGSRYSDWLYTFFASKIEHVDRPKLLFSSSVMVNSQ 439
Query 62 VIMNQSGLNNFSGTQP--LGQQGGAIAFNDRLGRRQSYYFREPGYMIDMLSIRPVYYWAT 119
V+MNQ+G + F+G + LGQ GG+IAFN LGR Q+YYF+EPGY+ DML+IRPVY+W
Sbjct 440 VVMNQAGQSGFAGGEAAALGQMGGSIAFNTVLGREQTYYFKEPGYIFDMLTIRPVYFWTG 499
Query 120 ITPDYLSYRGADYFNPIYNDIGYQDISISRIANC--PPQIDNVAV-QEPCFNEFRASYDE 176
I PDYL YRG DYFNPIYNDIGYQD+ + R+ + +V+V +EPC+NEFR+SYDE
Sbjct 500 IRPDYLEYRGPDYFNPIYNDIGYQDVPLWRLGFGWKSDAVSSVSVAKEPCYNEFRSSYDE 559
Query 177 VLGQLSSVFAAETSRPLYSYWVQQRNEslsslssGNDRSYYKFLYVDMSQVNSPFSSNSE 236
VLG L S + S PL SYWVQQR+ L LSS + L+ +++ VN+PFSS+ E
Sbjct 560 VLGSLQSTLTPKASVPLQSYWVQQRDFYLIGLSSDLNEISPSMLFTNLATVNNPFSSDME 619
Query 237 DNFFINLSYAVQKKNLVNKTFATRLSNR 264
DNFF+N+SY V KNLVNK+FATRLS+R
Sbjct 620 DNFFVNMSYKVVVKNLVNKSFATRLSSR 647
> Pichovirinae_JCVI_003_Microviridae_AG0344_putative.VP1
Length=505
Score = 68.9 bits (167), Expect = 3e-15, Method: Compositional matrix adjust.
Identities = 56/181 (31%), Positives = 82/181 (45%), Gaps = 7/181 (4%)
Query 6 TIPQLAIASRLQEYKDLLGAGGSRYSDWLETFF--ASKIEHVDRPKLIFSASQTVNVQVI 63
TI L A +LQE+ +LL GGSRY + ++TFF S + + RP+ I Q V + I
Sbjct 265 TITDLRRAFKLQEWLELLARGGSRYIEQIKTFFDVKSSDQRLQRPEYITGTKQPVIISEI 324
Query 64 MNQSGLNNFSGTQPLGQQGGAIAFNDRLGRRQSYYFREPGYMIDMLSIRP-VYYWATITP 122
+N +G +G G +A G YY E G++I +LS+ P Y I
Sbjct 325 LNTTG--ETAGLPQGNMSGHGVAVG--TGNVGKYYCEEHGFIIGILSVTPNTAYQQGIPK 380
Query 123 DYLSYRGADYFNPIYNDIGYQDISISRIANCPPQIDNVAVQEPCFNEFRASYDEVLGQLS 182
YL D++ P + IG Q I + I P D P + E++ V G+
Sbjct 381 HYLRTDKYDFYWPQFAHIGEQPIVNNEIYAYDPTGDETFGYTPRYAEYKYMPSRVAGEFR 440
Query 183 S 183
S
Sbjct 441 S 441
> Alpavirinae_Human_feces_A_033_Microviridae_AG0383_putative.VP1
Length=590
Score = 67.4 bits (163), Expect = 1e-14, Method: Compositional matrix adjust.
Identities = 59/236 (25%), Positives = 103/236 (44%), Gaps = 23/236 (10%)
Query 10 LAIASRLQEYKDLLGAGGSRYSDWLETFFASKIEHVDR-PKLIFSASQTVNVQVIMNQSG 68
+ ASR+Q Y DL AGG R SD+ E+ F K++ + P + S S +NV + +G
Sbjct 295 ITFASRMQRYMDLAFAGGGRNSDFYESQFDVKLDQDNTCPAFLGSDSFDMNVNTLYQTTG 354
Query 69 LNNFSGTQPLGQQGGAIAFNDRLGRRQSYYFREPGYMIDMLSIRP-VYYWATITPDYLSY 127
+ + PLG G ++ R RR++Y+F + GY +++ SI P VYY + I P
Sbjct 355 FED--NSSPLGAFSGQLSGGTRF-RRRNYHFNDDGYFMEITSIVPRVYYPSYINPTSRQI 411
Query 128 RGADYFNPIYNDIGYQDISISRIANCPPQIDNVAVQEPCFNEFR-------------ASY 174
+ P ++I Q + S + ++ ++ P + E R Y
Sbjct 412 SLGQQYAPALDNIAMQGLKASTVFG---EVQSLGATNPSYAENRLIIPGFKLQDFNYIGY 468
Query 175 DEVLGQLSSVFAAETSRPL--YSYWVQQRNEslsslssGNDRSYYKFLYVDMSQVN 228
+ +L + + R YWV R+ + + ++Y F+ S V+
Sbjct 469 EPAWSELMTAVSKPHGRLCNDLDYWVLSRDYGRNIAHVMDSKAYKDFIAAAGSGVD 524
> Alpavirinae_Human_feces_D_022_Microviridae_AG0392_putative.VP1
Length=589
Score = 64.7 bits (156), Expect = 9e-14, Method: Compositional matrix adjust.
Identities = 58/227 (26%), Positives = 99/227 (44%), Gaps = 21/227 (9%)
Query 10 LAIASRLQEYKDLLGAGGSRYSDWLETFFASKIEHVDR-PKLIFSASQTVNVQVIMNQSG 68
+ ASR+Q Y DL AGG R SD+ E F K+ + P + S S +NV + +G
Sbjct 294 ITFASRMQRYMDLAFAGGGRNSDFYEAQFDVKLNQDNTCPAFLGSDSFDMNVNTLYQTTG 353
Query 69 LNNFSGTQPLGQQGGAIAFNDRLGRRQSYYFREPGYMIDMLSIRP-VYYWATITPDYLSY 127
+ + PLG G ++ R RR++Y+F + GY +++ SI P VYY + I P
Sbjct 354 FED--NSSPLGAFSGQLSGGTRF-RRRNYHFNDDGYFMEITSIVPRVYYPSYINPTSRQI 410
Query 128 RGADYFNPIYNDIGYQDISISRI--------------ANCPPQIDNVAVQEPCFNEFRAS 173
+ P ++I Q + S + AN I VQ+ + + +
Sbjct 411 SLGQQYAPALDNIAMQGLKASTVFGEVQNLSAISTTYANFILSIPGFKVQDSNYVGYEPA 470
Query 174 YDEVLGQLSSVFAAETSRPLYSYWVQQRNEslsslssGNDRSYYKFL 220
+ E++ +S + YWV R+ + + ++Y F+
Sbjct 471 WSELMTAVSKPHGRLCND--LDYWVLSRDYGRNLSYVMDSKAYGDFV 515
> Alpavirinae_Human_gut_32_012_Microviridae_AG0208_putative.VP1
Length=604
Score = 63.5 bits (153), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 51/161 (32%), Positives = 78/161 (48%), Gaps = 24/161 (15%)
Query 10 LAIASRLQEYKDLLGAGGSRYSDWLET-FFASKIEHVDRPKLIFSASQTVNVQVIMNQSG 68
+A S +Q + DL AGGSRYSD++ + F S+++H P + S Q + VI +G
Sbjct 300 IAAQSHIQRWLDLALAGGSRYSDYINSQFDVSRLKHTTSPLYLGSDRQYLGSNVIYQTTG 359
Query 69 LNNFSGTQPLGQQGGAIAFNDRLGRRQSYYFREPGYMIDMLSIRPVYYWATITPDYLSYR 128
+ PLG G + + RR+SY+F E GY + M A++ PD + R
Sbjct 360 AGD--SASPLGAFAGQSSGGETF-RRRSYHFGENGYFVVM---------ASLVPDVVYSR 407
Query 129 GADYFNPIYNDIGYQDISISRIANCPPQIDNVAVQEPCFNE 169
G D FN + D+ + P +DN+A+ EP E
Sbjct 408 GMDPFN---REKTLGDVYV-------PALDNIAM-EPLMIE 437
> Alpavirinae_Human_gut_31_126_Microviridae_AG0306_putative.VP1
Length=604
Score = 63.5 bits (153), Expect = 2e-13, Method: Compositional matrix adjust.
Identities = 51/161 (32%), Positives = 78/161 (48%), Gaps = 24/161 (15%)
Query 10 LAIASRLQEYKDLLGAGGSRYSDWLET-FFASKIEHVDRPKLIFSASQTVNVQVIMNQSG 68
+A S +Q + DL AGGSRYSD++ + F S+++H P + S Q + VI +G
Sbjct 300 IAAQSHIQRWLDLALAGGSRYSDYINSQFDVSRLKHTTSPLYLGSDRQYLGSNVIYQTTG 359
Query 69 LNNFSGTQPLGQQGGAIAFNDRLGRRQSYYFREPGYMIDMLSIRPVYYWATITPDYLSYR 128
+ PLG G + + RR+SY+F E GY + M A++ PD + R
Sbjct 360 AGD--SASPLGAFAGQSSGGETF-RRRSYHFGENGYFVVM---------ASLVPDVVYSR 407
Query 129 GADYFNPIYNDIGYQDISISRIANCPPQIDNVAVQEPCFNE 169
G D FN + D+ + P +DN+A+ EP E
Sbjct 408 GMDPFN---REKTLGDVYV-------PALDNIAM-EPLMIE 437
> Pichovirinae_Pavin_723_Microviridae_AG0327_putative.VP1
Length=508
Score = 61.6 bits (148), Expect = 9e-13, Method: Compositional matrix adjust.
Identities = 54/197 (27%), Positives = 87/197 (44%), Gaps = 16/197 (8%)
Query 6 TIPQLAIASRLQEYKDLLGAGGSRYSDWLETFFA--SKIEHVDRPKLIFSASQTVNVQVI 63
TI L A +LQE+ + GG+RY + + T F S + + RP+ I V V +
Sbjct 268 TINDLRRAFKLQEWLEKNARGGTRYIENILTHFGVRSSDKRLQRPEYITGVKSPVVVSEV 327
Query 64 MNQSGLNNFSGTQPLGQQGGAIAFNDRLGRRQSYYFREPGYMIDMLSIRP-VYYWATITP 122
+N +G + G P G G + G+ SYY E GY+I ++S+ P Y I
Sbjct 328 LNTTGQD---GGLPQGNMAGH-GISVTSGKSGSYYCEEHGYIIGIMSVMPKTAYQQGIPR 383
Query 123 DYLSYRGADYFNPIYNDIGYQDISISRIANCPPQIDNVAVQEPCFNEFRASYDEVLGQ-- 180
+L DYF P + +IG Q+++ + ++ P + E++ V G+
Sbjct 384 TFLKTDSLDYFWPTFANIGEQEVAKQELYAYTANANDTFGYVPRYAEYKYMPSRVAGEFR 443
Query 181 -------LSSVFAAETS 190
L +FA E S
Sbjct 444 TSLNYWHLGRIFATEPS 460
> Alpavirinae_Human_feces_B_023_Microviridae_AG0145_putative.VP1
Length=668
Score = 55.1 bits (131), Expect = 2e-10, Method: Compositional matrix adjust.
Identities = 60/261 (23%), Positives = 115/261 (44%), Gaps = 28/261 (11%)
Query 6 TIPQLAIASRLQEYKDLLGAGGSRYSDWLETFFASKIE-HVDRPKLIFSASQTVNVQVIM 64
TI QL ++ ++ + + + G Y DW+ET + S+ H + P S + Q ++
Sbjct 415 TIDQLNLSKKVYDMLNRIAISGGTYQDWVETVYTSEWNMHTETPVYEGGMSAEIEFQEVV 474
Query 65 NQSGLNNFSGTQPLGQQGGAIAFNDRLGRRQSYYFREPGYMIDMLSIRP-VYY-----WA 118
+ S +PLG G +++ G + EP Y++ + SI P V Y W
Sbjct 475 SNSATEE----EPLGTLAGRGFASNKKGGQLHIKVTEPCYIMGIASITPRVDYCQGNDWD 530
Query 119 TITPDYLSYRGADYFNPIYNDIGYQDISISRIANCPPQIDNVAV-QEPCFNEFRASYDEV 177
+ D + D P + IGYQD+ ++ + N+AV ++P + + S+++
Sbjct 531 ITSLDTMD----DIHKPQLDSIGYQDLMQEQMNAQASR--NLAVGKQPSWINYMTSFNKT 584
Query 178 LGQLSSVFAAETSRPLYSYWVQQRNEslsslssGND---RSYYKFLYVDMSQVNSPFSSN 234
G FA E ++ V R + + +G + + Y Y+D SQ N F+
Sbjct 585 YG----TFANEDGEG-EAFMVLNRYFDIKEIDAGTETGVKVYNTSTYIDPSQYNYIFAET 639
Query 235 SED--NFFINLSYAVQKKNLV 253
NF++ L + ++ + ++
Sbjct 640 GTKSMNFWVQLGFGIEARRVM 660
> Alpavirinae_Human_feces_C_016_Microviridae_AG0274_putative.VP1
Length=641
Score = 52.4 bits (124), Expect = 1e-09, Method: Compositional matrix adjust.
Identities = 47/160 (29%), Positives = 72/160 (45%), Gaps = 19/160 (12%)
Query 7 IPQLAIASRLQEYKDLLGAGGSRYSDWLETFFASK--IEHVDRPKLIFSASQTVN---VQ 61
+P+L + +++Q + D L G R D T + +K +V++P + ++N V+
Sbjct 357 VPELRLKTKIQNWMDRLFISGGRVGDVFRTLWGTKSSAPYVNKPDFLGVWQASINPSNVR 416
Query 62 VIMNQSGLNNFSGTQP-LGQQGGAIA----FNDRLGRRQSYYFREPG--YMIDMLSIRPV 114
+ N S SG LGQ + F+D G YY +EPG +I ML P
Sbjct 417 AMANGSA----SGEDANLGQLAACVDRYCDFSDHSG--IDYYAKEPGTFMLITMLVPEPA 470
Query 115 YYWATITPDYLSYRGADYFNPIYNDIGYQDISISRIANCP 154
Y + PD S D FNP N IG+Q + R + P
Sbjct 471 YSQG-LHPDLASISFGDDFNPELNGIGFQLVPRHRFSMMP 509
> Alpavirinae_Human_gut_30_040_Microviridae_AG0134_putative.VP1
Length=679
Score = 51.2 bits (121), Expect = 3e-09, Method: Compositional matrix adjust.
Identities = 42/187 (22%), Positives = 85/187 (45%), Gaps = 8/187 (4%)
Query 2 AGVQTIPQLAIASRLQEYKDLLGAGGSRYSDWLETFFASK-IEHVDRPKLIFSASQTVNV 60
+G T+ L +A ++ + + Y+ W++T + S + H++ P + +S +
Sbjct 434 SGSFTLDTLNLAKKVYTMLNRIAVSDGSYNAWIQTVYTSGGLNHIETPLYLGGSSLEIEF 493
Query 61 QVIMNQSGLNNFSGTQPLGQQGGAIAFNDRLGRRQSYYFREPGYMIDMLSIRP-VYYWAT 119
Q ++N SG + QPLG G + G + EPGY+ + SI P V Y+
Sbjct 494 QEVINNSGTED----QPLGALAGRGVATNHKGGNIVFKADEPGYIFCITSITPRVDYFQG 549
Query 120 ITPDYLSYRGADYFNPIYNDIGYQDISISRI-ANCPPQIDNVAV-QEPCFNEFRASYDEV 177
D D P + IG+QD + ++C + N+++ ++P + ++ + ++
Sbjct 550 NEWDLYLESLDDLHKPQLDGIGFQDRLYRHLNSSCVREDLNISIGKQPAWVQYMTNVNKT 609
Query 178 LGQLSSV 184
G + V
Sbjct 610 YGNFALV 616
Lambda K H a alpha
0.319 0.135 0.400 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 21456452