bitscore colors: <40, 40-50 , 50-80, 80-200, >200
BLASTP 2.2.30+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Reference for composition-based statistics: Alejandro A. Schaffer,
L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri
I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids
Res. 29:2994-3005.
Database: Microviridae_proteins.fasta
575 sequences; 147,441 total letters
Query= Contig-14_CDS_annotation_glimmer3.pl_2_4
Length=270
Score E
Sequences producing significant alignments: (Bits) Value
Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4 328 3e-113
Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4 311 1e-106
Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4 308 2e-105
Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4 306 1e-104
Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4 301 2e-103
Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4 226 1e-73
Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4 184 3e-57
Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4 172 5e-53
Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4 167 2e-50
Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4 147 6e-43
> Alpavirinae_Human_feces_A_048_Microviridae_AG086_putative.VP4
Length=332
Score = 328 bits (841), Expect = 3e-113, Method: Compositional matrix adjust.
Identities = 183/277 (66%), Positives = 217/277 (78%), Gaps = 7/277 (3%)
Query 1 MSEELRHDPNAYFMTLTFSEEKLEEYKKLCNSEDPNTIATKAMRLMLERCRRKLKHSIKH 60
MSEELR +PNAYFMTLT S+E E K +C SED NTIATKA+RLMLER R+K SIKH
Sbjct 56 MSEELRTEPNAYFMTLTISDENYEILKNICKSEDDNTIATKAIRLMLERIRKKTGKSIKH 115
Query 61 WFITELGHNGTERMHLHGLVWGIGMDKLVEEKWQNGIVFTGTFVNEktinyitkyitktD 120
WFITELGH TER+HLHG+VWGIG D+L+EEKW GI +TG +VNEKTINY+TKY+TK D
Sbjct 116 WFITELGHEKTERLHLHGIVWGIGTDQLIEEKWNYGITYTGNYVNEKTINYVTKYMTKID 175
Query 121 EYHKEFIGQILCSAGIGNGYTQRSDAQKHTYKKGETIETYRLRNGAKINLPTYYRNKLFT 180
E H +F+G++LCS GIG GYT+R DA KH YKKGETIETYRLRNGAKINLP YYRNKLFT
Sbjct 176 EKHPDFVGKVLCSRGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFT 235
Query 181 EEEREKLWIDKIEKGIVWVMGQKVKIDNTEEYEALLEQARHDAIRLQGHQEQNWDKQKYF 240
E+ERE L+IDKIEKGI++VMGQKV D+ E Y LL++ R RL G+ QNW+KQKY
Sbjct 236 EKERELLFIDKIEKGIIYVMGQKVHRDDEEYYLQLLDEGRKTECRLYGYNLQNWEKQKYL 295
Query 241 krlekqrkkqqkelrkWENH-------MFQSTEECPF 270
RL +Q+KKQ KEL++ E + + S +ECPF
Sbjct 296 SRLRRQKKKQDKELQQLETYWAINRAKKYTSLDECPF 332
> Alpavirinae_Human_feces_B_021_Microviridae_AG0372_putative.VP4
Length=332
Score = 311 bits (797), Expect = 1e-106, Method: Compositional matrix adjust.
Identities = 176/277 (64%), Positives = 213/277 (77%), Gaps = 7/277 (3%)
Query 1 MSEELRHDPNAYFMTLTFSEEKLEEYKKLCNSEDPNTIATKAMRLMLERCRRKLKHSIKH 60
MSEELR +PNAYFMTLT S+E E K +C SED NTIATKA+RLMLER R+K+ SI+H
Sbjct 56 MSEELRTEPNAYFMTLTISDENYEILKNICKSEDENTIATKAIRLMLERIRKKIGKSIRH 115
Query 61 WFITELGHNGTERMHLHGLVWGIGMDKLVEEKWQNGIVFTGTFVNEktinyitkyitktD 120
WFITELGH TER+HLHG+VWGIG D+L+ EKW GI +TG FVNEKTINYITKY+TK D
Sbjct 116 WFITELGHEKTERLHLHGIVWGIGTDQLIREKWNYGITYTGNFVNEKTINYITKYMTKID 175
Query 121 EYHKEFIGQILCSAGIGNGYTQRSDAQKHTYKKGETIETYRLRNGAKINLPTYYRNKLFT 180
E H F+G++LCS GIG GYT+R DA KH YKKGETIETYRLRNGAKINLP YYRNKLFT
Sbjct 176 EEHPNFVGKVLCSKGIGAGYTKRPDAAKHKYKKGETIETYRLRNGAKINLPIYYRNKLFT 235
Query 181 EEEREKLWIDKIEKGIVWVMGQKVKIDNTEEYEALLEQARHDAIRLQGHQEQNWDKQKYF 240
E+ERE L+IDKI+KGI++V+G KV D+ + Y LLE+ R L G+ Q W++QKY
Sbjct 236 EKERELLFIDKIDKGIIYVLGTKVHRDDEKYYIQLLEEGRKKENMLYGNHTQEWEQQKYL 295
Query 241 krlekqrkkqqkelrkWENHM-------FQSTEECPF 270
RL+KQ+KKQ++E+ + E + + EECPF
Sbjct 296 NRLQKQKKKQEQEINQLETYWALERAKDYTQLEECPF 332
> Alpavirinae_Human_feces_B_020_Microviridae_AG0349_putative.VP4
Length=332
Score = 308 bits (789), Expect = 2e-105, Method: Compositional matrix adjust.
Identities = 160/240 (67%), Positives = 187/240 (78%), Gaps = 0/240 (0%)
Query 1 MSEELRHDPNAYFMTLTFSEEKLEEYKKLCNSEDPNTIATKAMRLMLERCRRKLKHSIKH 60
MSEELR +PNAYFMTLT +E + +CNS+D N IATKA+RLMLER R+K SIKH
Sbjct 56 MSEELRQNPNAYFMTLTIDDENYNKLANICNSKDNNEIATKAVRLMLERIRKKTGKSIKH 115
Query 61 WFITELGHNGTERMHLHGLVWGIGMDKLVEEKWQNGIVFTGTFVNEktinyitkyitktD 120
WFITELGH TER+HLHG+VWGIG D+L+ EKW G V+TG FVNE TINYITKY+TK D
Sbjct 116 WFITELGHEKTERLHLHGIVWGIGTDQLISEKWNYGFVYTGNFVNEATINYITKYMTKVD 175
Query 121 EYHKEFIGQILCSAGIGNGYTQRSDAQKHTYKKGETIETYRLRNGAKINLPTYYRNKLFT 180
H +F+GQ+LCS GIG GYT+R DA H Y KG+T ETYRLRNGAKINLP YYRN+LF+
Sbjct 176 IDHPDFVGQVLCSKGIGAGYTKREDANNHKYTKGKTNETYRLRNGAKINLPIYYRNQLFS 235
Query 181 EEEREKLWIDKIEKGIVWVMGQKVKIDNTEEYEALLEQARHDAIRLQGHQEQNWDKQKYF 240
EEERE L++DKIEKGI++VMGQKV DN EY LLE+AR RL G EQ W++QKY
Sbjct 236 EEEREMLFLDKIEKGIIYVMGQKVHRDNEAEYLGLLEEARKTEQRLYGVHEQEWEEQKYL 295
> Alpavirinae_Human_feces_A_047_Microviridae_AG0312_putative.VP4
Length=332
Score = 306 bits (783), Expect = 1e-104, Method: Compositional matrix adjust.
Identities = 170/277 (61%), Positives = 209/277 (75%), Gaps = 7/277 (3%)
Query 1 MSEELRHDPNAYFMTLTFSEEKLEEYKKLCNSEDPNTIATKAMRLMLERCRRKLKHSIKH 60
MSEELR +PNAYF+TLT ++ E N D N IATKA+RL LER R++ SIKH
Sbjct 56 MSEELRQEPNAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCLERIRKQTGKSIKH 115
Query 61 WFITELGHNGTERMHLHGLVWGIGMDKLVEEKWQNGIVFTGTFVNEktinyitkyitktD 120
WFITELGH TER+HLHG+VWGIG DKL+ KW GI FTG FVNEKTI YITKY+TK D
Sbjct 116 WFITELGHEKTERLHLHGIVWGIGTDKLITSKWNYGITFTGFFVNEKTIQYITKYMTKID 175
Query 121 EYHKEFIGQILCSAGIGNGYTQRSDAQKHTYKKGETIETYRLRNGAKINLPTYYRNKLFT 180
E HK+FIG++LCS GIG GY +R DA+KHTYK+GETIETYRLRNG+KINLP YYRN+LFT
Sbjct 176 EQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKRGETIETYRLRNGSKINLPIYYRNQLFT 235
Query 181 EEEREKLWIDKIEKGIVWVMGQKVKIDNTEEYEALLEQARHDAIRLQGHQEQNWDKQKYF 240
EEERE L++DKIEKGI++VMGQKV D+ E Y LL++ R RL G+ QNW++QKY
Sbjct 236 EEEREALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLDEGRKTECRLYGYNLQNWEQQKYL 295
Query 241 krlekqrkkqqkelrkWENHM-------FQSTEECPF 270
RL +Q+K+Q K+L++ E + + S ++CPF
Sbjct 296 SRLRRQKKRQDKDLQQLETYWAIEKSKEYTSLDKCPF 332
> Alpavirinae_Human_feces_C_029_Microviridae_AG0108_putative.VP4
Length=291
Score = 301 bits (771), Expect = 2e-103, Method: Compositional matrix adjust.
Identities = 169/277 (61%), Positives = 205/277 (74%), Gaps = 7/277 (3%)
Query 1 MSEELRHDPNAYFMTLTFSEEKLEEYKKLCNSEDPNTIATKAMRLMLERCRRKLKHSIKH 60
MSEELR +PNAYF+TLT ++ E N D N IATKA+RL LER R++ SIKH
Sbjct 15 MSEELRQEPNAYFITLTIDDKSYSELSNTYNITDNNEIATKAIRLCLERIRKQTGKSIKH 74
Query 61 WFITELGHNGTERMHLHGLVWGIGMDKLVEEKWQNGIVFTGTFVNEktinyitkyitktD 120
WFITELGH TER+HLHG+VWGIG DKL+ KW GI FTG FVNEKTI YITKY+TK D
Sbjct 75 WFITELGHEKTERLHLHGIVWGIGTDKLITNKWNYGITFTGFFVNEKTIQYITKYMTKID 134
Query 121 EYHKEFIGQILCSAGIGNGYTQRSDAQKHTYKKGETIETYRLRNGAKINLPTYYRNKLFT 180
E HK+FIG++LCS GIG GY +R DA+KHTYK GETIETYRLRNG+KINLP YYRN+LFT
Sbjct 135 EQHKDFIGKVLCSKGIGAGYIKRDDAKKHTYKPGETIETYRLRNGSKINLPIYYRNQLFT 194
Query 181 EEEREKLWIDKIEKGIVWVMGQKVKIDNTEEYEALLEQARHDAIRLQGHQEQNWDKQKYF 240
EEE+E L++DKIEKGI++VMGQKV D+ E Y LL + R RL G+ QNW++QKY
Sbjct 195 EEEKEALFLDKIEKGIIYVMGQKVHRDDEEYYLQLLNEGRKTECRLYGYNLQNWEQQKYL 254
Query 241 krlekqrkkqqkelrkWENHM-------FQSTEECPF 270
RL +Q+K+Q K+L++ E + + E CPF
Sbjct 255 SRLRRQKKRQDKDLQQLETYWALERTKDYTQLETCPF 291
> Alpavirinae_Human_gut_31_037_Microviridae_AG0300_putative.VP4
Length=304
Score = 226 bits (575), Expect = 1e-73, Method: Compositional matrix adjust.
Identities = 125/239 (52%), Positives = 164/239 (69%), Gaps = 0/239 (0%)
Query 1 MSEELRHDPNAYFMTLTFSEEKLEEYKKLCNSEDPNTIATKAMRLMLERCRRKLKHSIKH 60
+SEE+R DPNA F+TLT S+E E+ K K +RL LER R+K K S+KH
Sbjct 56 LSEEVRSDPNAIFVTLTISDESWEKIKNTYIQLSDEDCIKKMVRLFLERVRKKTKKSLKH 115
Query 61 WFITELGHNGTERMHLHGLVWGIGMDKLVEEKWQNGIVFTGTFVNEktinyitkyitktD 120
W TE G TER HLHGL+WG + L + WQ G VF GTFVNE T+NYITKYITKTD
Sbjct 116 WLTTERGGTNTERYHLHGLIWGENTEALTKSLWQYGFVFIGTFVNECTVNYITKYITKTD 175
Query 121 EYHKEFIGQILCSAGIGNGYTQRSDAQKHTYKKGETIETYRLRNGAKINLPTYYRNKLFT 180
+ HK+F LCSAGIG GY RSD++ + +++G+T ETYRLRNG K+NLP YYRNKL+T
Sbjct 176 KKHKDFEPITLCSAGIGKGYLSRSDSELNRFREGKTTETYRLRNGTKLNLPIYYRNKLYT 235
Query 181 EEEREKLWIDKIEKGIVWVMGQKVKIDNTEEYEALLEQARHDAIRLQGHQEQNWDKQKY 239
+EEREKL+++KI+KG VW+ G++ I + + Y +L++ + A +L G +WD KY
Sbjct 236 DEEREKLFLEKIKKGKVWICGRECNIKDWKTYSQILKEEQIKAKQLHGDSPIDWDIAKY 294
> Alpavirinae_Human_feces_B_023_Microviridae_AG0141_putative.VP4
Length=316
Score = 184 bits (466), Expect = 3e-57, Method: Compositional matrix adjust.
Identities = 109/240 (45%), Positives = 156/240 (65%), Gaps = 7/240 (3%)
Query 1 MSEELRHDPNA-YFMTLTFSEEKLEEYKKLCNSEDPNTIATKAMRLMLERCRRKLKHSIK 59
+SEELR A F+TLTF+EE +E + +E N + A+ M E R+K KH+I+
Sbjct 56 LSEELRSGKGAGLFVTLTFNEESYKELAAITKNE--NDMCRLALYRMNENYRQKYKHTIR 113
Query 60 HWFITELGHNGTERMHLHGLVWGIGMDKLVEEKWQNGIVFTGTFVNEktinyitkyitkt 119
HW +TE+G +G R+H+HG++W D +E W+ G ++ G FVNE+TI YITKY+ K
Sbjct 114 HWCVTEIGDDG--RIHIHGIMWCPASD--IERYWKYGYIYIGRFVNEQTILYITKYMLKY 169
Query 120 DEYHKEFIGQILCSAGIGNGYTQRSDAQKHTYKKGETIETYRLRNGAKINLPTYYRNKLF 179
K F ++LCS GIG Y R D++++TY++ T E+Y LR+G KINLP YY+ K++
Sbjct 170 TPVDKNFEPKVLCSKGIGINYLDRLDSKRNTYRENNTDESYMLRSGRKINLPDYYKRKIY 229
Query 180 TEEEREKLWIDKIEKGIVWVMGQKVKIDNTEEYEALLEQARHDAIRLQGHQEQNWDKQKY 239
TEEEREKLWI+K EKG ++MG+KV DN E+ L+E R A L + Q WD++K+
Sbjct 230 TEEEREKLWIEKQEKGYRYIMGEKVSTDNEEKVYKLMEYWRKKAKELYNEKPQEWDREKH 289
> Alpavirinae_Human_feces_A_032_Microviridae_AG0216_putative.VP4
Length=314
Score = 172 bits (436), Expect = 5e-53, Method: Compositional matrix adjust.
Identities = 104/246 (42%), Positives = 157/246 (64%), Gaps = 12/246 (5%)
Query 1 MSEELRHDPNAYFMTLTFSEEK--------LEEYKKLCNSEDPNTIATKAMRLMLERCRR 52
++EEL+++PNA F+TLTF+EE ++ K N + N + A+R LER R+
Sbjct 54 LAEELKNNPNALFITLTFNEENYQRLSWELFKKSKDNLNYTEQNEMCKTAVRRWLERIRK 113
Query 53 KLKHSIKHWFITELGHNGTERMHLHGLVWGIGMDKLVEEKWQNGIVFTGTFVNEktinyi 112
K K SI+HW +TE G + R+HLHG+VW K E+W G + G +VNE TI Y+
Sbjct 114 KTKKSIRHWMVTEKGEDYG-RIHLHGIVW---CPKERIEQWGYGYTYIGDYVNETTIAYV 169
Query 113 tkyitktDEYHKEFIGQILCSAGIGNGYTQRSDAQKHTYKKGETIETYRLRNGAKINLPT 172
TKY+ K E +F G+++CSAGIG Y +A+++ Y+ ET ETY+L NG ++ LP
Sbjct 170 TKYMLKICEKWPDFRGKVMCSAGIGCRYETSYNAKRNRYRGKETKETYKLENGRELPLPK 229
Query 173 YYRNKLFTEEEREKLWIDKIEKGIVWVMGQKVKIDNTEEYEALLEQARHDAIRLQGHQEQ 232
YY +K++TEEEREKLWI K E+G ++ G+KV DN EE++ L + + A +L G + +
Sbjct 230 YYHDKIYTEEEREKLWIIKQERGYRYIAGEKVSTDNLEEWDNLTKYYQKRAEQLYGDKPE 289
Query 233 NWDKQK 238
+W+++K
Sbjct 290 DWEREK 295
> Alpavirinae_Human_gut_30_040_Microviridae_AG0139_putative.VP4
Length=376
Score = 167 bits (423), Expect = 2e-50, Method: Compositional matrix adjust.
Identities = 105/241 (44%), Positives = 142/241 (59%), Gaps = 7/241 (3%)
Query 3 EELRHDPNAYFMTLTFSEE---KLEEYKKLCNSEDPNTIATKAMRLMLERCRRKLKHSIK 59
EE H YF TLT +E K+E L E+PN IATKA+RL LER R+ SI+
Sbjct 57 EEEMHSNFGYFTTLTIDQENIKKIESITGLKWEENPNEIATKALRLFLERTRKDTGKSIR 116
Query 60 HWFITELGHNGTERMHLHGLVWGIGMDKLVEEKWQNGIVFTGTFVNEktinyitkyitkt 119
HW +TELG R+HLHG+ +G +L+ + W G +F G + N K+INYITKY+ K
Sbjct 117 HWCVTELGEKNN-RIHLHGIFFGQKSAELIRKHWNYGFIFIGGYCNSKSINYITKYMLKV 175
Query 120 DEYHKEFIGQILCSAGIGNGYTQRSDA--QKHTYKKGETIETYRLRNGAKINLPTYYRNK 177
D H EF +L S+GIG GY R D QK YK + TY RNG K+ +P YY+NK
Sbjct 176 DIKHPEFKQIVLASSGIGKGYIDRLDYLWQKQNYK-NINVATYTFRNGTKMAMPKYYKNK 234
Query 178 LFTEEEREKLWIDKIEKGIVWVMGQKVKIDNTEEYEALLEQARHDAIRLQGHQEQNWDKQ 237
+FTE+EREK+WI+ + +G++W+ G+KVK D+ E + L E + G W+
Sbjct 235 IFTEKEREKMWINNLNRGLLWIYGEKVKADDWETIDNLREYWQKYGRETMGDNPIAWNAM 294
Query 238 K 238
K
Sbjct 295 K 295
> Alpavirinae_Human_feces_A_016_Microviridae_AG002_putative.VP4
Length=355
Score = 147 bits (370), Expect = 6e-43, Method: Compositional matrix adjust.
Identities = 98/233 (42%), Positives = 137/233 (59%), Gaps = 10/233 (4%)
Query 3 EELRHDPNAYFMTLTFSEEKLEEYKKLCNSEDPNTIATKAMRLMLERCRRKLKHSIKHWF 62
E+LR P A F T T S E+ + K+ N + N I TK RL LER R++ S+KHW
Sbjct 58 EQLRETPTAIFFTGTVSPERYDYIKEKYNLKTDNEIITKIHRLFLERIRKETGKSMKHWC 117
Query 63 ITELGHNGTERMHLHGLVW---GIGMDKLV---EEKWQNGIVFTGTFVNEktinyitkyi 116
+TE GH T R+HLHG+ + G+ KL+ W +G + G + NEKTINY++KY+
Sbjct 118 VTEKGHTNTRRIHLHGIFYAPNGMTQFKLINILRNNWIDGYCYNGKYCNEKTINYVSKYM 177
Query 117 tktDEYHKEFIGQILCSAGIGNGYTQRSDAQKHTYKKGETIETYRLRNGAKINLPTYYRN 176
TK D + E+ G++LCS G+G GY +R ++H + + T E Y R G I LP YY+
Sbjct 178 TKKDMDNPEYTGKVLCSPGLGAGYVKRI-GKRHEWNEENTKEDYYTRQGTYIALPKYYKY 236
Query 177 KLFTEEEREKLWIDKIEKGIVWVMGQKVKIDNTEE---YEALLEQARHDAIRL 226
KLFTE++RE+LWI + G +V KVKI + E Y L +Q D IR+
Sbjct 237 KLFTEDQREQLWIYRENSGEKFVGNFKVKITDEESEEYYNVLKKQHNEDGIRI 289
Lambda K H a alpha
0.318 0.134 0.417 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 22109948