bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for composition-based statistics: Alejandro A. Schaffer, L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Database: Microviridae_proteins.fasta 575 sequences; 147,441 total letters Query= Contig-20_CDS_annotation_glimmer3.pl_2_3 Length=101 Score E Sequences producing significant alignments: (Bits) Value Alpavirinae_Human_feces_C_010_Microviridae_AG0200_hypothetical.... 42.0 4e-08 Alpavirinae_Human_gut_21_005_Microviridae_AG014_hypothetical.pr... 40.8 1e-07 Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 23.5 0.41 Alpavirinae_Human_gut_31_037_Microviridae_AG0298_hypothetical.p... 20.4 4.5 Gokush_Human_feces_C_031_Microviridae_AG0434_hypothetical.prote... 20.4 4.6 > Alpavirinae_Human_feces_C_010_Microviridae_AG0200_hypothetical.protein Length=127 Score = 42.0 bits (97), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 20/70 (29%), Positives = 40/70 (57%), Gaps = 3/70 (4%) Query 30 GLSITPSDIERLARQGVPVSVPNANSFYSI--DSGLEVPPELKVDADRNSLWEMSQQSKA 87 L +TP + +A++G+PVS N N F + D+ + P+ + D D + WEM + S+ Sbjct 48 NLGLTPQQVAEMAKRGIPVSPMNVN-FIDVNGDASWNIEPQFRRDMDMATAWEMEKASQR 106 Query 88 RIMKARKREK 97 + ++ +++K Sbjct 107 KALQVLRQKK 116 > Alpavirinae_Human_gut_21_005_Microviridae_AG014_hypothetical.protein Length=107 Score = 40.8 bits (94), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 20/70 (29%), Positives = 39/70 (56%), Gaps = 3/70 (4%) Query 30 GLSITPSDIERLARQGVPVSVPNANSFYSI--DSGLEVPPELKVDADRNSLWEMSQQSKA 87 L +TP + +A++G+PVS N N F + D+ + P+ D D + WEM + S+ Sbjct 28 NLGLTPQQVAEMAKRGIPVSPMNVN-FIDVNGDASWNIEPQFCRDMDMATAWEMEKASQR 86 Query 88 RIMKARKREK 97 + ++ +++K Sbjct 87 KALQVLRQKK 96 > Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 Length=314 Score = 23.5 bits (49), Expect = 0.41, Method: Composition-based stats. Identities = 12/50 (24%), Positives = 24/50 (48%), Gaps = 6/50 (12%) Query 57 YSIDSGLEVP------PELKVDADRNSLWEMSQQSKARIMKARKREKDHL 100 Y +++G E+P ++ + +R LW + Q+ R + K D+L Sbjct 217 YKLENGRELPLPKYYHDKIYTEEEREKLWIIKQERGYRYIAGEKVSTDNL 266 > Alpavirinae_Human_gut_31_037_Microviridae_AG0298_hypothetical.protein Length=115 Score = 20.4 bits (41), Expect = 4.5, Method: Compositional matrix adjust. Identities = 10/27 (37%), Positives = 16/27 (59%), Gaps = 2/27 (7%) Query 75 RNSLWEMSQQSKARIMKA--RKREKDH 99 R WE++Q++ MKA KR+ D+ Sbjct 62 RTDRWEIAQKAMEENMKAISAKRKHDY 88 > Gokush_Human_feces_C_031_Microviridae_AG0434_hypothetical.protein.BACEGG.02723 Length=206 Score = 20.4 bits (41), Expect = 4.6, Method: Compositional matrix adjust. Identities = 8/30 (27%), Positives = 15/30 (50%), Gaps = 0/30 (0%) Query 54 NSFYSIDSGLEVPPELKVDADRNSLWEMSQ 83 NS D+GL+ + D+ N +W ++ Sbjct 103 NSALQADAGLKRAQSVAADSSSNLMWSQTK 132 Lambda K H a alpha 0.312 0.128 0.355 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 5045304