bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Microviridae_proteins.fasta 575 sequences; 147,441 total letters Query= Contig-13_CDS_annotation_glimmer3.pl_2_5 Length=313 Score E Sequences producing significant alignments: (Bits) Value Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 394 2e-138 Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 378 7e-133 Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 372 1e-129 Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 358 2e-124 Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 354 9e-123 Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 293 3e-99 Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 238 6e-78 Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 224 3e-72 Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 223 3e-71 Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 219 5e-70 > Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 Length=332 Score = 394 bits (1011), Expect = 2e-138, Method: Compositional matrix adjust. Identities = 187/274 (68%), Positives = 229/274 (84%), Gaps = 1/274 (0%) Query 1 LRYVTAACGDCYECRKQKQRQWMVRMSEENRQTPNAYFLTLTIDDKSYKQIKQKYNLKDN 60 LRY+TAACG C ECR+QKQRQW+VRMSEE RQ PNAYF+TLTIDDKSY ++ YN+ DN Sbjct 31 LRYITAACGKCMECRQQKQRQWLVRMSEELRQEPNAYFITLTIDDKSYSELSNTYNITDN 90 Query 61 NDIATKAIRLCLERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGEKVTNNWKY 120 N+IATKAIRLCLER+RK TGKS+KHWFITELGHEKTERLHLHGIVWG+G + +T+ W Y Sbjct 91 NEIATKAIRLCLERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDKLITSKWNY 150 Query 121 GITFTGYFVNEKTIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAKRHVYIPGK 180 GITFTG+FVNEKTI+YITKYM K+DE+H F GKVLCS GIGAGY+KR+DAK+H Y G+ Sbjct 151 GITFTGFFVNEKTIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKRGE 210 Query 181 TNESYRMRNGEKLNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDLKTEELRYNG 240 T E+YR+RNG K+NLPIYYRN++FTEEERE LFLDKIEKGIIY++G K+ EE Y Sbjct 211 TIETYRLRNGSKINLPIYYRNQLFTEEEREALFLDKIEKGIIYVMGQKVHRDDEEY-YLQ 269 Query 241 VLASERERCERLYHDNPKEWDKRKYLNRVKKQRQ 274 +L R+ RLY N + W+++KYL+R+++Q++ Sbjct 270 LLDEGRKTECRLYGYNLQNWEQQKYLSRLRRQKK 303 > Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 Length=291 Score = 378 bits (971), Expect = 7e-133, Method: Compositional matrix adjust. Identities = 179/262 (68%), Positives = 220/262 (84%), Gaps = 1/262 (0%) Query 13 ECRKQKQRQWMVRMSEENRQTPNAYFLTLTIDDKSYKQIKQKYNLKDNNDIATKAIRLCL 72 ECR+QKQRQW+VRMSEE RQ PNAYF+TLTIDDKSY ++ YN+ DNN+IATKAIRLCL Sbjct 2 ECRQQKQRQWLVRMSEELRQEPNAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCL 61 Query 73 ERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGEKVTNNWKYGITFTGYFVNEK 132 ER+RK TGKS+KHWFITELGHEKTERLHLHGIVWG+G + +TN W YGITFTG+FVNEK Sbjct 62 ERIRKQTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDKLITNKWNYGITFTGFFVNEK 121 Query 133 TIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAKRHVYIPGKTNESYRMRNGEK 192 TI+YITKYM K+DE+H F GKVLCS GIGAGY+KR+DAK+H Y PG+T E+YR+RNG K Sbjct 122 TIQYITKYMTKIDEQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKPGETIETYRLRNGSK 181 Query 193 LNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDLKTEELRYNGVLASERERCERL 252 +NLPIYYRN++FTEEE+E LFLDKIEKGIIY++G K+ EE Y +L R+ RL Sbjct 182 INLPIYYRNQLFTEEEKEALFLDKIEKGIIYVMGQKVHRDDEEY-YLQLLNEGRKTECRL 240 Query 253 YHDNPKEWDKRKYLNRVKKQRQ 274 Y N + W+++KYL+R+++Q++ Sbjct 241 YGYNLQNWEQQKYLSRLRRQKK 262 > Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 Length=332 Score = 372 bits (954), Expect = 1e-129, Method: Compositional matrix adjust. Identities = 178/267 (67%), Positives = 213/267 (80%), Gaps = 1/267 (0%) Query 1 LRYVTAACGDCYECRKQKQRQWMVRMSEENRQTPNAYFLTLTIDDKSYKQIKQKYNLKDN 60 L YVTAACG C ECR+QKQRQW+VRMSEE RQ PNAYF+TLTIDD++Y ++ N KDN Sbjct 31 LLYVTAACGKCMECRQQKQRQWLVRMSEELRQNPNAYFMTLTIDDENYNKLANICNSKDN 90 Query 61 NDIATKAIRLCLERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGEKVTNNWKY 120 N+IATKA+RL LER+RK TGKS+KHWFITELGHEKTERLHLHGIVWG+G + ++ W Y Sbjct 91 NEIATKAVRLMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDQLISEKWNY 150 Query 121 GITFTGYFVNEKTIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAKRHVYIPGK 180 G +TG FVNE TI YITKYM KVD HP F G+VLCS GIGAGY KREDA H Y GK Sbjct 151 GFVYTGNFVNEATINYITKYMTKVDIDHPDFVGQVLCSKGIGAGYTKREDANNHKYTKGK 210 Query 181 TNESYRMRNGEKLNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDLKTEELRYNG 240 TNE+YR+RNG K+NLPIYYRN++F+EEERE LFLDKIEKGIIY++G K+ + E Y G Sbjct 211 TNETYRLRNGAKINLPIYYRNQLFSEEEREMLFLDKIEKGIIYVMGQKVH-RDNEAEYLG 269 Query 241 VLASERERCERLYHDNPKEWDKRKYLN 267 +L R+ +RLY + +EW+++KYLN Sbjct 270 LLEEARKTEQRLYGVHEQEWEEQKYLN 296 > Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 Length=332 Score = 358 bits (919), Expect = 2e-124, Method: Compositional matrix adjust. Identities = 175/272 (64%), Positives = 216/272 (79%), Gaps = 1/272 (0%) Query 3 YVTAACGDCYECRKQKQRQWMVRMSEENRQTPNAYFLTLTIDDKSYKQIKQKYNLKDNND 62 Y+TAACG C ECRKQKQR+W+VRMSEE R PNAYF+TLTI D++Y+ +K +D+N Sbjct 33 YITAACGKCLECRKQKQREWLVRMSEELRTEPNAYFMTLTISDENYEILKNICKSEDDNT 92 Query 63 IATKAIRLCLERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGEKVTNNWKYGI 122 IATKAIRL LER+RK TGKS+KHWFITELGHEKTERLHLHGIVWG+G + + W YGI Sbjct 93 IATKAIRLMLERIRKKTGKSIKHWFITELGHEKTERLHLHGIVWGIGTDQLIEEKWNYGI 152 Query 123 TFTGYFVNEKTIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAKRHVYIPGKTN 182 T+TG +VNEKTI Y+TKYM K+DEKHP F GKVLCS GIGAGY KR DA +H Y G+T Sbjct 153 TYTGNYVNEKTINYVTKYMTKIDEKHPDFVGKVLCSRGIGAGYTKRPDAAKHKYKKGETI 212 Query 183 ESYRMRNGEKLNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDLKTEELRYNGVL 242 E+YR+RNG K+NLPIYYRNK+FTE+ERE LF+DKIEKGIIY++G K+ EE Y +L Sbjct 213 ETYRLRNGAKINLPIYYRNKLFTEKERELLFIDKIEKGIIYVMGQKVHRDDEEY-YLQLL 271 Query 243 ASERERCERLYHDNPKEWDKRKYLNRVKKQRQ 274 R+ RLY N + W+K+KYL+R+++Q++ Sbjct 272 DEGRKTECRLYGYNLQNWEKQKYLSRLRRQKK 303 > Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 Length=332 Score = 354 bits (908), Expect = 9e-123, Method: Compositional matrix adjust. Identities = 171/267 (64%), Positives = 212/267 (79%), Gaps = 1/267 (0%) Query 3 YVTAACGDCYECRKQKQRQWMVRMSEENRQTPNAYFLTLTIDDKSYKQIKQKYNLKDNND 62 Y+TAACG C ECRKQKQR+W+VRMSEE R PNAYF+TLTI D++Y+ +K +D N Sbjct 33 YITAACGKCLECRKQKQREWLVRMSEELRTEPNAYFMTLTISDENYEILKNICKSEDENT 92 Query 63 IATKAIRLCLERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGEKVTNNWKYGI 122 IATKAIRL LER+RK GKS++HWFITELGHEKTERLHLHGIVWG+G + + W YGI Sbjct 93 IATKAIRLMLERIRKKIGKSIRHWFITELGHEKTERLHLHGIVWGIGTDQLIREKWNYGI 152 Query 123 TFTGYFVNEKTIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAKRHVYIPGKTN 182 T+TG FVNEKTI YITKYM K+DE+HP F GKVLCS GIGAGY KR DA +H Y G+T Sbjct 153 TYTGNFVNEKTINYITKYMTKIDEEHPNFVGKVLCSKGIGAGYTKRPDAAKHKYKKGETI 212 Query 183 ESYRMRNGEKLNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDLKTEELRYNGVL 242 E+YR+RNG K+NLPIYYRNK+FTE+ERE LF+DKI+KGIIY+LG K+ + +E Y +L Sbjct 213 ETYRLRNGAKINLPIYYRNKLFTEKERELLFIDKIDKGIIYVLGTKVH-RDDEKYYIQLL 271 Query 243 ASERERCERLYHDNPKEWDKRKYLNRV 269 R++ LY ++ +EW+++KYLNR+ Sbjct 272 EEGRKKENMLYGNHTQEWEQQKYLNRL 298 > Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 Length=304 Score = 293 bits (750), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 150/274 (55%), Positives = 195/274 (71%), Gaps = 3/274 (1%) Query 1 LRYVTAACGDCYECRKQKQRQWMVRMSEENRQTPNAYFLTLTIDDKSYKQIKQKYNLKDN 60 LRYVTAACG CYECRKQK R W VR+SEE R PNA F+TLTI D+S+++IK Y + Sbjct 31 LRYVTAACGKCYECRKQKGRAWQVRLSEEVRSDPNAIFVTLTISDESWEKIKNTYIQLSD 90 Query 61 NDIATKAIRLCLERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGEKVTNN-WK 119 D K +RL LERVRK T KS+KHW TE G TER HLHG++WG N E +T + W+ Sbjct 91 EDCIKKMVRLFLERVRKKTKKSLKHWLTTERGGTNTERYHLHGLIWG-ENTEALTKSLWQ 149 Query 120 YGITFTGYFVNEKTIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAKRHVYIPG 179 YG F G FVNE T+ YITKY+ K D+KH F LCSAGIG GYL R D++ + + G Sbjct 150 YGFVFIGTFVNECTVNYITKYITKTDKKHKDFEPITLCSAGIGKGYLSRSDSELNRFREG 209 Query 180 KTNESYRMRNGEKLNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDLKTEELRYN 239 KT E+YR+RNG KLNLPIYYRNK++T+EEREKLFL+KI+KG ++I G + ++K + Y+ Sbjct 210 KTTETYRLRNGTKLNLPIYYRNKLYTDEEREKLFLEKIKKGKVWICGRECNIKDWKT-YS 268 Query 240 GVLASERERCERLYHDNPKEWDKRKYLNRVKKQR 273 +L E+ + ++L+ D+P +WD KY R+ +QR Sbjct 269 QILKEEQIKAKQLHGDSPIDWDIAKYERRLARQR 302 > Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 Length=314 Score = 238 bits (608), Expect = 6e-78, Method: Compositional matrix adjust. Identities = 128/282 (45%), Positives = 182/282 (65%), Gaps = 13/282 (5%) Query 1 LRYVTAACGDCYECRKQKQRQWMVRMSEENRQTPNAYFLTLTIDDKSYKQI--------K 52 LRYV CG C ECRK+K R W +R++EE + PNA F+TLT ++++Y+++ K Sbjct 29 LRYVPTKCGKCIECRKEKARNWRIRLAEELKNNPNALFITLTFNEENYQRLSWELFKKSK 88 Query 53 QKYNLKDNNDIATKAIRLCLERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGE 112 N + N++ A+R LER+RK T KS++HW +TE G E R+HLHGIVW + Sbjct 89 DNLNYTEQNEMCKTAVRRWLERIRKKTKKSIRHWMVTEKG-EDYGRIHLHGIVWC---PK 144 Query 113 KVTNNWKYGITFTGYFVNEKTIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAK 172 + W YG T+ G +VNE TI Y+TKYMLK+ EK P FRGKV+CSAGIG Y +AK Sbjct 145 ERIEQWGYGYTYIGDYVNETTIAYVTKYMLKICEKWPDFRGKVMCSAGIGCRYETSYNAK 204 Query 173 RHVYIPGKTNESYRMRNGEKLNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDLK 232 R+ Y +T E+Y++ NG +L LP YY +KI+TEEEREKL++ K E+G YI G K+ Sbjct 205 RNRYRGKETKETYKLENGRELPLPKYYHDKIYTEEEREKLWIIKQERGYRYIAGEKVSTD 264 Query 233 TEELRYNGVLASERERCERLYHDNPKEWDKRKYLNRVKKQRQ 274 E ++ + ++R E+LY D P++W++ K R++K RQ Sbjct 265 NLE-EWDNLTKYYQKRAEQLYGDKPEDWEREKTKARLEKMRQ 305 > Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 Length=316 Score = 224 bits (570), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 122/277 (44%), Positives = 178/277 (64%), Gaps = 8/277 (3%) Query 1 LRYVTAACGDCYECRKQKQRQWMVRMSEENRQTPNA-YFLTLTIDDKSYKQIKQKYNLKD 59 L YV CG C ECR+QKQR W+VR+SEE R A F+TLT +++SYK++ K+ Sbjct 31 LFYVPVKCGKCIECRQQKQRAWIVRLSEELRSGKGAGLFVTLTFNEESYKELAAI--TKN 88 Query 60 NNDIATKAIRLCLERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGEKVTNNWK 119 ND+ A+ E R+ +++HW +TE+G + R+H+HGI+W + + WK Sbjct 89 ENDMCRLALYRMNENYRQKYKHTIRHWCVTEIGDDG--RIHIHGIMWCPASD--IERYWK 144 Query 120 YGITFTGYFVNEKTIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAKRHVYIPG 179 YG + G FVNE+TI YITKYMLK F KVLCS GIG YL R D+KR+ Y Sbjct 145 YGYIYIGRFVNEQTILYITKYMLKYTPVDKNFEPKVLCSKGIGINYLDRLDSKRNTYREN 204 Query 180 KTNESYRMRNGEKLNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDLKTEELRYN 239 T+ESY +R+G K+NLP YY+ KI+TEEEREKL+++K EKG YI+G K+ EE Y Sbjct 205 NTDESYMLRSGRKINLPDYYKRKIYTEEEREKLWIEKQEKGYRYIMGEKVSTDNEEKVYK 264 Query 240 GVLASERERCERLYHDNPKEWDKRKYLNRVKKQRQWI 276 ++ R++ + LY++ P+EWD+ K+ +K++++++ Sbjct 265 -LMEYWRKKAKELYNEKPQEWDREKHKKAIKRRKEYL 300 > Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 Length=376 Score = 223 bits (568), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 130/289 (45%), Positives = 180/289 (62%), Gaps = 25/289 (9%) Query 1 LRYVTAACGDCYECRKQKQRQWMVRMSEENRQTPNAYFLTLTIDDKSYKQIKQKYNLK-- 58 L Y+ CG C ECRK+KQR+W VR+ EE + YF TLTID ++ K+I+ LK Sbjct 31 LLYIPVKCGCCIECRKEKQREWRVRLEEE-MHSNFGYFTTLTIDQENIKKIESITGLKWE 89 Query 59 -DNNDIATKAIRLCLERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGEKVTNN 117 + N+IATKA+RL LER RK TGKS++HW +TELG EK R+HLHGI +G + E + + Sbjct 90 ENPNEIATKALRLFLERTRKDTGKSIRHWCVTELG-EKNNRIHLHGIFFGQKSAELIRKH 148 Query 118 WKYGITFTGYFVNEKTIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAKRHVYI 177 W YG F G + N K+I YITKYMLKVD KHP+F+ VL S+GIG GY+ R D Y+ Sbjct 149 WNYGFIFIGGYCNSKSINYITKYMLKVDIKHPEFKQIVLASSGIGKGYIDRLD-----YL 203 Query 178 PGKTN------ESYRMRNGEKLNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDL 231 K N +Y RNG K+ +P YY+NKIFTE+EREK++++ + +G+++I G K+ Sbjct 204 WQKQNYKNINVATYTFRNGTKMAMPKYYKNKIFTEKEREKMWINNLNRGLLWIYGEKVKA 263 Query 232 KTEELRYNGVLASERERCER----LYHDNPKEWDKRKYLNRVKKQRQWI 276 E + + RE ++ DNP W+ K + +KQR+ I Sbjct 264 DDWE-----TIDNLREYWQKYGRETMGDNPIAWNAMKERRKEEKQRRAI 307 > Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 Length=355 Score = 219 bits (558), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 132/317 (42%), Positives = 188/317 (59%), Gaps = 9/317 (3%) Query 1 LRYVTAACGDCYECRKQKQRQWMVRMSEENRQTPNAYFLTLTIDDKSYKQIKQKYNLKDN 60 LRY+ CG C+ECRK+K+ W VR E+ R+TP A F T T+ + Y IK+KYNLK + Sbjct 31 LRYIEVECGYCFECRKKKRNAWRVRNFEQLRETPTAIFFTGTVSPERYDYIKEKYNLKTD 90 Query 61 NDIATKAIRLCLERVRKLTGKSVKHWFITELGHEKTERLHLHGIVWGLGNGEK------V 114 N+I TK RL LER+RK TGKS+KHW +TE GH T R+HLHGI + + + Sbjct 91 NEIITKIHRLFLERIRKETGKSMKHWCVTEKGHTNTRRIHLHGIFYAPNGMTQFKLINIL 150 Query 115 TNNWKYGITFTGYFVNEKTIKYITKYMLKVDEKHPKFRGKVLCSAGIGAGYLKREDAKRH 174 NNW G + G + NEKTI Y++KYM K D +P++ GKVLCS G+GAGY+KR KRH Sbjct 151 RNNWIDGYCYNGKYCNEKTINYVSKYMTKKDMDNPEYTGKVLCSPGLGAGYVKR-IGKRH 209 Query 175 VYIPGKTNESYRMRNGEKLNLPIYYRNKIFTEEEREKLFLDKIEKGIIYILGIKIDLKTE 234 + T E Y R G + LP YY+ K+FTE++RE+L++ + G ++ K+ + E Sbjct 210 EWNEENTKEDYYTRQGTYIALPKYYKYKLFTEDQREQLWIYRENSGEKFVGNFKVKITDE 269 Query 235 ELR--YNGVLASERERCERLYHDNPKEWDKRKYLNRVKKQRQWIESKATMVAekekrkee 292 E YN + E R++ D+ KE +K NR K ++ + + KE RK+ Sbjct 270 ESEEYYNVLKKQHNEDGIRIHKDDIKEIIIKKLQNRRDKNKKSKAQRLFELYGKELRKKI 329 Query 293 rsekrLNNDIDLFANLY 309 ++ +L DI+ F +Y Sbjct 330 NNDIKLARDIEEFKKIY 346 Lambda K H a alpha 0.319 0.137 0.415 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 26543545