bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Microviridae_proteins.fasta 575 sequences; 147,441 total letters Query= Contig-1_CDS_annotation_glimmer3.pl_2_7 Length=75 Score E Sequences producing significant alignments: (Bits) Value Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4 21.2 1.7 Gokush_Human_gut_33_003_Microviridae_AG061_putative.VP1 20.4 3.4 Gokush_Human_feces_E_009_Microviridae_AG0283_putative.VP2 20.0 4.3 Gokush_Human_feces_B_029_Microviridae_AG0416_putative.VP4 19.2 7.6 Gokush_Human_feces_E_007_Microviridae_AG0414_putative.VP4 18.9 9.4 > Alpavirinae_Human_feces_E_011_Microviridae_AG0385_putative.VP4 Length=611 Score = 21.2 bits (43), Expect = 1.7, Method: Compositional matrix adjust. Identities = 8/18 (44%), Positives = 11/18 (61%), Gaps = 0/18 (0%) Query 35 RSYLASNFPNYQSSVFRV 52 R YL + PN+ S FR+ Sbjct 397 RGYLGIDTPNFGSDDFRL 414 > Gokush_Human_gut_33_003_Microviridae_AG061_putative.VP1 Length=545 Score = 20.4 bits (41), Expect = 3.4, Method: Composition-based stats. Identities = 10/25 (40%), Positives = 11/25 (44%), Gaps = 0/25 (0%) Query 22 AYSGFRSNANRKFRSYLASNFPNYQ 46 AY G R KF Y S+ P Q Sbjct 182 AYKGGRCLPVNKFHDYFTSSLPFAQ 206 > Gokush_Human_feces_E_009_Microviridae_AG0283_putative.VP2 Length=294 Score = 20.0 bits (40), Expect = 4.3, Method: Composition-based stats. Identities = 10/38 (26%), Positives = 18/38 (47%), Gaps = 0/38 (0%) Query 29 NANRKFRSYLASNFPNYQSSVFRVLTYSSIMSMLHGYP 66 N R YL N+PN +++ L ++ S ++ P Sbjct 241 NLERYNEEYLRKNYPNTTAALLSSLYEATTGSKVNDDP 278 > Gokush_Human_feces_B_029_Microviridae_AG0416_putative.VP4 Length=292 Score = 19.2 bits (38), Expect = 7.6, Method: Composition-based stats. Identities = 9/23 (39%), Positives = 13/23 (57%), Gaps = 4/23 (17%) Query 13 YQKGYITIYAYSGFRSNANRKFR 35 Y GY+T AY N N+++R Sbjct 166 YVAGYVTKKAY----GNDNKRYR 184 > Gokush_Human_feces_E_007_Microviridae_AG0414_putative.VP4 Length=298 Score = 18.9 bits (37), Expect = 9.4, Method: Composition-based stats. Identities = 9/24 (38%), Positives = 14/24 (58%), Gaps = 2/24 (8%) Query 9 QVYYYQKGYIT--IYAYSGFRSNA 30 + Y Y GY+T +Y G ++NA Sbjct 167 ETYRYVAGYVTKKMYELDGKKANA 190 Lambda K H a alpha 0.323 0.133 0.399 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 3646980