bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-24_CDS_annotation_glimmer3.pl_2_2
Length=647
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_D_008_Microviridae_AG098_putative.VP1 700 0.0
Alpavirinae_Human_feces_D_031_Microviridae_AG0425_putative.VP1 697 0.0
Alpavirinae_Human_feces_A_021_Microviridae_AG077_putative.VP1 503 9e-172
Alpavirinae_Human_feces_C_043_Microviridae_AG0322_putative.VP1 494 1e-168
Alpavirinae_Human_gut_33_017_Microviridae_AG0154_putative.VP1 432 2e-144
Alpavirinae_Human_gut_22_017_Microviridae_AG0397_putative.VP1 429 2e-143
Alpavirinae_Human_feces_E_011_Microviridae_AG0388_putative.VP1 421 6e-140
Alpavirinae_Human_feces_D_015_Microviridae_AG019_putative.VP1 407 6e-135
Alpavirinae_Human_feces_A_034_Microviridae_AG0105_putative.VP1 394 8e-130
Alpavirinae_Human_gut_33_005_Microviridae_AG0184_putative.VP1 382 5e-125
> Alpavirinae_Human_feces_D_008_Microviridae_AG098_putative.VP1
Length=618
Score = 700 bits (1807), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 386/654 (59%), Positives = 465/654 (71%), Gaps = 47/654 (7%)
Query 4 NLFSFGDVRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPVN 63
+LF+ V+NHP RSGFDLS R+CFTSKAGELLPV++ +VYPGD F+I+ QLFTRTQP+N
Sbjct 2 SLFNMSAVKNHPRRSGFDLSNRVCFTSKAGELLPVFWDIVYPGDSFKIKTQLFTRTQPLN 61
Query 64 TAAYTRIREYLDWYFVPLRLINKNLPQALMNMQDNPVQASGIVSNKIVTSDIPWTLLGHD 123
TAAYTRIREYLD+YFVPLRLINKNLP AL MQDNPVQA+G+ SNK+VT+DIPW + H
Sbjct 62 TAAYTRIREYLDFYFVPLRLINKNLPTALTQMQDNPVQATGLSSNKVVTTDIPWVPV-HS 120
Query 124 SKPLGIANLLFRMYKGD-SSAQIDPVLNFFGFNSGTLGAKLAMMLRYGNFISKDYSASTP 182
S + F +G SS+ I+ NF GF+S T AKL M LRYGNF+S S
Sbjct 121 SSGSYSSLTGFADVRGSVSSSDIE---NFLGFDSITQSAKLLMYLRYGNFLS---SVVPD 174
Query 183 GDLSFGLSKSDFDLR---AYNSSY-AVNILPFAVYQKIYADHFRFSQWEKNEPYTYNFDW 238
S GLS S DLR ++ Y +V+ILP A YQKIYAD FRF+QWEKN+PYTYNFDW
Sbjct 175 EQKSIGLSSS-LDLRNSETVSTGYTSVHILPLATYQKIYADFFRFTQWEKNQPYTYNFDW 233
Query 239 YSGGNVFNVFALGAADSTLKTYLEGNNLFTLRYANWPKDLFMGVMPNSQLGDVSVVDITT 298
YSGGNV A + K Y +NLFTLRYANWPKD+FMGVMP+SQLGDVS+VD T
Sbjct 234 YSGGNVL---ASLTTQALAKKYYSDDNLFTLRYANWPKDMFMGVMPDSQLGDVSIVD--T 288
Query 299 DSATRSYPVSLFSSASGPN--GINKLGAAIPDAPFNEATDTPSTYNFSIDFGTEKWDSSI 356
+ ++PV L++ A G + G+ + P A + T S + S +G +
Sbjct 289 SGSEGTFPVGLYNFADGGSRAGLVAVSGTSPAAGSSLDMQTTSALSASTKYGVYAQQVA- 347
Query 357 KAKTWvgvnnvgsgvlgvtvPGSQLAASFSVLQLRMAEAVQKYREVSQVADQTVRDQIYA 416
L +SFS+LQLRMAEAVQKYREVSQ ADQ R QI A
Sbjct 348 -----------------------GLGSSFSILQLRMAEAVQKYREVSQFADQDARGQIMA 384
Query 417 HFGVSLSPALSDTCFRVGGSASNIDISEVVNNNLAGENQADIMgkgvgtgqggTSFSSDE 476
HFGVSLSP LSD C +GGS+SNID+SEVVN N+ G+N A+I GKGVGTGQG S + D
Sbjct 385 HFGVSLSPVLSDKCMYLGGSSSNIDLSEVVNTNITGDNIAEIAGKGVGTGQGSFSGNFDT 444
Query 477 YGVLMAIYHVVPLLDYVITGQPHELLYTNTSDLPFPEFDSIGMQSLHFGRFFNYKSDKFT 536
YG++M IYH VPLLDYVITGQP LLYTNT+DLPFPE+DSIGMQ++ FGRF N K+ +T
Sbjct 445 YGIIMGIYHNVPLLDYVITGQPQNLLYTNTADLPFPEYDSIGMQTIQFGRFVNSKAVGWT 504
Query 537 --FDPTASVMGYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLDPEYLSKWIDSTV-SAGQT 593
D MGY+PRF D+KT YDEV GAFRSTLK+WVAPLDP L +W+ ++V S+G+
Sbjct 505 SGVDYRTQTMGYLPRFFDVKTRYDEVLGAFRSTLKNWVAPLDPANLPQWLQTSVTSSGKL 564
Query 594 YYSLNYGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAVRNFDYDGMPY 647
+ +LNYGFFKVNP VLDSIF VK DS++DTDQFL++LY+D+KAVRNFDYDGMPY
Sbjct 565 FLNLNYGFFKVNPRVLDSIFNVKCDSTIDTDQFLTTLYMDIKAVRNFDYDGMPY 618
> Alpavirinae_Human_feces_D_031_Microviridae_AG0425_putative.VP1
Length=614
Score = 697 bits (1800), Expect = 0.0, Method: Compositional matrix adjust.
Identities = 382/652 (59%), Positives = 456/652 (70%), Gaps = 56/652 (9%)
Query 11 VRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPVNTAAYTRI 70
V+NHP RSGFDLS R+CFTSKAGELLPV++ +VYPGD F+I+ QLFTRTQP+NTAAYTRI
Sbjct 4 VKNHPRRSGFDLSNRVCFTSKAGELLPVFWDIVYPGDSFKIKTQLFTRTQPLNTAAYTRI 63
Query 71 REYLDWYFVPLRLINKNLPQALMNMQDNPVQASGIVSNKIVTSDIPWT---LLGHDSKPL 127
REYLD+YFVPLRLINKNLP ALM MQDNPVQA+G+ SNK+VT+DIPW L G
Sbjct 64 REYLDFYFVPLRLINKNLPTALMQMQDNPVQATGLSSNKVVTTDIPWVPTNLSGTYGSLT 123
Query 128 GIANLLFRMYKGDSSAQIDPVLNFFGFNSGTLGAKLAMMLRYGNFISKDYSASTPGDLSF 187
+A++ K + + + GF++ T AKL M LRYGNF+S S + + S
Sbjct 124 ALADV-----KNSFPSSSTGIEDLLGFDAITQSAKLLMYLRYGNFLS---SVVSDKNKSL 175
Query 188 GLSKSDFDLR----AYNSSYAVNILPFAVYQKIYADHFRFSQWEKNEPYTYNFDWYSGGN 243
GLS S DLR A +++ILP A YQK YAD FRF+QWEKN+PYTYNFDWYSGGN
Sbjct 176 GLSGS-LDLRNSETASTGYTSMHILPLAAYQKAYADFFRFTQWEKNQPYTYNFDWYSGGN 234
Query 244 VFNVFALGAADSTL---KTYLEGNNLFTLRYANWPKDLFMGVMPNSQLGDVSVVDITTDS 300
V A+ +TL K Y +NLFTLRYANWPKD+FMGVMP+SQLGDVS+VD +
Sbjct 235 VL------ASLTTLDLAKKYYSDDNLFTLRYANWPKDMFMGVMPDSQLGDVSIVDASGSE 288
Query 301 ATRSYPVSLFSSASGP--NGINKLGAAIPDAPFNEATDTPSTYNFSIDFGTEKWDSSIKA 358
T +PV L G G+ + P + T S + + +G
Sbjct 289 GT--FPVGLLDVNDGTLRAGLLARSGSAPAEKSSLEMQTSSALSANTTYGV--------- 337
Query 359 KTWvgvnnvgsgvlgvtvPGSQLAASFSVLQLRMAEAVQKYREVSQVADQTVRDQIYAHF 418
+ LA+SFS+LQLRMAEAVQKYREVSQ ADQ R QI AHF
Sbjct 338 ---------------YAQRAAGLASSFSILQLRMAEAVQKYREVSQFADQDARGQIMAHF 382
Query 419 GVSLSPALSDTCFRVGGSASNIDISEVVNNNLAGENQADIMgkgvgtgqggTSFSSDEYG 478
GVSLSP LSD C +GGS+SNID+SEVVN N+ G+N A+I GKGVGTGQG S DEYG
Sbjct 383 GVSLSPVLSDKCVYLGGSSSNIDLSEVVNTNITGDNVAEIAGKGVGTGQGSFSGQFDEYG 442
Query 479 VLMAIYHVVPLLDYVITGQPHELLYTNTSDLPFPEFDSIGMQSLHFGRFFNYKSDKFT-- 536
+++ IYH VPLLDYVITGQP LLYTNT+DLPFPEFDSIGMQ++ FGRF N KS +T
Sbjct 443 IIIGIYHNVPLLDYVITGQPQNLLYTNTADLPFPEFDSIGMQTIQFGRFVNSKSVSWTSG 502
Query 537 FDPTASVMGYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLDPEYLSKWIDSTV-SAGQTYY 595
D MGY+PRF D+KT YDEV GAFRSTLK+WVAPLDP Y+SKW+ S+V S+G+
Sbjct 503 VDYRVQTMGYLPRFFDVKTRYDEVLGAFRSTLKNWVAPLDPSYVSKWLQSSVTSSGKLAL 562
Query 596 SLNYGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAVRNFDYDGMPY 647
+LNYGFFKVNP VLDSIF VK DS++DTDQFL++LY+D+KAVRNFDYDGMPY
Sbjct 563 NLNYGFFKVNPRVLDSIFNVKCDSTIDTDQFLTALYMDIKAVRNFDYDGMPY 614
> Alpavirinae_Human_feces_A_021_Microviridae_AG077_putative.VP1
Length=622
Score = 503 bits (1294), Expect = 9e-172, Method: Compositional matrix adjust.
Identities = 289/657 (44%), Positives = 394/657 (60%), Gaps = 51/657 (8%)
Query 5 LFSFGDVRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPVNT 64
LFS+ D++NHP RSGFDL++RICFTSKAGELLPVY+K PGDK+ I FTRTQPV+T
Sbjct 3 LFSYDDLKNHPRRSGFDLTKRICFTSKAGELLPVYWKPTIPGDKWNISTNWFTRTQPVDT 62
Query 65 AAYTRIREYLDWYFVPLRLINKNLPQALMNMQDNPVQASGIVSNKIVTSDIPWTLLGHDS 124
AAYTR++EY+DW+FVPL LI K + A+ M DNPV A + N+ +T+D+P+T L S
Sbjct 63 AAYTRVKEYVDWFFVPLNLIQKGIESAITQMVDNPVSAMSAIENRAITTDMPYTTLLSLS 122
Query 125 KPLGIANLLFRMYKGDSSAQIDPVLNFFGFNSGTLGAKLAMMLRYGNFISKDYSA-STPG 183
+ L + N + Y + + LN FGF+ L AKL ML+YGNFI+ ++S TP
Sbjct 123 RALYMLN--GKSYVNSHAGK----LNMFGFSRADLSAKLLQMLKYGNFINPEHSGLDTP- 175
Query 184 DLSFGLSKSDFDLRAYNSSYAVNILPFAVYQKIYADHFRFSQWEKNEPYTYNFDWYSGGN 243
FG S +Y + AVN+LP YQKIY+D+FRF QWEK PYTYN D+Y+GG+
Sbjct 176 --MFGYSTVKLAQFSYLWNQAVNVLPIFCYQKIYSDYFRFQQWEKPVPYTYNADYYAGGD 233
Query 244 VFNVFALGAADSTLKTYLEGNNLFTLRYANWPKDLFMGVMPNSQLGDVSVVDITTDS-AT 302
+F + Y F LRY NW +DL+ G +PN Q G+VSVVD+T S
Sbjct 234 IF-----PNPTTMPDAYWNNYTPFDLRYCNWNRDLYTGFLPNQQFGNVSVVDMTVSSDEI 288
Query 303 RSYPVSLFSSASGPNGINKLGAAIPDAPFNEATDTPSTYNFSIDFGTEKWDSSIKAKTWv 362
+ PV+ A+G N + AA+ + + T ST + GT +S+ A+
Sbjct 289 MAAPVTF--GATG-NRVAVTKAALASSTSSTGIGTVSTPS----GGTVPVGTSLYAQV-- 339
Query 363 gvnnvgsgvlgvtvPGSQLAASFSVLQLRMAEAVQKYREVSQVADQTVRDQIYAHFGVSL 422
L +FS+L LR AE +QK++E++ DQ R QI HFGV L
Sbjct 340 --------------QQRDLTGAFSILALRQAEFLQKWKEIALSGDQDYRSQIEKHFGVKL 385
Query 423 SPALSDTCFRVGGSASNIDISEVVNNNLAGENQAD-------IMgkgvgtgqggTSFSSD 475
LS +GG + +DISEVVN NL + +D I GKGV +G G ++++
Sbjct 386 PAELSYMSQYIGGQFAQMDISEVVNQNLTDQAGSDAAQYPALIAGKGVNSGDGNVNYTAR 445
Query 476 EYGVLMAIYHVVPLLDYVITGQPHELLYTNTSDLPFPEFDSIGMQSLHFGRFFNYKS--- 532
++G++M IYH VPLLDY TGQ +LL T+ + PEFD+IGMQ+L G FN
Sbjct 446 QHGIIMGIYHAVPLLDYERTGQDQDLLITSAEEWAIPEFDAIGMQTLPLGTLFNSNKVSG 505
Query 533 -DKFTFDPTASVMGYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLDPEYLSKWIDSTVSAG 591
F A +GYVPR+++ KTD DE++GAFRS+ K+WVAP+D ++++ W+ +
Sbjct 506 DSDFRLHGAAYPIGYVPRYVNWKTDIDEIFGAFRSSEKTWVAPIDADFITNWVKNVADNA 565
Query 592 QTYYSL-NYGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAVRNFDYDGMPY 647
SL NY +FKVNP++LD IF VKADS+MDTD F + + + +K VRN DY GMPY
Sbjct 566 SAVQSLFNYNWFKVNPAILDDIFAVKADSTMDTDTFKTRMMMSIKCVRNLDYSGMPY 622
> Alpavirinae_Human_feces_C_043_Microviridae_AG0322_putative.VP1
Length=602
Score = 494 bits (1272), Expect = 1e-168, Method: Compositional matrix adjust.
Identities = 293/649 (45%), Positives = 387/649 (60%), Gaps = 52/649 (8%)
Query 3 SNLFSFGDVRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPV 62
+NLFS+G V+N P RSGFD S FT+KAGELLPVY+K++ PG K + FTRT PV
Sbjct 2 ANLFSYGSVKNKPARSGFDWSEHFSFTAKAGELLPVYWKMLLPGTKVNLNLSSFTRTMPV 61
Query 63 NTAAYTRIREYLDWYFVPLRLINKNLPQALMNMQDNPVQASGIVSNKIVTSDIPWTLLGH 122
NTAAYTR++EY DWYFVPLRLINK++ QAL+ MQD PVQA+ IV+NK VT D+PWT
Sbjct 62 NTAAYTRVKEYYDWYFVPLRLINKSIGQALVQMQDQPVQATSIVANKSVTLDLPWTNAAT 121
Query 123 DSKPLGIANLLFRMYKGDSSAQIDPVLNFFGFNSGTLGAKLAMMLRYGNFISKDYSASTP 182
L AN++ + N GF+ AKL LRYGN +
Sbjct 122 MFTLLNYANVI-----------LTNKYNLDGFSKAATSAKLLRYLRYGNCYYTSDPSKVG 170
Query 183 GDLSFGL-SKSDFDLRAYNSSYAVNILPFAVYQKIYADHFRFSQWEKNEPYTYNFDWYSG 241
+ +FGL SK DF+L A + + N+LP A YQKIY D FRF QWE PYTYNFD+Y+G
Sbjct 171 KNKNFGLSSKDDFNLLAA-KNVSFNVLPLAAYQKIYCDWFRFEQWENACPYTYNFDYYNG 229
Query 242 GNVFNVFALGAADSTLKTYLEGNNLFTLRYANWPKDLFMGVMPNSQLGDVSVVDITT-DS 300
GNVF + + + +N+ +LRYAN+ KDLFMGVMP+SQ G V+ V++ S
Sbjct 230 GNVF-----AGVTANPENFWSNDNILSLRYANYNKDLFMGVMPSSQFGSVATVNVANFSS 284
Query 301 ATRSYPVSLFSSASGPNGINKLGAAIPDAPFNEATDTPSTYNFSIDFGTEKWDSSIKAKT 360
+ S P+ S+ N + P + DT + F I S+I
Sbjct 285 SNLSSPLRNLSNVGMVTASNNASSGSP-LILRSSQDTSAGDGFGI------LTSNIF--- 334
Query 361 WvgvnnvgsgvlgvtvPGSQLAASFSVLQLRMAEAVQKYREVSQVADQTVRDQIYAHFGV 420
S L+ASF +L R+AEA QK++EV+Q A Q ++Q+ AHF V
Sbjct 335 ------------------STLSASFDILSFRIAEATQKWKEVTQCAKQGYKEQLEAHFNV 376
Query 421 SLSPALSDTCFRVGGSASNIDISEVVNNNLAGENQADIMgkgvgtgqggTSFSSDEYGVL 480
LS ALSD C +GG++S + ISEV+N NL ADI GKGVG G +F ++E+G+L
Sbjct 377 KLSEALSDHCRYIGGTSSGVTISEVLNTNLESA-AADIKGKGVGGSFGSETFETNEHGIL 435
Query 481 MAIYHVVPLLDYVITGQPHELLYTNTSDLPFPEFDSIGMQSLHFGRFFNYKSDKFT-FDP 539
M IYH P+LDY+ +GQ +LL T +D+P PEFD IGM++L FN +S + T
Sbjct 436 MCIYHATPVLDYLRSGQDLQLLSTLATDIPIPEFDHIGMEALPIETLFNEQSTEATALIN 495
Query 540 TASVMGYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLD-PEYLSKWIDSTVSAGQTYYSLN 598
+ V+GY PR+I KT D V GAF +TL SWVAPL E ++K + + S + YS+N
Sbjct 496 SIPVLGYSPRYIAYKTSVDWVSGAFETTLDSWVAPLTVNEQITKLLFNPDSG--SVYSMN 553
Query 599 YGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAVRNFDYDGMPY 647
YGFFKV P VLD IF + + D+DQFL ++ +VK V+N DY+GMPY
Sbjct 554 YGFFKVTPRVLDPIFVQECTDTWDSDQFLVNVSFNVKPVQNLDYNGMPY 602
> Alpavirinae_Human_gut_33_017_Microviridae_AG0154_putative.VP1
Length=614
Score = 432 bits (1111), Expect = 2e-144, Method: Compositional matrix adjust.
Identities = 269/657 (41%), Positives = 363/657 (55%), Gaps = 66/657 (10%)
Query 8 FGDVRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPVNTAAY 67
+++NHPHRSGFD+ R+ FT+K GELLPVY+ + PGDK++ + FTRTQPV T+AY
Sbjct 7 LSNLQNHPHRSGFDIGRKNAFTAKVGELLPVYWDISMPGDKYRFNVEYFTRTQPVATSAY 66
Query 68 TRIREYLDWYFVPLRLINKNLPQALMNMQD-NPVQASGIVSNKIVTSDIPWTLLGHDSKP 126
TR+REY D+Y VPLRL+ K+ P L MQD N +QA + N + + +P S
Sbjct 67 TRLREYFDFYAVPLRLLWKSAPSVLTQMQDVNQIQALSLTQNLSLGTYLP-------SLT 119
Query 127 LGIANLLFRMYKGDS--SAQIDPVLNFFGFNSGTLGAKLAMMLRYGNFISKDYSASTPGD 184
+G R G++ + N FGF+ L KL L YGN I TP
Sbjct 120 IGTLGWAIRYLNGNTWEPETASYLRNAFGFSRADLSFKLLSYLGYGNLIE------TPPS 173
Query 185 L-----SFGLSKSDFDLRAYNSSYA----VNILPFAVYQKIYADHFRFSQWEKNEPYTYN 235
L S L +D D Y Y VNI P YQKIY D FR+ QWEK+ P +YN
Sbjct 174 LGNRWWSTSLKNTD-DGANYTQQYIQNTIVNIFPLLTYQKIYQDFFRWPQWEKSNPSSYN 232
Query 236 FDWYSGGNVFNVFALGAADSTLKTYLEGNNLFTLRYANWPKDLFMGVMPNSQLGDVSVVD 295
D++SG + V L AA S Y + + +F L+Y NW KD+ MGV+PNSQ GDV+V+D
Sbjct 233 VDYFSGSSPSIVSDLPAASSA---YWKSDTMFDLKYCNWNKDMLMGVLPNSQFGDVAVLD 289
Query 296 ITTDS-ATRSYPVSLFSSASG-PNGINKLGAAIPDAPFNEATDTPSTYNFSIDFGTEKWD 353
I++ + V S G + I A +P + +T P + +
Sbjct 290 ISSSGDSDVVLGVDPHKSTLGIGSAITSKSAVVPLFALDASTSNPVSVGSKLHLDL---- 345
Query 354 SSIKAKTWvgvnnvgsgvlgvtvPGSQLAASFSVLQLRMAEAVQKYREVSQVADQTVRDQ 413
SSIK++ F+VL LR AEA+Q+++E+SQ D R+Q
Sbjct 346 SSIKSQ-------------------------FNVLALRQAEALQRWKEISQSGDSDYREQ 380
Query 414 IYAHFGVSLSPALSDTCFRVGGSASNIDISEVVNNNLAGENQADIMgkgvgtgqggT-SF 472
I HFGV L ALS+ C +GG + N+DISEVVNNNLA E ++ G+ ++
Sbjct 381 ILKHFGVKLPQALSNLCTYIGGISRNLDISEVVNNNLAAEEDTAVIAGKGVGTGNGSFTY 440
Query 473 SSDEYGVLMAIYHVVPLLDYVITGQPHELLYTNTSDLPFPEFDSIGMQSLHFGRFFNYKS 532
+++E+ V+M IYH VPLLDY +TGQ +LL T+ LP PEFD+IG++ L + FN S
Sbjct 441 TTNEHCVIMGIYHAVPLLDYTLTGQDGQLLVTDAESLPIPEFDNIGLEVLPMAQIFN-SS 499
Query 533 DKFTFDPTASVMGYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLDPEYLSKW--IDSTVSA 590
F+ + GY PR+ + KT D + GAF +TLKSWV+P+ LS W ++ S
Sbjct 500 LATAFNLFNA--GYNPRYFNWKTKLDVINGAFTTTLKSWVSPVSESLLSGWARFGASDSK 557
Query 591 GQTYYSLNYGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAVRNFDYDGMPY 647
T LNY FFKVNPSVLD IF VKADS+ DTDQ L + Y+ VRN DG+PY
Sbjct 558 TGTKAVLNYKFFKVNPSVLDPIFGVKADSTWDTDQLLVNSYIGCYVVRNLSRDGVPY 614
> Alpavirinae_Human_gut_22_017_Microviridae_AG0397_putative.VP1
Length=607
Score = 429 bits (1104), Expect = 2e-143, Method: Compositional matrix adjust.
Identities = 264/655 (40%), Positives = 360/655 (55%), Gaps = 67/655 (10%)
Query 10 DVRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPVNTAAYTR 69
+++NHPHRSGFD+ R+ FT+K GELLPVY+ + PGDK++ + FTRTQPV T+AYTR
Sbjct 3 NLQNHPHRSGFDIGRKNAFTAKVGELLPVYWDISMPGDKYKFNVEYFTRTQPVETSAYTR 62
Query 70 IREYLDWYFVPLRLINKNLPQALMNMQD-NPVQASGIVSNKIVTSDIPWTLLGHDSKPLG 128
+REY D+Y VPLRL+ K+ P L MQD N +QA N + S P L + L
Sbjct 63 LREYFDFYAVPLRLLWKSAPSVLTQMQDINQLQALSFTQNLSLGSYFPSLTLSRFTAVLN 122
Query 129 IANLLFRMYKGDSSAQIDPVLNFFGFNSGTLGAKLAMMLRYGNFISKDYSASTPGDLSFG 188
N + G+SS LN FGF+ L KL L YGN S ++S+S S
Sbjct 123 RLNGGSNV-PGNSST----FLNEFGFSRADLAFKLFSYLGYGNVWSSEFSSSNRW-WSTS 176
Query 189 LSKSDFDLRAYNSSYAVNILPFAVYQKIYADHFRFSQWEKNEPYTYNFDWYSGGNVFNVF 248
L + Y VN+ P YQKIY D FR+SQWE + P +YN D+++G + V
Sbjct 177 LKGGGSYTQQYVQDSYVNLFPLLAYQKIYQDFFRWSQWENSNPSSYNVDYFTGVSPLLVS 236
Query 249 ALGAADSTLKTYLEGNNLFTLRYANWPKDLFMGVMPNSQLGDVSVVDITTDSATRSYPVS 308
+L A + Y + +F L+Y NW KD+ MGV+PNSQ GDV+V+DI D++ + V
Sbjct 237 SLPEASNA---YWKSPTMFDLKYCNWNKDMLMGVLPNSQFGDVAVLDI--DNSGKPDVVL 291
Query 309 LFSSASGPNGINK-LGAAIPDAPF-----NEATDTPSTYNFSIDFGTEKWDSSIKAKTWv 362
+A+ G+ + + PF + A P +D +
Sbjct 292 GLGNANSTVGVASYVSSNTASIPFFALKASSANTLPVGSTLRVDLAS------------- 338
Query 363 gvnnvgsgvlgvtvPGSQLAASFSVLQLRMAEAVQKYREVSQVADQTVRDQIYAHFGVSL 422
L + F+VL LR AEA+Q+++E+SQ D R+QI HFGV+L
Sbjct 339 ------------------LKSQFTVLALRQAEALQRWKEISQSGDSDYREQIRKHFGVNL 380
Query 423 SPALSDTCFRVGGSASNIDISEVVNNNLAGENQADIMgkgvgtgqggT-SFSSDEYGVLM 481
ALS+ C +GG + N+DISEVVNNNLA E ++ G+ +++++E+ V+M
Sbjct 381 PQALSNMCTYIGGISRNLDISEVVNNNLAAEGDTAVIAGKGVGAGNGSFTYTTNEHCVVM 440
Query 482 AIYHVVPLLDYVITGQPHELLYTNTSDLPFPEFDSIGMQSLHFGRFFNYKSDKFTFDPTA 541
IYH VPLLDY ITGQ +LL T+ LP PEFD+IGM++L + FN P A
Sbjct 441 CIYHAVPLLDYTITGQDGQLLVTDAESLPIPEFDNIGMETLPMTQIFN--------SPKA 492
Query 542 SVM-----GYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLDPEYLSKWIDSTVSAG----Q 592
S++ GY PR+ + KT D + GAF +TLKSWV+P+ LS W S G Q
Sbjct 493 SIVNLFNAGYNPRYFNWKTKLDVINGAFTTTLKSWVSPVTESLLSGWFGFGYSEGDVNSQ 552
Query 593 TYYSLNYGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAVRNFDYDGMPY 647
LNY FFKVNPSVLD IF V ADS+ D+DQ L + Y+ RN DG+PY
Sbjct 553 NKVVLNYKFFKVNPSVLDPIFGVAADSTWDSDQLLVNSYIGCYVARNLSRDGVPY 607
> Alpavirinae_Human_feces_E_011_Microviridae_AG0388_putative.VP1
Length=622
Score = 421 bits (1081), Expect = 6e-140, Method: Compositional matrix adjust.
Identities = 254/658 (39%), Positives = 359/658 (55%), Gaps = 64/658 (10%)
Query 10 DVRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPVNTAAYTR 69
+++NHPH++GFD+ + FT+K GEL+PVY+ P + I FTRT+PV TAAYTR
Sbjct 9 ELQNHPHKAGFDVGSKNLFTAKVGELIPVYWDFAIPDCDYDIDLAYFTRTRPVQTAAYTR 68
Query 70 IREYLDWYFVPLRLINKNLPQALMNM-QDNPVQASGIVSNKIVTSDIPWTLLGHDSKPLG 128
+REY D+Y VP L+ K+ A++ M Q PVQ+ ++ V +DIPW L L
Sbjct 69 VREYFDFYAVPCDLLWKSFDSAVIQMGQTAPVQSKTLLDPLTVGNDIPWCTL------LD 122
Query 129 IANLLFRMYKGDSSAQIDPVL------NFFGFNSGTLGAKLAMMLRYGNFISKDYS-AST 181
++N ++ + SS V N FG+N G + +KL L YGNF++ S +
Sbjct 123 LSNAVY--FSSGSSPLGSTVSVPSGFGNIFGYNRGDVDSKLLFYLNYGNFVNPSLSNVGS 180
Query 182 PGDLSFGLSKSDFDLRAYNSSY----AVNILPFAVYQKIYADHFRFSQWEKNEPYTYNFD 237
P + + S S + Y+ Y AVNI P YQKIY D FR+SQWE +P +YN D
Sbjct 181 PSNRWWNTSFSSSKVPGYSQKYLNNNAVNIFPLLAYQKIYQDFFRWSQWENADPTSYNVD 240
Query 238 WYSG-GNVFNVFALGAADSTLKTYLEGNNLFTLRYANWPKDLFMGVMPNSQLGDVSVVDI 296
+Y+G GN+F L + + +Y + +N+F+LRY NW KD+F G++PNSQ GDV+VV++
Sbjct 241 YYNGSGNLFGSSGLSGSVTASNSYWKRDNMFSLRYCNWNKDMFTGLLPNSQFGDVAVVNL 300
Query 297 TTDSATRSYPVSLFSSA---SGPNGINKLGAAIPDAPFNEATDTPSTYNFSIDFGTEKWD 353
DS + + PV S + + +P + TP + S+ D
Sbjct 301 -GDSGSGTIPVGFLSDTEVFTQAFNATSMSTVSDTSPMGISGSTPVSARQSMVARINNAD 359
Query 354 SSIKAKTWvgvnnvgsgvlgvtvPGSQLAASFSVLQLRMAEAVQKYREVSQVADQTVRDQ 413
ASFS+L LR AEA+QK++E++Q D RDQ
Sbjct 360 ----------------------------VASFSILALRQAEALQKWKEITQSVDTNYRDQ 391
Query 414 IYAHFGVSLSPALSDTCFRVGGSASNIDISEVVNNNLAGENQADIMgkgvgtgqggTS-- 471
I AHFG++ ++S +GG A N+DISEVVNNNL + ++ G
Sbjct 392 IKAHFGINTPASMSHMAQYIGGVARNLDISEVVNNNLKDDGSEAVIYGKGVGSGSGKMRY 451
Query 472 FSSDEYGVLMAIYHVVPLLDYVITGQPHELLYTNTSDLPFPEFDSIGMQSLHFGRFFNYK 531
+ +Y ++M IYH +PLLDY ITGQ +LL T+ DLP PEFD+IGM+++ FN
Sbjct 452 HTGSQYCIIMCIYHAMPLLDYAITGQDPQLLCTSVEDLPIPEFDNIGMEAVPATTLFN-- 509
Query 532 SDKFTFDPTA--SVMGYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLDPEYLSKWIDSTVS 589
FD TA +GY PR+ K+ D V+GAF +TLK WVAP+D +YL W +S
Sbjct 510 --SVLFDGTAVNDFLGYNPRYWPWKSKIDRVHGAFTTTLKDWVAPIDDDYLHNWFNSKDG 567
Query 590 AGQTYYSLNYGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAVRNFDYDGMPY 647
S+++ FFKVNP+ LDSIF V ADS +TDQ L + + K VR DGMPY
Sbjct 568 KSA---SISWPFFKVNPNTLDSIFAVVADSIWETDQLLINCDVSCKVVRPLSQDGMPY 622
> Alpavirinae_Human_feces_D_015_Microviridae_AG019_putative.VP1
Length=584
Score = 407 bits (1045), Expect = 6e-135, Method: Compositional matrix adjust.
Identities = 253/652 (39%), Positives = 348/652 (53%), Gaps = 79/652 (12%)
Query 5 LFSFGDVRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPVNT 64
LF+ V+N P RSGFDLS ++ F++K GELLP+ + L PGDKF ++ Q FTRTQPVNT
Sbjct 3 LFNLSSVKNRPRRSGFDLSSKVAFSAKVGELLPIKWTLTMPGDKFSLKEQHFTRTQPVNT 62
Query 65 AAYTRIREYLDWYFVPLRLINKNLPQALMNMQDNPVQASGIVSNKIVTSDIPWTLLGHDS 124
+AYTR+REY DW++ PL L+ +N P+ + +Q N AS + ++ S++P S
Sbjct 63 SAYTRVREYYDWFWCPLHLLWRNAPEVIAQIQQNVQHASSFDGSVLLGSNMPCFSADQIS 122
Query 125 KPLGIANLLFRMYKGDSSAQIDPVLNFFGFNSGTLGAKLAMMLRYGNFISKDYSASTPGD 184
+ L + + LN+FGFN L KL LRYGN + T G
Sbjct 123 QSLDM---------------MKSKLNYFGFNRADLAYKLIQYLRYGNVRT---GVGTSGS 164
Query 185 LSFGLSKSDFDLRAYNS----SYAVNILPFAVYQKIYADHFRFSQWEKNEPYTYNFDWYS 240
++G S D +YN ++A+++ P Y+K D+FR +QW+ + PY +N D+Y
Sbjct 165 RNYGTS-VDVKDSSYNQNRAFNHALSVFPILAYKKFCQDYFRLTQWQVSAPYLWNIDYYD 223
Query 241 GGNVFNVFALGAADSTLKTYLEGNNLFTLRYANWPKDLFMGVMPNSQLGDVSVVDITTDS 300
G + + S TY E + F L Y NW KD+F G +P++Q GD SVVDI
Sbjct 224 GKGATTILPADLSKSV--TYFEHDTFFDLEYCNWNKDMFFGSLPDAQYGDTSVVDI---- 277
Query 301 ATRSYPVSLFSSASGPNGINKLGAAIPDAPFNEATDTPSTYNFSIDFGTEKWDSSIKAKT 360
SY G G + A +P N +T ++ FS I+A
Sbjct 278 ---SY---------GTTGAPVITAQNLQSPVNSSTAIGTSDKFSTQL--------IEA-- 315
Query 361 WvgvnnvgsgvlgvtvPGSQLAASFSVLQLRMAEAVQKYREVSQVADQTVRDQIYAHFGV 420
G+ L + VL LR EA+Q++RE+S R QI AHFGV
Sbjct 316 -----------------GTNL--TLDVLALRRGEALQRFREISLCTPLNYRSQIKAHFGV 356
Query 421 SLSPALSDTCFRVGGSASNIDISEVVNNNLAGENQADIMgkgvgtgqggTSFSSDEYGVL 480
+ LS +GG AS++DISEVVN N+ N+A I GKG+GTGQG F + ++GVL
Sbjct 357 DVGSELSGMSTYIGGEASSLDISEVVNTNITESNEALIAGKGIGTGQGNEEFYAKDWGVL 416
Query 481 MAIYHVVPLLDYVITGQPHELLYTNTSDLPFPEFDSIGMQSLHFGRFFNY-----KSDKF 535
M IYH VPLLDYVI+ +L + + P PE D+IG++ + + N +
Sbjct 417 MCIYHSVPLLDYVISAPDPQLFASMNTSFPVPELDAIGLEPITVAYYSNNPIELPSTGGI 476
Query 536 TFDPTASVMGYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLDPEYLSKWIDSTVSAGQTYY 595
T PT +V GY+PR+ KT D V GAF +T K WVAP+ PE S + + G
Sbjct 477 TDAPTTTV-GYLPRYYAWKTSIDYVLGAFTTTEKEWVAPITPELWSNMLKPLGTKGT--- 532
Query 596 SLNYGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAVRNFDYDGMPY 647
+NY FFKVNPS+LD IF V ADS DTD FL + D++ RN DYDGMPY
Sbjct 533 GINYNFFKVNPSILDPIFAVNADSYWDTDTFLINAAFDIRVARNLDYDGMPY 584
> Alpavirinae_Human_feces_A_034_Microviridae_AG0105_putative.VP1
Length=618
Score = 394 bits (1013), Expect = 8e-130, Method: Compositional matrix adjust.
Identities = 258/667 (39%), Positives = 360/667 (54%), Gaps = 72/667 (11%)
Query 3 SNLFSFGDVRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPV 62
S+LFS+GD++N P RSGFDLS + FT+K GELLPVY+K PGDKF I + FTRTQPV
Sbjct 2 SSLFSYGDIKNSPRRSGFDLSNKCAFTAKVGELLPVYWKFCLPGDKFNISQEWFTRTQPV 61
Query 63 NTAAYTRIREYLDWYFVPLRLINKNLPQALMNMQDNPVQASGIVSNKIVTSDIPWTLLGH 122
+T+A+TRIREY +W+FVPL L+ +N +A+M+M++ P A+ S+ ++PW
Sbjct 62 DTSAFTRIREYYEWFFVPLHLLYRNSNEAIMSMENQPNYAASGSSSISFNRNLPWV---- 117
Query 123 DSKPLGIANLLFRMYKGDSSAQIDPVLNFFGFNSGTLGAKLAMMLRYGNFISKDYSASTP 182
D + +A G+ + P NFFG + KL L YG ++P
Sbjct 118 DLSTINVA-------IGNVQSSTSP-KNFFGVSRSEGFKKLVSYLGYGE--------TSP 161
Query 183 GDLSFGLSKSDFDLRAYNSSYAVNILPFAVYQKIYADHFRFSQWEKNEPYTYNFDWYSGG 242
Y + + P YQKIY D++R SQWEK++P+TYN D+++G
Sbjct 162 --------------EKYVDNLRCSAFPLYAYQKIYQDYYRHSQWEKSKPWTYNCDFWNGE 207
Query 243 NVFNVFALGAADSTLKTYLEGNN--LFTLRYANWPKDLFMGVMPNSQLGDVSVVDITTDS 300
+ + A T++ + + N +F LRYANW KDL+MG +PNSQ GDV+ + + D+
Sbjct 208 DSTPITA------TVELFSQSPNDSVFELRYANWNKDLWMGSLPNSQFGDVAGISLGFDA 261
Query 301 ATRSYPVSLFSSASGP-----NGINKLGAAIPDAPFNEATDTPSTYNFSIDFGTEKWDSS 355
+T V+ + G G + +G +N P N + T + D +
Sbjct 262 STMKVGVTGTALVKGNMPVGYGGKDGMGIRSQSRLYN-----PVGINDAQQVTTVQQDVN 316
Query 356 IKAKTWvgvnnvgsgvlgvtvP-----------GSQLAASFSVLQLRMAEAVQKYREVSQ 404
K ++ + QL A FSVLQLR AEA+QK++E++Q
Sbjct 317 NKENGYLFATGTDAFGRISNAAKINGSELFAQLSGQLDAQFSVLQLRAAEALQKWKEIAQ 376
Query 405 VADQTVRDQIYAHFGVSLSPALSDTCFRVGGSASNIDISEVVNNNLAGENQADIMgkgvg 464
Q Q+ AHFGVS +P S R+ G +IDIS V N NL + +A I GKG+G
Sbjct 377 ANGQNYAAQVKAHFGVSTNPMQSHRSTRICGFDGSIDISAVENTNLTAD-EAIIRGKGLG 435
Query 465 tgqgg--TSFSSDEYGVLMAIYHVVPLLDYVITGQPHELLYTNTSD-LPFPEFDSIGMQS 521
+ + F+ +E+GV+M IYH PLLDYV TG +L+ T + P PEFDS+GM+S
Sbjct 436 GQRINDPSDFTCNEHGVIMCIYHATPLLDYVPTGPDLQLMSTVKGESWPVPEFDSLGMES 495
Query 522 LHFGRFFNYKSDKFTFDPTA-SVMGYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLDPEYL 580
L N K+ D A S GYVPR+I KT D V GAF TLKSW AP+D +Y+
Sbjct 496 LPMLSLVNSKA---IGDIVARSYAGYVPRYISWKTSIDVVRGAFTDTLKSWTAPVDSDYM 552
Query 581 SKWIDSTVSAGQTYYSLNYGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAVRNF 640
+ + + L+Y +FKVNPSVL+ IF V D S +TDQ L + DVK RN
Sbjct 553 HVFFGEVIPQEGSPI-LSYTWFKVNPSVLNPIFAVSVDGSWNTDQLLCNCQFDVKVARNL 611
Query 641 DYDGMPY 647
YDGMPY
Sbjct 612 SYDGMPY 618
> Alpavirinae_Human_gut_33_005_Microviridae_AG0184_putative.VP1
Length=618
Score = 382 bits (981), Expect = 5e-125, Method: Compositional matrix adjust.
Identities = 257/670 (38%), Positives = 351/670 (52%), Gaps = 78/670 (12%)
Query 3 SNLFSFGDVRNHPHRSGFDLSRRICFTSKAGELLPVYYKLVYPGDKFQIRHQLFTRTQPV 62
+ LFS+GD++N P RSGFDL + FT+K GELLPVY+K PGDKF I + FTRTQPV
Sbjct 2 AGLFSYGDIKNKPRRSGFDLGNKNAFTAKVGELLPVYWKFCMPGDKFHISQEWFTRTQPV 61
Query 63 NTAAYTRIREYLDWYFVPLRLINKNLPQALMNMQDNPVQASGIVSNKIVTSDIPWTLLGH 122
+T+A+TRIREY +W+FVPL L+ +N +A+M++++ P A+ + + +PW L
Sbjct 62 DTSAFTRIREYYEWFFVPLHLMYRNSNEAIMSLENQPNYAASGTQSIVFNRKLPWVDL-- 119
Query 123 DSKPLGIANLLFRMYKGDSSAQIDPVLNFFGFNSGTLGAKLAMMLRYGNFISKDYSASTP 182
+ I N+ Y N FGF + KL F S + P
Sbjct 120 QTLNDAITNVQASTYHN----------NMFGFARSSGFYKL--------FNSLGVGETDP 161
Query 183 GDLSFGLSKSDFDLRAYNSSYAVNILPFAVYQKIYADHFRFSQWEKNEPYTYNFDWYSGG 242
SK+ +LR ++ PF YQKIY+DH+R SQWE N+P+TYN D+++G
Sbjct 162 -------SKTLANLR-------ISAFPFYAYQKIYSDHYRNSQWEVNKPWTYNCDFWNGE 207
Query 243 NVFNVFALGAADSTLKTYLEGNNLFTLRYANWPKDLFMGVMPNSQLGDVSVVDITTDSAT 302
+ V A L +++F LRYANW KDL+MG MPN+Q GDV+ V + D++T
Sbjct 208 DSTPV----AFTKDLFDTNPNDSVFELRYANWNKDLYMGAMPNTQFGDVAAVSLGFDTST 263
Query 303 RSYPVSLFSSASG----------------------PNGINKLGAAIPDAPFNEATDTPST 340
++ + +G P GIN A E + +
Sbjct 264 MKIGITGTAPVTGNMPVGYGGKDGMGLRSQSRLYNPVGIND--AQQVTTVQEEVNNKENG 321
Query 341 YNFSIDFGTEKWDSSIKAKTWvgvnnvgsgvlgvtvPGSQLAASFSVLQLRMAEAVQKYR 400
Y F+ GT + A G + QL A FSVLQLR AE +QK++
Sbjct 322 YLFAT--GTNAFGRIFNAAKISGSDLNAQLS-------GQLDAKFSVLQLRAAECLQKWK 372
Query 401 EVSQVADQTVRDQIYAHFGVSLSPALSDTCFRVGGSASNIDISEVVNNNLAGENQADIMg 460
E++Q Q Q+ AHFGVS +P S RV G +IDIS V N NL+ + +A I G
Sbjct 373 EIAQANGQNYAAQVKAHFGVSPNPITSHRSTRVCGFDGSIDISAVENTNLSSD-EAIIRG 431
Query 461 kgvg--tgqggTSFSSDEYGVLMAIYHVVPLLDYVITGQPHELLYTNTSD-LPFPEFDSI 517
KG+G +F + E+GVLM IYH VPLLDY TG + + T D P PE DSI
Sbjct 432 KGIGGYRVNKPETFETTEHGVLMCIYHAVPLLDYAPTGPDLQFMTTVDGDSWPVPEMDSI 491
Query 518 GMQSLHFGRFFNYKSDKFTFDPTASVMGYVPRFIDLKTDYDEVYGAFRSTLKSWVAPLDP 577
G + L N + + +P GYVPR+I KT D V GAF TLKSW AP+
Sbjct 492 GFEELPSYSLLNTNAVQPIKEPRP--FGYVPRYISWKTSVDVVRGAFIDTLKSWTAPIGQ 549
Query 578 EYLSKWIDSTVSAGQTYYSLNYGFFKVNPSVLDSIFKVKADSSMDTDQFLSSLYLDVKAV 637
+YL + D+ G ++ Y +FKVNPSV++ IF V AD S +TDQ L + DV+
Sbjct 550 DYLKIYFDNNNVPGGAHFGF-YTWFKVNPSVVNPIFGVVADGSWNTDQLLVNCDFDVRVA 608
Query 638 RNFDYDGMPY 647
RN YDG+PY
Sbjct 609 RNLSYDGLPY 618
Lambda K H a alpha
0.319 0.135 0.407 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 60102543