bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-25_CDS_annotation_glimmer3.pl_2_2
Length=314
Score E
Sequences producing significant alignments: (Bits) Value
Gokush_Human_feces_E_010_Microviridae_AG0123_putative.VP4 166 3e-50
Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4 166 3e-50
Gokush_Human_gut_33_023_Microviridae_AG0362_putative.VP4 160 4e-48
Gokush_Bourget_248_Microviridae_AG0249_putative.VP4 159 1e-47
Gokush_JCVI_001_Microviridae_AG021_putative.VP4 159 2e-47
Gokush_Bourget_504_Microviridae_AG0255_putative.VP4 155 3e-46
Gokush_gi|47566147|ref|YP_022485.1|_nonstructural_protein_[Chla... 153 4e-45
Gokush_gi|77020121|ref|YP_338244.1|_putative_replication_protei... 152 7e-45
Gokush_gi|17402855|ref|NP_510879.1|_hypothetical_protein_PhiCPG... 149 4e-44
Gokush_gi|9634955|ref|NP_054653.1|_nonstructural_protein_[Chlam... 150 1e-43
> Gokush_Human_feces_E_010_Microviridae_AG0123_putative.VP4
Length=305
Score = 166 bits (420), Expect = 3e-50, Method: Compositional matrix adjust.
Identities = 95/232 (41%), Positives = 134/232 (58%), Gaps = 19/232 (8%)
Query 58 QFFVPCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADEYLPHDMSVSMYEMQTF 117
+F + CG+CI CR+S S EWA R V E R+H+ N +TLTY D +LP D S+ Q F
Sbjct 37 EFKISCGQCIGCRLSKSREWAARCVVEARSHKSNMFLTLTYDDAHLPEDHSLHYEHFQLF 96
Query 118 LKRLRKEVQP---SAIRFFGCGEYGEQFLRPHYHMVVFGHDFSDRYLFGHDKKGTKLYRS 174
+KR+RK Q +RFF CGEYG++ RPHYH ++FG F D+ L+ ++G LYRS
Sbjct 97 MKRMRKYFQTRFGQQLRFFMCGEYGDKLGRPHYHAIIFGVTFVDKQLWS-IRRGNNLYRS 155
Query 175 PQLEKVWPKGFSSVCEVEFDVAKYVAIYLQKPPA--------DGRHRAFVNMSRNPGIGY 226
LE++WP GFSS+ V F+ A YVA Y+ K DG+ F + S PGIG+
Sbjct 156 ATLERLWPFGFSSIGAVNFETAAYVARYVTKKITGPLKLEHYDGKVAEFCHCSLKPGIGH 215
Query 227 ---QAIKPNLMETDKL-YQDGKYIHLPRYYLKVLERSYP---DQIADLKERR 271
+ ++ D+L D + P Y+ K+LERS ++I L+E+R
Sbjct 216 DFCEKYMTDIYTNDRLILSDKIMMSPPAYFDKLLERSDIVRYEEIKRLREKR 267
> Gokush_Human_feces_C_031_Microviridae_AG0432_putative.VP4
Length=305
Score = 166 bits (420), Expect = 3e-50, Method: Compositional matrix adjust.
Identities = 95/232 (41%), Positives = 135/232 (58%), Gaps = 19/232 (8%)
Query 58 QFFVPCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADEYLPHDMSVSMYEMQTF 117
+F +PCG+CI CR+S S EWA R V E ++H++N +TLTY D +LP D S+ Q F
Sbjct 37 EFKIPCGQCIGCRLSKSREWAARCVVEAKSHKNNMFLTLTYDDAHLPEDGSLHYEHFQLF 96
Query 118 LKRLRKEVQP---SAIRFFGCGEYGEQFLRPHYHMVVFGHDFSDRYLFGHDKKGTKLYRS 174
+KR+RK +RFF CGEYG++ RPHYH ++FG F D+ L+ ++G LYRS
Sbjct 97 MKRMRKYFMSRFGQQLRFFMCGEYGDKLGRPHYHAIIFGVTFVDKQLWS-IRRGNNLYRS 155
Query 175 PQLEKVWPKGFSSVCEVEFDVAKYVAIYLQKPPA--------DGRHRAFVNMSRNPGIGY 226
LEK+WP GFSS+ V F+ A YVA Y+ K DG+ F + S PGIG+
Sbjct 156 RTLEKLWPYGFSSIGAVNFETAAYVARYVTKKITGPLKLDHYDGKVAEFCHCSLKPGIGH 215
Query 227 ---QAIKPNLMETDKLYQDGK-YIHLPRYYLKVLERSYP---DQIADLKERR 271
+ ++ D+L K + P Y+ K+LERS ++I L+E+R
Sbjct 216 DFCEKYMTDIYTNDRLILSEKIMMSPPAYFDKLLERSDVVRFEEIKRLREKR 267
> Gokush_Human_gut_33_023_Microviridae_AG0362_putative.VP4
Length=305
Score = 160 bits (406), Expect = 4e-48, Method: Compositional matrix adjust.
Identities = 103/285 (36%), Positives = 152/285 (53%), Gaps = 40/285 (14%)
Query 42 FTGESMCLMPVKLAKYQFFVPCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADE 101
F G S L P+K +PCG+C+ CR+ S +WA R + E HNC +TLT+ D
Sbjct 26 FKGHSG-LEPIK-------IPCGQCLGCRLERSRQWATRCIHEASLWSHNCFLTLTFDDG 77
Query 102 YLPHDMSVSMYEMQTFLKRLRKEVQP------------SAIRFFGCGEYGEQFLRPHYHM 149
+L + S+ F+KR RK + IRFF CGEYGE + RPH+H
Sbjct 78 HLSRNGSLEPKIFVDFMKRFRKRFKGIDSTVNDDGEVIYPIRFFHCGEYGELYNRPHHHA 137
Query 150 VVFGHDFSDRYLFGHDKKGTKLYRSPQLEKVWPKGFSSVCEVEFDVAKYVAIY-LQKPPA 208
++F DFSD+ L+ + G LYRS LE +WP GF S+ V FD A YVA Y L+K
Sbjct 138 IIFNFDFSDKELW-MVRNGNYLYRSRSLESLWPFGFCSIGAVTFDSAAYVARYILKKVTG 196
Query 209 D-------GRHRAFVNMSRNPGIG---YQAIKPNLMETDKLY-QDGKYIHLPRYYLKVLE 257
D G+ +V MSR PGI + K N+ + DK+Y + G + PRYY K+ +
Sbjct 197 DNSVTHYSGKIPEYVTMSRRPGIARDWFLKYKDNIYDNDKVYIKPGLVVKPPRYYDKIYD 256
Query 258 RSYPDQIADLKERRINHA-----ISEYVEMMANLKHHITQIEYRK 297
PD++ ++KE+R A I +Y + N+++H+ ++ ++
Sbjct 257 EINPDRMLEVKEKRYQIAQSCSPICDYQRL--NVRNHLKALKIKQ 299
> Gokush_Bourget_248_Microviridae_AG0249_putative.VP4
Length=299
Score = 159 bits (403), Expect = 1e-47, Method: Compositional matrix adjust.
Identities = 92/261 (35%), Positives = 134/261 (51%), Gaps = 24/261 (9%)
Query 61 VPCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADEYLPHDMSVSMYEMQTFLKR 120
+PCG+C+ CR+ S +WA R + E + H NC ITLTY D+++P D S+ + Q F+KR
Sbjct 33 LPCGQCVGCRLERSRQWAIRCMHEAQMHTQNCFITLTYNDDHIPSDRSLHYRDFQLFIKR 92
Query 121 LRKEVQPSAIRFFGCGEYGEQFLRPHYHMVVFGHDFSDRYLFGHDKKGTKLYRSPQLEKV 180
LRK IR++ GEYGE RPH+H +FG DF D+ L+ + LYRS LE +
Sbjct 93 LRKRYPGRRIRYYMAGEYGENLGRPHWHACIFGLDFDDKKLWKRTAANSILYRSKNLELL 152
Query 181 WPKGFSSVCEVEFDVAKYVAIYLQK--------------PPADG----RHRAFVNMSRNP 222
WP G+SS+ +V F+ A YVA Y+ K P G R F MS P
Sbjct 153 WPFGYSSIGDVTFESAAYVARYIMKKVTGKNAEQHYTEIDPESGEITIRKPEFTKMSLKP 212
Query 223 GIGYQAIK---PNLMETDKLYQDGKYIHLPRYYLKVLERSYP---DQIADLKERRINHAI 276
GIGY+ K ++ D + GK + P+YY K + P D++ +E+
Sbjct 213 GIGYEWYKKYTSDVYPHDYVVIRGKKVKPPKYYDKNYKIDNPYEFDELLYFREKSAKLNY 272
Query 277 SEYVEMMANLKHHITQIEYRK 297
+ +K +TQ + +K
Sbjct 273 EDNTPERLLVKEQVTQAKLQK 293
> Gokush_JCVI_001_Microviridae_AG021_putative.VP4
Length=297
Score = 159 bits (401), Expect = 2e-47, Method: Compositional matrix adjust.
Identities = 93/257 (36%), Positives = 131/257 (51%), Gaps = 28/257 (11%)
Query 46 SMCLMPVKLAKYQFFVPCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADEYLPH 105
S+ K A +PCG+CI CRI S WA R + E +E+NC +TLTY D++LP
Sbjct 16 SVSFSAPKGAGDSLTLPCGRCIGCRIDRSKMWAVRCLHEASLYENNCFVTLTYNDDHLPM 75
Query 106 DMSVSMYEMQTFLKRLRKEVQPSAIRFFGCGEYGEQFLRPHYHMVVFGHDFSDRYLFGHD 165
D S+ + Q F+KRLRK + S IRF+ CGEYGE+ RPH+H+++F DF DR L+
Sbjct 76 DGSLDYVDFQLFMKRLRKRFRGSKIRFYMCGEYGEKDARPHFHVILFNFDFEDRTLWKKS 135
Query 166 KKGTKLYRSPQLEKVWPKGFSSVCEVEFDVAKYVAIYLQKP--------------PADGR 211
G +YRS LE++WP GFSS+ V A YVA Y+ K P G
Sbjct 136 GSGEMIYRSAALEELWPFGFSSIGNVTMQSAGYVARYVMKKMTGDFAKKWYERVNPHTGE 195
Query 212 ----HRAFVNMSRNPGIG-------YQAIKPNLMETDKLYQDGKYIHLPRYYLKVLERSY 260
F MS PGI ++ + P E + + G+ + P++Y E+
Sbjct 196 LTRLKPEFNKMSLKPGIAQAWFDKHWKDVYP---EDAVVLEGGRRMRPPKFYDLKYEKLD 252
Query 261 PDQIADLKERRINHAIS 277
P DLK +R + +
Sbjct 253 PFGFEDLKFKRYKRSTA 269
> Gokush_Bourget_504_Microviridae_AG0255_putative.VP4
Length=299
Score = 155 bits (393), Expect = 3e-46, Method: Compositional matrix adjust.
Identities = 89/228 (39%), Positives = 118/228 (52%), Gaps = 21/228 (9%)
Query 61 VPCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADEYLPHDMSVSMYEMQTFLKR 120
+PCG+C+ CR+ S +WA R + E + H NC ITLTY D+ LP D S+ Q F+KR
Sbjct 33 LPCGQCVGCRLERSRQWAIRCMHEAQMHTQNCFITLTYNDDNLPSDKSLCYRHFQLFIKR 92
Query 121 LRKEVQPSAIRFFGCGEYGEQFLRPHYHMVVFGHDFSDRYLFGHDKKGTKLYRSPQLEKV 180
LRK IR++ GEYGE F RPH+H +FG DF D+ L+ + LY S LE +
Sbjct 93 LRKRYFGLKIRYYMAGEYGENFGRPHFHACLFGIDFDDKKLWKRTSANSMLYTSRDLEIL 152
Query 181 WPKGFSSVCEVEFDVAKYVAIYLQKP--------------PADG----RHRAFVNMSRNP 222
WP G+SSV V F+ A YVA Y+ K P G R F MS P
Sbjct 153 WPFGYSSVGNVTFESAAYVARYIMKKVTGKNAKDHYTEINPETGEILTRKPEFTKMSLKP 212
Query 223 GIGYQAIK---PNLMETDKLYQDGKYIHLPRYYLKVLERSYPDQIADL 267
GIGY K ++ D + GK + P+YY K + P + +L
Sbjct 213 GIGYSWYKKYTSDVYPNDYVVIRGKKVKPPKYYDKNYKIDNPYEFDEL 260
> Gokush_gi|47566147|ref|YP_022485.1|_nonstructural_protein_[Chlamydia_phage_3]
Length=315
Score = 153 bits (386), Expect = 4e-45, Method: Compositional matrix adjust.
Identities = 94/246 (38%), Positives = 139/246 (57%), Gaps = 18/246 (7%)
Query 62 PCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADEYLPHDMSVSMYEMQTFLKRL 121
PC KC CR+ ++ W+YR + E + NC +TLTY D +LP + S+ + FL+RL
Sbjct 54 PCLKCRFCRVQNAKIWSYRCMHEASLYSQNCFLTLTYEDRHLPENGSLVRDHPRLFLRRL 113
Query 122 RKEVQPSAIRFFGCGEYGEQFLRPHYHMVVFGHDFSDRYLFGHDKKGTKLYRSPQLEKVW 181
R+ + P IR+FGCGEYG + RPHYH++++ +DF D+ L K+G L+ S +L ++W
Sbjct 114 REHIYPHKIRYFGCGEYGSKLQRPHYHLLIYNYDFPDKKLLSK-KRGNPLFVSEKLMQLW 172
Query 182 PKGFSSVCEVEFDVAKYVAIY-LQKPPADG-------RHRAFVNMSRNPGIG---YQAIK 230
P GFS+V V A YVA Y L+K D R F+ S PGIG Y+ K
Sbjct 173 PFGFSTVGSVTRQSAGYVARYSLKKVNGDSSQDHYGQRLPEFLMCSLKPGIGADWYEKYK 232
Query 231 PNLMETDKL-YQD-GKYIHL--PRYYLKVLERSYPDQIADLKERRINH--AISEYVEMMA 284
++ D L QD GK PRYY K+ R P+++ ++K+RR+ A+ E + A
Sbjct 233 RDVYPQDYLVVQDKGKSFKTRPPRYYDKLHSRFDPEEMEEIKQRRVEKFMALPELTQDKA 292
Query 285 NLKHHI 290
+K +I
Sbjct 293 EVKQYI 298
> Gokush_gi|77020121|ref|YP_338244.1|_putative_replication_protein_[Chlamydia_phage_4]
Length=315
Score = 152 bits (385), Expect = 7e-45, Method: Compositional matrix adjust.
Identities = 94/257 (37%), Positives = 143/257 (56%), Gaps = 18/257 (7%)
Query 51 PVKLAKYQFFVPCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADEYLPHDMSVS 110
P + K +PC +C CR+ ++ W+YR + E + NC +TLTY D++LP + S+
Sbjct 43 PEEYRKRWILMPCRRCKFCRVQNAKIWSYRCMHEASLYSQNCFLTLTYEDQHLPENGSLV 102
Query 111 MYEMQTFLKRLRKEVQPSAIRFFGCGEYGEQFLRPHYHMVVFGHDFSDRYLFGHDKKGTK 170
FL+RLR+ + P IR+FGCGEYG + RPHYH++++ +DF D+ L K+G
Sbjct 103 RNHPTLFLRRLREHISPHKIRYFGCGEYGSKLQRPHYHLLIYNYDFPDKKLLSK-KRGNP 161
Query 171 LYRSPQLEKVWPKGFSSVCEVEFDVAKYVAIY-LQKPPAD-------GRHRAFVNMSRNP 222
L+ S +L ++WP GFS+V V A YVA Y L+K D R F+ S P
Sbjct 162 LFVSEKLMQLWPYGFSTVGSVTRQSAGYVARYSLKKVSRDISQDHYGQRLPEFLMCSLKP 221
Query 223 GIG---YQAIKPNLMETDKL-YQD-GKYIHL--PRYYLKVLERSYPDQIADLKERRINH- 274
GIG Y+ K ++ D L QD GK PRYY K+ R P+++ ++K++R+
Sbjct 222 GIGADWYEKYKRDVYPQDYLVVQDKGKSFTTRPPRYYDKLHSRFDPEEMDEVKQKRVEKV 281
Query 275 -AISEYVEMMANLKHHI 290
A+ E + A +K +I
Sbjct 282 MALPELSQDKAEVKQYI 298
> Gokush_gi|17402855|ref|NP_510879.1|_hypothetical_protein_PhiCPG1p9_[Guinea_pig_Chlamydia_phage]
Length=263
Score = 149 bits (376), Expect = 4e-44, Method: Compositional matrix adjust.
Identities = 94/247 (38%), Positives = 138/247 (56%), Gaps = 18/247 (7%)
Query 61 VPCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADEYLPHDMSVSMYEMQTFLKR 120
+P KC CR+ ++ W+YR + E + NC +TLTY D +LP + S+ FL R
Sbjct 1 MPWRKCKFCRVQNAKIWSYRCIHEASLYSQNCFLTLTYEDRHLPENGSLVRDHPALFLMR 60
Query 121 LRKEVQPSAIRFFGCGEYGEQFLRPHYHMVVFGHDFSDRYLFGHDKKGTKLYRSPQLEKV 180
LRKE+ P IR+FGCGEYG + RPHYH++++ +DF D+ L K+G L+ S +L ++
Sbjct 61 LRKEIYPHKIRYFGCGEYGSKLQRPHYHLLIYNYDFPDKKLLSK-KRGNPLFVSEKLMRL 119
Query 181 WPKGFSSVCEVEFDVAKYVAIY-LQKPPAD-------GRHRAFVNMSRNPGIG---YQAI 229
WP GFS+V V A YVA Y L+K D R F+ S PGIG Y+
Sbjct 120 WPFGFSTVGSVMRQSAGYVARYSLKKVNGDISQDHYGQRLPQFLMCSLKPGIGADWYEKY 179
Query 230 KPNLMETDKL-YQD-GKYIHL--PRYYLKVLERSYPDQIADLKERRINH--AISEYVEMM 283
K ++ D L QD GK PRYY K+ R P+++ ++K++RI A+ + +
Sbjct 180 KRDVYPQDYLVVQDKGKSFTTRPPRYYDKLHSRFDPEEMDEVKQKRIEKVMALPQLTQDK 239
Query 284 ANLKHHI 290
A +K +I
Sbjct 240 AEVKQYI 246
> Gokush_gi|9634955|ref|NP_054653.1|_nonstructural_protein_[Chlamydia_phage_2]
Length=336
Score = 150 bits (378), Expect = 1e-43, Method: Compositional matrix adjust.
Identities = 93/248 (38%), Positives = 139/248 (56%), Gaps = 18/248 (7%)
Query 60 FVPCGKCIECRISHSIEWAYRVVAETRAHEHNCMITLTYADEYLPHDMSVSMYEMQTFLK 119
+PC +C CR+ ++ W+YR + E + NC +TLTY D +LP + S+ + FL
Sbjct 73 LMPCRRCKFCRVQNAKIWSYRCMHEASLYSQNCFLTLTYEDRHLPENGSLVRDHPRLFLM 132
Query 120 RLRKEVQPSAIRFFGCGEYGEQFLRPHYHMVVFGHDFSDRYLFGHDKKGTKLYRSPQLEK 179
RLR+ + P IR+FGCGEYG + RPHYH++++ +DF D+ L K+G L+ S +L +
Sbjct 133 RLREHIYPHKIRYFGCGEYGSKLQRPHYHLLIYNYDFPDKKLLSK-KRGNPLFVSEKLMR 191
Query 180 VWPKGFSSVCEVEFDVAKYVAIY-LQKPPAD-------GRHRAFVNMSRNPGIG---YQA 228
+WP GFS+V V A YVA Y L+K D R F+ S PGIG Y+
Sbjct 192 LWPFGFSTVGSVTRQSAGYVARYSLKKVNGDISQDHYGQRLPEFLMCSLKPGIGADWYEK 251
Query 229 IKPNLMETDKL-YQD-GKYIHL--PRYYLKVLERSYPDQIADLKERRINH--AISEYVEM 282
K ++ D L QD GK PRYY K+ R P+++ ++K++RI A+ E +
Sbjct 252 YKCDVYPQDYLVVQDKGKSFKTRPPRYYDKLHSRFDPEEMDEVKQKRIEKVMALPELTQD 311
Query 283 MANLKHHI 290
A +K +I
Sbjct 312 KAEVKQYI 319
Lambda K H a alpha
0.327 0.140 0.441 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 26651886